Download or read book A Study of the Kinetics and Mechanism of the Oxidation of NADH by Ferrocenium Ions and Quinones written by Brian Willard Carlson and published by . This book was released on 1984 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Study of the Kinetics and Mechanism of the Oxidation of NADH by Ferrocenicm Ions and Quinones written by Brian Willard Carlson and published by . This book was released on 1985 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1989 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1985 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioelectrochemistry written by Philip N. Bartlett and published by John Wiley & Sons. This book was released on 2008-05-27 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioelectrochemistry: Fundamentals, Experimental Techniques and Application, covers the fundamental aspects of the chemistry, physics and biology which underlie this subject area. It describes some of the different experimental techniques that can be used to study bioelectrochemical problems and it describes various applications of biolelectrochemisty including amperometric biosensors, immunoassays, electrochemistry of DNA, biofuel cells, whole cell biosensors, in vivo applications and bioelectrosynthesis. By bringing together these different aspects, this work provides a unique source of information in this area, approaching the subject from a cross-disciplinary viewpoint.
Download or read book Doctoral Degree Recipients written by University of Minnesota and published by . This book was released on 1984 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1984 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Register of Doctoral Degrees Conferred by the University of Minnesota written by University of Minnesota and published by . This book was released on 1976 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Preliminary Investigation of the Kinetics and Mechanism of the Oxidation of the Vanadyl Ion by Peroxydiphophate written by Mary Doris Wiswell and published by . This book was released on 1967 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mechanism of NADH Oxidation by Respiratory Complex I with Focus on the Production of Reactive Oxygen Species written by Emmanuel Gnandt and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The NADH:ubiquinone oxidoreductase, respiratory complex I, couples the transfer of electrons from NADH to ubiquinone with the translocation of four protons across a membrane. The mitochondrial complex I consists of 45 subunits, which the homologous bacterial one was shown to be composed of 13-15 subunits. 14 subunits are conserved in all organisms and represent the catalytic core subunits. The structures of the complex of bacteria and mitochondria is virtually identical. Thus, the bacterial enzyme represents a minimal structural model of mitochondrial complex I. Complex I oxidizes NADH by FMN and transfers the electrons to the quinone by a chain of seven (Fe-S)-clusters. An eighth cluster, located proximally to FMN, N1a, is conserved in the complex from all kingdoms of life, but not involved in the electron transfer to quinone. Hence, its role for catalysis remains enigmatic. The NADH oxidation site is susceptible of producing reactive oxygen species due to its solvent exposure. Complex I is known to be the major producer of cellular ROS and its dysfunction is associated with a multitude of severe diseases, such as the Leigh syndrome, a severe neurodegenerative disease. In this work, the function of N1a should be identified by means of site-directed mutagenesis, enzymatic kinetics and structural analysis. Mutations found in patients suffering from Leigh syndrome should be introduced into bacterial complex I to identify the effects on structure and function. Finally, competitors to NADH binding should be identified that selectively inhibit ROS production. Reduction of the peripheral cluster N1a could not be detected even using fast stopped-flow kinetics. However, EPR spectroscopy of samples that were generated by the ultra-fast freeze quenching revealed that the electron tunneling rates in a complex I variant containing N1a that cannot be reduced by NADH do not change. However, reduction of N1a regulates NADH binding. The rate of the complex I catalyzed activity is significantly diminished when the reation is initiated by an addition of ferricyanide instead of NADH. Detailled enzyme kinetics revealed that reduction of the (Fe-S)-clusters leads to a conformational change that in turn leaves the product NAD+ in its binding site blocking the access of NADH. This effect was not detectable in the variant with N1a that is not reducible by NADH. Thus, the reduction of N1a leads to a lower dissociation constant of NAD+. Structural investigations in our group revealed the local structural changes in the NADH binding site due to N1a reduction. Elimination of this mechanism by site-directed mutagenesis leads to an enhanced ROS production. Furthermore, techniques were established to determine the thermal stability of proteins. They are based on the release of a fluorescent cofactor or the binding of a fluorescent marker. Several proteins were characterized by this methods revealing their complementarity. A novel buffer for the purification of complex I was established using these methods. Mutations found in patients suffering from Leigh syndrome were introduced into E. coli complex I and the electron input modul of the compex from Aquifex aeolicus. The consequences of the mutations on the catalytic properties were tested with the E. coli variants and structural consequences were determined in the structure of the A. aeolicus modul. The mutations caused a reduced activity, an increase in H2O2 production, a change of the flavin midpoint potential and a reduced thermal stability of the enzyme, especially at the flavin site. It seems that Leigh syndrome is mainly caused by a reduced enzymatic activity paired with an increased H2O2 production. To discover new structures that are possibly capable to reduce ROS production at the reduced FMN cofactor in complex I a combination of structure-based and fragment-based drug design with the highly specific complex I inhibitor NADH-OH as lead structure was used. Several possible binders were identified and their inhibitory effect on the NADH oxidase activity was determined. The structure of two compounds bound to NuoEF were successfully solved and identified a binding site within the NADH oxidation site. However, these compounds showed a low affinity to the complex
Download or read book A Study of the Products Kinetics and Mechanism of the Cerium IV Oxidation of N benzyliminodiacetic Acid in Acidic Perchlorate and Acidic Sulfate Media written by Marilyn Eileen Moehlenkamp and published by . This book was released on 1981 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetics and Mechanisms of the Oxidation of Alcohols and Hydroxylamines by Hydrogen Peroxide Catalyzed by Methyltrioxorhenium MTO and the Oxygen Binding Properties of Cobalt Schiff Base Complexes written by and published by . This book was released on 1999 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: Catalysis is a very interesting area of chemistry, which is currently developing at a rapid pace. A great deal of effort is being put forth by both industry and academia to make reactions faster and more productive. One method of accomplishing this is by the development of catalysts. Enzymes are an example of catalysts that are able to perform reactions on a very rapid time scale and also very specifically; a goal for every man-made catalyst. A kinetic study can also be carried out for a reaction to gain a better understanding of its mechanism and to determine what type of catalyst would assist the reaction. Kinetic studies can also help determine other factors, such as the shelf life of a chemical, or the optimum temperature for an industrial scale reaction. An area of catalysis being studied at this time is that of oxygenations. Life on this earth depends on the kinetic barriers for oxygen in its various forms. If it were not for these barriers, molecular oxygen, water, and the oxygenated materials in the land would be in a constant equilibrium. These same barriers must be overcome when performing oxygenation reactions on the laboratory or industrial scale. By performing kinetic studies and developing catalysts for these reactions, a large number of reactions can be made more economical, while making less unwanted byproducts. For this dissertation the activation by transition metal complexes of hydrogen peroxide or molecular oxygen coordination will be discussed.
Download or read book Bioinorganic Photochemistry written by Grazyna Stochel and published by John Wiley & Sons. This book was released on 2009-06-10 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioinorganic photochemistry is a rapidly evolving field integrating inorganic photochemistry with biological, medical and environmental sciences. The interactions of light with inorganic species in natural systems, and the applications in artificial systems of medical or environmental importance, form the basis of this challenging inter-disciplinary research area. Bioinorganic Photochemistry provides a comprehensive overview of the concepts and reactions fundamental to the field, illustrating important applications in biological, medical and environmental sciences. Topics covered include: Cosmic and environmental photochemistry Photochemistry of biologically relevant nanoassemblies Molecular aspects of photosynthesis Photoinduced electron transfer in biosystems Modern therapeutic strategies in photomedicine The book concludes with an outlook for the future of environmental protection, discussing emerging techniques in the field of pollution abatement, and the potential for bioinorganic photochemistry as a pathway to developing cheap, environmentally friendly sources of energy. Written as an authoritative guide for researchers involved in the development of bioinorganic photochemical processes, Bioinorganic Photochemistry is also accessible to scientists new to the field, and will be a key reference source for advanced courses in inorganic, and bioinorganic chemistry.
Download or read book Enzymatic Fuel Cells written by Heather R. Luckarift and published by John Wiley & Sons. This book was released on 2014-05-06 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizes research encompassing all of the aspects required to understand, fabricate and integrate enzymatic fuel cells Contributions span the fields of bio-electrochemistry and biological fuel cell research Teaches the reader to optimize fuel cell performance to achieve long-term operation and realize commercial applicability Introduces the reader to the scientific aspects of bioelectrochemistry including electrical wiring of enzymes and charge transfer in enzyme fuel cell electrodes Covers unique engineering problems of enzyme fuel cells such as design and optimization
Download or read book Oxide Surfaces written by and published by Elsevier. This book was released on 2001-05-21 with total page 677 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is a multi-author survey (in 15 chapters) of the current state of knowledge and recent developments in our understanding of oxide surfaces. The author list includes most of the acknowledged world experts in this field. The material covered includes fundamental theory and experimental studies of the geometrical, vibrational and electronic structure of such surfaces, but with a special emphasis on the chemical properties and associated reactivity. The main focus is on metal oxides but coverage extends from 'simple' rocksalt materials such as MgO through to complex transition metal oxides with different valencies.
Download or read book The Activation of Dioxygen and Homogeneous Catalytic Oxidation written by D.H.R. Barton and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph consists of the proceedings of the Fifth International Symposium on the Activation of Dioxygen and Homogeneous Catalytic Oxidation, held in College Station, Texas, March 14-19, 1993. It contains an introductory chapter authored by Professors D. H. R. Barton and D. T. Sawyer, and twenty-nine chapters describing presentations by the plenary lecturers and invited speakers. One of the invited speakers, who could not submit a manuscript for reasons beyond his control, is represented by an abstract of his lecture. Also included are abstracts of forty-seven posters contributed by participants in the symposium. Readers who may wish to know more about the subjects presented in abstract form are invited to communicate directly with the authors of the abstracts. This is the fifth international symposium that has been held on this subject. The first was hosted by the CNRS, May 21-29, 1979, in Bendor, France (on the Island of Bandol). The second meeting was organized as a NATO workshop in Padova, Italy, June 24-27, 1984. This was followed by a meeting in Tsukuba, Japan, July 12-16, 1987. The fourth symposium was held at Balatonfured, Hungary, September 10-14, 1990. The sixth meeting is scheduled to take place in Delft, The Netherlands (late Spring, 1996); the organizer and host will be Professor R. A. Sheldon.
Download or read book Plant Litter written by Björn Berg and published by Springer Science & Business Media. This book was released on 2013-11-19 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the 2nd edition, there have been substantial developments in the field of litter decomposition. This fully revised and updated 3rd edition of Plant Litter reflects and discusses new findings and re-evaluates earlier ones in light of recent research and with regard to current areas of investigation. The availability of several long-term studies allows a more in-depth approach to decomposition patterns and to the later stages of decomposition, as well as to humus formation and accumulation. The latest information focuses on three fields: - the effects of manganese on decomposition and possibly on carbon sequestration, - new findings on decomposition dynamics, and - the new analytical technique using 13C-NMR.
