Download or read book Studies on Cell Division Protein FtsZ in Escherichia Coli written by Jan Louis Albert Voskuil and published by . This book was released on 1995 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Prokaryotic Cytoskeletons written by Jan Löwe and published by Springer. This book was released on 2017-05-11 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the structures and functions of active protein filaments, found in bacteria and archaea, and now known to perform crucial roles in cell division and intra-cellular motility, as well as being essential for controlling cell shape and growth. These roles are possible because the cytoskeletal and cytomotive filaments provide long range order from small subunits. Studies of these filaments are therefore of central importance to understanding prokaryotic cell biology. The wide variation in subunit and polymer structure and its relationship with the range of functions also provide important insights into cell evolution, including the emergence of eukaryotic cells. Individual chapters, written by leading researchers, review the great advances made in the past 20-25 years, and still ongoing, to discover the architectures, dynamics and roles of filaments found in relevant model organisms. Others describe one of the families of dynamic filaments found in many species. The most common types of filament are deeply related to eukaryotic cytoskeletal proteins, notably actin and tubulin that polymerise and depolymerise under the control of nucleotide hydrolysis. Related systems are found to perform a variety of roles, depending on the organisms. Surprisingly, prokaryotes all lack the molecular motors associated with eukaryotic F-actin and microtubules. Archaea, but not bacteria, also have active filaments related to the eukaryotic ESCRT system. Non-dynamic fibres, including intermediate filament-like structures, are known to occur in some bacteria.. Details of known filament structures are discussed and related to what has been established about their molecular mechanisms, including current controversies. The final chapter covers the use of some of these dynamic filaments in Systems Biology research. The level of information in all chapters is suitable both for active researchers and for advanced students in courses involving bacterial or archaeal physiology, molecular microbiology, structural cell biology, molecular motility or evolution. Chapter 3 of this book is open access under a CC BY 4.0 license.

Download or read book Interaction of the Cell Division Protein FtsZ with the Escherichia Coli Inner Membrane written by Cecile-Marie Koppelman and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Studies on the Escherichia Coli Cell Division Gene FtsZ written by John E. Ward and published by . This book was released on 1985 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Bacterial Cell Coupling between Growth Nucleoid Replication Cell Division and Shape Volume 2 written by Ariel Amir and published by Frontiers Media SA. This book was released on 2019-11-14 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 1st volume of our Research Topic "The Bacterial Cell: Coupling between Growth, Nucleoid Replication, Cell Division and Shape” was published as an eBook in May 2016 (see: http://journal.frontiersin.org/researchtopic/2905/the-bacterial-cell-coupling-between-growth-nucleoid-replication-cell-division-and-shape). As a sign of growing interest to the topic, two workshops followed the same year: "Stochasticity in the Cell Cycle" in Jerusalem (Israel) by the Hebrew University’s Institute of Advanced Studies and EMBO's "Cell Size Regulation" in Joachimsthal (Germany). From the time of launching the first edition, several new groups have entered the field, and many established groups have made significant advances using state-of-the-art microscopy and microfluidics. Combining these approaches with the techniques pioneered by quantitative microbiologists decades ago, these approaches have provided remarkable amounts of numerical data. Most of these data needed yet to be put into a broader theoretical perspective. Moreover, the molecular mechanisms governing coordination and progression of the main bacterial cell cycle processes have remained largely unknown. These outstanding fundamental questions and the growing interest to the field motivated us to launch the next volume titled “The Bacterial Cell: Coupling between Growth, Nucleoid Replication, Cell Division, and Shape, Volume 2” shortly after completion of the first edition in October 2016. The issue contains 17 contributions from a diverse array of scientists whose field of study spans microbiology, biochemistry, genetics, experimental and theoretical biophysics. The specific questions addressed in the issue include: What triggers initiation of chromosome replication? How is cell division coordinated with replication both spatially and temporally? How is cell size controlled and linked to the rate of mass growth? What role plays physical organization of the chromosomes in their segregation and in regulation of cell division? The publications covering these questions are divided into three topical areas: 1) Cell Cycle Regulation, 2) Growth and Division, and 3) Nucleoid Structure and Replication. New ideas and techniques put forward in these articles bring us closer to understand these fundamental cellular processes, but the quest to resolve them is far from being complete. Plans for the next edition are under way along with further meetings and workshops, e.g., an EMBO Workshop on Bacterial cell biophysics: DNA replication, growth, division, size and shape in Ein Gedi (Israel), May 2020. We hope that via such interdisciplinary exchange of ideas we will come closer to answering the above-mentioned complex and multifaceted questions.

Download or read book Studies on Escherichia Coli Cell Division written by Haichao Wang and published by . This book was released on 1992 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Cell Division Studies of Escherichia Coli written by Younghae Chong Chon and published by . This book was released on 1988 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bioresources and Bioprocess in Biotechnology written by Shiburaj Sugathan and published by Springer. This book was released on 2017-05-26 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compilation of detailed articles on various products and services that can be derived from bioresources through bioprocess. It offers in-depth discussions and case studies on commercially and therapeutically important enzymes, antimicrobials, anti-cancer molecules and anti-inflammatory substances. It also includes a separate section on emerging trends in bioactive substances research. This unique book is a valuable source of information for biotechnologists and bioprocess experts as well as academics and researchers who are actively involved in product and process development.

Download or read book Temperature sensitive FtsZ Alleles that Modulate the Rod to Sphere Morphogenesis in Escherichia Coli written by Ziad Walid El-Hajj and published by . This book was released on 2005 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The normally rod-shaped bacterium Escherichia coli K-12 divides by elongating and then forming a septum at midcell, which will result in the format ion of two daughter cells. Septation is driven by the polymerization of the essential d ivision protein FtsZ into a structure called the Z-ring, and the subsequent recruitment of other cell division proteins to form the divisome. Temperature-sensitive (Ts) ftsZ mut ants can divide as rods at 30 degreesC but filament and lyse at 42 degreesC, and are ther efore used as tools to study the role of FtsZ in cell division. The rod shape of E. coli is maintained by the pbpA gene product Penicillin-Binding Protein 2 (PBP2), and upon inactivation of pbpA the cells become spherical, fail to divide, and eventually die. Simultaneous overexpression of the essential cell division genes ftsQ, ftsA and ftsZ from the lowcopy- number plasmid pBS58 restores division in spherical cells on LB agar 30 degreesC and 37 degreesC but not 42 degreesC. Several temperature-sensitive (Ts) ftsZ mutants were tested for their ability to support division in spheres, with the mutation being either on the chromosome, o n a pBS58 derivative, or both. At the permissive temperature of 30degreesC, ftsZ84 s upported division in all combinations. On the other hand, ftsZ972, ftsZ2066, ftsZ2863, fts Z6460 and ftsZ9124 supported division and viability in spherical cells only when prese nt in combination with wild-type ftsZ. Furthermore, an ftsZ26 mutant carrying pBS58 co uld divide more efficiently as a sphere than a rod. Overexpression of ftsWin additio n to ftsQAZ recovered spherical division in ftsZ972 but had no effect on the other mu tants. Surprisingly, the ftsZ26, ftsZ84, ftsZ2863 and ftsZ6460 mutations in combination with wild-type ftsZ supported division in spheres at the non-permissive temperature. These data demonstrate that in addition to conferring temperature-sensitivity, some ft sZ(Ts) mutants impair cell division lethally at the permissive temperature in spherical but not rod-shaped E. coli, and a few of these as well as other mutants suppress the dea th of spherical cells at the non-permissive temperature. Sphere-dependent phenotypes o f ftsZ(Ts) alleles are likely to reflect subtle effects on key events during cell division, including FtsZ polymerization and/or its interactions with other cell division p ...

Download or read book Studies on Cell Division and Shape in Escherichia Coli written by Yevgeny A. Nikolaichik and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on Cell Division and Shape in Escherichia Coli written by Yevgeny A. Nikolaichik and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structural and Functional Analysis of the E Coli Cell Division Proteins FtsL and FtsB written by Samuel James Craven and published by . This book was released on 2020 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell division in bacteria is mediated by a multiprotein machine called the divisome, which is responsible both for directing constrictive force and remodeling the cell wall into a septum at midcell. In Escherichia coli, an essential component of the divisome is the FtsLB complex, which is necessary for recruiting other proteins to the division site and is involved with triggering the final constriction event. The structural arrangement, specific functions, and interaction network of FtsLB are still poorly understood, and improved insights into the roles this essential complex plays would lead to a more complete understanding of cell division as a whole. In this dissertation, I will discuss a series of investigations from myself and collaborators that address how different structural aspects of FtsLB relate to its function. First, we establish via FRET that FtsLB forms a likely 2:2 heterotetramer and use a combination of co-evolutionary analysis and computational modeling to produce a structural model for the complex. These results suggest that the coiled coil of FtsLB is marginally stable due to an abundance of non-hydrophobic interfacial residues. To address a functional role for these residues, we use in vivo complementation experiments and incorporate our results into an alternative structural model. We also provide in vivo evidence that FtsB contains a flexible linker region, whereas FtsL is a continuous, rigid helix. Finally, we identify potential binding sites between FtsL and the essential cell wall synthase FtsW using both in vivo complementation and in vitro binding assays. Together, these studies suggest that distinct structural features of FtsLB - namely its tetrameric arrangement, marginally stable coiled coil, and site of interaction with FtsW - are critical components of divisome regulation. This has important implications for the study of bacterial cell division, as improving our understanding of such a key regulatory component will ultimately lead to a better comprehension of the divisome as a whole.

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Escherichia Coli and Salmonella written by Frederick C. Neidhardt and published by . This book was released on 1996 with total page 2822 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the long–awaited second edition of an invaluable classic! Escherichia coli occupies a central role in contemporary molecular biology. It is the unicellular organism about which most is known – all molecular and cellular biologists will want a copy of this book. In 154 chapters, 250 expert authors and editors present the state of the art. Completely rewritten and restructured, the second edition offers a whole new approach to the subject.

Download or read book Multiple Action Based Design Approaches to Antibacterials written by John Bremner and published by Springer Nature. This book was released on 2021-04-05 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers intentional design aspects for combinations of drugs, single-molecule hybrids with potential or actual multiple actions, pro-drugs which could yield multiple activity outcomes, and future possibilities. The approach of the book is interdisciplinary, and it provides greater understanding of the complex interplay of factors involved in the medicinal chemistry design and laboratory development of multiply active antibacterials. The scope of the book appeals to readers who are researching in the field of antibacterials using the approach of medicinal chemistry design and drug development.

Download or read book Guidebook to the Cytoskeletal and Motor Proteins written by Thomas Kreis and published by Sambrook and Tooze Publication at Oxford University Press. This book was released on 1999 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition, many new and important discoveries have been made in this expanding area of research. This updated edition details all that is currently known about the proteins involved in the microfilament, microtubule and intermediate filament systems in cells and their roles intension resistance and motor function. Biochemists will find this book particularly useful as will physiologists and biologists in fields in which these proteins are involved, such as neuromuscular research.

Download or read book Molecules in Time and Space written by Miguel Vicente and published by Springer Science & Business Media. This book was released on 2004-07-02 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the last decade a wealth of new data has arisen from the use of new fluorescent labelling techniques and the sequencing of whole microbial genomes. One important conclusion from these data is that bacterial cells are much more structured than previously thought. The wall and the outer membrane contain topological domains, some proteins localize or move in specific patterns inside the cells, and some genes appear clustered in the chromosome and form conserved evolutionary units. Many of these structures are related to the cell cycle and to the process of cell morphogenesis, two processes that are themselves related to each other. From these observations the dcw gene cluster appears as a phylogenetic trait that is mainly conserved in bacilli. Molecules in Time and Space reviews the data on the formation of subcellular patterns or structures in bacteria, presents observations and hypotheses on the establishment and the maintenance of cell shape, and on the organization of genetic information in the chromosome.