Download or read book The ELISA Guidebook written by John R. Crowther and published by Humana. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that hetero- neous enzyme-linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents, for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme-linked conjugates, substrates and chromogens, plastics technology and design of microwell plates, inst- mentation advances and robotics. However, the principles of the ELISA remain the same. There has been some rearrangement of chapters plus addition of three new ones dealing with charting methods for assessing the indirect ELISA, ruggedness and robustness of tests-aspects of kit use and validation, and internal quality control and external quality management of data, respectively. These reflect the need to control what you are doing with ELISA and to exploit the method to its full extent. I do not apologize for dealing with the same areas in different ways a number of times, as it is imperative that principles are understood to allow planning, operation, and control of ELISA.
Download or read book A Practical Guide to ELISA written by D. M. Kemeny and published by Pergamon. This book was released on 1991 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical and readable handbook is written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay. Basic aspects of immunoassays and assay design are discussed. The explanation of ELISA, describing the reagents used, how to calibrate the equipment and how to interpret results, encourages understanding of this technique. The chapter on "Trouble Shooting" enables the user to identify and rectify problems, thus increasing his knowledge and ability so that the recipe section at the end of the book may be used to full advantage.
Download or read book The Immunoassay Handbook written by David Wild and published by Newnes. This book was released on 2013-01-21 with total page 1037 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Download or read book Basic Concepts in Clinical Biochemistry A Practical Guide written by Vijay Kumar and published by Springer. This book was released on 2018-03-30 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.
Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Download or read book The Skillful Team Leader written by Elisa MacDonald and published by Corwin Press. This book was released on 2013-03-13 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: All teams face hurdles. What distinguishes the skillful team leader from a less effective one is his or her approach in overcoming them. MacDonald offers a skillful approach to team leadership rooted in values, mindset, intelligence, and skill.
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Meet Your Soul written by Elisa Romeo and published by Hay House, Inc. This book was released on 2015-03-03 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this groundbreaking book, therapist and intuitive Elisa Romeo, takes Soul beyond an intellectual concept and into a direct and personal relationship. Pulling from depth psychology, years of practical experience, and touching stories from clients, Elisa brings substance and gravitas—and some levity—to the topic of how to live a Soulful life. This comprehensive, straightforward program contains effective meditations, visualizations, and inquiries to support you on the often messy, winding, and yet amusing path of discovering your Soul in modern times. Meet Your Soul not only tells you how to access your Soul connection but also preps you for some of the common obstacles you’re likely to encounter on your journey. With her kind and informed direction, you’ll learn to: • Understand the crucial distinction between ego, Spirit, and Soul • Create and cultivate a strong spiritual practice • Distinguish your Soul Truth from the beliefs of family, friends, and society • Clearly access the voice of your Soul • Gain awareness of the key Soul contracts in your life and discover your Divine purpose This book helps you meet the oldest, wisest, and most loving part of yourself—the part that gives meaning and purpose to your life. Elisa shows that each of us has the capacity to hear this personal, unique guidance directly. You needn’t look outside yourself to a guru, program, or system; rather you can simply look within.
Download or read book Yeast written by Chris White and published by Brewers Publications. This book was released on 2010-02-01 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast: The Practical Guide to Beer Fermentation is a resource for brewers of all experience levels. The authors adeptly cover yeast selection, storage and handling of yeast cultures, how to culture yeast and the art of rinsing/washing yeast cultures. Sections on how to set up a yeast lab, the basics of fermentation science and how it affects your beer, plus step by step procedures, equipment lists and a guide to troubleshooting are included.
Download or read book Culture and Meaning in Health Services Research written by Elisa J Sobo and published by Routledge. This book was released on 2016-06-16 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Culture and Meaning in Health Services Research is a practical guide to applying interpretive qualitative methods to pressing healthcare delivery problems. A leading medical anthropologist who has spent many years working in applied healthcare settings, Sobo combines sophisticated theoretical insights and methodological rigor with authentic, real-world examples and applications. In addition to clearly explaining the nuanced practice of ethnography and guiding the reader through specific methods that can be used in focus groups or interviewing to yield useful findings, Sobo considers the social relationships and power dynamics that influence field entry, data ownership, research deliverables, and authorship decisions. Crafted to communicate the importance of culture and meaning across the many disciplines engaged in health services research, this book is ideal for courses in such fields as public health and health administration, nursing, anthropology, health psychology, and sociology.
Download or read book Guide to Yeast Genetics Functional Genomics Proteomics and Other Systems Analysis written by and published by Academic Press. This book was released on 2010-02-27 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. - Provides up-to-date methods necessary to study genes in yeast - Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations - Serves as an essential reference for any beginning or experienced researcher in the field
Download or read book A Practical Guide for EdTPA Implementation written by Lisa Barron and published by Information Age Publishing. This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: edTPA is the most widely-used performance assessment for pre-service teachers in the United States, and a requirement in many states for teaching licensure. Through edTPA, teacher candidates demonstrate their effectiveness in different aspects of teaching, including planning, instruction, assessment, analysis of teaching, and use of academic language. This book is a practical guide for anyone involved in edTPA implementation. The chapters are written by experienced teacher educators who are leading successful edTPA programs in their own universities, who are in the field, and involved in the work. They represent diverse teacher preparation programs, each with their own strengths and challenges. This book addresses the challenges of edTPA, while providing practical strategies for educative and thoughtful implementation. Organized into four sections, each section explores a different aspect of edTPA implementation, and provides guidance for leading faculty and teacher candidates through edTPA.
Download or read book A Practical Guide to ELISA written by D. M. Kemeny and published by Pergamon. This book was released on 1991 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Written for the non-specialist and first-time users of ELISA - enzyme-linked immunosorbent assay":- cover .
Download or read book Conflict and Catastrophe Medicine written by James Ryan and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conflict and Catastrophe Medicine - A Practical Guide provides a framework for use by health professionals visiting a resource-constrained environment. Encompassing problems brought about by local conflict or natural disasters, the book covers preparation, organisation, logistics, treatment of major trauma and medical emergencies, and the special problems of delivering medicine in a hostile environment. Conflict and Catastrophe Medicine - A Practical Guide comprehensively tackles: - self-preparation of health professionals to face a range of medical and related problems which occur in hostile and remote environments; - war and disaster medicine, covering acute management, rehabilitation, reconstruction and prevention; - bridging the fields of medicine, nursing, international relations, history, politics and economics. The book also touches on nutrition, infection, trauma, psychiatry and psychological medicine and training. James Ryan, Leonard Cheshire Professor of Conflict Recovery, Royal Free and University College Medical School, London, UK Peter F Mahoney, Consultant Anaesthetist and Honorary Senior Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Ian Greaves, Lecturer in Conflict Medicine, Leonard Cheshire Centre, Royal Free and University College Medical School, London, UK Gavin Bowyer, Consultant in Orthopaedic Surgery, Southampton General Hospital, UK.
Download or read book Kirk Bistner s Handbook of Veterinary Procedures and Emergency Treatment written by Richard B. Ford and published by Elsevier Health Sciences. This book was released on 2011-08-17 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provide expert care for cats and dogs! Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment, 9th Edition covers not only the management of emergency conditions, but also strategies for dealing with hundreds of routine diagnostic and treatment challenges in small animals. Its user-friendly format provides instant access to vital information -- making it an ideal resource in emergency situations -- and it is conveniently organized by both body systems and presenting signs to help you easily reach a diagnosis and determine a treatment plan for all clinical situations. Written by veterinary experts Richard Ford and Elisa Mazzaferro, Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment provides current guidelines for small animal emergency care and the diagnostic procedures most commonly performed in a busy, team-oriented practice. - Step-by-step instructions and illustrations are provided for all major emergency and non-emergency clinical procedures. - A logical, easy-to-use format lists all emergency conditions in alphabetical order, and includes quick reference boxes calling out key information such as clinical tips and cautions. - Clear, concise guidelines help you evaluate clinical signs and laboratory test data. - Clinical algorithms make it easier to identify and treat abnormalities. - Guidelines for assessment and treatment include practical advice and solutions, how to examine the small animal patient using a body systems and problem list approach, and a review of basic diagnostic procedures used in daily practice. - Coverage of toxicological emergencies describes how to manage exposures and poisonings. - A quick reference guide to the management of the emergency patient is conveniently located on the inside cover. - A comprehensive drug formulary makes lookup easy, and includes proprietary names, actions/use of each drug, formulations, recommended dosages, and special precautions, with emergency medications highlighted for fast reference - This all-in-one reference includes practical coverage of emergency procedures, physical assessment in sickness and health, routine and advanced testing procedures, diagnostic tests sampling, preparation, procedures, and interpretation. - Quick Reference boxes include potential causes of each clinical abnormality and associated signs, step-by-step diagnostic plans, and clinical algorithms. - The latest vaccination guidelines include protocols for dogs and cats at low, medium, and high risk of exposure to infectious diseases. - Updated coverage keeps you current with the latest on pain assessment, prevention, and treatment.
