Download or read book Western Blotting Guru written by Ayaz Najafov and published by Academic Press. This book was released on 2017-07-07 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types. Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments Contains unique good practices and tips that are indispensable for the beginner and expert alike Features special cases with applications of immunoblotting optimization Includes detailed appendices with tables, figures and key protocols Provides troubleshooting tips for various types of modifications of standard protocols Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem
Download or read book Western Blotting for the Non Expert written by Biji T. Kurien and published by Springer Nature. This book was released on 2021-07-24 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..
Download or read book Western Blotting written by Biji T. Kurien and published by . This book was released on 2015 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Western Blot Simple Guide and Important Tips for a Beginner written by Che Aishah Nazariah Ismail, Mahaneem Mohamed, Victor Udo Nna and published by Penerbit USM. This book was released on 2023-10-11 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first time you know that you need to conduct Western blotting, you may be nervous since there are many critical steps to follow. It would be advantageous to the experienced researcher, but for the first-timer, especially postgraduate students, it can be such a hassle! This book will guide the researcher who is new to Western blotting, step by step from the scratch, in a simple and illustrative way. This book benefits the reader as it provides a simple illustrative guide starting from protein extraction to visualization of protein bands with some troubleshooting guides to improve the protein bands quality.
Download or read book Protein Blotting and Detection written by Biji T. Kurien and published by . This book was released on 2009-01-01 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
Download or read book Self study Course 3031 G written by and published by . This book was released on 1990 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Blotted Proteins written by Biji T. Kurien and published by Humana. This book was released on 2016-10-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.
Download or read book Protein Blotting written by Bonnie S. Dunbar and published by Oxford University Press, USA. This book was released on 1994 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide for professionals and students in the life sciences to a laboratory technique most commonly used to detect protein antigens, but recently also being used to characterize a wide range of different interactions between proteins and other proteins, carbohydrates, and DNA. Describes the equipment, principles, sample preparation, detection methods, and several conventional and innovative applications. Annotation copyright by Book News, Inc., Portland, OR
Download or read book Western Blot Marker with Universal Immunoglobulin Binding Domain written by Andrew Fleischman and published by . This book was released on 2010 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunological techniques such as western blotting harness the specificity and avidity of antibodies to detect proteins. In western blotting, antibodies specifically detect proteins separated by SDS-PAGE electrophoresis. Molecular weight markers are important components of a western blot because they allow one to verify protein sizes. However, proteins in a traditional molecular weight marker will not appear on the film of a western blot, since the various primary antibodies cannot recognize them. The goal of this thesis research is to develop universal western blot molecular weight markers that bind commonly used secondary antibodies. The IgG Binding Domain of Protein A (PRA), a domain that binds the heavy chain region of antibodies, was fused to proteins of various sizes. Each recombinant construct was expressed and purified on a small scale to determine the optimal expression conditions and to check the solubility of the protein. These optimal conditions were then used to express the protein on a larger scale. Each marker protein was purified by metal affinity chromatography via the N-terminal polyhistidine tag. The purified proteins were combined into a mixture, producing a set of western blotting molecular weight markers that be used independent of the primary antibody employed.
Download or read book Western Blotting Handbook and Troubleshooting Guide written by Thermo Fisher Scientific (Firm) and published by . This book was released on 2009 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Overview of Blotting Techniques written by Shiv Sanjeevi Sripathi and published by Delve Publishing. This book was released on 2020-11 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: There are 2 techniques that are commonly employed in research labs: gel electrophoresis and blotting. Following electrophoresis, separated nucleic acids and proteins can be transferred to a membrane where they are analyzed or detected. Depending on the molecule being analyzed, Southern blot refers to transfer and studying DNA, Northern blotting refers to RNA, Western blotting to proteins and eastern blotting for post-translational modifications. Given the importance of blotting in the lab, this book comes at an opportune time. The book discusses the protocols and applications of blotting-Southern, western, northern and eastern blotting. Case studies have also presented to help the reader gain insights into the practicality and applicability of blotting in today's world. The editors hope that this book will serve as a lab staple just like the aforementioned blotting techniques.
Download or read book Molecular Genetics of Cancer written by John K. Cowell and published by Gulf Professional Publishing. This book was released on 2001 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first volume was published, there has been significant success in isolating genes responsible for particular cancers as well as a major improvement in our understanding of the molecular events leading to tumors. This book explores possible genetic treatments that can suppress cancer cells that have formed tumors and it presents the details of the isolation and characterization of new human cancer genes that have recently been identified. Molecular Genetics of Cancer, 2E is an essential book for anyone involved in cancer research and the search for a cure.
Download or read book Molecular Genetics of Cancer written by John Cowell and published by Garland Science. This book was released on 2003-12-16 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Genetics of Cancer, Second Edition provides an authoritative and up to date review of the key genes known to be critical in the development or progression of cancer. Throughout the book, scientific advances and their clinical relevance are covered in detail, particularly in the light of findings concerning the inheritance of genes predisposing to tumorigenesis. The book is therefore a valuable source of reference for clinicians and genetic counsellors as well as researchers.
Download or read book Olivia Joules and the Overactive Imagination written by Helen Fielding and published by Penguin. This book was released on 2005-04-26 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the close of the last millennium, Helen Fielding debuted the irrepressible (and blockbuster-bestselling) Bridget Jones. Now, Fielding gives us a sensational new heroine for a new era...Move over 007, a stunning, sexy-and decidedly female-new player has entered the world of international espionage. Her name is Olivia Joules (that’s "J.O.U.L.E.S. the unit of kinetic energy") and she's ready to take America by storm with charm, style, and her infamous Overactive Imagination. How could a girl not be drawn to the alluring, powerful Pierre Ferramo-he of the hooded eyes, impeccable taste, unimaginable wealth, exotic international homes, and dubious French accent? Could Ferramo really be a major terrorist bent on the Western world’s destruction, hiding behind a smokescreen of fine wines, yachts, and actresses slash models? Or is it all just a product of Olivia Joules’s overactive imagination? Join Olivia in her heart-stopping, hilarious, nerve-frazzling quest from hip hotel to eco-lodge to underwater cave, by light aircraft, speedboat, helicopter, and horse, in this witty, contemporary, and utterly unputdownable novel deluxe.
Download or read book Protein Blotting written by B. A. Baldo and published by S Karger Ag. This book was released on 1989 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Western Blotting Technical Manual written by Amersham Corporation and published by . This book was released on 199? with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Blotting written by and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
