Download or read book Adsorption of Trichoderma Reesei CBHI and Thermomonospora Fusca E5 Cellulases on Model Solid Surfaces written by Carolyn S. Baker and published by . This book was released on 1998 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this research, the interfacial behavior of Trichoderma reesei CBHI and Thermomonospora fusca E5 cellulases were studied at synthetic surfaces. For this purpose, colloidal silica and polystyrene particles were used to prepare cellulase-particle suspensions that were analyzed by several solution-phase techniques. These included circular dichroism spectroscopy, size exclusion chromatography and filtration, and a spectrophotometric assay for cellulase activity. All techniques were performed in the presence and absence of particles. Circular dichroism spectroscopy (CD) and size exclusion chromatography showed, however, that binding did not occur between either cellulase and silica, presumably because silica is hydrophilic and negatively charged. Binding did occur between each cellulase and polystyrene, most likely mediated through hydrophobic associations. Cellulase-polystyrene complexes were not analyzed using CD because of high light absorption by the polystyrene nanoparticles. Upon adsorption to polystyrene, the activity of the E5 dropped about 95% relative to that of the free enzyme. While this substantial loss in activity may have been the result of binding being mediated through the catalytic domain, strong evidence supporting the thought that adsorption occurs through hydrophobic associations, mediated through the binding domain, suggests that structural or steric factors were partly responsible for the loss.
Download or read book Adsorption of Thermomonospora Fusca E3 and E5 and Trichoderma Reesei CBHI Cellulases on Cellulose and Silica written by Worakrit Suvajittanont and published by . This book was released on 1999 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Assaying the Activities of Thermomonospora Fusca E5 and Trichoderma Reesei CBHI Cellulases Bound to Polystyrene written by Sasithorn Kongruang and published by . This book was released on 1999 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this study the enzymatic activity of adsorbed Thermomonospora fusca E5 and Trichoderma reesei CBHI cellulases were investigated using fluorescence techniques. In particular, cellulases were allowed to contact hydrophobic polystyrene surfaces under conditions of different solution concentrations, and adsorption times. Each of these variables is known to have a potential effect on enzyme structure and activity at an interface. Enzymatic activity was measured after partial elution of the adsorbed layer with both protein-free buffer and the surfactant, dodecyltrimethylammonium bromide. For E5 at high concentration (0.5 mg/ml), adsorbed enzyme activity decreased about 20% in increasing adsorption time from 0.25 h to 24 h. At low concentration (0.001 mg/ml), adsorbed enzyme activity decreased by one order of magnitude during a 24 h period. CBHI layers lost activity only after a sufficiently long contact time with the surface, and this effect was not strongly dependent on enzyme concentrations in solution. These findings were explained with reference to structural changes undergone by adsorbed enzyme as a function of time and available interfacial area.
Download or read book Binding Kinetics of Thermomonospora Fusca E3 and E5 and Trichoderma Reesei CBHI written by Michelle Kay Bothwell and published by . This book was released on 1994 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals written by Charles E. Wyman and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biomass is attracting increasing attention as a sustainable resource for large-scale production of renewable fuels and chemicals. However, in order to successfully compete with petroleum, it is vital that biomass conversion processes are designed to minimize costs and maximize yields. Advances in pretreatment technology are critical in order to develop high-yielding, cost-competitive routes to renewable fuels and chemicals. Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals presents a comprehensive overview of the currently available aqueous pretreatment technologies for cellulosic biomass, highlighting the fundamental chemistry and biology of each method, key attributes and limitations, and opportunities for future advances. Topics covered include: • The importance of biomass conversion to fuels • The role of pretreatment in biological and chemical conversion of biomass • Composition and structure of biomass, and recalcitrance to conversion • Fundamentals of biomass pretreatment at low, neutral and high pH • Ionic liquid and organosolv pretreatments to fractionate biomass • Comparative data for application of leading pretreatments and effect of enzyme formulations • Physical and chemical features of pretreated biomass • Economics of pretreatment for biological processing • Methods of analysis and enzymatic conversion of biomass streams • Experimental pretreatment systems from multiwell plates to pilot plant operations This comprehensive reference book provides an authoritative source of information on the pretreatment of cellulosic biomass to aid those experienced in the field to access the most current information on the topic. It will also be invaluable to those entering the growing field of biomass conversion.
Download or read book Proceedings of the Institute of Biological Engineering written by Institute of Biological Engineering and published by . This book was released on 1999 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1999 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2016-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, focusing on industrial biotechnology and bioengineering practices for the production of industrial products, such as enzymes, organic acids, biopolymers, and biosurfactants, and the processes for isolating and purifying them from a production medium. During the last few years, the tools of molecular biology and genetic and metabolic engineering have rendered tremendous improvements in the production of industrial products by fermentation. Structured by industrial product classifications, this book provides an overview of the current practice, status, and future potential for the production of these agents, along with reviews of the industrial scenario relating to their production. - Provides information on industrial bioprocesses for the production of microbial products by fermentation - Includes separation and purification processes of fermentation products - Presents economic and feasibility assessments of the various processes and their scaling up - Links biotechnology and bioengineering for industrial process development
Download or read book Enzyme Biocatalysis written by Andrés Illanes and published by Springer Science & Business Media. This book was released on 2008-06-19 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.
Download or read book Chitooligosaccharides written by Se-Kwon Kim and published by Springer Nature. This book was released on 2022-02-23 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book outlines the production of chitooligossacharides and their derivatives and discusses their main biological activities, biomedical applications and their role in disease prevention. Chitooligosaccharides are products of chitosan or chitin degradation, prepared by enzymatic or chemical hydrolysis of chitosan, and they consist mainly of N-acetyl glucosamine and glucosamine bonded with a glycosidic bond. Compared to chitin and chitosan, chitooligossacharides offer advantages for large-scale and commercial applications due to their solubility in water and lower molecular weight. Written by leading experts, this book is divided into four parts. The first part provides a general introduction to chitooligossacharides. The second part focuses on the bioproduction of chitooligossacharides through enzymatic synthesis and also covers physical and chemical methods of synthesis. The third part explores the major biological activities of chitooligosaccharides, including antioxidant, antimicrobial, anti-allergic, anti-inflammatory, anti-cancer and neuroprotective activities, and discusses the disease preventing mechanisms of chitooligosaccharides. In this section, readers will also find about the latest in vivo studies which support the use of chitooligosaccharides in the prevention and control of disease. The final part highlights important biomedical applications of chitooligosaccharides, including in tissue engineering, drug delivery and wound healing applications. It also includes the volume editor’s perspective on the health and safety risks of chitooligosaccharides. Given its scope, this book is useful not only for researches in the field but also for students interested in biomaterials, pharmaceuticals, marine biotechnology, nutraceuticals and food science.
Download or read book Cellulose Hydrolysis written by Liang-tseng Fan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent economic trends, especially the worldwide decline in oil prices, and an altered political climate in the United States have combined to bring about major reductions in research on renewable energy resources. Yet there is no escaping the "facts of life" with regard to these resources. The days of inexpensive fossil energy are clearly numbered, the credibility of nuclear energy has fallen to a new low, and fusion energy stands decades or more from practical realization. Sooner than we may wish ,we will have to turn to renewable raw materials - plant "biomass" and, especially, wood - as significant suppliers of energy for both industry and everyday needs. It is therefore especially important to have a single, comprehensive and current source of information on a key step in any process for the technological exploitation of woody materials, cellulose hydrolysis. Further more, it is essential that any such treatment be unbiased with respect to the two methods - chemical and biochemical - for the breakdown of cellulose to sugars. Researchers on cellulose hydrolysis have frequently been chided by persons from industry, especially those individuals concerned with determining the economic feasibility of various technological alternatives. They tell us that schemes for the utilization of wood and other such resources fly in the face of economic realities.
Download or read book Secretions and Exudates in Biological Systems written by Jorge M. Vivanco and published by Springer Science & Business Media. This book was released on 2012-01-21 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Secretions and emissions in biological systems play important signaling roles within the organism but also in its communications with the surrounding environment. This volume brings together state-of-the-art information on the role of secretions and emissions in different organs and organisms ranging from flowers and roots of plants to nematodes and human organs. The plant chapters relate information regarding the biochemistry of flower volatiles and root exudates, and their role in attracting pollinators and soil microbial communities respectively. Microbial chapters explain the biochemistry and ecology of quorum sensing and how microbial communities highly co-adapted to plants can aid in bio-energy applications by degrading ligno-cellulosic materials. Other chapters explain the biology of secretions by nematodes, algae and humans, among other organisms. This volume will be a welcome addition to the literature, as no other book covers aspects related to biological secretion in such a holistic and integrative manner.
Download or read book Biofuels written by Jonathan R. Mielenz and published by Humana Press. This book was released on 2012-03-07 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: This editor believes the public finally understands that bioenergy should be a critical new 39 component of our world’s future energy supply. While this new enlightenment might have 40 come from bioenergy’s environmentally friendly attributes and its renewable and sustain- 41 able nature, the reality is that the primary driver has been both the high cost of energy, 42 led recently by petroleum products, and the unique climatic changes such as local weather 43 events and reports of melting of ancient glaciers. I believe that much of the public under- 44 stands the primary cause of these changes is the growing demand for fossil fuels for heat- 45 ing and transportation needs driven by both population increases and dramatic economic 46 development around the globe. This increased demand, as well as global politics, has 47 impacted the costs for these energy products. At the same time, increased use of fossil fuels 48 has been liberating unprecedented levels of CO and other greenhouse gasses (GHGs) 49 2 that must be impacting the global climate. As a result, the world is looking for alternative 50 solutions. Renewable energy is one of the leading options as long as it is pursued in a sus- 51 tainable fashion. Of course, renewable energy has numerous sources, including wind, solar, 52 geothermal, ocean currents/tides, and bio-based energy. The latter category of renewable 53 energy is centered primarily on use of green plant matter, i. e.
Download or read book Handbook of Plant Based Biofuels written by Ashok Pandey and published by CRC Press. This book was released on 2008-09-26 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores Worldwide Trends Involving the Production and Use of Biofuels With the depletion of oil resources as well as the negative environmental impact of fossil fuels, there is much interest in alternative energy sources. Focusing on some of the most important alternate energy sources for the foreseeable future, the Handbook of Plant-Based Biofuels provides state-of-the-art information on the status of the production of biofuels, in particular, bioethanol and biodiesel. Introduction to Biofuels After profiling plant-based biofuels, the book gives an overview of the production of biofuels from biomass materials by thermochemical and biochemical methods. It examines the thermochemical conversion of biomass to liquids and gaseous fuels. Production of Bioethanol The handbook then analyzes current biomass-to-ethanol programs, followed by a discussion on ethanol fermentation from molasses and process practices applied for the improvement of ethanol production by ethanologenic microorganisms. It also explains the hydrolysis and fermentation of ethanol from starchy and lignocellulosic biomasses. Production of Biodiesel In the final chapters, the contributors discuss current perspectives and the future of biodiesel production. They explore biodiesel production substrates, the lipase-catalyzed preparation of biodiesel, and biodiesel production with supercritical fluid technologies.
Download or read book Industrial Enzymes and Their Applications written by Helmut Uhlig and published by John Wiley & Sons. This book was released on 1998-04-06 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: * Ein ma?gebender Katalog technischer Enzyme, ihrer Charakteristika und industriellen Verwendung. * Durch die leicht verstandliche Einfuhrung zu jedem Kapitel sowie die Beschreibung der technischen Enzyme ist dieses Werk auch fur Neulinge auf diesem Gebiet mit fehlendem wissenschaftlichen Hintergrund bestens geeignet. * Enthalten sind Informationen uber konkurrierende Enzymprodukte, so da? der Leser aufgrund dieses Wissens Enzyme fur bestimmte Zwecke auswahlen kann. * Sehr praxisorientiert, mit mehr als 100 Abbildungen, die genau zeigen, wie Enzymcharakteristika entwickelt werden. In jedem Kapitel finden sich zum besseren Verstandnis der technischen Seite Flie?diagramme. * Spezielle Kapitel fur die Leder, Back-, Saft- und Weinindustrie (Die wichtigsten Verbraucher industrieller Enzyme); einschlie?lich einem geschichtlichen Abri? uber die Verwendung von Enzymen in der Industrie * Au?erdem enthalten: Gesetzesvorschriften zum Einsatz industrieller Enzyme. (01/98)
Download or read book Biochemistry of microbial degradation written by Colin Ratledge and published by Springer. This book was released on 2012-10-25 with total page 590 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life on the planet depends on microbial activity. The recycling of carbon, nitrogen, sulphur, oxygen, phosphate and all the other elements that constitute living matter are continuously in flux: microorganisms participate in key steps in these processes and without them life would cease within a few short years. The comparatively recent advent of man-made chemicals has now challenged the environment: where degradation does not occur, accumulation must perforce take place. Surprisingly though, even the most recalcitrant of molecules are gradually broken down and very few materials are truly impervious to microbial attack. Microorganisms, by their rapid growth rates, have the most rapid turn-over of their DNA of all living cells. Consequently they can evolve altered genes and therefore produce novel enzymes for handling "foreign" compounds - the xenobiotics - in a manner not seen with such effect in other organisms. Evolution, with the production of micro-organisms able to degrade molecules hitherto intractable to breakdown, is therefore a continuing event. Now, through the agency of genetic manipulation, it is possible to accelerate this process of natural evolution in a very directed manner. The time-scale before a new microorganism emerges that can utilize a recalcitrant molecule has now been considerably shortened by the application of well-understood genetic principles into microbiology. However, before these principles can be successfully used, it is essential that we understand the mechanism by which molecules are degraded, otherwise we shall not know where best to direct these efforts.
Download or read book Current Developments in Biotechnology and Bioengineering written by Christian Larroche and published by Elsevier. This book was released on 2016-09-17 with total page 852 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Bioprocesses, Bioreactors and Controls provides extensive coverage of new developments, state-of-the-art technologies, and potential future trends, reviewing industrial biotechnology and bioengineering practices that facilitate and enhance the transition of processes from lab to plant scale, which is becoming increasingly important as such transitions continue to grow in frequency. Focusing on industrial bioprocesses, bioreactors for bioprocesses, and controls for bioprocesses, this title reviews industrial practice to identify bottlenecks and propose solutions, highlighting that the optimal control of a bioprocess involves not only maximization of product yield, but also taking into account parameters such as quality assurance and environmental aspects. - Describes industrial bioprocesses based on the reaction media - Lists the type of bioreactors used for a specific bioprocess/application - Outlines the principles of control systems in various bioprocesses
