Download or read book Silver 109 based Laser Desorption ionization Mass Spectrometry Method for Detection and Quantification of Amino Acids written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fundamental Study and Method Development for Surface based Laser Desorption Ionization Imaging Mass Spectrometry written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: By providing both the chemical identity and the spatial organization of each component in biological samples, Imaging Mass Spectrometry (IMS) becomes an emerging tool in clinic and pharmacological study. Most work in IMS has been focused on protein and peptide mapping in biological samples to take advantage of effective analyte ionization in MALDI-MS, and also partially due to the limitation of MALDI-MS in small molecule detection. The focus of my research is to develop novel tools to image spatial distribution of small molecules in biological samples. A surface-based mass spectrometric imaging method, i.e. Desorption/Ionization on Silicon (DIOS), was used for biological surface analysis in the concept-proof investigation. More over, possible proton transferring pathways and impact of local chemical environment have been systematically investigated in the fundamental understanding of ionization mechanism of SALDI-MS. Based on the finding on the SALDI mechanism, a hybrid ionization approach, ME-SALDI has been developed by combing the strength of the conventional MALDI matrix and SALDI, where the improved detection sensitivity with reduced matrix-analyte interference and the improved imaging capability through analysis of mouse brain and heart sections has been demonstrated. In addition, the impact of vacuum stability of matrix in ME-SALDI-IMS applications has been examined. A solvent free, homogenous and reproducible sublimation method has been developed for ionic matrix in ME-SALDI, by which improved vacuum stability and MS detection have been achieved. Furthermore, a new generation of meso-porous oxide substrate was developed as a novel ME-SALDI substrate with a superior storage stability, extended detectable mass range and robust substrate preparation.

Download or read book Laser Desorption Ionization Mass Spectrometry Based on Nanophotonic Structure From Material Design to Mechanistic Understanding written by Moon-Ju Kim and published by Springer Nature. This book was released on 2023-11-11 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents innovative laser desorption ionization (LDI)-active nanophotonic structures for addressing the challenges that matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is currently facing and for enhancing LDI efficiency. It presents a variety of cutting-edge nanophotonic structures to satisfy the mass-analytical needs of sensitivity, reproducibility, and quantification. As opposed to the commercialized, conventional organic matrix used in MALDI-MS, these nanostructures are validated to be highly effective in detecting small metabolites and drugs, highlighting their considerable potential in the mass spectrometry field. It also systematically elucidates fundamental LDI processes in terms of the photo-thermal, electronic, and structural characteristics of nanophotonic structures, offering mechanistic knowledge of LDI-MS. Even though LDI-MS performance is heavily influenced by a number of nanostructure parameters, relatively little is known about the LDI processes associated with those characteristics. An in-depth understanding of nanostructure characteristics and LDI mechanisms thus paves the way for more effective, rational design and development of nanostructures with improved LDI capabilities. Further, with a focus on multiple cascades in nanostructure functions in response to laser pulse stimuli, this book provides detailed, step-by-step procedures to design and construct a nanophotonic, LDI-active platform, which may serve as a roadmap for graduate students in the field of mass spectrometry. Readers, including graduate students, researchers, and experts working in the related areas of mass spectrometry, nanophotonics, and material science and material engineering, will find a wealth of useful information in this book.

Download or read book Quantification by Matrix Assisted Laser Desorption Ionization Mass Spectrometry Using An Approach Based On Stochastic Dynamics Experimental And Theoretical Correspondences written by Bojidarka Ivanova and published by GRIN Verlag. This book was released on 2018-05-14 with total page 89 pages. Available in PDF, EPUB and Kindle. Book excerpt: Research Paper (postgraduate) from the year 2018 in the subject Chemistry - Analytical Chemistry, grade: Mass spectrometry, University of Dortmund (Institut für Umweltforschung, Lehrstuhl für Analytische Chemie), language: English, abstract: The goal of this work is to describe more recent developments in the quantitative mass spectrometry and to illustrate how our new model equations based on the stochastic dynamics relate to the determining the 3D molecular and electronic structures of analytes. It is aimed at researchers in Chemistry who would like to find out about what is going on in mass spectrometric methodological contributions more recently. The work could also be used to MSc and PhD students in the field of Chemistry, in particular, highlighting the ‘Analytical chemistry’, ‘Physico–chemistry’ and/or ‘Computational and theoretical chemistry’, respectively.

Download or read book Nano Antimicrobials written by Nicola Cioffi and published by Springer Science & Business Media. This book was released on 2012-02-26 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a high demand for antimicrobials for the treatment of new and emerging microbial diseases. In particular, microbes developing multidrug resistance have created a pressing need to search for a new generation of antimicrobial agents, which are effective, safe and can be used for the cure of multidrug-resistant microbial infections. Nano-antimicrobials offer effective solutions for these challenges; the details of these new technologies are presented here. The book includes chapters by an international team of experts. Chemical, physical, electrochemical, photochemical and mechanical methods of synthesis are covered. Moreover, biological synthesis using microbes, an option that is both eco-friendly and economically viable, is presented. The antimicrobial potential of different nanoparticles is also covered, bioactivity mechanisms are elaborated on, and several applications are reviewed in separate sections. Lastly, the toxicology of nano-antimicrobials is briefly assessed.

Download or read book Advances in MALDI and Laser Induced Soft Ionization Mass Spectrometry written by Rainer Cramer and published by Springer. This book was released on 2016-08-23 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation and large-scale, high-speed MS sample profiling, new methods based on MALDI or MALDI-like sample preparations, and the advantages of ESI to MALDI MS analysis. This is an ideal book for graduate students and researchers in the field of bioanalytical sciences. This book also: • Showcases new techniques and applications in MALDI MS • Demonstrates how MALDI is preferable to ESI (electrospray ionization) • Illustrates the pros and cons associated with biomarker discovery studies in clinical proteomics and the various application areas, such as cancer proteomics

Download or read book Liquid Chromatography Continuous flow Matrix assisted Laser Desorption Ionization Time of flight Mass Spectrometry for the Detection of Bioactive Peptides and Proteins written by and published by . This book was released on 1997 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the general approach of combining liquid chromatography with matrix-assisted laser desorption ionization mass spectrometry for on-line detection of peptides and proteins, based on the use of a continuous flow probe. Recent advances in time-of-flight mass spectrometric instrumentation for this approach are reported. Finally, the results of time-lag focusing matrix-assisted laser desorption ionization analysis of 15 peptides and proteins using solid matrices are presented. Mass measurement error is discussed along with the limitations of, and potential improvements to, the analytical system proposed.

Download or read book Matrix assisted Laser Desorption ionization Mass Spectrometry written by Pao-Chi Liao and published by . This book was released on 1995 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development and Application of Two step Laser Desorption multiphoton Ionization Mass Spectrometry written by Jong Hoon Hahn and published by . This book was released on 1988 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Matrix assisted Laser Desorption ionization Mass Spectrometric Study of Biologic Molecules written by Yan-Hui Liu and published by . This book was released on 1996 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of Complex Biological Mixtures by Matrix assisted Laser Desorption ionization Mass Spectrometry written by Xiaoli Liang and published by . This book was released on 1997 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book An Investigation of Liquid Secondary Ion and Laser Desorption Mass Spectrometry for the Analysis of Planar Chromatograms written by Jocelyn Clare Dunphy and published by . This book was released on 1990 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of Matrix assisted Laser Desorption ionization and Liquid Chromatography Electrospray Ionization Based Mass Spectrometric Techniques for Characterizing and Quantifying Endogenous and Therapeutic Biomolecules written by Bingming Chen and published by . This book was released on 2017 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: Endogenous biomolecules, such as neuropeptide, protein and carbohydrates, play important roles in biological and physiological processes. Over the last two decades, therapeutic biomolecules, including therapeutic peptides, proteins and monoclonal antibodies, have become large and fast-growing segments of the pharmaceutical industry. However, analyzing biomolecules is challenging due to their large size, complex structure and various modifications. In situ analysis of biomolecules is even more challenging due to the large dynamic range and complex microenvironments on tissue surface. In this dissertation, matrix-assisted laser desorption/ionization (MALDI) and liquid chromatography (LC) -- electrospray ionization (ESI) based mass spectrometric techniques were developed to characterize and quantify endogenous and therapeutic biomolecules. Specifically, this work developed novel ionization techniques and mass spectrometry imaging workflows to study the sequence and in situ distribution of biomolecules on high resolution accurate mass instrument platforms. Moreover, absolute or relative quantifications of biomolecules, such as therapeutic peptide or N-glycans, were measured by cutting-edge quantification workflows. In summary, this work not only improves current analytical methodology for characterizing biomolecules, but also demonstrates its potential applications in pharmaceutical industry and clinical settings.

Download or read book The Use of Active Substrates and Membranes in Matrix assisted Laser Desorption ionization Mass Spectroscopy written by Jian Bai and published by . This book was released on 1996 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fundamental Study and Method Development for Surface based Laser Desorption Ionization Imaging Mass Spectrometry written by Qiang Liu and published by . This book was released on 2009 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: machaniam, metabolite, mass spectrometry, imaging mass spectrometry, MS.

Download or read book Fundamental Studies of Matrix assisted Laser Desorption ionization Time of flight Mass Spectrometry MALDI TOF MS and Its Applications to the Analysis of Biological Materials written by Yongdong Zhu and published by . This book was released on 1999 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Smart Materials in Analytical Chemistry written by Miguel de la Guardia and published by John Wiley & Sons. This book was released on 2019-01-22 with total page 931 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to smart materials and how they are used in sample preparation, analytical processes, and applications This comprehensive, two-volume handbook provides detailed information on the present state of new materials tailored for selective sample preparation and the legal frame and environmental side effects of the use of smart materials for sample preparation in analytical chemistry, as well as their use in the analytical processes and applications. It covers both methodological and applied analytical aspects, relating to the development and application of new materials for solid-phase extraction (SPE) and solid-phase microextraction (SPME), their use in the different steps and techniques of the analytical process, and their application in specific fields such as water, food, air, pharmaceuticals, clinical sciences and forensics. Every chapter in Handbook of Smart Materials in Analytical Chemistry is written by experts in the field to provide a comprehensive picture of the present state of this key area of analytical sciences and to summarize current applications and research literature in a critical way. Volume 1 covers New Materials for Sample Preparation and Analysis. Volume 2 handles Analytical Processes and Applications. Focuses on the development and applications of smart materials in analytical chemistry Covers both, methodological and applied analytical aspects, for the development of new materials and their use in the different steps and techniques of the analytical process and their application in specific fields Features applications in key areas including water, air, environment, pharma, food, forensic, and clinical Presents the available tools for the use of new materials suitable to aid recognition process to the sample preparation and analysis A key resource for analytical chemists, applied laboratories, and instrument companies Handbook of Smart Materials in Analytical Chemistry, 2V Set is an excellent reference book for specialists and advanced students in the areas of analytical chemistry, including both research and application environments.