Download or read book Three Dimensional Confocal Microscopy Volume Investigation of Biological Specimens written by and published by Academic Press. This book was released on 2012-12-02 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. - Volume investigation - Three-dimensional reconstruction - Fluroescent probe design - Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology - Confocal data display and analysis - Twenty-eight pages of color

Download or read book Three Dimensional Electron Microscopy written by and published by Academic Press. This book was released on 2019-07-18 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the Three-Dimensional Electron Microscopy technique

Download or read book Cell Biological Applications of Confocal Microscopy written by and published by Elsevier. This book was released on 2003-01-04 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). - Includes detailed protocols accessible to new users - Details how to set up and run a "Confocal Microscope Core Facility" - Contains over 170 figures

Download or read book Basic Confocal Microscopy written by Robert L. Price and published by Springer Science & Business Media. This book was released on 2011-04-11 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors’ five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy.

Download or read book Handbook of Biological Confocal Microscopy written by James Pawley and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 639 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.

Download or read book Fluorescence Probes In Oncology written by Joseph G Hirschberg and published by World Scientific. This book was released on 2002-05-20 with total page 735 pages. Available in PDF, EPUB and Kindle. Book excerpt: This unique book provides a comprehensive description of fluorescence probes and the methodology for the study and diagnostics of oncology. The material is drawn directly from the work of pioneer researchers in cell biology and pathology, and offers a perspective of their most crucial investigations and lifetime experiences; it also opens new horizons on future developments in fundamental methods and diagnostics relevant to cellular physiopathology. Researchers in cell pathology have contributed a broad range of spectral and fluorescence images which most appropriately supplement the information derived from Virchow style microscope slides (these still remain valid after more than 150 years, and a considerable body of knowledge and interpretation can be built around them). The text contains about 100 colour pictures, adding great value to the book./a

Download or read book Computational Methods for Three Dimensional Microscopy Reconstruction written by Gabor T. Herman and published by Springer Science & Business Media. This book was released on 2014-01-29 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy and X-ray tomography can be captured in the same mathematical framework, leading to closely-related computational approaches, but the methodologies differ in detail and hence pose different challenges. The editors of this volume, Gabor T. Herman and Joachim Frank, are experts in the respective methodologies and present research at the forefront of biological imaging and structural biology. Computational Methods for Three-Dimensional Microscopy Reconstruction will serve as a useful resource for scholars interested in the development of computational methods for structural biology and cell biology, particularly in the area of 3D imaging and modeling.

Download or read book Fluorescence Confocal Laser Scanning Microscopy for Three dimensional Imaging of Living Biological Specimens written by David Ray Sandison and published by . This book was released on 1993 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Digital Image Analysis of Microbes written by M. H. F. Wilkinson and published by John Wiley & Sons. This book was released on 1998-06-08 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing specific knowledge in the theory of image analysis, optics, fluorescence, and imaging devices in biomedical laboratories, this timely and indispensable volume focuses on the theory and applications of detection, morphometry, and motility measurement techniques applied to bacteria, fungi, yeasts and protozoa.

Download or read book Cell Imaging Techniques written by Douglas J. Taatjes and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 505 pages. Available in PDF, EPUB and Kindle. Book excerpt: A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Download or read book Unbiased Stereology written by Vyvyan Howard and published by Garland Science. This book was released on 2004-11-15 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unbiased Stereology, Second Edition is a practical guide to making unbiased 3-D measurements via the microscope. Only those stereological techniques which have been tried and tested by real application are included. Although this technology is essentially mathematical and statistical, the authors do not immerse the reader in complex analysis, but rather provide simple heuristic explanations and references to the original proof, and illustrate the theory by analogies drawn from everyday experience. To give practical experience in application of the techniques, exercises are provided at the end of each chapter, complete with detailed worked answers.

Download or read book 3D Imaging in Medicine Second Edition written by Jayaram K. Udupa and published by CRC Press. This book was released on 2023-08-18 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a quick and systematic presentation of the principles of biomedical visualization and three-dimensional (3D) imaging. Topics discussed include basic principles and algorithms, surgical planning, neurosurgery, orthopedics, prosthesis design, brain imaging, cardio-pulmonary structure analysis and the assessment of clinical efficacy. Students, scientists, researchers, and radiologists will find 3D Imaging in Medicine a valuable source of information for a variety of actual and potential clinical applications for 3-D imaging.

Download or read book Confocal Microscopy written by and published by Elsevier. This book was released on 1999-09-07 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Download or read book Collected Works Of Shinya Inoue Microscopes Living Cells And Dynamic Molecules With Dvd rom written by Shinya Inoue and published by World Scientific. This book was released on 2008-07-18 with total page 1019 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book collects the publications of Shinya lnoué, pioneering cell biophysicist and winner of the 2003 International Prize for Biology. The articles cover the discovery, and elucidate the behavior in living cells, of the dynamic molecular filaments which organize the cell and play a central role in cell division. Other articles report on the development of microscopes, especially those using polarized light and digital image enhancement, which make possible studies of the ever-changing molecular architecture directly in living cells. This book also contains many high quality photo-micrographs as well as an appended DVD with an extensive collection of video movies of active living cells. After training in Tokyo and at Princeton University, Dr Inoué has held teaching positions at the University of Washington, Tokyo Metropolitan University, University of Rochester, Dartmouth Medical School, and University of Pennsylvania. He is a member of the U.S. National Academy of Sciences and currently holds the title of Distinguished Scientist at the Marine Biological Laboratory in Woods Hole, Massachusetts.

Download or read book Confocal and Two Photon Microscopy written by Alberto Diaspro and published by John Wiley & Sons. This book was released on 2001-11-22 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Confocal and Two-Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy. Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring: * In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications * Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules * Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references * Companion FTP site with full-color photographs * The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.

Download or read book Emerging Tools for Single Cell Analysis written by Gary Durack and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,

Download or read book Using The Biological Literature written by Diane Schmidt and published by CRC Press. This book was released on 2001-12-06 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in th