Download or read book The Regulation of Late G1 phase Specific Transcription in Saccharomyces Cerevisiae written by David Edward Lee and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of G1 Phase Progression written by Johannes Boonstra and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this contribution, several specialists describe the current knowledge on the molecular networks that regulate cell cycle progression, with an emphasis on the G1 phase of the cell cycle. The first part of Regulation of G1 Phase Progression is concerned with the individual molecules that form the network, including cyclins, cyclin-dependent kinases, inhibitors of these kinases and retinoblastoma and p53. The second section describes the signaling cascades by which external factors influence the cell cycle network, including mitogens, the extracellular matrix, nutrients and oxygen radicals. The last section describes the effects of specific external conditions on cell cycle progression and are presented such as serum starvation and subsequent re-addition and stress conditions (heat, osmolarity). The final two chapters describe the relation between cell cycle progression with cell differentiation and with apoptosis.
Download or read book Identification and Characterizaton of Genes Required for Cell Viability in the Absence of the Swi6 Protein in Saccharomyces Cerevisiae written by Neil N. Macpherson and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 'Saccharomyces cerevisiae', the Swi6 protein is a component of two transcription factors, SBF and MBF, that promote gene expression in late G1 phase of the cell cycle. In SBF, Swi6 interacts with the DNA-binding protein Swi4 to promote activation of the G1 cyclin-encoding genes ' CLN1, CLN2, PCL1' and 'PCL2', the 'HO' endonuclease-coding gene and cell wall biosynthesis genes. In MIBF, Swi6 is associated with a related DNA-binding protein, Mbp1, and activates transcription of DNA synthesis genes. Although SBF and MBF are required for cell viability, ' SWI6' is not an essential gene. I performed a synthetic lethal screen to identify genes that are required for viability in the absence of 'SWI6'. I identified ten complementation groups of 's_wi6'-dependent l_ethal m_utants, designated 'SLM1' through 'SLM10'. Members of the 'SLM2' complementation group may contain mutations in ' SWI2', since a 'slm2 swi6' mutant is rescued by overexpression of 'SWI2'. SWI2 encodes a component of the Swi/Snf chromatin-remodefing complex. The 'slm10' mutants contain mutations that are allelic to the 'PHO85' gene. I was most interested in mutants showing a cell cycle arrest phenotype; ' swi6' strains carrying mutations in 'slm7' and ' slm8' arrested in G1 phase under restrictive conditions. Analysis of the transcript levels of cell cycle-regulated genes in 'slm7' mutant strains revealed defects in regulation of a subset of cyclin genes. Complementation and allelism tests showed that 'SLM7' is allelic to the 'TAF17' gene, which encodes a protein that is a component of the basal transcription factor TFIID and a histone acetyl transferase complex. Sequencing showed that the 'slm7-1' allele of 'TAF17 ' is predicted to encode a version of Taf17 that is truncated within a highly conserved region. The cell cycle and transcriptional defects caused by the 'slm7-1' allele of 'TAF17' are consistent with the role of TAFs as modulators of transcriptional activation, and may reflect a role for 'TAF17' in modulating transcriptional activation by SBF and MBF. My discovery of a genetic interaction between 'TAF17 ' and 'SWI6' may reflect a novel role for basal transcription factors in G1-specific transcription, and may implicate chromatin remodeling in cell cycle-dependent transcription.
Download or read book Connections Between Growth and Division written by Laura Lynn Newcomb and published by . This book was released on 2001 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Cycle Regulation written by Philipp Kaldis and published by Results and Problems in Cell Differentiation. This book was released on 2006-06-26 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a state-of-the-art summary of the latest achievements in cell cycle control research with an outlook on the effect of these findings on cancer research. The chapters are written by internationally leading experts in the field. They provide an updated view on how the cell cycle is regulated in vivo, and about the involvement of cell cycle regulators in cancer.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book DNA Replication written by Hisao Masai and published by Springer. This book was released on 2018-01-22 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.
Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.
Download or read book Nutrient Regulation of Cell Growth and Cell Proliferation in Saccharomyces Cerevisiae written by Matthew Grant Slattery and published by . This book was released on 2008 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of the SIN1 and SIN2 Gene Products and Their Role in Transcriptional Regulation in Saccharomyces Cerevisiae written by Warren David Kruger and published by . This book was released on 1991 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Spatio Temporal Regulation of Cell Cycle Transcription written by Umut Eser and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Start checkpoint regulates cell cycle commitment and associated transcription in the budding yeast, Saccharomyces cerevisiae. It was previously shown that commitment to cell division corresponds to activating the positive feedback loop of G1 cyclins controlled by the transcription factors SBF and MBF. Around this pivotal cell cycle event, over 300 genes (G1/S regulon) are expressed to facilitate the G1/S transition. Despite its importance, little was known about distinct temporal regulation within the G1/S regulon. We found that SBF and MBF target genes have a well-defined distribution of transcriptional activation times. We also showed that activation of G1 cyclins precedes the activation of the bulk of the G1/S regulon, which we named 'feedback-first' regulation. In budding yeast, feedback-first regulation ensures that commitment to cell division occurs before large-scale changes in transcription. Thus, the transition can be viewed as a two-step process whereby the decision to divide precedes synthesis of the cellular machinery required for division. Furthermore, we found that feedback-first regulation is conserved in the related yeast S. bayanus as well as human cells. This finding highlighted the importance of understanding the molecular mechanisms through which co-regulated genes can have distinct activation dynamics. We showed that timing is partially explained by the combinatorial use of SBF and MBF transcription factors, which implement a logical OR function for gene activation. In addition to combinatorial use of transcription factors, we analyzed genome-wide chromosome conformation capture data to examine the potential link between the timing of gene expression and 3-D genome architecture. The early-activated genes of the G1/S regulon are significantly enriched for the number of physical contacts to the rest of the genome. Further analysis revealed two main clusters, whose interactions co-vary and whose activation time distributions are distinct. Taken together, these our work explains a significant amount of timing variation within cell cycle-dependent gene expression. Thus, we concluded that the cell utilizes both genome architecture and the combinatorial use of transcription factors to implement feedback-first regulation ensuring that commitment to cell division precedes genome-wide cell cycle-dependent transcription.
Download or read book Identification and Analysis of Regulators of GI Progression in Saccharomyces Cerevisiae written by Joseph Paul Ogas and published by . This book was released on 1992 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Systematic Identification of Regulators of Cell Cycle Commitment and a Dynamic Transcriptional Network that Communicates Growth Potential to Ribosome Synthesis and Critical Cell Size in Budding Yeast microform written by Paul Conrad Jorgensen and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2004 with total page 778 pages. Available in PDF, EPUB and Kindle. Book excerpt: Size homeostasis requires that proliferating cells co-ordinate their growth and division. In Saccharomyces cerevisiae, this co-ordination occurs in late G1 phase at the point of commitment to cell division termed Start; traversal through Start requires that cells have grown to a critical cell size. The cell size threshold is flexible and is set higher in rich nutrient conditions and in proportion to ploidy. Despite its importance, Start remains poorly understood, in large part because the cell size phenotype has been refractory to conventional genetics. The recent construction of & sim;6000 yeast strains deleted for every predicted open reading frame allowed me to systematically identify genes whose deletion confers abnormally small or large cell size. These screens identified 40 potential Start regulators, including Whi5, the long postulated but previously unidentified target of Cln3-Cdc28 kinase at Start. The smallest deletion strains carried disruptions in SFP1 or SCH9. SFP1 encodes a zinc-finger transcription factor while SCH9 encodes a protein kinase, but neither gene product had been well characterised. Numerous experiments demonstrated that although Sfp1 and Sch9 are important for cell growth, they are also bona fide Start repressors. Remarkably, both Sfp1 and Sch9 activated the RP and Ribi regulons, two expansive transcriptional programmes whose expression is rate-limiting for ribosome production. This finding meshed nicely with the cell size screens, as my shortlist of potential Start repressors included 15 ribosome biogenesis factors. I elaborated a control network including Sfp1, Sch9, and the transcription factors Rgm1, Fhl1, and Ifh1 at RP promoters. Sfp1 and Sch9 are controlled by nutrient status at the level of nuclear localisation and abundance, respectively, and appear to tailor a cell's ribosome production to its growth potential. Overall, my data argues that the rate of ribosome biogenesis, dictated by nutrients via Sfp1 and Sch9, modulates the critical cell size threshold at Start by a mechanism that is independent of the known upstream regulators Cln3, Bck2, and Whi5. My work has allowed for a more complete molecular characterisation of Start, elucidated a dynamic transcriptional control network for the RP and Ribi regulons, and illuminated connections between ribosome biogenesis and Start.
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by and published by . This book was released on 2014 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book G1 S Transcriptional Regulation in Saccharomyces Cerevisiae Integrates Cell Cycle Progression and Genome Stability written by Michael Ross Harris and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Systems Biology of Cancer written by Sam Thiagalingam and published by Cambridge University Press. This book was released on 2015-04-09 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of the current systems biology-based knowledge and the experimental approaches for deciphering the biological basis of cancer.
Download or read book Transcriptional Regulation of the G1 S Cell Cycle Transition in Saccharomyces Cerevisiae written by Herman Wijnen and published by . This book was released on 2000 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
