Download or read book Transcriptional Regulation of the Escherichia Coli K 12 Grx Gene written by Weiyin Shen and published by . This book was released on 1997 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Gene Expression in Escherichia coli written by E. C. C. Lin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.

Download or read book Transcriptional regulation at two divergent gene promoters in Escherichia Coli K 12 written by Matthew Ian Hutchings and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Transcriptional Regulation of the Escherichia Coli K 12 Cyn Operon written by Anne-Francoise Jacqueline Lamblin and published by . This book was released on 1993 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Higher Levels of Gene Regulation in Escherichia Coli written by Karen Cooke and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Epigenetic" mechanisms result in information being carried by DNA, independent of its base sequence. These are widely studied in eukaryotic cells, but less so in bacterial contexts. DNA methylases, such as DNA adenine methyltransferase (DAM), methylate DNA at '5-GATC-3' sequences. This is known to impact upon DNA replication, DNA repair, and transcriptional regulation at some virulence promoters. Investigations in to cell methylation were achieved by digestion of plasmid pBR322 with DpnI restriction enzyme; this showed nutrient-dependent differences in methylation levels. DNA topology in wild-type and ∆dam cells was investigated by chloroquine gel electrophoresis, and this revealed a higher degree of supercoiling in ∆dam cells in the exponential phase of growth. To investigate global effects of DAM on transcription in Escherichia coli K-12, a lac28::egfp fusion was inserted at different chromosomal loci, and expression was measured using flow cytometry. Differences in gene expression between wild-type and ∆dam cells were found at these loci, with some differences depending on growth medium. At many loci, ∆dam cell populations exhibited increased fluorescence and, occasionally, less variation than wild-type cells. In further experiments, interactions between DAM and CRP (the catabolite-repression global transcription regulator protein) were explored, and found that DAM methylation could affect the action of CRP at a model promoter. My work implicates DAM methylase as a potent regulator of gene expression in Escherichia coli and an agent of cell-to-cell heterogeneity. The mechanisms by which DAM methylase affected gene regulation were probed, bioinformatically. These studies revealed that while the absolute number of GATC motifs in the locality of the lac28 promoter did not seem to correlate to overall increases in gene expression, the number of GATC sites upstream of the same promoter may correlate with changes in the variability of GFP expression. This study also highlighted that DAM regulation of some loci may be protein-dependent. Lastly, the effect of genetic position on the expression of antibiotic resistance genes was investigated in both the chromosomal and plasmid contexts. This work highlighted that chromosomal resistance cassettes may express differently, depending on their location. This in turn, yields different resistance phenotypes within the "same" bacterial strain. This concept was addditionally explored within a plasmid, whereby a transposon encoding a resistance gene was inserted randomly in to different locations in the plasmid genome. Thence, different levels of antibiotic resistance where seen that were location dependent. In sum, this work aims to further traverse the existence of so-called "higher levels" of genetic regulation within Escherichia coli.

Download or read book A Quantitative Analysis of Genetic Transcriptional and Post transcriptional Regulation in Escherichia Coli written by Thomas Edward Kuhlman and published by . This book was released on 2007 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Chapter 4, Quantitative Characteristics of Gene Regulation by Small RNA, we study quantitatively two classes of bacterial small RNAs (sRNA) in Escherichia coli. We demonstrate that sRNA provide a novel mode of gene regulation with characteristics distinct from those of protein-mediated gene regulation. These include a threshold-linear response with a tuneable threshold and a built-in capability for hierarchical cross talk.

Download or read book Decoding Transcriptional Regulatory Networks Activated During Stringent Response in Escherichia Coli written by Hemali Bharat Patel and published by . This book was released on 2009 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The stringent response is a response network in bacteria that is triggered by nutrient limitation and involves a global reprogramming of gene transcription, orchestrated largely by a signaling molecule guanosine tetraphosphate (ppGpp). Despite decades of studies on individual genes perturbed during the stringent response in Escherichia coli, the characteristic transcriptional profile and the role of ppGpp in affecting global transcriptional changes during the stringent response had remained to be determined systematically. We provoked the stringent response, in the strain E. coli K-12, by multiple induction pathways and identified the core transcriptional profile that is characteristic of the cellular response to ppGpp accumulation. By comparison of wild type and ppGpp-deficient E. coli mutant strains, we identified the gene expression changes that are specifically dependent upon ppGpp. We found six transcriptional regulators that are recruited in the core stringent response. We show the regulatory targets of one of these six regulators, RpoS. Over 80% of transcriptional regulation was directly or indirectly dependent upon the general stress response sigma factor, RpoS, even in presence of ppGpp. Our findings suggest that ppGpp alone is not capable of mounting a global stringent response, and it relies heavily on transcriptional regulators such as RpoS. Existing regulatory models for ppGpp-mediated regulation suggest a global redistribution of RNA polymerase (RNAP), upon binding with ppGpp, during stringent response. We have shown evidence for such redistribution of RNAP in presence of ppGpp by conducting ChIP-chip analysis of ppGpp-rich and ppGpp-deficient cells with an antibody specific to the alpha-subunit of RNAP. We also correlated transcriptional changes with a phenotype of increased viability in acidic medium, and demonstrated that it was dependent upon both ppGpp and RpoS. We have shown that ppGpp enhances long-term survival, up to two hours, in acid. Our results reveal the significance of stringent response in development of phenotypes that facilitate adaptation in acidic host environments for E. coli.

Download or read book Transcriptional Regulation of the Acs Gene of Escherichia Coli written by Christine M. Beatty and published by . This book was released on 2001 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Transcriptional Regulation of the IlvB Operon of Escherichia Coli K 12 written by Craig Arnold Hauser and published by . This book was released on 1984 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Regulation of PyrC Gene Expression in Escherichia Coli K 12 written by Helen Ruth Wilson and published by . This book was released on 1991 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Expression and Regulation of the PyrC Gene in Escherichia Coli K 12 written by Junke Liu and published by . This book was released on 1994 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Structure and Growth Rate Regulation of the PdxA and PdxB Pyridoxal Phosphate Biosynthetic Genes of Escherichia Coli K 12 written by Benjamin Borja Roa and published by . This book was released on 1991 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dual Two component Regulatory Systems Interact to Control Nitrate and Nitrite Regulation of Anaerobic Respiratory Gene Expression in Escherichia Coli K 12 written by Ross S. Rabin and published by . This book was released on 1994 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of the GlpFK Operon of Escherichia Coli K 12 and Characterization of it Gene Products written by Deborah L. Weissenborn and published by . This book was released on 1990 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Transcription Regulation and Genetic Structure of the IlvGEDA Operon in Escherichia Coli K12 written by Craig W. Adams and published by . This book was released on 1983 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Tricarboxylic Acid Cycle Genes in Escherichia Coli written by Soon-Jung Park and published by . This book was released on 1994 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Chemical Abstracts written by and published by . This book was released on 2002 with total page 2626 pages. Available in PDF, EPUB and Kindle. Book excerpt: