Download or read book Gold and Silver Staining written by Gerhard W. Hacker and published by CRC Press. This book was released on 2002-04-17 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunogold silver staining is one of the most sensitive techniques available for visualizing the location of antibodies and nucleotide probes that have been bounded to specific antigens or to nucleotide sequences. As gold and silver staining continues to advance research in molecular morphology, this book presents the information you need to know a

Download or read book The Glass Painter s Method written by David Williams and published by . This book was released on 2018-11-12 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will serve you well if: You're new to traditional stained glass painting and are uncertain which brushes, paints and tools to buy - this book will tell you what you want to know. The accompanying 12 free videos will also introduce you to the key techniques. You've painted stained glass for a while but still worry "Is it my lack of skill which is the problem, or is it my brushes, paints and tools which stop me achieving the results I want?" - this book will guide you to the answer. And the 23 free designs will give you an excellent resource with which to practise. You're a teacher who wants your students to experience the joy of mastering this wonderful craft - this book will show you the smallest, most effective set of brushes, paints and tools to place before them so they can triumph. From designers and painters Williams & Byrne, and featuring entries from the journal of the forgotten Victorian craftsman Nathaniel Somers, this book will demonstrate how few possessions you really need to paint stained glass. This is Book 1 of The Glass Painter's Method.

Download or read book Protein Gel Detection and Imaging written by Biji T. Kurien and published by Humana. This book was released on 2019-08-24 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume expands upon the collection of techniques published in Protein Electrophoresis: Methods and Protocols (2012) with more practical and reproducible methods to study protein gel detection and imaging. The chapters in this book cover topics such as coomassie-brilliant blue staining of polyacrylamide gels; silver staining techniques; microwave assisted protein staining, de-staining, and in-solution digestion of proteins; curumin and turmeric as an environment-friendly protein gel stain; in-gel protein phosphotase assay using fluorogenic substrates; destaining with fungal laccase; and radiolabeling and analysis of labeled proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Protein Gel Detection and Imaging: Methods and Protocols is a valuable resource for expert and novice scientists and researchers who are interested in learning and experimenting with this field.

Download or read book Experimental Neurotoxicology Methods written by Jordi Llorens and published by Springer Nature. This book was released on 2021-07-23 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores the latest methods that seek to address the vital questions being asked in neurotoxicology research. The chapters in this book cover a variety of available methods from the molecular level to the organism level, and both in vitro and in vivo approaches, including alternative model organisms. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and authoritative, Experimental Neurotoxicology Methods is a valuable resource for both young and experienced researchers who are looking for guidance to implement these methods in their laboratories or for understanding the data generated through these techniques.

Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.

Download or read book Silver Stain written by J. Kenneth Leap and published by . This book was released on 2012* with total page 79 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Download or read book Protein Electrophoresis written by Biji T. Kurien and published by Humana Press. This book was released on 2012-05-16 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.

Download or read book Clean My Space written by Melissa Maker and published by Penguin. This book was released on 2017-03-07 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: The wildly popular YouTube star behind Clean My Space presents the breakthrough solution to cleaning better with less effort Melissa Maker is beloved by fans all over the world for her completely re-engineered approach to cleaning. As the dynamic new authority on home and living, Melissa knows that to invest any of our precious time in cleaning, we need to see big, long-lasting results. So, she developed her method to help us get the most out of our effort and keep our homes fresh and welcoming every day. In her long-awaited debut book, she shares her revolutionary 3-step solution: • Identify the most important areas (MIAs) in your home that need attention • Select the proper products, tools, and techniques (PTT) for the job • Implement these new cleaning routines so that they stick Clean My Space takes the chore out of cleaning with Melissa’s incredible tips and cleaning hacks (the power of pretreating!) her lightning fast 5-10 minute “express clean” routines for every room when time is tightest, and her techniques for cleaning even the most daunting places and spaces. And a big bonus: Melissa gives guidance on the best non-toxic, eco-conscious cleaning products and offers natural cleaning solution recipes you can make at home using essential oils to soothe and refresh. With Melissa’s simple groundbreaking method you can truly live in a cleaner, more cheerful, and calming home all the time.

Download or read book 2 D Proteome Analysis Protocols written by Andrew J. Link and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

Download or read book Dermatopathology The Basics written by Bruce R. Smoller and published by Springer Science & Business Media. This book was released on 2009-06-10 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dermatopathology: The Basics serves as an effective and efficient handbook for the student of dermatopathology, and as a practical bench reference for the practicing diagnostician who desires rapid access to criteria that are useful in differentiating histologically similar entities. The reader is able to focus upon a single histologic observation, i.e., inflammatory conditions without epidermal changes, and use this as a starting point from which to build a differential diagnosis based upon pattern recognition. As each entity is addressed, there is a concise discussion of the basic clinical findings and epidemiologic associations. This is followed by a histologic description, highlighting areas that serve to discriminate between the entity under discussion and similar ones. Any immunologic studies that might augment the diagnostic sensitivity or specificity are discussed. Chapters are thematically based and consist of essential bullet points arranged in organized outlines allowing for easy access and direct comparison between entities. The salient histologic features are depicted with abundant high quality, full-color photomicrographs placed immediately adjacent to the appropriate histologic bullet points. This volume serves as an effective and efficient handbook for the student of dermatopathology, and as a practical bench reference for the practicing diagnostician who desires rapid access to criteria that are useful in differentiating histologically similar entities. The elaborate pictorial documentation serves as an atlas of the commonest dermatologic disorders.

Download or read book Immunogold Silver Staining written by M. A. Hayat and published by CRC Press. This book was released on 1995-08-03 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.

Download or read book Proteomics written by Stephen R. Pennington and published by Garland Science. This book was released on 2001 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics is an emerging research area, and the book reviews genome projects in relation to the need for complementary proteomics programmes.

Download or read book Two Dimensional Electrophoresis Protocols written by David Sheehan and published by Humana Press. This book was released on 2009-04-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human genome and other large-scale genome sequencing projects have inevitably led to a focus on the proteins encoded by genes. The field of proteomics has grown enormously as a result and a number of high-throughput technologies have now been developed allowing discovery-led investigations of protein populations rather than more traditional hypothesis-led studies on single proteins. These high-throughput techno- gies include gene and protein microarrays, the yeast two-hybrid system, and various mass spectrometry methodologies. However, despite developments and improvements in these technologies, two-dimensional electrophoresis (2DE) remains one of the most widely used approaches. This technique was revolutionised by the development of immobilised pH gradient strips which are now commercially available. This has made possible highly reproducible separations of matched samples. Developments in staining, mass spectr- etry, and bioinformatics supported these developments and have led to a measure of standardisation in design, execution, and analysis of proteomics experiments. This book began life as a proposed update of the excellent volume 2DE Protocols edited by Andrew Link of the University of Washington at Seattle. However, we re- ised that 2DE has undergone major development in aspects of its technology in recent years and we were anxious to reflect these in the present volume. We are also conscious that many researchers have now begun to apply proteomics methodologies to a growing range of biological material and we were anxious to include contributions to reflect the challenges posed in sample preparation in less widely used organisms.

Download or read book Microbiology written by Nina T. Parker and published by . This book was released on 2016-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs.

Download or read book From Research to Manuscript written by Michael Jay Katz and published by Springer Science & Business Media. This book was released on 2006 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt: Demonstrating how to compose a scientific paper, this book describes not just what to do but why and how, explaining why each section of a science paper requires its particular form of information, and showing how to fit data and arguments into that form. It recognizes that experiments in different disciplines need different presentations.

Download or read book Stains and Cytochemical Methods written by M.A. Hayat and published by Springer Science & Business Media. This book was released on 1993-03-31 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.