Download or read book A Photo crosslinkable Soy derived Bioink for 3D Bioprinting written by Kyle Schwab and published by . This book was released on 2021 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soy protein isolate (SPI) has become increasingly attractive for tissue engineering purposes because of its abundance in nature (a plant-derived protein), ease of isolation and processing, customizable biodegradability, inexpensive cost, and minimal immunogenicity. Combining SPI with methacrylic anhydride to form soy-methacryloyl (SoyMA) makes it possible to develop a semi-synthetic bioink that can recapitulate in-vivo tissue constructs when extruded by a three-dimensional (3D) bioprinter. Bioinks offer an ideal biological microenvironment for cells and can be photo-crosslinked easily, ensuring cell encapsulation and form fidelity. The long-term goal of this research is to demonstrate that SoyMA bioinks can be synthesized to form a 3D cell culture material with a particular degree of functionalization (DoF). Specifically, I propose to develop SoyMA bioinks that can be used to fabricate scaffolds mimicking the microenvironment of spinal cord tissue using neuronal progenitor cells (pheochromocytoma (PC12) cells) and endothelial cells (EC). Using 3D bioprinting, we will test the ability of these scaffolds to promote cell adhesion, directed spreading, and proliferation. TO control the mechanical properties of SoyMA, we will parametrically vary polymerization conditions such as concentration, DoF, and photo-crosslinking. We will also evaluate and compare cell viability and morphology of cells grown in various stiffnesses of SoyMA scaffolds. Taken together, we will demonstrate how SoyMA bioinks, coupled with 3D bioprinting, can be used to fabricate dynamic and tunable tissue scaffolds.
Download or read book Study on Microextrusion based 3D Bioprinting and Bioink Crosslinking Mechanisms written by Liliang Ouyang and published by Springer. This book was released on 2020-09-02 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive study on microextrusion-based 3D bioprinting technologies for bioinks with various crosslinking mechanisms, chiefly focusing on the bioprinting process and bioink properties to provide readers with a better understanding of this state-of-the-art technology. Further, it summarizes a number of general criteria and research routes for microextrusion-based 3D bioprinting using three experimental studies based on shear-thinning, thermo-sensitive and non-viscous hydrogel bioinks. The book also presents sample applications in the areas of stem cells and cell matrix interaction. The book highlights pioneering results in the development of bioprinting technologies and bioinks, which were published in high-quality journals such as Advanced Materials, Biofabrication and ACS Biomaterials Science & Engineering. These include an in-situ crosslinking strategy that overcomes the viscosity limits for bioinks, which is virtually impossible using conventional strategies, and can be generalized for other bioink formulations.
Download or read book Development Characterization and Evaluation of a Photocrosslinkable Methacryloyl modified Decellularized ECM Bioink for 3D Bio Printing Applications written by Julián Andrés Serna Méndez and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Decellularized extracellular matrices are considered the next generation of biomaterials for the in vitro development of relevant tissues and organ-like constructs. Despite their high biocompatibility, resulting from their heterogeneous composition rich on structural proteins and growth factors, novel strategies for improving their mechanical properties before and after extrusion are still necessary for their direct bioprinting. Here we report photocrosslinkable hydrogels based on the methacryloyl modification of extracellular matrix (ECM) derived from decellularized small intestinal submucosa (SIS). The developed hydrogels feature shear-thinning behavior, printability for extrusion-based 3D bioprinting and thermosensitivity, as they form a gel at 37°C. Furthermore, rheological time sweep experiments showed that mechanical stiffness of the hydrogels is dependent on the energy dosage given to the material via exposition to blue light. This provides the possibility of tuning the rigidity of bioprinted constructs on will, depending mainly of the mechanical properties of the tissue to mimic. Primary amine quantification before and after biochemical modification of the hydrogels showed a reaction efficiency around 14 % and XPS analyses suggested the need for extending dialysis time after the functionalization. Bioprinting experiments provided a proof-of-concept that the bioinks can be directly bioprinted with high shape fidelity with no need of support scaffolds or baths, while maintaining cell viability above 70 %. Further experiments should be directed to characterize the biochemical composition of the materials in order to direct the developed bioinks toward the biofabrication of a specific tissue type.
Download or read book 3D Bioprinting written by Dong-Woo Cho and published by Springer Nature. This book was released on 2019-12-04 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.
Download or read book Designing Bioinks for 3D Bioprinting in Gel based Support Baths written by Christopher Dean Lindsay and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D bioprinting has emerged as a powerful tool to pattern extracellular matrix (ECM) and living cells to produce biological structures that mimic native tissues and organs ex vivo. Bioinks, the cell- and ECM-laden mixtures used during bioprinting, have primarily been composed of biopolymers appropriated from traditional 3D tissue engineering to form hydrogels following printing. These materials are not particularly well suited to meet the challenges introduced through bioprinting, namely, cell sedimentation, mechanical damage while printing, printing in gel-based support baths, and tunability of the mechanics and bioactivity of the biopolymer hydrogels. New bioink materials can be produced through intentional design of the crosslinking scheme of the hydrogel network before and after bioprinting into a gel-based support bath. The work presented in this thesis explores the benefits of designing new bioink materials for 3D bioprinting that move beyond the simplest crosslinking strategies used in traditional tissue engineering materials. Specifically, bioinks that employ two different stages of crosslinking, a first-stage crosslinking before the material is printed and a second stage following printing in a bath, prove to be effective for 3D bioprinting. One example of the increased versatility of bioinks from a dual-crosslinking approach is a bioprinted stem cell expansion lattice for stem cell manufacturing made using a traditional bioink material, sodium alginate. The second half of this thesis introduces two new crosslinking schemes that are made possible only by bioprinting into a gel-based support bath. Both bioorthogonal and hydrazone dynamic covalent chemistries are explored for producing new bioinks. The work put forward in this thesis is a step toward designing new bioinks for 3D bioprinting. An array of crosslinking schemes for bioinks are presented alongside creative uses of existing biopolymers to produce more adaptable bioinks.
Download or read book Cell Assembly with 3D Bioprinting written by Yong He and published by John Wiley & Sons. This book was released on 2022-03-14 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides an up-to-date outline of cell assembly methods and applications of 3D bioprinting Cell Assembly with 3D Bioprinting provides an accesible overview of the layer-by-layer manufacturing of living structures using biomaterials. Focusing on technical implemention in medical and bioengineering applications, this practical guide summarize each key aspect of the 3D bioprinting process. Contributions from a team of leading researchers describe bioink preparation, printing method selection, experimental protocols, integration with specific applications, and more. Detailed, highly illustrated chapters cover different bioprinting approaches and their applications, including coaxial bioprinting, digital light projection, direct ink writing, liquid support bath-assisted 3D printing, and microgel-, microfiber-, and microfluidics-based biofabrication. The book includes practical examples of 3D bioprinting, a protocol for typical 3D bioprinting, and relevant experimental data drawn from recent research. * Highlights the interdisciplinary nature of 3D bioprinting and its applications in biology, medicine, and pharmaceutical science * Summarizes a variety of commonly used 3D bioprinting methods * Describes the design and preparation of various types of bioinks * Discusses applications of 3D bioprinting such as organ development, toxicological research, clinical transplantation, and tissue repair Covering a wide range of topics, Cell Assembly with 3D Bioprinting is essential reading for advanced students, academic researchers, and industry professionals in fields including biomedicine, tissue engineering, bioengineering, drug development, pharmacology, bioglogical screening, and mechanical engineering.
Download or read book Fetal Porcine Membranes for the Development of Photocrosslinkable Bioinks with Application in Extrusion based 3d Bioprinting written by Paula Andrea Guerrero Barragán and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D bioprinting is an emerging technology that combines cells and matrix material to generate biomimetic structures with applications in regenerative medicine and drug testing models. Bioinks as hydrogels are an essential part of this technology and there are divided into synthetic and natural bioinks. Decellularized extracellular matrix (dECM) bioinks are in the natural category and are capable of providing cells an environment to properly function. The main advantage of dECM bioinks is the presence of structural and functional molecules from the native ECM tissue. For this reason, a bioink of dECM represents an attractive approach to bioprint cells and tissues. In this work, we present the decellularization process of amniotic porcine membrane (PAM) and the fabrication of a bioprinting PAM hydrogel to use as a bioink. As a result of those processes, we obtained an effectively decellularized PAM hydrogel, which is also responsive to temperature and light due to the conjugation of methacrylic acid and the presence of riboflavin as a photocrosslinker. The dPAM bioink characterization shows a pseudoplastic behavior that exhibits an increase in storage modulus in response to blue visible light and temperature. Also, upon photocrosslinking, the obtained material shows a significant decline in surface porosity when compared with temperature-induced gelation. Additionally, we demonstrated the printability and biocompatibility of our PAM bioink prior and after the bioprinting process.
Download or read book 3D Printing with Light written by Pu Xiao and published by Walter de Gruyter GmbH & Co KG. This book was released on 2021-04-06 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces fundamentals of 3D printing with light, photoinitiating system for 3D printing as well as resins. Plenty of applications, trends and prospects are also discussed, which make the book an essential reference for both scientists and industrial engineers in the research fields of photochemistry, polymer chemistry, rapid prototyping and photopolymerization.
Download or read book Essentials of 3D Biofabrication and Translation written by Anthony Atala and published by Academic Press. This book was released on 2015-07-17 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essentials of 3D Biofabrication and Translation discusses the techniques that are making bioprinting a viable alternative in regenerative medicine. The book runs the gamut of topics related to the subject, including hydrogels and polymers, nanotechnology, toxicity testing, and drug screening platforms, also introducing current applications in the cardiac, skeletal, and nervous systems, and organ construction. Leaders in clinical medicine and translational science provide a global perspective of the transformative nature of this field, including the use of cells, biomaterials, and macromolecules to create basic building blocks of tissues and organs, all of which are driving the field of biofabrication to transform regenerative medicine. Provides a new and versatile method to fabricating living tissue Discusses future applications for 3D bioprinting technologies, including use in the cardiac, skeletal, and nervous systems, and organ construction Describes current approaches and future challenges for translational science Runs the gamut of topics related to the subject, from hydrogels and polymers to nanotechnology, toxicity testing, and drug screening platforms
Download or read book Injectable Hydrogels for 3D Bioprinting written by Insup Noh and published by Royal Society of Chemistry. This book was released on 2021-07-23 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hydrogels represent one of the cornerstones in tissue engineering and regenerative medicine, due to their biocompatibility and physiologically relevant properties. These inherent characteristics mean that they can be widely exploited as bioinks in 3D bioprinting for tissue engineering applications as well as injectable gels for cell therapy and drug delivery purposes. The research in these fields is booming and this book provides the reader with a terrific introduction to the burgeoning field of injectable hydrogel design, bioprinting and tissue engineering. Edited by three leaders in the field, users of this book will learn about different classes of hydrogels, properties and synthesis strategies to produce bioinks. A section devoted to the key processing and design challenges at the hydrogel/3D bioprinting/tissue interface is also covered. The final section of the book closes with pertinent clinical applications. Tightly edited, the reader will find this book to be a coherent resource to learn from. It will appeal to those working across biomaterials science, chemical and biomedical engineering, tissue engineering and regenerative medicine.
Download or read book 3D Bioprinting of Self Standing Silk Based Bioink written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bone Tissue Engineering written by Jeffrey O. Hollinger and published by CRC Press. This book was released on 2004-10-14 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on bone biology, Bone Tissue Engineering integrates basic sciences with tissue engineering. It includes contributions from world-renowned researchers and clinicians who discuss key topics such as different models and approaches to bone tissue engineering, as well as exciting clinical applications for patients. Divided into four sections, t
Download or read book Bioprinting in Regenerative Medicine written by Kursad Turksen and published by Springer. This book was released on 2015-09-02 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the current state of laser-assisted bioprinting, a cutting edge tissue engineering technology. Nineteen chapters discuss the most recent developments in using this technology for engineering different types of tissue. Beginning with an overview, the discussion covers bioprinting in cell viability and pattern viability, tissue microfabrication to study cell proliferation, microenvironment for controlling stem cell fate, cell differentiation, zigzag cellular tubes, cartilage tissue engineering, osteogenesis, vessel substitutes, skin tissue and much more. Because bioprinting is on its way to becoming a dominant technology in tissue-engineering, Bioprinting in Regenerative Medicine is essential reading for those researching or working in regenerative medicine, tissue engineering or translational research. Those studying or working with stem cells who are interested in the development of the field will also find the information invaluable.
Download or read book Crosslinking Strategies for 3D Bioprinting of Engineered Hydrogels written by Sarah Mei Hull and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: 3D bioprinting has demonstrated potential for spatially patterning cells and materials together to create structures that recapitulate native tissue. By mimicking these structural features, bioprinted constructs can serve as in vitro models for basic research or can be implanted into the body for regenerative medicine applications. However, this technique is limited by a lack of suitable soft materials for use as bioinks since it remains challenging to design materials that are both printable and can support cell culture. In addition, as matrix material properties are known to influence cell phenotype and behavior, it is becoming increasingly important to tailor the bioink's properties to each individual cell type in order to create more complex, functional printed structures. In this thesis, I explore how engineered, tunable hydrogels can be used to address the current limitations of 3D bioprinting and then develop crosslinking strategies to control the mechanical, biochemical, and dynamic behavior of bioink materials before and after printing.
Download or read book Emerging Technologies In Biophysical Sciences A World Scientific Reference In 3 Volumes written by and published by World Scientific. This book was released on 2022-10-14 with total page 1078 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 1:Biofabrication aims to produce artificially manufactured tissues and organs, potentially revolutionizing conventional paradigm of clinical practice in treating diseases and extending the life span and quality of human beings. In this volume, we invite notable experts in the field of biofabrication and biomanufacturing to summarize recent rapid progress in this field from multifaceted aspects covering biofabrication techniques and building materials such as scaffold and living cells. Specifically, a focus is placed on a variety of techniques derived from 3D bioprinting and bioassembly strategies, such as acoustic assembly and electrofabrication. Moreover, principles and strategies for choosing hydrogels and polymers for biofabrication are also heavily discussed. Overall, this book creates a good opportunity for undergraduate and postgraduate students as well as bioengineers and medical researchers who wish to gain a fundamental understanding of current status and future trends in biofabrication and biomanufacturing.Volume 2:Infertility has become a significant psychosocial burden affecting the lives of couples who cannot reproduce naturally. Advanced reproductive technologies (ARTs) are being developed to treat infertility. This handbook explores significant development of ARTs for fertility testing, selection of sperm, oocyte and embryo, reproductive monitors, automation in embryology, and fertility preservation. This volume provides a comprehensive overview of the myriad of emerging technologies and systems that are being utilized or will be utilized in near future in reproductive clinics. Overall this book creates a good opportunity for undergraduate and postgraduate students as well as scientists and medical researchers who wish to gain fundamental understanding of current status and future trends in fertility and reproductive medicine.Volume 3:Healthcare industry has a notable paradigm transition from centralized care to the point-of-care (POC). During this metamorphosis, a number of new technologies and strategies have been adapted to the current practice, addressing the existing challenges in the fields of medicine and biology. All the efforts aim to improve the clinical management and the effectiveness and quality of care. In particular, diagnostics has pivotal roles in guiding clinical management for the most effective treatment to control and cure the disease. In contrast to the existing diagnostic strategies employing bulky-sized tools, expensive infrastructure, laborious protocols, and lengthy processing steps, the contribution of biosensors to current healthcare system, especially to diagnostics, is paramount. The unprecedented and admirable characteristics of biosensing strategies have expanded our knowledge on medicine and biology by harmonizing materials science, chemistry, physics, and engineering. We believe that biosensors applied to disease diagnostics will not only garner more attention in clinical research to decipher disease biology and mechanism, and also, stimulate innovative perspectives in artificial intelligence (AI) and internet of things (IoT) synergistically, thereby their more facile adaptation to daily-use. Overall this book creates a good opportunity for undergraduate and postgraduate students as well as scientists and medical researchers who wish to gain fundamental understanding of current status and future trends in diagnostic technologies.
Download or read book 3D Printing and Biofabrication written by Aleksandr Ovsianikov and published by Springer. This book was released on 2018-06-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an in-depth introduction to 3D printing and biofabrication and covers the recent advances in additive manufacturing for tissue engineering. The book is divided into two parts, the first part on 3D printing discusses conventional approaches in additive manufacturing aimed at fabrication of structures, which are seeded with cells in a subsequent step. The second part on biofabrication presents processes which integrate living cells into the fabrication process.
Download or read book A Handheld 3D Bioprinter for in Situ Tissue Regeneration written by Anton Hjaltason and published by . This book was released on 2024 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the field of tissue engineering, three-dimensional (3D) bioprinting has emerged as a novel approach to make hydrogel scaffolds in situ with a variety of shapes and sizes. This method involves the extrusion of cells and biomaterials in filaments, enabling layer-by-layer scaffold deposition, followed by photo-mediated crosslinking. Its versatility makes it uniquely suited for personalized tissue regeneration, where patient-specific hydrogel scaffolds may be applied to wound areas with irregular shapes. One caveat of current 3D bioprinters on the market is that they are typically desktop systems. While these devices can fabricate many scaffold shapes with high fidelity, it is difficult for the computer system to exactly match the complex geometries seen in many wounds. Handheld 3D bioprinters can resolve this issue by directly depositing bioinks onto an area of the body. Handheld bioprinters currently exist on the market and in the laboratory, but many are unergonomic, require bulky external equipment, or lack specific flow rate control. This project involves the design and testing of a new handheld 3D bioprinter that can print a gelatin methacryloyl (GelMA)-based granular bioink in situ. Granular bioinks have been developed to solve the challenge of nanoporous bulk hydrogels by taking advantage of the void space among microscale hydrogel particles (microgels). These microgels can be mixed with nanoparticles and a photoinitiator to make a nanoengineered granular bioink, NGB, which is then 3D bioprinted into a scaffold. The design of the handheld bioprinter developed in this project includes a mechanical piston extrusion system, specific flow rate control, and a small form factor. The goal of these experiments is to combine the technology of handheld 3D bioprinting with NGB to bring this new bioink into surgical settings in the future. The results indicate that the handheld bioprinter is simple and inexpensive to manufacture. Additionally, it can print NGB somewhat effectively and shows promise for future experiments with the granular bioink.
