Download or read book Subcellular Fractionation written by John M. Graham and published by Oxford University Press. This book was released on 1997 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the fast changing world of cell biology it is often required that particular membranes or subcellular organisms be isolated from the structure for investigative purposes. This book shows how to do this in a practical, hands-on way.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Subcellular Fractionation written by Paul R. Pryor and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Download or read book Subcellular Fractionation written by Paul R. Pryor and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Download or read book Subcellular Fractionation written by J. M. Graham and published by IRL Press. This book was released on 1997-01-30 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
Download or read book Subcellular Components written by G. D. Birnie and published by Elsevier. This book was released on 2014-05-20 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subcellular Components: Preparation and Fractionation talks about cells and particles' components, including their preparation and fractionation. The book includes theories and answers to questions that are relevant to the study. The first chapter of the book details various facts about homogenization of mammalian cells. This chapter presents the results of studies on solid tissues and single-cell suspensions; the author then offers his conclusion of the study. The next two chapters highlight the methods on isolating nuclei, including the guides for standard assessment and the procedure of isolation, along with analysis of nuclei biochemical properties. The main topics in Chapter 4 are mitochondria from animal tissues and yeasts; this chapter also discusses the preparation for a rat-liver, blowfly flight-muscle, yeast, and brain mitochondria. The chapter that follows widely talks about lysosomes, including its historical background, centrifugal method, and related topics. In the next several chapters, the topics covered include purification, isolation, preparation, and separation of cells including plasma-membrane, polysomes, ribosomes, microsomes, and microvilli. The book serves as a great reference for undergraduates and postgraduates in the field, as it contains a thorough discussion of various relevant studies.
Download or read book Subcellular Fractionation written by M. Graham and published by . This book was released on 1997 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.
Download or read book Subcellular Fractions Advances in Research and Application 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subcellular Fractions: Advances in Research and Application: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Subcellular Fractions. The editors have built Subcellular Fractions: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Subcellular Fractions in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Subcellular Fractions: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Adipose Tissue Protocols written by Gérard Ailhaud and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.
Download or read book Subcellular Proteomics written by Eric Bertrand and published by Springer Science & Business Media. This book was released on 2007-08-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
Download or read book Subcellular Fractionation and Centrifugation written by K. R. Smith and published by . This book was released on 1988 with total page 58 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Separation of Cells and Subcellular Elements written by H. Peeters and published by Elsevier. This book was released on 2017-08-02 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients. The book can provide useful information to biochemists, cytologists, students, and researchers.
Download or read book Subcellular Fractionation written by David LLoyd and published by . This book was released on 1979 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Cell Death Mechanisms written by Ayesha B. Alvero and published by . This book was released on 2021 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides detailed protocols for the performance, analysis, and troubleshooting of in vitro and in vivo experiments related to programmed cell death. Chapters compile conventional techniques such as western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Detection of Cell Death Mechanisms: Methods and Protocols aims to ensure seamless execution of protocols on specific cell death type.
Download or read book Bacterial Protein Secretion Systems written by Laure Journet and published by Humana Press. This book was released on 2017-07-01 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
Download or read book Subcellular Fractions Advances in Research and Application 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subcellular Fractions—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Cell-Free System. The editors have built Subcellular Fractions—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cell-Free System in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Subcellular Fractions—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Download or read book Eukaryotic Cell Function and Growth written by Jacques E. Dumont and published by Springer. This book was released on 1976-08 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
