Download or read book A Practical Guide to ELISA written by D. M. Kemeny and published by Pergamon. This book was released on 1991 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay":- cover .
Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Download or read book The ELISA Guidebook written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Download or read book A Practical Guide to ELISA written by D. M. Kemeny and published by Pergamon. This book was released on 1991 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
Download or read book Immunoassay written by Brian Law and published by CRC Press. This book was released on 2002-09-11 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.
Download or read book The Immunoassay Handbook written by David Wild and published by Newnes. This book was released on 2013-01-21 with total page 1037 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Download or read book ELISA and Other Solid Phase Immunoassays written by D. M. Kemeny and published by John Wiley & Sons. This book was released on 1991-01-08 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.
Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Download or read book A Practical Guide to Monoclonal Antibodies written by J. Eryl Liddell and published by John Wiley & Sons. This book was released on 1991-08-26 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.
Download or read book Fanfares and Finesse written by Elisa Koehler and published by Indiana University Press. This book was released on 2014-02-27 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: “A concise guide linking the history of trumpet to performance . . . includes information on band music, bugle calls, orchestral repertoire, and jazz.” —American Reference Books Annual Unlike the violin, which has flourished largely unchanged for close to four centuries, the trumpet has endured numerous changes in design and social status from the battlefield to the bandstand and ultimately to the concert hall. This colorful past is reflected in the arsenal of instruments a classical trumpeter employs during a performance, sometimes using no fewer than five in different keys and configurations to accurately reproduce music from the past. With the rise in historically inspired performances comes the necessity for trumpeters to know more about their instrument’s heritage, its repertoire, and different performance practices for old music on new and period-specific instruments. More than just a history of the trumpet, this essential reference book is a comprehensive guide for musicians who bring that musical history to life. “A compendium of trumpet history with short, fact-filled chapters. It will serve both amateur and professional musicians alike, and few could read this text without learning something. Fanfares and Finesse is a thorough sampling of trumpet topics, including something of interest for every trumpet player, brass enthusiast, or curious reader.” —Pan Pipes “Trumpet players in a wide variety of situations and at many levels will find a great deal of useful information, presented in a clear, engaging, reader-friendly way yet backed by solid research. While some topics are covered in more depth than others, Koehler’s breadth of vision and thoroughness are commendable . . . For all trumpeters and anyone who teaches them.” —Choice
Download or read book The Skillful Team Leader written by Elisa MacDonald and published by Corwin Press. This book was released on 2013-03-13 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: All teams face hurdles. What distinguishes the skillful team leader from a less effective one is his or her approach in overcoming them. MacDonald offers a skillful approach to team leadership rooted in values, mindset, intelligence, and skill.
Download or read book Basic Concepts in Clinical Biochemistry A Practical Guide written by Vijay Kumar and published by Springer. This book was released on 2018-03-30 with total page 175 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Download or read book ELISA Guidebook The Methods in Molecular Biology written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Download or read book Meet Your Soul written by Elisa Romeo and published by Hay House, Inc. This book was released on 2015-03-03 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this groundbreaking book, therapist and intuitive Elisa Romeo, takes Soul beyond an intellectual concept and into a direct and personal relationship. Pulling from depth psychology, years of practical experience, and touching stories from clients, Elisa brings substance and gravitas—and some levity—to the topic of how to live a Soulful life. This comprehensive, straightforward program contains effective meditations, visualizations, and inquiries to support you on the often messy, winding, and yet amusing path of discovering your Soul in modern times. Meet Your Soul not only tells you how to access your Soul connection but also preps you for some of the common obstacles you’re likely to encounter on your journey. With her kind and informed direction, you’ll learn to: • Understand the crucial distinction between ego, Spirit, and Soul • Create and cultivate a strong spiritual practice • Distinguish your Soul Truth from the beliefs of family, friends, and society • Clearly access the voice of your Soul • Gain awareness of the key Soul contracts in your life and discover your Divine purpose This book helps you meet the oldest, wisest, and most loving part of yourself—the part that gives meaning and purpose to your life. Elisa shows that each of us has the capacity to hear this personal, unique guidance directly. You needn’t look outside yourself to a guru, program, or system; rather you can simply look within.
Download or read book Yeast written by Chris White and published by Brewers Publications. This book was released on 2010-02-01 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast: The Practical Guide to Beer Fermentation is a resource for brewers of all experience levels. The authors adeptly cover yeast selection, storage and handling of yeast cultures, how to culture yeast and the art of rinsing/washing yeast cultures. Sections on how to set up a yeast lab, the basics of fermentation science and how it affects your beer, plus step by step procedures, equipment lists and a guide to troubleshooting are included.
Download or read book Conflict and Catastrophe Medicine written by James Ryan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conflict and Catastrophe Medicine - A Practical Guide provides a framework for use by health professionals visiting a resource-constrained environment. Encompassing problems brought about by local conflict or natural disasters, the book covers preparation, organisation, logistics, treatment of major trauma and medical emergencies, and the special problems of delivering medicine in a hostile environment. Conflict and Catastrophe Medicine - A Practical Guide comprehensively tackles: - self-preparation of health professionals to face a range of medical and related problems which occur in hostile and remote environments; - war and disaster medicine, covering acute management, rehabilitation, reconstruction and prevention; - bridging the fields of medicine, nursing, international relations, history, politics and economics. The book also touches on nutrition, infection, trauma, psychiatry and psychological medicine and training. James Ryan, Leonard Cheshire Professor of Conflict Recovery, Royal Free and University College Medical School, London, UK Peter F Mahoney, Consultant Anaesthetist and Honorary Senior Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Ian Greaves, Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Gavin Bowyer, Consultant in Orthopaedic Surgery, Southampton General Hospital, UK.
