Download or read book The Fluctuating Enzyme written by G. Rickey Welch and published by Wiley-Interscience. This book was released on 1986 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have emerged a number of theoretical models of enzyme action which maintain that classes of fluctuations in the protein structure provide means for generating high energy-free events at the active center. The purpose of the present volume is to review these theoretical pursuits.

Download or read book A Study of Enzymes written by Stephen A. Kuby and published by CRC Press. This book was released on 1990-11-16 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive monograph consists of two parts: Volume I, entitled Enzyme Catalysis, Kinetics, and Substrate Binding; and Volume II, entitled Mechanism of Enzyme Action. Volume I focuses on several aspects of enzyme catalytic behavior, their steady-state and transient-state kinetics, and the thermodynamic properties of substrate binding. Packed with figures, tables, schemes, and photographs, this volume contains over 1,000 references, including references regarding enzymology's fascinating history. This comprehensive book is of particular interest to enzymology students, teachers, and researchers. Volume II presents selected "cutting edge" examples of techniques and approaches being pursued in biochemistry. This up-to-date resource includes 11 chapters, which illustrate important theoretical and practical aspects of enzyme mechanisms. It also features selected examples in which today's most important techniques, ideas, and theories are used to elaborate on the intricate nature of enzyme action mechanisms. This particular volume provides important information for both the novice and the seasoned investigator.

Download or read book Understanding Enzymes written by Allan Svendsen and published by CRC Press. This book was released on 2016-04-27 with total page 884 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also prese

Download or read book The Enzyme Catalysis Process written by A. Cooper and published by Springer Science & Business Media. This book was released on 2013-12-11 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume represents the proceedings of a NATO Advanced Studies Instituteheld near Barga (Italy), July 11-23, 1988, involving over 90 participants from more than twelve countries of Europe, North America and elsewhere. It was not our intention at this meeting to present a complete up-to-the-minute review of current research in enzyme catalysis but t·ather, in accord wi th the intended spiri t of NATO ASis, to gi ve an opportunity for advanced students and researchers in a wide variety of disciplines to meet tagether and study the problern from different points of view. Hence the lectures cover topics rauging from the purely theoretical aspects of chemical reaction kinetics in condensed matter through practical experimental approaches to enzyme structure, dynamics and mechanism, including the new experimental opportunities arising from genetic engineering techniques. Our approachwas unashamedly physical, both because the more biochemical aspects of enzymology are amply covered elsewhere and because progress in our understanding and application of the molecular basis of enzymic processes must ultimately come from advances in physical knowledge. We tried to cover as wide a spectrum as possible, and succeeded in gathering an expert and enthusiastic team of speakers, but there . are some inevitable omissions. In particular, and with hindsight, our discussions might have been enriched by more detailed coverage of general aspects of chemical catalysis - but readers requiring this background should find adequate references herein.

Download or read book Kinetics of Enzyme Action written by Ross L. Stein and published by John Wiley & Sons. This book was released on 2011-08-08 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Download or read book Enzymes and Coenzymes Advances in Research and Application 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 1417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes and Coenzymes: Advances in Research and Application: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Enzymes and Coenzymes. The editors have built Enzymes and Coenzymes: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Enzymes and Coenzymes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Enzymes and Coenzymes: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Download or read book Mobility and Proximity in Biological Membranes written by S. Damjanovich and published by CRC Press. This book was released on 2018-01-18 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell surface membranes have long been characterized as two-dimensional fluids whose mobile components are randomized by diffusion in the plane of the membrane bilayer. Recent research has indicated that cell surface membranes are highly organized and ordered and that important functional units of membranes appear as arrays of interacting molecules rather than as single, freely diffusing molecules. Mobility and Proximity in Biological Membranes provides an overview of the results obtained from biophysical methods for probing the organization of cell surface membranes. These results are presented in the context of detailed treatments of the theory and the technical demands of each of the methods. The book describes a versatile and easily applied mode for investigating molecular proximities in plasma membranes in a flow cytometer. Its analysis of lipid fluidity and viscosity of membranes and the rotational mobility of proteins offers intimate insight into the physical chemistry of biological membranes. The electrophysiology of lymphocytes is presented with focus on its importance in different diseases. New techniques are described, and new data, new possibilities, and future trends are presented by world experts. This book's chapters can serve both as guides to the existing literature and as starting points for new experiments and approaches associated with problems in membrane function.

Download or read book Dynamics in Enzyme Catalysis written by Judith Klinman and published by Springer. This book was released on 2013-09-14 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: Christopher M. Cheatum and Amnon Kohen, Relationship of Femtosecond–Picosecond Dynamics to Enzyme-Catalyzed H-Transfer. Cindy Schulenburg and Donald Hilvert, Protein Conformational Disorder and Enzyme Catalysis. A. Joshua Wand, Veronica R. Moorman and Kyle W. Harpole, A Surprising Role for Conformational Entropy in Protein Function. Travis P. Schrank, James O. Wrabl and Vincent J. Hilser, Conformational Heterogeneity Within the LID Domain Mediates Substrate Binding to Escherichia coli Adenylate Kinase: Function Follows Fluctuations. Buyong Ma and Ruth Nussinov, Structured Crowding and Its Effects on Enzyme Catalysis. Michael D. Daily, Haibo Yu, George N. Phillips Jr and Qiang Cui, Allosteric Activation Transitions in Enzymes and Biomolecular Motors: Insights from Atomistic and Coarse-Grained Simulations. Karunesh Arora and Charles L. Brooks III, Multiple Intermediates, Diverse Conformations, and Cooperative Conformational Changes Underlie the Catalytic Hydride Transfer Reaction of Dihydrofolate Reductase. Steven D. Schwartz, Protein Dynamics and the Enzymatic Reaction Coordinate.

Download or read book Molecular Science of Fluctuations Toward Biological Functions written by Mazahide Terazima and published by Springer. This book was released on 2016-02-09 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this monograph, the importance of fluctuations for biological reactions is discussed from various points of view. Understanding the biological reactions at the molecular level is one of the major targets in many scientific fields, including not only basic biology but also physics, physical chemistry, and medical science. One of the key factors in the process is “fluctuation”. Thermal energy causes biological molecules to be in constant fluctuation even while they are carrying out their biological functions. How do biological systems overcome the thermal fluctuations to realize the physiologically relevant reactions? Scientists in a number of fields—physics, chemistry, pharmacology, medicine, and others—have contributed chapters that elucidate the nature of the fluctuations and the relationship between fluctuations and biological functions. The fluctuations discussed in this volume are detected by the transient grating method, nuclear magnetic resonance, X-ray diffraction and scattering, and computer simulation, among other methods. The book presents various results of the studies of fluctuations in biological processes that were obtained with these methods by the leading scientists in their fields.

Download or read book Advances in Protein Chemistry written by and published by Academic Press. This book was released on 1991-06-13 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 41 in a series intended for biochemists, molecular biologists, cell biologists, and biophysicists.

Download or read book Comprehensive Natural Products III written by and published by Elsevier. This book was released on 2020-07-22 with total page 4266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Natural Products III, Third Edition, Seven Volume Set updates and complements the previous two editions, including recent advances in cofactor chemistry, structural diversity of natural products and secondary metabolites, enzymes and enzyme mechanisms and new bioinformatics tools. Natural products research is a dynamic discipline at the intersection of chemistry and biology concerned with isolation, identification, structure elucidation, and chemical characteristics of naturally occurring compounds such as pheromones, carbohydrates, nucleic acids and enzymes. This book reviews the accumulated efforts of chemical and biological research to understand living organisms and their distinctive effects on health and medicine and to stimulate new ideas among the established natural products community. Provides readers with an in-depth review of current natural products research and a critical insight into the future direction of the field Bridges the gap in knowledge by covering developments in the field since the second edition published in 2010 Split into 7 sections on key topics to allow students, researchers and professionals to find relevant information quickly and easily Ensures that the knowledge within is easily understood by and applicable to a large audience

Download or read book Protein Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.

Download or read book Enzyme Physics written by Mikhail V. Vol kenshtein and published by Springer. This book was released on 2013-12-11 with total page 205 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book treats a new, far-from-fully-developed area of molecular biophysics-enzyme physics. An attempt is made to survey this field, but primary consideration is given tothreeprob lems under investigation in the Polymer Structure Labaratory of the Institue of High-Molecular Compounds, Academy of Seiences ofthe USSR. The first problern is the genetic coding of the biologically fun.ctional structure of proteins. Its solution is based on physical theories of hydrophobic interactions. The second problern is the conformational properties of pro teins as the factor governing enzyme activity. The most direct methods for experimental investigation of questions in this area are optical, principally those involving natural and magnetic rota tion of the plane of polarization. A substantial portion of the book concerns optical activity; the Faraday effect is discussed in an appendix. The third problern is the manifestation of the cooperative properties of enzymes in the kinetics of enzymatic reactions and the solution of complex kinetic problems. This problern is espe cially pressing in connection with research on allosteric enzymes, which are responsible for feedback in metabolic processes. An appendix describes a new method for solving kinetic problems, based on the theory of graphs. This volume extends and details certain of the ideas ex pressed in my previous book, Molecules and Life: An Introduction to Molecular Biophysics, which was published in this series in 1965.

Download or read book Quantitative Understanding of Biosystems written by Thomas M. Nordlund and published by CRC Press. This book was released on 2019-04-16 with total page 870 pages. Available in PDF, EPUB and Kindle. Book excerpt: Praise for the prior edition "The author has done a magnificent job... this book is highly recommended for introducing biophysics to the motivated and curious undergraduate student." ―Contemporary Physics "a terrific text ... will enable students to understand the significance of biological parameters through quantitative examples―a modern way of learning biophysics." ―American Journal of Physics "A superb pedagogical textbook... Full-color illustrations aid students in their understanding" ―Midwest Book Review This new edition provides a complete update to the most accessible yet thorough introduction to the physical and quantitative aspects of biological systems and processes involving macromolecules, subcellular structures, and whole cells. It includes two brand new chapters covering experimental techniques, especially atomic force microscopy, complementing the updated coverage of mathematical and computational tools. The authors have also incorporated additions to the multimedia component of video clips and animations, as well as interactive diagrams and graphs. Thomas Nordlund is professor emeritus in the Department of Physics at The University of Alabama at Birmingham. He is an elected fellow of the American Physical Society and has been studying biomolecular dynamics for over thirty years. Peter M. Hoffmann is a professor in the Department of Physics and Astronomy at Wayne State University in Detroit, Michigan, where he founded the biomedical physics program. He has been involved in soft matter and biophysics research for twenty-five years, and earned his PhD in materials science and engineering from Johns Hopkins University.

Download or read book Handbook of Single Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Download or read book Frontiers in Computational and Systems Biology written by Jianfeng Feng and published by Springer Science & Business Media. This book was released on 2010-06-14 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological and biomedical studies have entered a new era over the past two decades thanks to the wide use of mathematical models and computational approaches. A booming of computational biology, which sheerly was a theoretician’s fantasy twenty years ago, has become a reality. Obsession with computational biology and theoretical approaches is evidenced in articles hailing the arrival of what are va- ously called quantitative biology, bioinformatics, theoretical biology, and systems biology. New technologies and data resources in genetics, such as the International HapMap project, enable large-scale studies, such as genome-wide association st- ies, which could potentially identify most common genetic variants as well as rare variants of the human DNA that may alter individual’s susceptibility to disease and the response to medical treatment. Meanwhile the multi-electrode recording from behaving animals makes it feasible to control the animal mental activity, which could potentially lead to the development of useful brain–machine interfaces. - bracing the sheer volume of genetic, genomic, and other type of data, an essential approach is, ?rst of all, to avoid drowning the true signal in the data. It has been witnessed that theoretical approach to biology has emerged as a powerful and st- ulating research paradigm in biological studies, which in turn leads to a new - search paradigm in mathematics, physics, and computer science and moves forward with the interplays among experimental studies and outcomes, simulation studies, and theoretical investigations.

Download or read book Measurement and Analysis of Kinetic Isotope Effects written by and published by Academic Press. This book was released on 2017-09-12 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD. Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions. Written by pioneers of modern isotope effect research Is the only collection of modern kinetic isotope effect methods currently available