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Book New Viral Vectors for the Expression of Antigens and Antibodies in Plants

Download or read book New Viral Vectors for the Expression of Antigens and Antibodies in Plants written by Zun Liu and published by . This book was released on 2009 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants viruses are increasingly being examined as alternative recombinant protein expression systems. Future development of plant virus expression vectors needs to focus on the most important economic hosts, namely cereals and legumes, to develop tools to aid breeding of such hosts and systems for edible vaccine production. Sunn hemp mosaic virus (SHMV) is a tobamovirus, which infects leguminous plants. This work reports on new SHMV-based viral vectors for high yield of target proteins in legumes. In the SHEC vector series, the coat protein gene of SHMV was substituted by a reporter gene. In the SHAC vector series, the coat protein was substituted by a reporter gene and the coat protein gene from another tobamovirus, tobacco mild green mosaic virus (TMGMV). Co-agroinoculation of SHEC/GFP with an RNA silencing suppressor resulted in high levels of local GFP expression by 3 days post inoculation. Co-agroinoculation with SHAC/GFP led to systemic fluorescence in 12-19 dpi. Foxtail mosaic virus (FoMV) is a species of the group Potexvirus, which infects cereal plants. A new viral vector series named FECT was constructed by eliminating the triple gene block and coat protein genes, reducing the viral genome by 29%. Interestingly, agroinoculation of the vector alone results in only slight transient expression, whereas co-inoculation with silencing suppressor genes allows for GFP expression of 40% total soluble protein. Full-sized HC and LC components of an anti-langerin IgG, each carried by a separate FECT vector, expressed and folded into immunologically functional antibody upon co-inoculation. This may prove a useful and environmentally safe vector for both transient expression and perhaps transgenic plants. Mountain cedar (Juniperus ashei) pollen causes severe allergies in Texas and the central USA. Jun a 1 is the dominant allergen protein of mountain cedar pollen and would be a good allergen vaccine candidate. Recombinant Jun a 1 was expressed in Nicotiana benthamiana using an agroinoculation-compatible tobacco mosaic virus vector and isolated in good quantity from the apoplast by vacuum infiltration (100 [mu]g/g leaf material). The recombinant protein samples were characterized. Pectate lyase activity was detected from plant extracts, suggesting the cause of severe necrotic reaction in plants.

Book Genetic Engineering of Potyvirus Resistance Using Constructs Derived from the Zucchini Yellow Mosaic Virus Coat Protein Gene

Download or read book Genetic Engineering of Potyvirus Resistance Using Constructs Derived from the Zucchini Yellow Mosaic Virus Coat Protein Gene written by Guowei Fang and published by . This book was released on 1992 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Viral Resistance in Plants  Viral Coat Proteins

Download or read book Viral Resistance in Plants Viral Coat Proteins written by and published by . This book was released on 1996 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Viral Resistance in Plants Viral Coat Proteins

Download or read book Viral Resistance in Plants Viral Coat Proteins written by Raymond Dobert and published by DIANE Publishing. This book was released on 1997-02 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt: A bibliography containing 181 citations in English that have been derived from the National Agricultural Library's AGRICOLA database. Entries include books, journal and audiovisuals. Citations include, where applicable: title, author, publisher, NAL call number, place and date of publication, volume and issue number, no. of pages, media format length, and description. Many entries include abstracts. Indexed by author and subject.

Book Plant Resistance to Viruses

Download or read book Plant Resistance to Viruses written by David Evered and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concern about the environmental consequences of the widespread use of pesticides has increased, and evidence of pesticide-resistant virus vectors have continued to emerge. This volume presents a timely survey of the mechanisms of plant resistance and examines current developments in breeding for resistance, with particular emphasis on advances in genetic engineering which allow for the incorporation of viral genetic material into plants. Discusses the mechanisms of innate resistance in strains of tobacco, tomato, and cowpea; various aspects of induced resistance, including the characterization and roles of the pathogenesis-related proteins; antiviral substances and their comparison with interferon; and cross-protection between plant virus strains. Also presents several papers which evaluate the status of genetic engineering as it relates to breeding resistant plants. Among these are discussions of the potential use of plant viruses as gene vectors, gene coding for viral coat protein, satellite RNA, and antisense RNA, and practical issues such as the durability of resistant crop plants in the field.

Book Molecular Biology of the Biological Control of Pests and Diseases of Plants

Download or read book Molecular Biology of the Biological Control of Pests and Diseases of Plants written by Muthukumaran Gunasekaran and published by CRC Press. This book was released on 2020-01-29 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: While many books are available on biological control, this is the only book to detail the application of molecular biology to control of pests and diseases. Each chapter deals with a different pathogen and the application of new molecular biological techniques to the biocontrol of the pathogen. This new reference presents the most comprehensive list of organisms available. Internationally respected experts discuss viruses, bacteria, fungi, nematodes, protozoa, weeds, and insects. Types of control methods are described, and techniques commonly used in molecular biology to identify the etiological agents, diagnose diseases, and develop control methods are reviewed.

Book Recognition and Response in Plant Virus Interactions

Download or read book Recognition and Response in Plant Virus Interactions written by Ronald S.S. Fraser and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mechanisms of resistance to plant viruses are diverse, and probably involve different types of recognition events. Often, a cascade of changes affecting broader aspects of defence and metabolism is switched on progressively after the initial recognition event. Virulence, i.e. resistence-breaking behaviour of the virus, involves a failure or alteration of recognition or subsequent signalling. Consequences of these recognition events are the ways in which the pathogenic effects on the host are exerted: formation of visible symptoms and control of plant growth. This volume offers a comprehensive coverage of the recognition and signalling events between plants and viruses whereby the particular attraction of viruses (and viroids) is that they can now be completely defined in molecular terms: they offer excellent opportunities for studying the molecular biology of signalling, and may even provide useful guidelines on how plants and cellular pathogens interact.

Book Dissertation Abstracts International

Download or read book Dissertation Abstracts International written by and published by . This book was released on 1990 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Temporal and Spatial Regulation of Plant Genes

Download or read book Temporal and Spatial Regulation of Plant Genes written by Desh Pal S Verma and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: First attempts to isolate plant genes were for those genes that are abun dantly expressed in a particular plant organ at a specific stage of devel opment. However, many important gene products are produced in a very minute quantity and in specialized cell types. Such genes can now be isolated using a variety of approaches, some of which are described in this volume. The rapid progress during the last decade in regeneration of a number of crop plants and the availability of molecular tools to introduce foreign genes in plants is allowing the engineering of specific traits of agri cultural importance. These genes must, however, be regulated in a spatial and temporal manner in order to have desired effects on plant devel opment and productivity. The habitat of plants necessitate adaptive responses with respect to the environmental changes. Starting from germination of the seed, the plant begins to sense environmental cues such as moisture, light, temperature and the presence of pathogens, and begins to respond to them. Little is known about various signal transduction pathways that lead to biochemical and morphogenetic responses, in particular, transition from vegetative to reproductive phase. With the availability of tools to generate specific mutations via transposon tagging, identification and isolation of genes affecting these processes may be facilitated. Transfer of these genes into heterologous environments will allow understanding of the complex processes that control plant development.

Book Quick Bibliography Series

Download or read book Quick Bibliography Series written by and published by . This book was released on 1976 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Genetic Engineering of Crop Plants

Download or read book Genetic Engineering of Crop Plants written by G. W. Lycett and published by Elsevier. This book was released on 2013-10-22 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic Engineering of Crop Plants is a proceeding of The 49th Nottingham Easter School in Agricultural Science, which was held at Sutton Bonington on April 17-21, 1989. This symposium discussed progress in the generation of crop species resistant to herbicides, viruses, and insects. The book discusses topics such as the genetic manipulation in plants; genetic engineering of crops for insect and herbicide resistance; the expression of heat shock gene in transgenic plants; and tuber-specific gene expression. The book also covers topics such as regulation of gene expression in transgenic tomato plants; the molecular biology of pea seed development; and the regulatory elements of maize storage protein genes. The text is recommended for experts in the field of botany, agriculture, and genetics who would like to know more about the improvement of crop plants through genetics.

Book Biotechnology Applications for Banana and Plantain Improvement

Download or read book Biotechnology Applications for Banana and Plantain Improvement written by and published by Bioversity International. This book was released on with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Plant Virology in Sub Saharan Africa

Download or read book Plant Virology in Sub Saharan Africa written by Jacqueline d'A. Hughes and published by IITA. This book was released on 2003 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Plant Viruses As Molecular Pathogens

Download or read book Plant Viruses As Molecular Pathogens written by Jawaid A. Khan and published by CRC Press. This book was released on 2001-11-08 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn to produce healthier crops and better harvests! This uniquely valuable book highlights the tremendous progress of knowledge in different areas of the field over the last decade. Here you'll find new and useful information about plant molecular virology and how the field can improve the world food situation in the coming years. The last decade has seen remarkable advances in plant virological research, owing mainly to the rapid progress made in molecular biology and genetic engineering in recent years. While recombinant DNA technology has significantly contributed to our understanding of plant viruses, new findings are being accumulated every day as reported in various publications. Plant Viruses As Molecular Pathogens is the only book to bring you all of this information--22 chapters--in a single volume, compiled by specialists around the globe! Use Plant Viruses As Molecular Pathogens to enhance your knowledge of: current virus taxonomy the molecular basis of virus transmission movement of plant viruses replication and gene expression of RNA/DNA viruses resistance to viruses molecular epidemiology recombination events and possible mechanisms molecular diversity novel aspects of plant virus detection technologies With helpful illustrations, photos, figures, models that explain viral mechanisms, and easy-to-understand reference tables, Plant Viruses As Molecular Pathogens will stimulate your thinking on this fascinating area of plant science!

Book Molecular Biology of Plant Nuclear Genes

Download or read book Molecular Biology of Plant Nuclear Genes written by Indra Vasil and published by Elsevier. This book was released on 2012-12-02 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Culture and Somatic Cell Genetics of Plants, Volume 6: Molecular Biology of Plant Nuclear Genes focuses on the spectacular and rapid advances in the molecular biology and genetics of plants. This book consists of 19 chapters. Chapters 1 to 5 describe the most commonly used approaches for the genetic transformation of plants. The light-inducible and tissue-organ-specific genes are discussed in Chapters 6 to 11. In Chapters 12 to 14, the genes regulating phytohormone synthesis, heat shock proteins, and nodulation in legume roots are treated, while in Chapters 15 to 16, the relationship between chromatin structure and gene expression and molecular biology of plant RNA viruses are analyzed. The development of transgenic plants resistant to viruses, insects, and herbicides is dealt with in the last three chapters. This volume is suitable for plant molecular biologist, genetic engineers, and researchers concerned with plant cell and tissue culture.

Book Geminivirus  Detection  Diagnosis and Management

Download or read book Geminivirus Detection Diagnosis and Management written by R.K. Gaur and published by Academic Press. This book was released on 2022-05-28 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geminivirus: Detection, Diagnosis and Management focuses on the latest techniques for managing diseases caused by these circular, single-stranded (ss) DNA genomes. The most significant impact of plant diseases in host populations is often caused by emerging diseases, whose incidence in a plant host is increasing as a result of long-term changes in their underlying epidemiology. Genetic changes in pathogen and host populations, as well as changes in host ecology and environment, are major factors contributing to disease emergence. Understanding plant virus evolution is crucial for modeling the within-host and between-host dynamics and genetics of virus populations. The book presents a comprehensive review of how these viruses develop, including contributing factors such as population bottlenecks during cell-to-cell movement, systemic colonization, or between-host transmission by different procedures. Presented in five sections—Detection and Diagnosis, Emergence and Diversity, Vector and Transmission, Virus–Host Interaction, and Disease Management, the book includes host range determinant and virulence factors involved in pathogenesis, virus–vector interactions during acquisition, retention, and transmission and evaluating management strategies to control Geminivirus. The book is an essential reference for students and researchers interested in plant virology, particularly begomoviruses, geminiviruses, and vector transmission biology. - Introduces identification and characterization of geminiviruses that infect agricultural crops, their wild relatives, and weed hosts - Discusses recombination and reassortment mechanisms influencing viral genetic diversity, virulence, and vector transmission - Explores the origin, evolution, and bottlenecks of Geminiviruses - Introduces identification and characterization of geminiviruses that infect agricultural crops, their wild relatives, and weed hosts - Discusses recombination and reassortment mechanisms influencing viral genetic diversity, virulence, and vector transmission - Explores the origin, evolution, and bottlenecks of Geminiviruses