Download or read book Molecular Methods in Plant Disease Diagnostics written by Neil Boonham and published by CABI. This book was released on 2016-04-19 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.
Download or read book Characterization of Plant Viruses written by Alangar Ishwara Bhat and published by Humana. This book was released on 2021-03-20 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses on in vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.
Download or read book Biotechnology and Plant Disease Management written by Zamir K. Punja and published by CABI. This book was released on 2007-01-01 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: As agricultural production increases to meet the demands of a growing world population, so has the pace of biotechnology research to combat plant disease. Diseases can be caused by a variety of complex plant pathogens including fungi, bacteria, viruses and nematodes, and their management requires the use of techniques in transgenic technology, biochemistry and genetics. While texts exist on specific pathogens or management practices, a comprehensive review is needed of recent developments in modern techniques and the understanding of how pathogens cause disease. This collection of studies discusses the key approaches to managing each group of pathogens within the context of recent developments in biotechnology. Broad themes include microbe-plant interactions, molecular diagnostics of plant pathogens and enhancing the resistance of plants.
Download or read book Applied Plant Virology written by L. P. Awasthi and published by Academic Press. This book was released on 2020-05-14 with total page 851 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Plant Virology: Advances, Detection, and Antiviral Strategies provides an overview on recent developments and applications in the field of plant virology. The book begins with an introduction to important advances in plant virology, but then covers topics including techniques for assay detection and the diagnosis of plant viruses, the purification, isolation and characterization of plant viruses, the architecture of plant viruses, the replication of plant viruses, the physiology of virus-infected hosts, vectors of plant viruses, and the nomenclature and classification of plants. The book also discusses defense strategies by utilizing antiviral agents and management strategies of virus and viroid diseases. With contributions from an international collection of experts, this book presents a practical resource for plant virologists, plant pathologists, horticulturalists, agronomists, biotechnologists, academics and researchers interested in up-to-date technologies and information that advance the field of plant virology. - Covers the detection, control and management of plant viruses - Discusses antiviral strategies, along with mechanisms of systemic induced resistance to enhance the defense of plants against viruses - Provides contributory chapters from expert plant virologists from different parts of the world
Download or read book A Century of Plant Virology in India written by Bikash Mandal and published by Springer. This book was released on 2017-11-28 with total page 800 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book is a compilation of research work carried out on plant viruses during past 100 years in India. Plant viruses are important constraints in Indian agriculture. Tropical and sub-tropical environments and intensive crop cultivation practices ideally favours perpetuation of numerous plant viruses and their vectors in India, which often cause wide spread crop losses. Of all the plant pathogens, studies of plant viruses have received a special attention as they are difficult to manage. A large body of literature has been published on the plant virus research from India during past 100 years; however the information is so far not available in one place. This book provides comprehensive information on the biology, molecular biology, epidemics, crop losses, diagnosis and management of viruses and viroids occurring in India. Description of properties of the viruses are provided in the chapters comprising of different genera such as Allexivirus, Begomovirus, Babuvirus, Badnavirus, Carlavirus, Carmovirus, Cucumovirus, Closterovirus, Ilavirus, Mandrivirus, Potyvirus, Tospovirus, Tungrovirus and Sobemovirus. Virus-vector research related to aphid, thrips and whitefly is discussed. The work on the management aspects of plant viral diseases has been described with reference to the conventional, antiviral and transgenic approaches. Further, the quarantine mechanism developed in India for the exclusion of viruses and vectors has also been included. The book also provides useful information about the capacity building on the research and education on Plant Virology in India. Overall, the book covers a wide range of accounts of research findings and innovations in Plant Virology in India during past 100 years. The book will be a resourceful reference to the students, scientists, agricultural professionals and policy makers.
Download or read book Plant Virus Evolution written by Marilyn J. Roossinck and published by Springer Science & Business Media. This book was released on 2008-02-23 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive look at the field of plant virus evolution. It is the first book ever published on the topic. Individual chapters, written by experts in the field, cover plant virus ecology, emerging viruses, plant viruses that integrate into the host genome, population biology, evolutionary mechanisms and appropriate methods for analysis. It covers RNA viruses, DNA viruses, pararetroviruses and viroids, and presents a number of thought-provoking ideas.
Download or read book Virus Diseases of Ornamental Plants written by S. K. Raj and published by Springer Nature. This book was released on 2021-11-13 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edited book elucidates the evolution of plant virus, genomic structure, diversity, plant-virus interaction, subcellular movement etc. The book reviews the biological machineries which allow the emergence of virus populations adapted by plant. The main objective of this book is the demonstration of a clear synergistic effect of plant viruses, an effect that was unexpectedly as important as applied alone. Ornamental plants are very popular and economically important worldwide. The international market of ornamentals is constantly expanding. Viruses and viroids can significantly reduce both decorative value and quality of propagated material of ornamentals. Due to the wide range of ornamental plant species and cultivars and their wide geographical distribution, the diversity of viruses that infect them is also high. The new emerging viruses are the causal agent for the economic loss of many important ornamental plants. Therefore, this book also adds value to current knowledge of virus stress response in ornamental plants and will provide the groundwork necessary for building future strategies for product enhancement. This book is of interest to teachers, researchers, capacity builders and policymakers. It can serve as additional reading material for undergraduate and graduate students of virology, agriculture and plant sciences.
Download or read book Sustainable disease management in a European context written by David B. Collinge and published by Springer Science & Business Media. This book was released on 2008-09-02 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?
Download or read book Testing Methods for Seed transmitted Viruses written by S. E. Albrechtsen and published by CABI. This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.
Download or read book Detection and Diagnostics of Plant Pathogens written by Maria Lodovica Gullino and published by Springer. This book was released on 2014-10-24 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is part of the Plant Pathology in the 21st Century Series, started in the occasion of the IX International Congress of Plant Pathology, Torino, 2008. In conjuction with the Xth International Congress of Plant Pathology, held in Beijing in August 2013. Although deriving from a Congress, the book will not have the format of traditional Proceedings, but will be organized as a resource book. It will be based on invited lectures presented at the Congress as well as by other chapters selected by the editors among offered papers. This book will cover a topic very important in the field of plant pathology, dealing with detection and diagnostics. This field of research is continuously moving forwards, due to innovation in techniques. The application of new detection and diagnostic technologies are relevant to many applied fields in agriculture. The different chapters will provide a very complete figure of the topic, from general and basic aspects to practical aspects.
Download or read book Serology and Immunochemistry of Plant Viruses written by M.H.V. Van Regenmortel and published by Academic Press. This book was released on 1982-02-28 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antigens and antigenic determinants; Antibodies; Virus purification; Preparation of Ansira and purification of antibodies; Antigen-antibody interaction; Serological techniques; Role of quaternary structure in viral antigenicity; Virus identidication; Virus classification; Immunochemical studies of plant viruses.
Download or read book An Introduction to the Diagnosis of Plant Disease written by Chelston W. D. Brathwaite and published by IICA. This book was released on 1981 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: The diagnosis of plant disease; The microscope; The autoclave; The preparation of media for fungal and bacterial growth; Detection of fungal pathogens in infected plant tissues; Detection of bacterial pathogens in infected tissues; Koch's postulates; The diagnosis of a nematode prblem; Viruses and plant virus diseases; Mycoplasma as agents of plant disease.
Download or read book Principles of Diagnostic Techniques in Plant Pathology written by R. T. V. Fox and published by Oxford University Press, USA. This book was released on 1993 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: The correct diagnosis of a plant disease is an essential prerequisite of its successful control. Diagnostic methods today include a number of traditional techniques such as direct observation or microscopy, as well as more recently developed procedures such as those based on immunological or nucleic acid analysis. This book provides a text reviewing the principles of all these techniques that will be suitable for advanced students of plant pathology who already have some basic background in the subject. The theories behind the methods are described and illustrated with numerous examples of plant diseases caused by fungi, bacteria and viruses, and the strengths and limitations of different techniques are compared. The book includes a number of colour photographs and will provide a very useful overview of this rapidly developing subject.
Download or read book Plant Virology Protocols written by Ichiro Uyeda and published by Humana. This book was released on 2016-09-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses will be an invaluable guide to researchers working in the field of plant sciences.
Download or read book Seed borne plant virus diseases written by K. Subramanya Sastry and published by Springer Science & Business Media. This book was released on 2013-01-05 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Seeds provide an efficient means in disseminating plant virus and viroid diseases. The success of modern agriculture depends on pathogen free seed with high yielding character and in turn disease management. There is a serious scientific concern about the transmission of plant viruses sexually through seed and asexually through plant propagules. The present book provides the latest information along with the total list of seed transmitted virus and viroid diseases at global level including, the yield losses, diagnostic techniques, mechanism of seed transmission, epidemiology and virus disease management aspects. Additional information is also provided on the transmission of plant virus and virus-like diseases through vegetative propagules. It is also well known that seed transmitted viruses are introduced into new countries and continents during large-scale traffic movements through infected germplasm and plant propogules. The latest diagnostic molecular techniques in different virus-host combinations along with disease management measures have been included. The book shall be a good reference source and also a text book to the research scientists, teachers, students of plant pathology, agriculture, horticulture, life sciences, green house managers, professional entrepreneurs, persons involved in quarantines and seed companies. This book has several important features of seed transmitted virus diseases and is a good informative source and thus deserves a place in almost all university libraries, seed companies and research organizations.
Download or read book Plant Virus and Viroid Diseases in the Tropics written by K. Subramanya Sastry and published by Springer Science & Business Media. This book was released on 2013-06-26 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant virus and sub-viral pathogens pose severe constraints to the production of wide range of economically important crops worldwide. The crops raised both through true seed and vegetative propagated materials are affected with number of virus and virus-like diseases. The virus may enter into plants through seed planting materials or by vectors. Once the virus is in the field, it multiplies and spreads following definite patterns depending upon the nature of the vector and agro-meteorological conditions. Disease free crops and plants are great economic and social importance in feeding the world's population. Detection of virus and sub-viral agents at initial stages of infection is critical to reduce economic losses. For nearly two decades, ELISA and its variants played a major role in large scale virus testing and also in the production of virus-free planting materials.
Download or read book Plant Cell Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
