Download or read book An In Vitro Model for the Study of Platelet Vessel Wall Interactions Following a Freeze Thaw Injury written by Lynn R. Trusal and published by . This book was released on 1983 with total page 30 pages. Available in PDF, EPUB and Kindle. Book excerpt: Platelet-endothelial cell interactions are important for maintaining normal hemodynamics. The intact endothelial cell lining is considered non-thrombogenic, but following disruption of the lining platelets will bind to the subendothelium. There is also much conjeture concerning the affinity of platelets for damaged endothelial cells. A model is described for the study of platelet-arota vessel wall interactions following freeze-thaw insult. Using this model, control aortas (37C) perfused with platelet rich palmsa (PRP) or gel filtered platelets did adhere to areas of exposed subendothelium. Following freeze-thaw insult ( -15C of -20C), the endothelial lining was grossly disrupted. The remaining endothelium was severely damaged, demonstrating holes and pits in the plasma membranes and separation of adjacent cell borders. Platelets readily adhere to the subendothelium, but were rarely noted in sole contact with the damaged endothelium. Platelet binding did not result in morphological changes, degranulation or aggregation. Using transmission electron microscopy, platelets were noted in contact with amorphous material and microfibrils but not collagen fibers of the subehdothelium. It is concluded that this model is suitable for the in vitro study of certain hemodynamic phenomena associated with blood vessel freeze-thaw injury. In addition, freeze-thaw damage in this in vitro model, indicated that platelet-vessel wall interactions were limited to areas of exposed subendothelium.
Download or read book Platelet Endothelial Cell Interactions Following Freeze Thaw Injury Or Detergent Treatment to Cultured Cells In Vitro written by Lynn R. Trusal and published by . This book was released on 1982 with total page 26 pages. Available in PDF, EPUB and Kindle. Book excerpt: Platelet interactions with cultured bovine endothelial cells were studied following freeze-thaw damage or detergent treatment. Platelets from whole blood (WB), platelet rich plasma (PRP) or gel filtered (GFP) did not interact directly with freeze-thaw damage endothelial cells. Freezing and thawing did result in the exposure of an extracellular matrix (ECM) located beneath the cells, which proved very thrombogenic. Platelets from all sources attached to both microfilament and amorphous components of the ECM, although only platelets from WB demonstrated aggregation and extensive pseudopodia formation. Treatment of cells with Triton-X detergent resulted in exposure of a intracellular cytoskeleton. Most platelets attached to the cytoskeleton were located near the cell border and had one or more pseudopodia either in contact with extracellular material or penetrating the cytoskeleton. Adhesion of platelets to ECM may represent platelet-collagen or platelet-fibronectin interactions since both are produced by and incorporated into the ECM. Platelet interaction with endothelial cytoskeletons probably represented pseudopodia contact with the now exposed ECM located beneath the endothelial cells. The possibility that platelets also adhered to intracellular components could not be eliminated. These findings are in agreement with data from an isolated aorta freeze-thaw injury model.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Medicine Biology written by and published by . This book was released on 1983-07-26 with total page 34 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Hemostasis and Thrombosis written by Robert W. Colman and published by . This book was released on 1987 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Government Reports Annual Index written by and published by . This book was released on 1983 with total page 1378 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Government Reports Announcements Index written by and published by . This book was released on 1983 with total page 708 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Interference Problems on Wing fuselage Combinations in Inviscid Incompressible Flow written by Aeronautical Research Council (Great Britain) and published by . This book was released on 1974 with total page 1212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.
Download or read book Federation Proceedings written by Federation of American Societies for Experimental Biology and published by . This book was released on 1982-10 with total page 1868 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nano Biomaterials for the Delivery of Therapeutic and Biological Cues for Regenerative Medicine written by Silvia Minardi and published by Frontiers Media SA. This book was released on 2022-02-21 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemistry of Platelets written by David Phillips and published by Elsevier. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry of Platelets is a comprehensive review of the biochemistry of platelets, with emphasis on the molecular basis for the various biological processes in which they participate. Topics range from stimulus-response coupling mechanisms to platelet contractile proteins, platelet membrane glycoproteins, and storage organelles in platelets and megakaryocytes. The expression and function of adhesive proteins on the platelet surface is also discussed. Comprised of 12 chapters, this book begins with a description of the morphological and metabolic responses to agonists, as well as the involvement of certain processes in the coupling of agonist-receptor interactions to platelet responses. The following chapter deals with platelet arachidonate metabolism and platelet-activating factor, focusing on the release of arachidonate from platelet lipid stores; pathways of platelet arachidonate metabolism and effects of arachidonate metabolites; and inhibition of platelet arachidonate metabolism by aspirin. The structure, function, and modification in disease of platelet membrane glycoproteins are then discussed, along with prothrombin activation on platelets and platelet regulation of thrombus formation. Secreted platelet proteins as markers for pathological disorders are also considered. This monograph is intended as a reference for investigators involved in platelet research as well as a source of information for those working in other areas of biological investigation.
Download or read book Nanovaccinology written by Kaushik Pal and published by Springer Nature. This book was released on 2023-09-23 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an overview of the cumulative work on a driving force for innovation in medicine and modern healthcare, boosting advances in therapeutics, biosensors, vaccines, and clinical systems. The work presented shows how nanoparticles have been investigated as vaccine adjuvants because they possess chemical and structural properties that improve immunogenicity as well as the use of nanotechnology in the construction of immunization systems that has developed into the field of viral nanovaccinology. The volume highlights potential areas of research, innovation, and development of finished products for future commercialization and significant research exploration through nanoparticles that prove capable of surmounting most of the barriers like toxicity issues, clearance from biological system, DNA instability, and differences in expression systems. The contributing authors review the primary literature on principles, limitations, and recent breakthroughs in nanoparticle-based antigen delivery vehicles, their use in different diseases, the major bottlenecks, and related issues. Highlighting advances in nanoparticle engineering and the understanding of nanoparticle characteristics as well as critical legacy work dome in the field of nanobiotechnology, the book is ideal for a range of researchers and students in the pure and applied sciences devoted to nanomaterials, vaccinology, and translational medicine.
Download or read book Principles and Practice of Fertility Preservation written by Jacques Donnez and published by Cambridge University Press. This book was released on 2011-02-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: The specialty of fertility preservation offers patients with cancer, who are rendered infertile by chemo- and radiotherapy, the opportunity to realize their reproductive potential. This gold-standard publication defines the specialty. The full range of techniques and scientific concepts is covered in detail, and the author team includes many of the world's leading experts in the field. The book opens with introductions to fertility preservation in both cancer and non-cancer patients, followed by cancer biology, epidemiology and treatment, and reproductive biology and cryobiology. Subsequent sections cover fertility preservation strategies in males and females, including medical/surgical procedures, ART, cryopreservation and transplantation of both ovarian tissue and the whole ovary, and in-vitro follicle growth and maturation. Concluding chapters address future technologies, as well as ethical, legal and religious issues. Richly illustrated throughout, this is a key resource for all clinicians specializing in reproductive medicine, gynecology, oncology, hematology, endocrinology and infertility.
Download or read book Freezing Colloids Observations Principles Control and Use written by Sylvain Deville and published by Springer. This book was released on 2017-01-19 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive overview of the freezing of colloidal suspensions and explores cutting-edge research in the field. It is the first book to deal with this phenomenon from a multidisciplinary perspective, and examines the various occurrences, their technological uses, the fundamental phenomena, and the different modeling approaches. Its chapters integrate input from fields as diverse as materials science, physics, biology, mathematics, geophysics, and food science, and therefore provide an excellent point of departure for anyone interested in the topic. The main content is supplemented by a wealth of figures and illustrations to elucidate the concepts presented, and includes a final chapter providing advice for those starting out in the field. As such, the book provides an invaluable resource for materials scientists, physicists, biologists, and mathematicians, and will also benefit food engineers, civil engineers, and materials processing professionals.
Download or read book Platelets and Megakaryocytes written by Jonathan M. Gibbins and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: 12 The average human body has in the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.
Download or read book The Commercial Storage of Fruits Vegetables and Florist and Nursery Stocks written by Robert E. Hardenburg and published by . This book was released on 1986 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Note for the electronic edition: This draft has been assembled from information prepared by authors from around the world. It has been submitted for editing and production by the USDA Agricultural Research Service Information Staff and should be cited as an electronic draft of a forthcoming publication. Because the 1986 edition is out of print, because we have added much new and updated information, and because the time to publication for so massive a project is still many months away, we are making this draft widely available for comment from industry stakeholders, as well as university research, teaching and extension staff.
