Download or read book Analysis of Oligosaccharides Via Ion Mobility Mass Spectrometry written by Christian Manz and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of Oligosaccharides and Peptides Using External Source Fourier Transform Mass Spectrometry written by James Alan Carroll and published by . This book was released on 1995 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Influence of Multivalent Metal Cations on Carbohydrate Separations and Fragmentation Patterns Via Ion Mobility Mass Spectrometry written by Kelsey Anne Morrison and published by . This book was released on 2019 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrates, oligosaccharides, and glycans occupy a corner of biopolymers that can be overlooked in the shadow of proteins and nucleic acids but are nonetheless essential for the survival of biological organisms. However, unlike other biological macromolecules, known carbohydrate units have largely homogeneous elemental compositions, where species are instead distinguished by variations in stereochemistry and branching patterns. Because this creates a scenario where complex biological samples may contain isomeric mixtures which cannot be differentiated by mass isolation, incorporating ion mobility separations in front of mass spectrometry can provide another dimension of information to distinguish among isomers. Yet another angle for enhancing glycan analysis with ion mobility-mass spectrometry is by leveraging interactions between carbohydrate species and metal cations, which can form adducts that have altered gas-phase structures relative to protonated or deprotonated ions. In some situations, metal adduction exaggerates the differences in effective size among isomers and thereby facilitates isomeric glycan separation in the drift time space.This dissertation is focused on conveying the utility of metal-glycan interactions for improving the ion mobility-mass spectrometric analysis of carbohydrate species while also describing some complementary experiments that were pursued in support of glycan analysis. Of note, ultraviolet photodissociation (UVPD) with metal-enhanced fragmentation and Fourier multiplexing of ion mobility-ion trap mass spectrometry contributed well to isomeric mixture analysis. Divalent metal cations including select transition metals and alkaline earth metals were evaluated for their potential to enhance drift time separations, with transition metals proving particularly useful in this regard for mixture analysis. Preliminary oligosaccharide analysis with divalent metal species found that carbohydrates will form both singly-charged monomeric metal-glycan adducts as well as doubly-charged dimeric metal-glycan adducts where both appear at the same m/z value. These dimers were subsequently scrutinized for the potential to form heterodimeric metal-glycan dimers wherein two different isomeric glycans could form a single dimer during a mixture analysis without mobility separation and form chimeric mass spectra. Through a combination of isotopic labeling and ion mobility-mass spectrometry, it was shown that heterodimeric metal-glycan species will form, and that mobility separation is vital for analyzing glycan mixtures.

Download or read book Ion Mobility Spectrometry Mass Spectrometry written by Charles L. Wilkins and published by CRC Press. This book was released on 2010-12-14 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analytical power of ion mobility spectrometry-mass spectrometry (IMS-MS) instruments is poised to advance this technology from research to analytical laboratories. Exploring these developments at this critical juncture, Ion Mobility Spectrometry-Mass Spectrometry: Theory and Applications covers the tools, techniques, and applications involved w

Download or read book Sweet and Salty written by Anna J. Taylor and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structural elucidation of underivatized isomeric carbohydrates has continued to be a challenge for the field of mass spectrometry. Human milk oligosaccharides (HMOs) have functions that range from their roles as prebiotics to their importance in neonatal brain development. Differentiation of HMO isomers by tandem mass spectrometry (MS/MS) remains a challenge because structural diversity. Previous research has shown that electron transfer dissociation (ETD) of a doubly charged metal cation adducted oligosaccharide can be separated using ion mobility (IM) and then fragmented by subsequent vibrational activation (VA) to produce unique fragments and dissociation pathways. This work explores the use of electron transfer0́3ion mobility0́3vibrational activation (ET-IM-VA) and targeted MSn experiments via sustained off-resonance irradiation-collision induced dissociation (SORI-CID) to study dissociation pathways of group II metal adducted sialyllacto-N-tetraose (LST) isomers.This dissertation describes the in-depth study of how energy resolved, electron transfer, ion mobility, and subsequent vibration activation methods can be used to investigate the fragmentation of LST isomers and explore the effects of group II metal cation adduction. ET-IM-VA was used to generate unique fragmentation pathways for each of the LST isomers as their group II metal adducts. Differences in relative fragment ion abundance was found to differentiate key structural features, such different linkages. Ion mobility experiments explored how charge state and group II metal adduction affected LST isomer arrival time distributions. The charge reduced LST and group II metal adducted species from the ion/ion reaction with nitrosobenzene radical anion were evaluated and found to be primarily proton transfer products instead of electron transfer products. Understanding fragmentation of native oligosaccharides remains underexplored, but with these studies, we have shown that a multidimensional approach can be utilized to differentiate model acidic oligosaccharide structures. The methods describe in this dissertation provide ways in which isomeric sialylated human milk oligosaccharides can be differentiated, which would benefit future studies of infant health and development.

Download or read book Characterization of Protein bound Oligosaccharides Using Matrix assisted Laser Desorption ionization Fourier Transform Mass Spectrometry written by Hyun Joo An and published by . This book was released on 2004 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Oligosaccharide Analysis Via Anion Attachment Using Negative Mode Electrospray ES and Matrix Assisted Laser Desorption ionization MALDI Mass Spectrometry written by Yanjie Jiang and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Eleven tested anions were able to form adducts with neutral oligosaccharides at low cone voltage in negative ion mode electrospray mass spectrometry. Among them, fluoride and acetate have the abilities to significantly enhance the absolute abundance of [M-H]- for neutral oliogosaccharides. The chloride adduct has the best stability among all the adduct species investigated. For the above three anions, CID of adduct species may be used for structural determination of neutral oligosaccharides. In the presence of F- and Ac-, simultaneous detection of acidic oligosaccharides and neutral oligosaccharides was achieved. The ratio of Cl- : non-Cl-containing product ions obtained in CID spectra of chloride adducts of disaccharides was used to differentiate anomeric configurations of disaccharides. Density functional theory (DFT) was employed to evaluate the optimized structures of chloride adducts of disaccharides. The formation and decomposition of chloride adducts with oligosaccharides of different acidities were investigated in MALDI mass spectrometry.

Download or read book Analysis of Oligosaccharides by Matrix assisted Laser Desorption ionization Fourier Transform Mass Spectrometry written by Mark Thomas Cancilla and published by . This book was released on 1999 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Synthetic Oligosaccharides written by Pavol Kováč and published by . This book was released on 1994 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reviews the use of synthetic oligosaccharides in biochemistry, immunology, pharmaceutical and medicinal chemistry. Presents strategies for synthesis of complex oligosaccharides. Discusses the mechanisms of interaction between carbohydrates and biologically important proteins, such as enzymes, immunoglobulins, and receptor proteins. Valuable reading for biochemists, immunologists, pharmaceutical chemists, organic synthetic chemists, pharmacologists, and researchers in the life sciences.

Download or read book Differential Ion Mobility Spectrometry written by Alexandre A. Shvartsburg and published by CRC Press. This book was released on 2008-12-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction. The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation and first-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods.

Download or read book Assessing Isomeric Heterogeneity of Carbohydrates by Ion Mobility Mass Spectrometry IMMS written by Hongli Li and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: IMMS analysis is fast, sensitive, universal, is capable of resolving isomers and is able to provide unique mobility identities and valuable MS n data on the basis of mobility resolved species including precursor and product ions. Ultimately, IMMS could serve as a novel tool for high throughput identification of glycoprotein.

Download or read book Gas Phase IR Spectroscopy and Structure of Biological Molecules written by Anouk M. Rijs and published by Springer. This book was released on 2015-06-03 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: The series Topics in Current Chemistry presents critical reviews of the present and future trends in modern chemical research. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. Review articles for the individual volumes are invited by the volume editors. Readership: research chemists at universities or in industry, graduate students.

Download or read book Advances in Ion Mobility Mass Spectrometry Fundamentals Instrumentation and Applications written by and published by Elsevier. This book was released on 2019-01-10 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion Mobility Spectrometry, Volume 83 will focuses on new trends, methods and instrumentation in the field, starting from the innovations of each technique, to the most progressive challenges of IM-MS. Chapters includes section on Recent advances in IM-MS, IM-MS Principles and Theory, IM-MS Applications and Instrumentation, and the Future of IM-MS. Presents the latest advancements in IM-MS that are essential for new applications Helps readers understand the state-of-the-art in the currently available IM-MS interfaces and their principle uses Provides information on different IM-MS instrumentation Delves into key applications of IM-MS

Download or read book Ion Mobility Mass Spectrometry written by Frank Sobott and published by Royal Society of Chemistry. This book was released on 2021-11-26 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive guide to the technique, each chapter is written by an internationally recognised expert and will help the end users understand the practicalities of using different instruments for different ion mobility purposes.

Download or read book Carbohydrate Antigens written by Per J. Garegg and published by . This book was released on 1993 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Developed from a symposium at the Fourth Chemical Congress of North America (202nd National Meeting of the ACS) in New York City, August 1991, chapter-papers present research on topics including how proteins recognize and bind oligosaccharides, synthesis and immunological properties of glycopeptide T-cell determinants, Vibrio cholerae polysaccharide studies, and purification of oligosaccharide antigens by weak affinity chromatography. Annotation copyright by Book News, Inc., Portland, OR

Download or read book Growth of the World Tanker Fleet in 1956 nineteen Hundred and Fifty six with Special Reference to the Suez Crisis Prelim Report written by and published by . This book was released on 1956 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mass Spectrometry written by Michael A. Madson and published by Elsevier. This book was released on 2016-05-06 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis—arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules. As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study. Authored by an analytical chemist with more than 30 years of experience in research and industry Serves as a quick reference in mass spectral analysis and carbohydrates Includes more than 60 figures to aid in the retention of key concepts