Download or read book Techniques in Apoptosis written by Thomas G. Cotter and published by University of California Press. This book was released on 1997-01-01 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the latest techniques for researching the role of cell death in physiological and pathological mechanisms, a topic that is currently both of high interest and rapidly changing. Includes step-by-step protocols for identifying apoptotic cells from plants through fruit flies to humans; describes the common procedures for assessing apoptosis by morphology, vital-dye and DNA-fragmentation assays, and plasma membrane changes; and explores a variety of systems used to study the biochemistry and genetic regulation of apoptosis. The flat wire binding allows the pages to lie flat on the laboratory bench. Distributed in the US by Ashgate. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book Apoptosis written by P. Erhard and published by Humana Press. This book was released on 2009-07-15 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.
Download or read book Apoptosis Methods and Protocols written by Hugh J. M. Brady and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most fundamental question facing each and every cell within an org- ism is to survive or to die. Cell death is required for normal function; some estimates suggest that as many as one million cells undergo cell death every second in the adult human body. Almost all cells undergoing physiological, or programmed, cell death, independent of cell type, manifest a stereotypic p- tern of morphological changes termed apoptosis. Typically, apoptotic cells d- play shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. The integrity of the cell membrane is not lost during apoptosis and so avoids eliciting the inflammatory response that would have been caused by the spillage of the cell’s contents. This is quite in contrast to the loss of cell contents typical of necrosis. The caspases, the family of intracellular cysteine proteases associated with apoptosis, are responsible for the stereotypical m- phological changes. Caspases cleave various substrate proteins that act on DNA fragmentation, nuclear envelope integrity, the cytoskeleton, and cell volume regulation. Apoptotic cells are cleared in vivo by the process of phagocytosis, in which specific “phagocytes” move to the site of apoptosis, engulf the dying cells and digest them. Apoptosis has a central role in many physiological processes, for example, in the immune system. Autoreactive cells are deleted via apoptosis to prevent autoimmunity. At the end of an immune response, activated lymphocytes are removed to maintain homeostasis within the immune system.
Download or read book Apoptosis and Cancer written by Gil Mor and published by Springer Science & Business Media. This book was released on 2007-10-11 with total page 271 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
Download or read book Apoptosis Techniques and Protocols written by Andréa C. LeBlanc and published by Springer Science & Business Media. This book was released on 2008-02-06 with total page 259 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis.
Download or read book Apoptosis Methods in Pharmacology and Toxicology written by Myrtle A. Davis and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Programmed Cell Death written by Hamsa Puthalakath and published by Humana. This book was released on 2016-04-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains protocols specifically designed for studying programmed cell death, and also discusses recent advances in techniques that span broader areas of biology that have been recently used or that have potential to be incorporated into cell death research. The protocols are mostly described in the context of mammalian systems, but also cover other systems such as plants, Drosophila, and yeast. Programmed Cell Death: Methods and Protocols is comprised of 20 chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9 describe methods for studying apoptosis associated with various pathologies in different organs including the lymphoid compartment, intestinal epithelium, granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques for studying apoptosis in non-mammalian systems; Chapters 14-16 cover biochemical and biophysical methods for studying Bcl-2 family protein dynamics and protein-protein interactions during apoptosis; and the last four chapters explore protocols that are useful not only in apoptosis research but in wider areas of biological research, such as genome editing, inducible transgenes, and proteomics. Written in the highly successful Methods in Molecular Biology aeries format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocol, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Programmed Cell Death: Methods and Protocols is a comprehensive and valuable resource for researchers, ranging from beginner to expert, in their studies on programmed cell death.
Download or read book Cell Death written by Douglas R. Green and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A million cells in our bodies die every second--they commit suicide by activating a process called apoptosis or other forms of programmed cell death. These mechanisms are essential for survival of the body as a whole and play critical roles in various developmental processes, the immune system, and cancer. In this second edition of Douglas Green's essential book on cell death, Green retains the bottom-up approach of the first edition, starting with the enzymes that carry out the execution (caspases) and their cellular targets before examining the machinery that connects them to signals that cause cell death. He also describes the roles of cell death in development, neuronal selection, and the development of self-tolerance in the immune system, as well as how the body uses cell death to defend against cancer. The new edition is fully updated to cover the many recent advances in our understanding of the death machinery and signals that control cell death. These include the mechanisms regulating necroptosis, mitophagy, and newly identified processes, such as ferroptosis. The book will thus be of great interest to researchers actively working in the field, as well as biologists and undergraduates encountering the topic for the first time.
Download or read book Apoptosis and Cancer written by Gil Mor and published by Humana Press. This book was released on 2007-10-11 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Apoptosis and Cancer is to describe the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. The protocols described will aid both the academic laboratory interested in further characterizing the mechanisms of apoptosis, as well as the industry laboratory, aimed at identifying new target molecules or screening for new compounds with potential clinical use.
Download or read book Proteases in Apoptosis Pathways Protocols and Translational Advances written by Kakoli Bose and published by Springer. This book was released on 2015-08-06 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the proteases involved in programmed cell death. It presents a focused yet extensive discussion on proteolytic enzymes such as caspases, HtrAs, granzymes, calpains and cathepsins as well as laboratory protocols related to enzymology and apoptosis. Mouse model systems and non-invasive imaging techniques in apoptosis-related diseases such as cancer and neurodegeneration are also covered in this book. While slowly unravelling the complexities of apoptosis in chapter one, the next three chapters individually elaborate on different classes of proteases that play key roles in the initiation, progression and execution of programmed cell death. The last two chapters complete this discussion by describing different laboratory methodologies and therapeutic advances involving apoptotic proteases. Protocols portraying in vitro and ex vivo colorimetric and fluorescence-based enzyme kinetic studies as well as cell death assays are explained in the fifth chapter. Preclinical in vivo models and non-invasive imaging in apoptosis to understand the complexities of disease progression and their contribution toward therapeutics is recounted in the last chapter. The book spans topics related to both fundamental and applied biology. It would therefore be equally appealing and informative to scientists working in the field of apoptosis and those who are investigating mechanisms of proteases and enzymes in general. The protocols would certainly benefit both graduate and undergraduate students working in the related fields and provide useful leads for drug design to translational biologists involved in neurodegeneration and cancer research.
Download or read book Apoptosis Detection and Assay Methods written by Li Zhu and published by Eaton Publishing Company/Biotechniques Books. This book was released on 1998 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.
Download or read book Apoptosis in Neurobiology written by Yusuf A. Hannun and published by CRC Press. This book was released on 1998-12-24 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid growth of the study of apoptosis-mechanism-driven, regulated cell death-has created an urgent need for reliable documentation of the different approaches to and methods of studying the various aspects of the field. Apoptosis in Neurobiology is an important resource for researchers in this emerging frontier of biomedical study. This volume allows the uninitiated neuroscientist intellectual and practical access to the study of apoptosis, with special consideration to the nervous system. The first section concentrates on conceptual approaches to the study of apoptosis in neurobiology and its significance to the nervous system. The second section provides a user-friendly approach to methods and techniques in the study of apoptosis as applied to neurobiology.
Download or read book Clinical Perspectives and Targeted Therapies in Apoptosis written by Rupinder K. Sodhi and published by Academic Press. This book was released on 2020-10-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical Perspectives and Targeted Therapies in Apoptosis: Drug Discovery, Drug Delivery, and Disease Prevention provides comprehensive coverage, from basic cell biology, to modern assessment techniques for apoptosis in all major disease areas. Chapters provide an introduction to the fundamentals of cell biology, biochemical mechanisms, and the pathophysiological consequences of apoptosis. In addition, the book covers the tools and techniques used to quantify apoptosis and the significance of apoptosis in drug discovery, drug delivery, and its applications in disease prevention. Finally, the book provides a comprehensive compilation of the apoptosis targeting drugs that recently underwent clinical trials. This combination of fundamentals, along with applications in drug discovery, drug delivery, and clinical research make this book a useful resource for those in both academia and industry who are engaged in pharmaceutical, biomedical and biotechnology research.
Download or read book Mammalian Cell Viability written by Martin J. Stoddart and published by Humana Press. This book was released on 2011-04-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
Download or read book Apoptosis Methods in Toxicology written by Perpetua M. Muganda and published by Methods in Pharmacology and To. This book was released on 2018-04-28 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt:
