Download or read book Isolation and Characterization of a Temperature sensitive Mutation in the 88 89 Minutes of the Escherichia Coli Chromosome written by Issmat Ibrahim Kassem and published by . This book was released on 2003 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: In an attempt to isolate a temperature sensitive allele of ftsN, chemical random mutagenesis was performed using hydroxylamine. SMM 12 a temperature sensitive filamentous strain was successfully isolated. However, it did not carry an ftsN(Ts) allele. Transforming with different pasmids carrying cloned genes and random genomic plasmid libraries have yielded no complementation. Using P I -mediated transduction, the location of the mutation was found to be at the 88-89 minute region on the E. coli chromosome. This finding represents the discovery of an unprecedented gene at the 88- 89 minute region that blocks cell division. in addition, we report in this work the isolation of the first temperature-sensitive mutant that is suppressed by L-glutamate. Although the precise identity of the mutated gene remains illusive, all data produced by this work point out that we may have isolated a previously unknown player in the cell- division protein machinery in E. coli.