Download or read book A Molecular Genetic Analysis of Axon Guidance in Drosophila Melanogaster written by Huidy Shu and published by . This book was released on 2001 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Molecular Genetic Analysis of Motoneuron Axon Guidance in Drosophila Melanogaster written by Gordon Clark Teg Pipes and published by . This book was released on 2000 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Molecular Genetic Analysis of Target Selection in Drosophila Melanogaster written by Roger Chi-Che Lee and published by . This book was released on 2002 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Molecular Genetic Analysis of the Role of the Guanine Nucleotide Exchange Factor Trio During Axon Pathfinding in the Embryonic CNS of Drosophila Melanogaster written by David J. Forsthoefel and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The Drosophila melanogaster embryo is an ideal system in which to study axon guidance, because of the relative simplicity of the nervous system and the evolutionary conservation of the molecules utilized during development. The Abelson cytoplasmic tyrosine kinase regulates actin cytoskeletal dynamics in Drosophila, mice, and humans. In Drosophila, Abl is expressed in the developing central and peripheral nervous systems (CNS and PNS). In a genetic screen for modifiers of the Abl mutant semilethality phenotype, we identified trio, a cytoplasmic guanine nucleotide exchange factor that is also expressed in the CNS and regulates actin dynamics through Rho GTPases. Mutations in Abl and trio interacted genetically, leading to dramatic disruption of axon pathways at the CNS midline. Building upon these initial observations, we analyzed interactions between Abl, trio, and the attractive Netrin receptor frazzled (fra)/Deleted-in-Colorectal-Cancer (DCC). In fra;Abl and fra;trio double mutants, few axons crossed the midline, similar to the phenotype in trio, Abl mutants. Furthermore, mutations in Abl and trio suppressed the inappropriate midline crossover phenotype in embryos expressing the chimeric Robo-Fra receptor, consistent with an in vivo role for these molecules as Fra effectors. Fra bound Abl and Trio in coimmunoprecipitation and GST pulldown experiments, and tyrosine phosphorylation of Fra and Trio was elevated in cultured cells overexpressing Abl. Mutations in enabled (ena), another Abl substrate, suppressed the loss-of-commissure phenotype in fra, Abl, and trio mutants, as well as the Robo-Fra receptor phenotype. Together, these results suggest that Abl and Trio are effectors for multiple attractive receptors at the CNS midline, and that Ena may function during both attractive and repulsive signaling. Finally, a functional analysis of the requirement for Trio's conserved domains has been initiated. In transgenic rescue and overexpression experiments, TrioGEF1 was required for axon guidance across the CNS midline, while TrioSH3 inhibited midline crossing. Coexpression experiments with the Robo-Fra receptor and assays in other tissues and cultured cells suggest that the conserved N-terminal domain, spectrin-like repeats, and GEF2 domain may modulate GEF1 signaling in specific contexts. Future experiments must elucidate the mechanistic details of cytoskeletal control by Trio and Fra.

Download or read book Genetic Analysis of Axon Guidance in Drosophila Melanogaster written by Ashley Palani Wright and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Molecular and Genetic Analysis of Neuromuscular Connectivity and Synaptic Growth in Drosophila Melanogaster written by Hong Iris Wan and published by . This book was released on 2000 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Genetics of Axon Guidance in Drosophila Melanogaster written by Qi Sun and published by . This book was released on 2000 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Genetic and Molecular Analysis of Neuromuscular Connectivity in Drosophila Melanogaster written by Douglas McIntosh Fambrough and published by . This book was released on 1996 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Analysis of Signaling Pathways Involved in Drosophila Embryonic Axon Guidance written by Jessica Elizabeth Palmer and published by . This book was released on 2003 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular and Genetic Analysis of Position Effect Variegation in Drosophila Melanogaster written by Patrick Morcillo and published by . This book was released on 1993 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of the Role of the Plexin Receptor and the Rho Family GTPase Regulators in Axon Guidance in the Embryonic Central Nervous System of Drosophila Melanogaster written by Hailan Hu and published by . This book was released on 2002 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Analysis of the Maintenance of Neuronal Morphology in Drosophila Melanogaster written by Jessica LaMae Whited and published by . This book was released on 2006 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: (Cont.) Establishment and maintenance of complex axonal trajectories is also a key feature of neuronal mophology. I identified a requirement for a novel cytoplasmic tyrosine phosphatase, PTPMEG, in these processes. Normal mushroom bodies, structures critical for insect learning and memory, have dorsally-projecting alpha lobe and medially-projecting beta lobe axons. Alpha lobes develop normally in ptpmeg mutants, but their pattern is not maintained. Instead, alpha lobe axons retract during pupation, resulting in thin and/or shortened alpha lobes. Meanwhile, beta lobe axons overextend at the midline. Removing ptpmeg function in mushroom bodies does not cause mutant phenotypes. ptpmeg mutants are rescued by pan-neuronal expression of wild-type Ptpmeg, but not by versions with disrupted phosphatase activity. These data suggest that Ptpmeg activity is required in another type of neuron to prevent mushroom body axon retraction. Ellipsoid body axons normally form a ring structure in the central brain. In ptpmeg mutants, the ellipsoid body axons develop abnormally, with the ventral side of the ring being discontinuous; the defect can be rescued by expression of wild-type Ptpmeg pan-neuronally.

Download or read book A Phenotypic Genetic and Molecular Analysis of the Developing Drosophila Melanogaster Visual System written by Benjamin N. R. Cheyette and published by . This book was released on 1994 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Screen for New Motor Axon Guidance Mutants written by Christopher Aaron Korey and published by . This book was released on 2001 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Drosophila melanogaster Practical Uses in Cell and Molecular Biology written by and published by Academic Press. This book was released on 1995-01-25 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drosophila melanogaster: Practical Uses in Cell and Molecular Biology is a compendium of mostly short technical chapters designed to provide state-of-the art methods to the broad community of cell biologists, and to put molecular and cell biological studies of flies into perspective. The book makes the baroque aspects of genetic nomenclature and procedure accessible to cell biologists. It also contains a wealth of technical information for beginning or advanced Drosophila workers. Chapters, written within a year of publication, make this topical volume a valuable laboratory guide today and an excellent general reference for the future.Key Features* Collection of ready-to-use, state-of-the art methods for modern cell biological and related research using Drosophila melanogaster* Accessible to both experienced Drosophila researchers and to others who wish to join in at the cutting edge of this system * Drosophila offers an easily managed life cycle, inexpensive lifestyle, extraordinarily manipulable molecular and classical genetics, now combined with powerful new cell biology techniques * Introduction and overview sections orient the user to the Drosophila literature and lore * Six full-color plates and over 100 figures and tables enhance the understanding of these cell biology techniques

Download or read book A Phenotypic and Molecular Genetic Analysis of the Furry Gene in Drosophila Melanogaster written by Jingli Cong and published by . This book was released on 2001 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structure function Analysis of the Axon Guidance Molecules Sidestep and Beaten Path Ia in Drosophila Melanogaster written by Caroline Daniela Stefanie Heymann and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: