Download or read book Analysis of the DNA Binding Domain of the Herpes Simplex Virus Type 1 UL9 Protein written by Margaret Isabel Arbuckle and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of the Intranuclear Localization of Essential Herpes Simplex Virus Type 1 Replication Proteins written by Christopher Joseph Lukonis and published by . This book was released on 1998 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Functional Significance of the Physical Interaction Between the Herpes Simplex Virus Type 1 Origin binding Protein UL9 and the DNA Polymerase Processivity Factor UL42 written by Kelly S. Trego and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The origin (ori)-binding protein of herpes simplex virus type 1 (HSV-1), encoded by the UL9 open-reading frame, has been shown to physically interact with a number of cellular and viral proteins, including three HSV-1 proteins (ICP8, UL42, and UL8) essential for ori-dependent DNA replication. In this report, it is demonstrated for the first time that the DNA polymerase processivity factor, UL42 protein, provides accessory function to the UL9 protein, by enhancing the 3' to 5' helicase activity of UL9 on partially duplex non-specific DNA substrates. UL42 fails to enhance the unwinding activity of a non-cognate helicase, suggesting enhancement of unwinding requires the physical interaction between UL42 and UL9. UL42 increases the steady-state rate for unwinding a 23/38-mer by UL9, but only at limiting UL9 concentrations, consistent with a role in increasing the affinity of UL9 for DNA. Optimum enhancement of unwinding was observed at UL42:UL9 molecular ratios of 4:1, although enhancement was reduced when high ratios of UL42:DNA were present. Under the assay conditions employed, UL42 did not alter the rate of dissociation of UL9 from the DNA substrate. UL42 also did not alter the requirement or time for an assembly/conformational change step, regardless of whether it was added to DNA prior to or at the same time as UL9, or after steady-state unwinding by UL9 alone had been achieved. Thus, the increased affinity of UL9 for DNA most likely is the result of an increase in the association rate constant for binding of UL9 to DNA, and explains why helicase enhancement is observed only at subsaturating concentrations of UL9 with respect to DNA. Consistent with this interpretation are results which demonstrate that UL42 also enhances the ATPase activity of UL9 on single-strand and partially duplex DNA substrates when UL9 is limiting. In contrast, ICP8 enhances unwinding at both saturating and subsaturating UL9 concentrations, and reduces or eliminates the lag period. The different means by which ICP8 and UL42 enhance activities of UL9 suggest that these two members of the presumed functional replisome may act synergistically on UL9 to effect initiation of HSV-1 DNA replication in vivo.
Download or read book Structure function Analysis of the Herpes Simplex Virus Type 1 Origin Binding Protein UL9 written by Ajay Kumar Malik and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book A Mutational Analysis of the DNA Binding Domain of the Herpes Simplex Virus Immediate Early Protein Vmw 175 written by Kathryn Elizabeth Allen and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Mutational Analysis of the DNA Binding Domain of the Herpes Simplex Virus Immediate Early Protein Vmw 175 written by Kathryn Elizabeth Allen and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Binding Domain of the Herpes Simplex Virus Regulatory Protein ICP4 written by Chin-Lee Wu and published by . This book was released on 1991 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Further Characterization of the UL37 Protein of Herpes Simplex Virus Type 1 and Its Interaction with ICP8 the Major DNA binding Protein of Herpes Simplex Virus written by Allen G. Albright and published by . This book was released on 1994 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mutational and Fluorescence Analysis of a Transcriptional Activation Domain of the VP16 Protein of Herpes Simplex Virus written by Susan M. Sullivan and published by . This book was released on 1998 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of the UL37 Protein of Herpes Simplex Virus Type 1 and Demonstration that it Interacts with ICP8 the Major DNA Binding Protein of Herpes Simplex Virus written by Lisa S. G. Shelton and published by . This book was released on 1992 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Physical Analysis of the UL42 Protein Instrumental in Herpes Simplex Virus Type 1 DNA Replication written by William H. Novak and published by . This book was released on 1995 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Host Proteins Involved in Herpes Simplex Virus Type 1 DNA Synthesis written by Whitney Cogswell and published by . This book was released on 2008 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Herpes simplex virus type 1 (HSV-1) is the prototypic member of a large family of viruses known as Herpesviridae. Herpesviruses possess a double-stranded DNA genome and infect a broad range of species. In addition to HSV-1, there are seven other herpesviruses that are known to infect humans. Although antiviral treatment is available for HSV infections, there are few or no options for treatment of infections by other herpesviruses. One of the best targets for development of new antivirals is the viral DNA replication apparatus. HSV-1 encodes for seven proteins directly involved in viral DNA replication. However, no HSV-1 origin-dependent DNA replication has been reconstituted in vitro using purified viral proteins. It is hypothesized that proteins (host and/or viral) in addition to the seven known viral DNA replication proteins are involved in viral DNA replication in vivo. Moreover, it is predicted that such proteins would interact with one or more of the viral DNA replication proteins. In an effort to identify interacting host proteins, co-immunoprecipitation analysis was performed using antibodies specific for an essential HSV DNA replication protein, UL42, the polymerase processivity factor. Extracts from infected and mock-infected cells were immunoprecipitated with the UL42 antibody. UL42, and all that is bound to it, bind to the antibody which binds to the Protein G agarose matrix. Those proteins that are specifically pulled down from infected cell extracts, but not from mock-infected extracts, were to be identified by mass-spectrometry. The immunoprecipitates were analyzed following separation on denaturing polyacrylamide gels. Gels were silver-stained or analyzed for the presence of UL42 using a Western blot. Conditions were adjusted to optimize the amount of UL42 immunoprecipitated, but UL42 could not be immunoprecipitated quantitatively. Following immunoprecipitation under optimum conditions, no differences were observed between the proteins pulled-down from mock- or infected-cell extracts. Due to the poor ability of the antibody to pull-down UL42, a second approach was employed. A histidine-tagged version of UL42 was purified using a nickel chelating column and then mixed with infected or mock-infected cell extracts. Those proteins that specifically interact with his-UL42 were expected to bind to a nickel column. However, nonspecific interactions with the matrix were observed and prevented identification of proteins by mass-spectrometry.
Download or read book Herpes Simplex Virus 1 DNA Packaging written by Ying Fan and published by . This book was released on 1997 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Investigation of the DNA binding Properties of the Herpes Simplex Virus Type 1 Regulatory Protein ICP4 written by Patricia Ann Kattar-Cooley and published by . This book was released on 1988 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Herpes Simplex Virus New Insights for the Healthcare Professional 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Herpes Simplex Virus. The editors have built Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Herpes Simplex Virus in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Herpes Simplex Virus: New Insights for the Healthcare Professional: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book A Structural and Functional Analysis of Herpes Simplex Virus Type 1 Glycoprotein L an Essential Component of the Viral Fusogenic Complex written by Michael J. Novotny and published by . This book was released on 1996 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
