Download or read book Studies of the Genetic Regulation of the Thermomonospora Cellulase Complex written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this project are to determine the molecular mechanisms regulating cellulose synthesis in the soil bacterium Thermomonosporafusca and to determine the molecular mechanism by which T.fusca cellulases degrade crystalline cellulose. We have determined a structure for the T.fusca E2 catalytic subunit (E230 by x-ray crystallography. This structure is quite similar to that of T.reesei CBHU but there are a number of differences. One is that the E2 active site is in a cleft while that of CBHII is in a tunnel. This is an expected result since E2 is an endocellulase. Large amounts of homogenous E5 catalytic subunit have been prepared and attempts to crystallize it are underway. Crystals of E2-30 were soaked in cellobiose and modified crystals detracted well, however difference Fourier analysis showed many changes, so that we could not localize cellobiose in the 3-D structure of E2-30. This implies that binding of cellobiose causes a significant change in the structure of E2-30. The stereochemistry of the cleavage catalyzed by E{sub l}, E2 and E5 was determined in collaboration with Dr. Stephen Withers and E1 and 2 inverted the glycoside linkage while E5 does not. The entire E{sub l} and E4 genes have been induced into Streptomyces lividans where they are expressed at a high level and the E{sub l} and E4 are completely secreted into the medium. Studies on the synergism between the exocellulase E3 and the endocellulases E2 or E5 show that both exo and endocellulase activities are stimulated when they are assayed together.

Download or read book Studies of the Genetic Regulation of the Thermomonospora Cellulase Complex Progress Report June 1 1990 January 10 1992 written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this project are to determine the molecular mechanisms regulating cellulose synthesis in the soil bacterium Thermomonosporafusca and to determine the molecular mechanism by which T.fusca cellulases degrade crystalline cellulose. We have determined a structure for the T.fusca E2 catalytic subunit (E230 by x-ray crystallography. This structure is quite similar to that of T.reesei CBHU but there are a number of differences. One is that the E2 active site is in a cleft while that of CBHII is in a tunnel. This is an expected result since E2 is an endocellulase. Large amounts of homogenous E5 catalytic subunit have been prepared and attempts to crystallize it are underway. Crystals of E2-30 were soaked in cellobiose and modified crystals detracted well, however difference Fourier analysis showed many changes, so that we could not localize cellobiose in the 3-D structure of E2-30. This implies that binding of cellobiose causes a significant change in the structure of E2-30. The stereochemistry of the cleavage catalyzed by E{sub l}, E2 and E5 was determined in collaboration with Dr. Stephen Withers and E1 and 2 inverted the glycoside linkage while E5 does not. The entire E{sub l} and E4 genes have been induced into Streptomyces lividans where they are expressed at a high level and the E{sub l} and E4 are completely secreted into the medium. Studies on the synergism between the exocellulase E3 and the endocellulases E2 or E5 show that both exo and endocellulase activities are stimulated when they are assayed together.

Download or read book Fiscal Year 1986 Department of Energy Authorization basic Research Programs written by United States. Congress. House. Committee on Science and Technology. Subcommittee on Energy Development and Applications and published by . This book was released on 1985 with total page 1160 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy and Water Development Appropriations for 1995 Dept of Energy Federal Energy Regulatory Commission written by United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development and published by . This book was released on 1994 with total page 2018 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy Research Abstracts written by and published by . This book was released on 1993 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy and Water Development Appropriations for 1995 written by United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development and published by . This book was released on 1994 with total page 2094 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy and Water Development Appropriations for 1998 Secretary of Energy written by United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development and published by . This book was released on 1997 with total page 1300 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Energy and Water Development Appropriations for 1998 written by United States. Congress. House. Committee on Appropriations. Subcommittee on Energy and Water Development and published by . This book was released on 1997 with total page 1918 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book International Biosystems written by and published by . This book was released on 1989 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Cellulase Genes in Thermomonospora Fusca written by Ershen Lin and published by . This book was released on 1987 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Enzymatic Plastic Degradation written by and published by Academic Press. This book was released on 2021-02-10 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic Plastic Degradation, Volume 648 in the Methods in Enzymology series, continues the legacy of this premier serial with chapters authored by leaders in the field. Chapters in this latest release include Evaluating plastic pollution and environmental degradation, Assessment methods for microplastic pollution in the oceans and fresh water, Exploring microbial consortia from various environments for plastic degradation, Characterization of filamentous fungi for attack on synthetic polymers via biological Fenton chemistry, Synthesis of radioactive-labeled nanoplastics for assaying the environmental (microbial) PS degradation, Exploring metagenome for plastic degrading enzymes, Cutinases from thermophilic bacteria (actinomycetes): from identification to functional and structural characterization, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Covers the latest research and technologies in enzymatic plastic degradation

Download or read book Regulation of Cellulase Synthesis in Thermomonospora Fusca and Cloning Sequencing and Expression of an Alkaline Serine Protease Gene in Thermomonospora Fusca written by Guifang Lao and published by . This book was released on 1996 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Government reports annual index written by and published by . This book was released on 199? with total page 1190 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biosynthesis and Biodegradation of Cellulose written by Candace H. Haigler and published by CRC Press. This book was released on 1990-12-20 with total page 713 pages. Available in PDF, EPUB and Kindle. Book excerpt: A gathering of articles bringing together knowledge of both the synthesis and degradation of a pervasive biological substance, cellulose. Topics include native cellulose; particle rosettes and terminal globules; microfibril biogenesis; synthesis in Acetobacter xylinum ; biodegradation measurement; e

Download or read book Microbial Resource Technologies for Sustainable Development written by Joginder Singh and published by Elsevier. This book was released on 2022-06-25 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial Resource Technologies for Sustainable Development describes the production and uses of microbial cells and metabolites and reviews the microbial resource technologies associated with providing sustainable solutions options in future endeavors in managing microbial resources. The book includes the recent development and scientific demonstrations of microbial technologies in the relationship between microbes and the environment, focusing on its effective resource management to achieve agricultural and environmental sustainability. Topics covered in the book include recent applications and exploration of the development of Marine Microbial Technologies for marine resources, soil microbes as biopolymers for enhancing mechanical properties of soil, and more. Other topics discussed include rhizosphere microbiome for enhancement of the cereal crops, endophytic fungal communities in crops grown under different farming systems, microbiota of termite for lignocellulose breakdown, microbial consortium technologies to produce biomethane from waste effluents, microbial technologies for sustainable food additives production, biological synthesis of the nanoparticles, fungal cellulases, and efficient biofuel and acetic acid production using waste residues with an emphasis on the commercial exploitation of such microbial technologies. Discusses the enhancement of plant production through growth-promoting microbes Considers microbial degradation and environmental management of wastes Covers microbial applications in biofuel and bioenergy production Explores plant-microbe interactions for removal of heavy metals from contaminated areas Explains engineered microorganisms for effective bioremediation Describes potential indigenous/effective microbes for food and industrial treatment processes Presents research on microbes for sustainable agricultural practices

Download or read book Recombinant Microbes for Industrial and Agricultural Applications written by Yoshikatsu Murooka and published by CRC Press. This book was released on 2020-08-26 with total page 894 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bridging the gap between laboratory observations and industrial practices, this work presents detailed information on recombinant micro-organisms and their applications in industry and agriculture. All recombinant microbes, bacteria, yeasts and fungi are covered.

Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by BoD – Books on Demand. This book was released on 2013-05-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.