Download or read book Silica Nanoparticle Platform to Generate New Molecular Probes for Fluorescence Lifetime Imaging written by Soo Young Song and published by . This book was released on 2016 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence microscopy is the most widely used detection method to image molecular probes, but is currently hindered due to the limited number of fluorescent probes available. The fluorescence lifetime of fluorescent probes that is the time an excited fluorophore takes to return to the ground state; this thesis studied a novel system to manipulate the fluorescent molecules' lifetime using the nanoprobe format. This system can exponentially increase the number of fluorescent probes. The first part of this thesis reports ̃130nm silica nanoparticles labeled with silanized fluorescent dyes -- Oregon Green 488, BODIPY, and rhodamine -- using various total dye loadings. As the total dye loading increased, the relative fluorescence efficiency decreased due to self-quenching. This self-quenching effect was characterized by the phasor approach to fluorescence lifetime that gave unique and distinguishable spatial values of the nanoparticles. Utilizing the synthetic versatile properties of silica in accommodating other materials, the second part of this thesis discussed core-shell structured silica nanoparticles with an independent control to the fluorescence lifetimes of the cores and the shells. 35nm Silica cores were synthesized using a reverse microemulsion method. Various concentrations of fluorescent nanomaterials encapsulated inside the cores govern the cores' phasor values. Subsequently, silica shells with various thicknesses were synthesized using the Stober sol-gel reaction. Various total dye loadings govern the shells' phasor values while the shell thicknesses control their fluorescence intensity. The distinct fluorescence lifetime phasor values enable nanoprobes to have visually effective representations that can be easily expanded by simply manipulating the molecular proximities between fluorophores.
Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.
Download or read book Synthesis Characterization and Optical Properties of Colloidal Organic Dye containing Fluorescent Core shell Silica Nanoparticles written by Erik Herz and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since fluorescence was first observed in a solution containing quinine sulfate in 1845, methods employing fluorescent materials have abounded. Today, fluorescent probes are widely used in many fields, including biological imaging and security. For all applications brightness, stability, size control and compatibility are key parameters for probe design. Recent research to address these parameters has been focused on synthesizing nanoparticle materials. A class of those materials, core-shell silica nanoparticles, covalently encapsulating organic dyes within the core, have been found to be particularly effective in meeting the need for brighter, more stable probes, while also providing size and compatibility control. This dissertation will describe how I have built on previous research by focusing on the synthesis, characterization and optical properties of core-shell silica nanoparticles that span the full spectral range, from ultraviolet to near-infrared emission. Through scattering corrected comparisons of coumarin dye-containing nanoparticles and dye in aqueous solution, relative quantum yield enhancements of an order of magnitude were measured. Similar brightness enhancements were observed when large Stokes-shift dyes were incorporated into the particles. By creating a graded series of Stokes-shifted particles, single-excitation functionality was added to the fluorescent core-shell silica nanoparticle platform, to allow for spectral multiplexing. Since both light scattering and autofluorescence backgrounds in biological samples tend to be stronger at shorter wavelengths, new probe design has swiftly moved toward near-infrared (NIR) excitation and emission. To this end, NIR fluorescent silica nanoparticles with emissions up to 800nm were synthesized and dye structure-optical property correlations were investigated. Using a self-built fluorescence correlation spectroscopy (FCS) instrument, cyanine dye containing particles of the NIR series were investigated in further depth. The FCS allows for measurement of the size, brightness and concentration of the particles in solution and, with small modifications to the beam-path also provides information on the fluorescence stability of the particles in solution, which is of particular importance in NIR probes. Photostability in aqueous solution was investigated for the NIR dye Cy5 and compared to the dye incorporated into particles, leading to findings that will impact future probe designs.
Download or read book Silica Based Nanotechnology for Bone Disease Treatment written by Mariela Agotegaray and published by Springer Nature. This book was released on 2021-01-04 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses conventional treatments of bone diseases. It proposes nanotechnological tools based on the use of silica as a bioactive material for bone. The book links the physiological and anatomical aspects of the bone with the ancestral importance of the bone system. It describes associated pathologies and conventional treatments, emphasizing their shortcomings. The book gives future perspectives and applicable tools to the development of new nanomaterials.
Download or read book Growth of Silica based Nanoparticles written by Teresa Yi Kao and published by . This book was released on 2017 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: Silica chemistry provides a uniquely tunable platform for nanoparticle synthesis, where particle size, nanoscale morphology, and surface properties can be precisely controlled. Recent advances demonstrate that conveniently accessible parameters, including silica precursor chemistry, solvent, and reaction pH, can be used to tune particle size down to below 10 nm. By cooperative assembly of inorganic silica species and organic molecular structure directing agents, a diverse range of mesoporous silica nanoparticles with hexagonal, cubic, and multicompartment structures can be produced. This versatile chemistry provides pathways for answering fundamental questions about structure formation and developing novel functional nanomaterials for applications including separation, catalysis, and drug delivery. In this dissertation, two examples of such silica nanoparticle systems are discussed. As a first example, the development of an intensity-based fluorescent silica nanoparticle barcode is discussed. This work is motivated by a need for fluorescent tags that increase the number of molecular species that can be simultaneously labeled and reliably distinguished using commercially available fluorescence microscopes. In this study, the synthetic parameters that govern the incorporation of precisely controlled numbers of fluorescent dyes into silica nanoparticles in batch reactions are identified. Heterogeneities within particle batches are mapped using single particle fluorescence microscopy. Proof-of-concept experiments demonstrate that fluorescent silica nanoparticles with well-separated high and low fluorescence intensity distribution levels can be synthesized in batch reactions and used as an intensity barcode in fluorescence microscopy. In the second example, a mesoporous silica nanoparticle system, structure directed by surfactant-micelle self-assembly, is investigated. As a function of an added pore expander molecule or reaction stirring rate, a series of four distinct mesoporous silica nanoparticle structures is observed: hexagonal, cubic/hexagonal multicompartment, cubic, and dodecagonal quasicrystalline. The mechanism driving the structural transition between cubic crystalline and dodecagonal quasicrystalline mesoporous silica nanoparticles is investigated. Control of nanoparticle size down to a single tiling unit (
Download or read book Fluorescent Gold silver Alloy Core Silica Shell Nanoparticles for Bioimaging Applications written by Siyu Tu and published by . This book was released on 2016 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials have been a popular research field for decades and scientists are always trying to develop novel materials. This can be achieved with a hybrid of existing material to either significantly improve the performance for certain applications or generate new properties which can be exploited for new applications. Two perfect examples are gold-silver (Au/Ag) alloy nanoparticles (NPs) with strong and tunable extinction spectra in the visible range and superluminescent NPs with a metallic core and dye-doped silica shell. Both of them can be used as contrast agents for biomedical imaging applications. This research is based on the combination of these two types of NPs: the demonstration of a multiplexed bioimaging application with fluorescent NPs composed of a Au/Ag alloy core and a fluorophore-doped silica shell(Au/Ag SiO2). It has long been known that immunofluorescence, as the standard technique to label cells, has some drawbacks such as intensity limit, limited multiplexing and loss of signal due to photobleaching. Plasmonic NPs do not photobleach and do not lose their scattering signal. The Au/Ag alloy NPs with a core-shell structure not only exploit the merits of a tunable scattering signal from the core but also the metal enhanced fluorescence (MEF) effect from the shell near a metallic surface. In this research, we have fabricated the hybrid NPs and applied them in a multimodal platform to detect both scattering and fluorescence signals in order to improve the multiplexing capability. During the master study, Au/Ag SiO2 NPs were successfully fabricated with high reproducibility. Different fluorophores with different emission wavelengths were incorporated in the silica shell of Au/Ag SiO2 NPs resulting in a stronger fluorescence signal in the presence of a metallic core. An etching effect of the metallic core was noted during the silica shell growth, creating a spacer between the surface of the metallic core and silica shell resulting in a slight blueshift of the plasmonic peak. This effect is more severe for silver-rich cores. Four types of NPs were prepared, with two different Au/Ag composition ratios (pure Ag and 50/50 Au/Ag) and two different fluorophores (fluorescein and rhodamine B) were incorporated into the silica shell for a bimodal multiplexed imaging test in scattering and fluorescence. A proportion of 86% of NPs on a glass slide can be identified and a similar result was obtained for NPs on cells.
Download or read book Development and Applications of Fluorescent Core shell Silica Nanoparticles for Bioimaging and Sensing written by Andrew Arthur Burns and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nano-sized fluorescent materials have demonstrated great potential in the emergent fields of nanobiotechnology and nanomedicine. To create effective probes for these applications, high brightness, photostability and size control are key parameters for particle design. Core-shell silica nanoparticles incorporating covalently bound dyes in the particle core have been shown to exhibit significant enhancements in dye brightness and photostability while allowing independent control of particle size, color and surface chemistry. Building on this platform, the first part of this dissertation explores a family of quantitative chemical sensors based on the incorporation of analyte-sensitive dyes into the particle shell. By co-localizing sensor and reference dye molecules onto a single particle, local analyte concentration changes can be discerned from local sensor concentration variations by comparison of the sensor and reference intensities facilitating quantitative "ratiometric" chemical imaging. The core-shell architecture is ideal for this application because it sequesters the reference dye in the particle core, while allowing analyte interactions with the sensor dyes near the particle surface. Traditional Stöber-derived sol-gel routes as well as reverse micelle-based methods were employed to generate pH- and Ca+2-sensitive nanoparticles. Specific sensor architectures were applied to intracellular as well as large-scale functional volumetric imaging of intact biofilms and their metabolic activities. To fully realize the potential of fluorescent nanomaterials for biomedical applications, one of the key obstacles is clearance from the body. The second part of this dissertation investigates several of the key parameters for effective clearance (size and surface chemistry) and highlights the development of a series of particles capable of rapid and efficient bodily clearance and demonstrates their efficacy in mice as a model system.
Download or read book Development of New Fluorescent Silica and Multifunctional Nanoparticles for Bio imaging and Diagnostics written by Arnaud Lemelle and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Silica nanoparticles are effective fluorophore carriers with high potential in imaging, diagnostics, and therapy. The particles are resistant to drastic change of environmental conditions (pH, temperature etc.) and insulate the dyes so as to protect them from photobleaching. Silica chemistry is also versatile and affords an easy modification of the particle composition and surface to integrate targeting ligands or to integrate other nanoparticles. Regardless of their advantages, there exists a lack of dye diversity in the literature that is connected to a low affinity for potential tools for biology and medicine. This thesis describes the developmentof an alternative method for the synthesis of fluorescent silica nanoparticles and their modification to incorporate iron oxide and gold.
Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Download or read book Fluorine Magnetic Resonance Imaging written by Ulrich Flogel and published by CRC Press. This book was released on 2016-10-26 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past decade, fluorine (19F) magnetic resonance imaging (MRI) has garnered significant scientific interest in the biomedical research community owing to the unique properties of fluorinated materials and the 19F nucleus. Fluorine has an intrinsically sensitive nucleus for MRI. There is negligible endogenous 19F in the body and thus there is no background signal. Fluorine-containing compounds are ideal tracer labels for a wide variety of MRI applications. Moreover, the chemical shift and nuclear relaxation rate can be made responsive to physiology via creative molecular design. This book is an interdisciplinary compendium that details cutting-edge science and medical research in the emerging field of 19F MRI. Edited by Ulrich Flögel and Eric Ahrens, two prominent MRI researchers, this book will appeal to investigators involved in MRI, biomedicine, immunology, pharmacology, probe chemistry, and imaging physics.
Download or read book Molecular Beacons written by Chaoyong James Yang and published by Springer Science & Business Media. This book was released on 2013-08-15 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.
Download or read book Lanthanide Luminescence written by Pekka Hänninen and published by Springer Science & Business Media. This book was released on 2011-06-21 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lanthanides have fascinated scientists for more than two centuries now, and since efficient separation techniques were established roughly 50 years ago, they have increasingly found their way into industrial exploitation and our everyday lives. Numerous applications are based on their unique luminescent properties, which are highlighted in this volume. It presents established knowledge about the photophysical basics, relevant lanthanide probes or materials, and describes instrumentation-related aspects including chemical and physical sensors. The uses of lanthanides in bioanalysis and medicine are outlined, such as assays for in vitro diagnostics and research. All chapters were compiled by renowned scientists with a broad audience in mind, providing both beginners in the field and advanced researchers with comprehensive information on on the given subject.
Download or read book Radiative Decay Engineering written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2007-12-11 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: During recent years our enthusiasm for this field has continually increased. This book presents expert contributions describing the fundamental principles for the widespread use of radiative decay engineering in the biological sciences and nanotechnology.
Download or read book Semiconductor Nanomaterials written by Challa S. S. R. Kumar and published by John Wiley & Sons. This book was released on 2010-04-05 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book series Nanomaterials for the Life Sciences, provides an in-depth overview of all nanomaterial types and their uses in the life sciences. Each volume is dedicated to a specific material class and covers fundamentals, synthesis and characterization strategies, structure-property relationships and biomedical applications. The series brings nanomaterials to the Life Scientists and life science to the Materials Scientists so that synergies are seen and developed to the fullest. Written by international experts of various facets of this exciting field of research, the series is aimed at scientists of the following disciplines: biology, chemistry, materials science, physics, bioengineering, and medicine, together with cell biology, biomedical engineering, pharmaceutical chemistry, and toxicology, both in academia and fundamental research as well as in pharmaceutical companies. VOLUME 6 - Semiconductor Nanomaterials
Download or read book Advanced Photon Counting written by Peter Kapusta and published by Springer. This book was released on 2015-04-23 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Download or read book Fluorescent Materials for Cell Imaging written by Fu-Gen Wu and published by Springer Nature. This book was released on 2020-10-26 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.
Download or read book Modern Tools for Time Resolved Luminescence Biosensing and Imaging written by and published by Frontiers Media SA. This book was released on 2021-09-01 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:
