Download or read book Systematic Analysis of Cell Size Control in the Budding Yeast Saccharomyces Cerevisiae written by Michael Alexander Cook and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of Cell Size Control in the Yeast Saccharomyces Cerevisiae microform Isolation and Characterization of WHI3 a Dose dependent Inhibitor of Start written by Robert Stuart Nash and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 1993 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Biology of the Cell Cycle written by J. M. Mitchison and published by CUP Archive. This book was released on 1971-11-30 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Correspondence Analysis Handbook written by Benzecri and published by CRC Press. This book was released on 1992-01-22 with total page 684 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical reference/text presents a complete introduction to the practice of data analysis - clarifying the geometrical language used, explaining the formulae, reviewing linear algebra and multidimensional Euclidean geometry, and including proofs of results. It is intended as either a self-study guide for professionals involved in experimental
Download or read book Analysis of Cell Size Control in the Yeast Saccharomyces Cerevisiae written by Robert Stuart Nash and published by . This book was released on 1993 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Yeast Genetics written by Jeffrey S. Smith and published by Humana Press. This book was released on 2014-09-12 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Download or read book Stress Activated Protein Kinases written by Francesc Posas and published by Springer Science & Business Media. This book was released on 2008-01-24 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book leading researchers in the field discuss the state-of-the-art of many aspects of SAPK signaling in various systems from yeast to mammals. These include various chapters on regulatory mechanisms as well as the contribution of the SAPK signaling pathways to processes such as gene expression, metabolism, cell cycle regulation, immune responses and tumorigenesis. Written by international experts, the book will appeal to cell biologists and biochemists.
Download or read book The Evolution of Senescence in the Tree of Life written by Richard P. Shefferson and published by Cambridge University Press. This book was released on 2017-02-23 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The existing theories on the evolution of senescence assume that senescence is inevitable in all organisms. However, recent studies have shown that this is not necessarily true. A better understanding of senescence and its underlying mechanisms could have far-reaching consequences for conservation and eco-evolutionary research. This book is the first to offer interdisciplinary perspectives on the evolution of senescence in many species, setting the stage for further developments. It brings together new insights from a wide range of scientific fields and cutting-edge research done on a multitude of different animals (including humans), plants and microbes, giving the reader a complete overview of recent developments and of the controversies currently surrounding the topic. Written by specialists from a variety of disciplines, this book is a valuable source of information for students and researchers interested in ageing and life history traits and populations.
Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Download or read book Cell Growth written by Michael N. Hall and published by CSHL Press. This book was released on 2004 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent breakthroughs in the field of cell growth, particularly in the control of cell size, are reviewed by experts in the three major divisions of the field: growth of individual cells, growth of organs, and regulation of cell growth in the contexts of development and cell division. This book is an introductory overview of the field and should be adaptable as a textbook.
Download or read book Yeast written by Horst Feldmann and published by John Wiley & Sons. This book was released on 2012-09-06 with total page 1 pages. Available in PDF, EPUB and Kindle. Book excerpt: Finally, a stand-alone, all-inclusive textbook on yeast biology. Based on the feedback resulting from his highly successful monograph, Horst Feldmann has totally rewritten he contents to produce a comprehensive, student-friendly textbook on the topic. The scope has been widened, with almost double the content so as to include all aspects of yeast biology, from genetics via cell biology right up to biotechnology applications. The cell and molecular biology sections have been vastly expanded, while information on other yeast species has been added, with contributions from additional authors. Naturally, the illustrations are in full color throughout, and the book is backed by a complimentary website. The resulting textbook caters to the needs of an increasing number of students in biomedical research, cell and molecular biology, microbiology and biotechnology who end up using yeast as an important tool or model organism.
Download or read book Cell cycle checkpoint control in budding yeast saccharomyces cerevisiae PHD written by Gretchen Louise Kiser and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mitotic Exit Network written by Fernando Monje-Casas and published by Humana. This book was released on 2016-11-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.
Download or read book Regulation of Cell Size and the Size Dependence of Gene Expression written by Devon Chandler-Brown and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume is a fundamental morphological feature of cells, influencing a wide variety of cellular processes. Because it is coupled to so many processes, cells employ regulatory mechanisms to ensure cells exhibit a limited range of sizes. Recent work in budding yeast has shown that a key cell cycle regulator, the G1/S transcriptional inhibitor Whi5, is synthesized independent of cell size. The dilution of Whi5 in larger cells links cell size to G1/S cell cycle progression. However, it has also been shown that growth over the full cell cycle does not depend on cell size at birth, termed an "adder". It has been proposed that this observation suggests that cell size is controlled over the course of the full cell cycle, leading to an apparent contradiction. Here we show that cell size control occurs independently in different parts of the cell cycle and does not reflect a molecular mechanism measuring growth during the full cell cycle. Consistent with previous results, we find that cell size sets the rate of entry into the cell cycle during the pre-Start period. We also identify the key parameters predicting the rate of entry into cytokinesis at the end of the post-Start period. We use these parameters to build a phenomenological cell cycle model that recapitulates observations of growth and size distributions for cells without explicit coupling between cell cycle phases. Our model predicts that changes to the rate of progression through either phase of the cell cycle should disrupt the adder behavior and we show that mutants in genes controlling G1/S size control breaks the adder. The rate of passage through Start depends on volume, which is thought to depend on the size-independent expression of Whi5. This type of gene expression scaling is unusual because although cells of a given type may span a range of sizes, most proteins and RNA are maintained at constant, size independent, concentrations, rather than amounts. This ensures that biochemical reactions proceed independently of cell size. The identification of WHI5, whose gene product differs from this pattern, raises two fundamental questions: (1) Are there additional genes whose synthesis is decoupled from cell volume? (2) If most gene expression is proportional to cell size, what molecular mechanism promotes cell-size-independent gene expression? To address these questions, we analyzed flow cytometry data collected using the yeast GFP-fusion library. We identified approximately 200 genes whose expression is not proportional to cell volume. Gene ontology analysis revealed that non-scaling genes are enriched for genes with roles in DNA-templated processes and membrane transport. This suggests that cells employ differential protein synthesis to coordinate protein requirements with the scaling properties of cellular structures. Membranes are expected to scale as size2/3 and DNA content is independent of size. To understand the mechanisms that underlie size-independent gene expression, we used transcriptional reporters of non-scaling genes, including WHI5, and determined that cell-size-independent regulation of some genes is due to non-scaling transcription rates. Targeted analysis of the WHI5 promoter showed that the region between 1000 bases and 550 bases upstream of the translation start site are required for cell-size-independent gene expression. This suggests there is a molecular element within this region required for non-scaling gene expression. Finally, we identify a partitioning mechanism ensuring proteins are partitioned in dividing cells in amounts that are independent of asymmetric sizes of the mother and daughter cells. Tight chromatin association ensures that proteins are segregated in equal amounts despite asymmetric division. Consistent with this model, while Whi5 is normally partitioned in equal amounts, a Whi5 protein that lacks the domain required for association with transcription factors is partitioned in proportion to the mother-daughter cell size ratio. Taken together, our work demonstrates a functional role for differential size-dependency of protein synthesis and gives insights into the underlying molecular mechanism(s).
Download or read book A Systematic Cell Size Screen in Saccharomyces Cerevisiae and Characterization of the Start Inhibitor Whi5 written by Joy Luz Nishikawa and published by . This book was released on 2008 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell size homeostasis requires the precise coordination of growth and division. In the budding yeast Saccharomyces cerevisiae, these processes are coordinated by a critical cell size threshold, which is exerted in late G1 phase immediately prior to Start. At Start, cells irrevocably commit to a subsequent round of cell division. The construction of a complete set of & sim;6000 yeast strains deleted for every open reading frame allowed us to systematically identify abnormally large (lge) or small (whi) mutants with potential roles in cell size homeostasis. This screen identified over 40 novel components involved in setting the critical cell size threshold at Start. In particular, we found that ribosome biogenesis plays a crucial role in cell size determination; this pathway appears to be evolutionarily conserved. From this screen I further characterized the novel Start repressor Whi5. Entry into S-phase is driven by two heterodimeric transcription factors, SBF and MBF; Whi5 represses S-phase entry by directly binding to SBF and MBF, inhibiting their activation. I found that Whi5 is phosphorylated by all forms of cyclin-Cdc28 activity and that phosphorylation of the Whi5-SBF complex in late G1 phase results in the elimination of Whi5 activity by two mechanisms: Whi5 dissociation from SBF/MBF, and subsequently, nuclear export of Whi5 by the exportin Msn5. Efficient phospho-dependent export of Whi5 by Msn5 depends upon a threshold level of phosphorylation. Upon the release of Whi5, the RNA PolII holoenzyme and general transcription factors are recruited to and activate SBF/MBF-regulated promoters. My data demonstrate that Whi5 links cyclin-Cdc28 activity to S-phase entry. Upon the elimination of Cdc28 activity in late anaphase, Whi5 is dephosphorylated by Cdc14, allowing for its import by the importin Kap121 and subsequent G1-phase repression of SBF/MBF. Whi5 is functionally analogous to members of the retinoblastoma tumor suppressor protein (pRB) family in mammalian cells. Like Whi5, pRB-family members repress E2F transcription factors until the reactivation of CDK activity, and phosphorylation of pRB-family members by cyclin-CDK complexes in late G1 phase eliminates pRB-mediated repression. Thus, the identification of Whi5 unifies G1-S control in eukaryotes.
Download or read book Cell Cycle Oscillators written by Amanda S. Coutts and published by Humana. This book was released on 2015-08-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.
