Download or read book Transcriptional Regulation of the G1 S Cell Cycle Transition in Saccharomyces Cerevisiae written by Herman Wijnen and published by . This book was released on 2000 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by Michael Ross Harris and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by and published by . This book was released on 2014 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Connections Between Growth and Division written by Laura Lynn Newcomb and published by . This book was released on 2001 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spatio Temporal Regulation of Cell Cycle Transcription written by Umut Eser and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Start checkpoint regulates cell cycle commitment and associated transcription in the budding yeast, Saccharomyces cerevisiae. It was previously shown that commitment to cell division corresponds to activating the positive feedback loop of G1 cyclins controlled by the transcription factors SBF and MBF. Around this pivotal cell cycle event, over 300 genes (G1/S regulon) are expressed to facilitate the G1/S transition. Despite its importance, little was known about distinct temporal regulation within the G1/S regulon. We found that SBF and MBF target genes have a well-defined distribution of transcriptional activation times. We also showed that activation of G1 cyclins precedes the activation of the bulk of the G1/S regulon, which we named 'feedback-first' regulation. In budding yeast, feedback-first regulation ensures that commitment to cell division occurs before large-scale changes in transcription. Thus, the transition can be viewed as a two-step process whereby the decision to divide precedes synthesis of the cellular machinery required for division. Furthermore, we found that feedback-first regulation is conserved in the related yeast S. bayanus as well as human cells. This finding highlighted the importance of understanding the molecular mechanisms through which co-regulated genes can have distinct activation dynamics. We showed that timing is partially explained by the combinatorial use of SBF and MBF transcription factors, which implement a logical OR function for gene activation. In addition to combinatorial use of transcription factors, we analyzed genome-wide chromosome conformation capture data to examine the potential link between the timing of gene expression and 3-D genome architecture. The early-activated genes of the G1/S regulon are significantly enriched for the number of physical contacts to the rest of the genome. Further analysis revealed two main clusters, whose interactions co-vary and whose activation time distributions are distinct. Taken together, these our work explains a significant amount of timing variation within cell cycle-dependent gene expression. Thus, we concluded that the cell utilizes both genome architecture and the combinatorial use of transcription factors to implement feedback-first regulation ensuring that commitment to cell division precedes genome-wide cell cycle-dependent transcription.
Download or read book Transcriptional Regulation During the GI to S Transition of the Yeast Cell Cycle written by Yan Zhu and published by . This book was released on 1996 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation During the G1 to S Transition of the Yeast Cell Cycle written by Yan Zhu and published by . This book was released on 1996 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Cycle Regulation written by Philipp Kaldis and published by Results and Problems in Cell Differentiation. This book was released on 2006-06-26 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a state-of-the-art summary of the latest achievements in cell cycle control research with an outlook on the effect of these findings on cancer research. The chapters are written by internationally leading experts in the field. They provide an updated view on how the cell cycle is regulated in vivo, and about the involvement of cell cycle regulators in cancer.
Download or read book CDK Independent Initiation of the S Cerevisiae Cell Cycle Analysis of BCK2 written by Nazareth Bastajian and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Download or read book Cell Cycle Control written by Michele Pagano and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addressing the regulation of the eukaryotic cell cycle, this book brings together experts to cover all aspects of the field, clearly and unambiguously, delineating what is commonly accepted in the field from the problems that remain unsolved. It will thus appeal to a large audience: basic and clinical scientists involved in the study of cell growth, differentiation, senescence, apoptosis, and cancer, as well as graduates and postgraduates.
Download or read book Cyclins and Their Roles in Cell Cycle Progression Transcriptional Regulation and Osmostress Adaptation in Saccharomyces Cerevisiae written by Lotte Teufel and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of G1 Phase Progression written by Johannes Boonstra and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this contribution, several specialists describe the current knowledge on the molecular networks that regulate cell cycle progression, with an emphasis on the G1 phase of the cell cycle. The first part of Regulation of G1 Phase Progression is concerned with the individual molecules that form the network, including cyclins, cyclin-dependent kinases, inhibitors of these kinases and retinoblastoma and p53. The second section describes the signaling cascades by which external factors influence the cell cycle network, including mitogens, the extracellular matrix, nutrients and oxygen radicals. The last section describes the effects of specific external conditions on cell cycle progression and are presented such as serum starvation and subsequent re-addition and stress conditions (heat, osmolarity). The final two chapters describe the relation between cell cycle progression with cell differentiation and with apoptosis.
Download or read book Nutrient Regulation of Cell Growth and Cell Proliferation in Saccharomyces Cerevisiae written by Matthew Grant Slattery and published by . This book was released on 2008 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterizing Cell cycle as a Global Regulator of Stochastic Transcription and Noisy Gene Expression in S Cerevisiae written by Katie Julia Quinn and published by . This book was released on 2014 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: Even in the same environment, genetically identical cells can exhibit remarkable variability, or noise, in gene expression. This expression noise impacts the function of gene regulatory networks, depending on its origins. Hence, a prerequisite for understanding or designing gene regulatory networks is characterizing the origins and statistics of the noise. Variability has been largely attributed to the inherently stochastic nature of transcription. Expression statistics from multiple organisms are consistent with an influential model of "bursty" expression, where promoters are generally inactive but infrequently produce multiple mRNA. But fluctuations in the cell environment can also contribute, leaving the origins of noise unclear. We sought to determine the origins of noise in gene expression from the synthetic tetO promoter in S cerevisiae. We use single-molecule mRNA FISH to quantify nuclear and cytoplasmic mRNA in a population expression distribution, and models of stochastic mRNA production and degradation to infer underlying transcriptional dynamics. Rather than transcriptional bursting, we find that noise is driven by large differences in transcriptional activity between the G1 and S/G2/M stage of the cell cycle. Furthermore, we quantitatively characterize these dynamics of transcription by measuring expression in cells arrested at the G1/S and G2/M transition. Promoters activate in S/G2 with probability determined by activator level. mRNA statistics from an active promoter with a single operator are Poisson; expression with multiple operators is more variable. Promoters appear to inactivate at the M/G1 transition, with lower activator levels leading to increased probability of inactivation. Thus below a certain activator threshold, all cells are inactive in G1. mRNA processing and export introduces further variability. Similar analysis of the native, chromatin-regulated PHO5 promoter yields the same results. Hence cell-cycle driven transcription dynamics may be prevalent among regulated yeast genes. The timing of S/G2 activation suggests DNA replication and chromatin maturation may be linked to repressed transcription. Cell-cycle-linked fluctuations in expression are likely to affect gene behavior in regulatory networks. This thesis advocates the importance of cellular context in gene regulation and reveals a novel role of cell-cycle as a driver of eukaryotic transcription, advancing our understanding of stochastic transcription and noise in gene expression.
Download or read book Analysis of the SIN1 and SIN2 Gene Products and Their Role in Transcriptional Regulation in Saccharomyces Cerevisiae written by Warren David Kruger and published by . This book was released on 1991 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Transcriptional Regulation of Meiosis and Spore Morphogenesis in Saccharomyces Cerevisiae written by Shelley Chu and published by . This book was released on 1998 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt:
