Download or read book Use of Expression Cloning to Identify Novel Antigens Recognized on Human Prostate Tumors to Act as Targets of Cellular Immunotherapy written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our aim is to identify epitopes on the surface of prostate cells that are largely or exclusively tissue- or tumor- specific. This is done by expansion of T cells reactive for HLA-restricted, established tumor lines from the peripheral blood cells and tumor-infiltrating lymphocytes of cancer patients. Towards this end, we established a reagent bank of patient material that includes tissue, cells, and serum. We successfully and stably gene-modified the established prostate cell line, LNCaP, so that it expresses HLA-A2 and HLA-Al. We successfully transfected two other cell lines with these genes, transiently but sufficiently, for our in vitro stimulation technique. Several potentially HLA-restricted, tumor-specific T cells have been identified and are currently being cloned for retesting. In order to study tissue distribution, primary normal and tumor prostate tissue are being cultured by methods developed in our laboratory. In addition, we are performing an antibody-based method of antigen discovery that utilizes patient serum. Using this approach, several genes have been identified some of which represent previously unreported, novel genes. Studies are underway to discern the exact epitope being recognized. Studies are also underway to assess the level of gene expression in normal vs. tumor tissue from representative regions of the body.
Download or read book Biotechnology Entrepreneurship written by Michael L. Salgaller and published by . This book was released on 2010 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology Entrepreneurship: From Science to Solutions fills a critical gap in the biotechnology industry. For all the resources on how to start companies and on how to manage established companies in other sectors, there is a dearth of material on unique and critical issues in starting biotechnology companies, as well as managing the transition from start-up to established company. It is to this gap that Biotechnology Entrepreneurship is directed. By combining the voices of a diverse set of industry insiders with extensive experience in biotechnology, Biotechnology Entrepreneurship prepares nascent founders, managers, investors, and other biotechnology company stakeholders to position themselves and their companies for commercial success.
Download or read book Vaccines for Cancer Immunotherapy written by Nima Rezaei and published by Academic Press. This book was released on 2018-10-17 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Therapeutic cancer vaccines represent a type of active cancer immunotherapy. Clinicians, scientists, and researchers working on cancer treatment require evidence-based and up-to-date resources relating to therapeutic cancer vaccines. Vaccines for Cancer Immunotherapy provides a reference for cancer treatment for clinicians and presents a well-organized resource for determining high-potential research areas. The book considers that this promising modality can be made more feasible as a treatment for cancer. Chapters cover cancer immunology, general approaches to cancer immunotherapy, vaccines, tumor antigens, the strategy of allogeneic and autologous cancer vaccines, personalized vaccines, whole-tumor antigen vaccines, protein and peptide vaccines, dendritic cell vaccines, genetic vaccines, candidate cancers for vaccination, obstacles to developing therapeutic cancer vaccines, combination therapy, future perspectives and concluding remarks on therapeutic cancer vaccines. - Introduces the feasible immunotherapeutic vaccines for patients with different types of cancer - Presents the status of past and current vaccines for cancer treatment - Considers advantages and disadvantages of different therapeutic cancer vaccines - Looks at the combination of vaccines and other modalities, including immunotherapeutic and conventional methods - Analyzes obstacles to development of therapeutic cancer vaccines - Gives a view on future perspectives in the application of therapeutic cancer vaccines
Download or read book Novel Technology for Cloning Prostate Cancer Cell Markers written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of the research is to clone, isolate and characterize protein markers that are specifically expressed by advanced stage, androgen-independent prostate cancers. Two types of markers with this specificity are expected to be identified: (1) proteins secreted specifically by advanced stage prostate cancer cells, potentially useful in serum-based assays designed to specifically detect this class of tumors; and (2) markers expressed specifically on the surface of these cells, potentially useful as specific targets for clinical treatment of androgen-independent prostate cancer. Progress to date has involved: (1) preparation of membrane fraction mRNA's from four human cell lines representing four stages of prostate cancer, and use of these mRNA's to prepare cDNA libraries; (2) isolation of proteins from membrane fractions of two of these cell lines, and initiation of a collaboration to employ the techniques of Proteomics to differentially analyze these protein populations. We can now apply the new DNA microarray technology to the cDNA libraries described in (1) to identify clones possessing the characteristics described above. In addition, we are now employing the proteins described in (2) above for differential screening via two-dimensional gel analysis, to directly identify cell surface proteins specifically expressed by advanced stage prostate cancer cells.
Download or read book Tumor Immunology and Immunotherapy written by Robert C. Rees and published by Oxford University Press, USA. This book was released on 2014 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tumor immunology and immunotherapy provides a comprehensive account of cancer immunity and immunotherapy. Examining recent results, current areas of interest and the specific issues that are affecting the research and development of vaccines, this book provides insight into how these problems may be overcome as viewed by leaders in the field.
Download or read book Novel Technology for Cloning Prostate Cancer Cell Merkers written by and published by . This book was released on 2001 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purpose of project is to clone, isolate and characterize protein markers specifically expressed by advanced stage, androgen-independent prostate cancers. Two types of markers with this specificity are targeted: proteins secreted specifically by advanced stage prostate cancer cells, potentially useful in serum-based assays for these tumors; markers expressed specifically on the surface of these cells, potentially useful as advanced prostate cancer. Work during the period covered included: (1) Incorporation of the powerful new DNA Microarray Technology into the project, and its use to investigate differential gene expression in the LNCaP series of human cell lines, representing different stages of prostate cancer. (2) Studies demonstrating potential utility but insufficient purity of membrane fraction (MF) prepared from these cells; and use of an alternative fractionation scheme, successful only with the most advanced cells (C4-2B) . We plan to complete the studies under (1), to identify genes regulated during prostate cancer progression; and to use cDNA to the C4-2B MF RNA to probe DNA microarrays, to identify genes
Download or read book Comparative Oncology written by Alecsandru Ioan Baba and published by . This book was released on 2007 with total page 787 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Therapy for Cancer written by Kelly K. Hunt and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: The three sections of this volume present currently available cancer gene therapy techniques. Part I describes the various aspects of gene delivery. In Part II, the contributors discuss strategies and targets for the treatment of cancer. Finally, in Part III, experts discuss the difficulties inherent in bringing gene therapy treatment for cancer to the clinic. This book will prove valuable as the volume of preclinical and clinical data continues to increase.
Download or read book Development of a Novel Methodology for Improving CTL Recognition of Prostate Specific Antigen PSA for the Immunotherapy of Prostate Cancer written by and published by . This book was released on 2001 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt: The major goal of the proposed research is to develop a novel methodology for improving T cell epitopes. The underlying hypothesis is that T cells capable of recognizing tumor- associated antigens (TAA) are present but often difficult to activate. However, once activated such T cells might be effective against tumors due to the less stringent triggering requirements of mature effectors. We proposed to develop a novel bacterial expression system for modifying and screening the epitopes of PSA, a known TAA. In the current year, we have employed a saturation mutagenesis technique to a PSA peptide epitope we have identified. Expression libraries were constructed corresponding to each position in the peptide. These libraries were screened functionally, The clones showing enhanced activity were sequenced. From these screens, we were able to identify a number of enhanced peptide epitopes. Peptides were synthesized and tested in a functional assay. These peptides showed enhanced activity with the T cell hybridoma. The findings show that altered peptide ligands can be discovered using the novel methodology proposed. These approaches should be applicable to other tumor antigens.
Download or read book Chimeric Antigen Receptor T Cell Therapies for Cancer E Book written by Daniel W. Lee and published by Elsevier Health Sciences. This book was released on 2019-11-30 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: From patient referral to post-therapy management, Chimeric Antigen Receptor (CAR) T-Cell Therapies for Cancer: A Practical Guide presents a comprehensive view of CAR modified T-cells in a concise and practical format. Providing authoritative guidance on the implementation and management of CAR T-cell therapy from Drs. Daniel W. Lee and Nirali N. Shah, this clinical resource keeps you up to date on the latest developments in this rapidly evolving area. - Covers all clinical aspects, including patient referral, toxicities management, comorbidities, bridging therapy, post-CAR monitoring, and multidisciplinary approaches to supportive care. - Includes key topics on associated toxicities such as predictive biomarkers, infections, and multidisciplinary approaches to supportive care. - Presents current knowledge on FDA approved CAR T-cell products as well as developments on the horizon. - Editors and authors represent leading investigators in academia and worldwide pioneers of CAR therapy.
Download or read book Immune Memory and Vaccines Great Debates written by Shane Crotty and published by Perspectives Cshl. This book was released on 2018-01-31 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: "A subject collection from the Cold Spring Harbor perspectives in biology."
Download or read book Heat Shock Proteins in Cancer written by Stuart K. Calderwood and published by Springer Science & Business Media. This book was released on 2007-09-09 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heat shock proteins are emerging as important molecules in the development of cancer and as key targets in cancer therapy. These proteins enhance the growth of cancer cells and protect tumors from treatments such as drugs or surgery. However, new drugs have recently been developed particularly those targeting heat shock protein 90. As heat shock protein 90 functions to stabilize many of the oncogenes and growth promoting proteins in cancer cells, such drugs have broad specificity in many types of cancer cell and offer the possibility of evading the development of resistance through point mutation or use of compensatory pathways. Heat shock proteins have a further property that makes them tempting targets in cancer immunotherapy. These proteins have the ability to induce an inflammatory response when released in tumors and to carry tumor antigens to antigen presenting cells. They have thus become important components of anticancer vaccines. Overall, heat shock proteins are important new targets in molecular cancer therapy and can be approached in a number of contrasting approaches to therapy.
Download or read book Translational Immunotherapy of Brain Tumors written by John H. Sampson and published by Academic Press. This book was released on 2017-02-06 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Translational Immunotherapy of Brain Tumors gives researchers and practitioners an up-to-date and comprehensive overview of the field. Chapters include adoptive immunotherapy, immunosuppression, CAR therapy of brain tumors, and dendritic cell therapy for brain tumors. Very few agents have been shown to be efficacious in the treatment of malignant gliomas. Recently, there have been a number of studies demonstrating the potential success of immunotherapy for brain tumors. Immunotherapeutics are becoming the most frequent drugs to be used in cancer therapy. These new breakthroughs, now approved by the FDA, are a part of multiple phase III international trials and ongoing research in malignant glioma, meaning that the information in this cutting-edge book will be of great importance to practitioners and researchers alike. Comprehensive overview, providing an update on immunology, translational immunotherapy, and clinical trials relating to malignant gliomas Edited by a prominent neurosurgeon with contributions by leading researchers in the field Ideal resource for researchers and practitioners interested in learning about mechanisms that use the immune system to treat brain tumors
Download or read book Novel Technologies for Vaccine Development written by Igor S Lukashevich and published by Springer. This book was released on 2014-11-13 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the development of new viral vector-based vaccines before discussing two major applications: preventive vaccines for infectious diseases and therapeutic cancer vaccines. Viral vector-based vaccines hold a great potential for development into successful pharmaceutical products and several examples at the advanced pre-clinical or clinical stage are presented. Nevertheless, the most efforts were focused on novel and very innovative technologies for new generation of vector-based vaccines. Furthermore, specific topics such as delivery and adjuvant and protection strategies for cell-mediated-based vaccines are presented. Given its scope, the book is a “must read” for all those involved in vaccine development, both in academia and industrial vaccine development.
Download or read book Medicinal Chemistry of Anticancer Drugs written by Carmen Avendaño and published by Elsevier. This book was released on 2015-06-11 with total page 767 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicinal Chemistry of Anticancer Drugs, Second Edition, provides an updated treatment from the point of view of medicinal chemistry and drug design, focusing on the mechanism of action of antitumor drugs from the molecular level, and on the relationship between chemical structure and chemical and biochemical reactivity of antitumor agents. Antitumor chemotherapy is a very active field of research, and a huge amount of information on the topic is generated every year. Cytotoxic chemotherapy is gradually being supplemented by a new generation of drugs that recognize specific targets on the surface or inside cancer cells, and resistance to antitumor drugs continues to be investigated. While these therapies are in their infancy, they hold promise of more effective therapies with fewer side effects. Although many books are available that deal with clinical aspects of cancer chemotherapy, this book provides a sorely needed update from the point of view of medicinal chemistry and drug design. - Presents information in a clear and concise way using a large number of figures - Historical background provides insights on how the process of drug discovery in the anticancer field has evolved - Extensive references to primary literature
Download or read book Immunopharmacology written by Manzoor M. Khan and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decades, with the introduction of the recombinant DNA, hybridoma and transgenic technologies there has been an exponential evolution in understanding the pathogenesis, diagnosis and treatment of a large number of human diseases. The technologies are evident with the development of cytokines and monoclonal antibodies as therapeutic agents and the techniques used in gene therapy. Immunopharmacology is that area of biomedical sciences where immunology, pharmacology and pathology overlap. It concerns the pharmacological approach to the immune response in physiological as well as pathological events. This goals and objectives of this textbook are to emphasize the developments in immunology and pharmacology as they relate to the modulation of immune response. The information includes the pharmacology of cytokines, monoclonal antibodies, mechanism of action of immune-suppressive agents and their relevance in tissue transplantation, therapeutic strategies for the treatment of AIDS and the techniques employed in gene therapy. The book is intended for health care professional students and graduate students in pharmacology and immunology.
Download or read book Progress in Cancer Immunotherapy written by Shuren Zhang and published by Springer. This book was released on 2016-05-30 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides readers an extensive overview of recent progress in basic and clinical research on cancer immunotherapy. Thanks to rapid advances in molecular biology and immunology, it has become increasingly evident that cancer growth is influenced by host immune responses. With the success of a number of clinical trials, immunotherapy has become a promising treatment modality of cancer. This book covers five major topics, including monoclonal antibodies, biological response modifiers, cancer vaccines, adoptive cellular therapy and oncolytic viruses. It also examines the combination of different immune strategies as well as the combination of immunotherapy with other treatments to increase anti-tumor effects. Through the comprehensive discussion of the topic, the book sheds valuable new light on the treatment of tumors.
