Download or read book Self Assembling Complexes for Gene Delivery written by Alexander V. Kabanov and published by Wiley-Blackwell. This book was released on 1998-05-29 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent years have witnessed an explosion of activity in the field of gene therapy. Following advances in our understanding of the molecular basis of disease, hopes are high that the tremendous potential market for drugs employing antisense nucleotides and genes will one day be fulfilled. However, many obstacles remain, not least problems in the technology of gene delivery systems. Much of today's research focuses on non-viral approaches to gene delivery. Of particular importance are supramolecular complexes formed between DNA and various natural and synthetic polymers and lipids, otherwise known as 'self-assembling complexes for gene delivery'. In view of this fact, the editors of this volume have assembled an international team of contributors to present up-to-date reviews of the major chemical, biological and clinical aspects of such gene delivery vectors. Reflecting the diversity of research in this field, this book comprehensively covers: * the principles of self-assembly * natural mechanisms for gene delivery to cells * cationic lipids and liposomes * polyelectrolyte DNA complexes * systemic biodistribution of drug delivery systems * targeting of conjugates for gene delivery * new approaches to gene delivery * clinical evaluation Self-assembling Complexes for Gene Delivery is an essential reference for all professionals with an interest in gene and antisense therapy or novel drug delivery systems, including medicinal and pharmaceutical chemists, clinicians, human geneticists, molecular biologists and pharmacologists.

Download or read book Artificial Self assembling Systems for Gene Delivery written by Philip L. Felgner and published by . This book was released on 1996 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses recent advances in artificial self-assembling systems, including retrotransposon vectors, hairpin ribozymes, triple-helix-forming oligonucleotides, liposome-mediated transfection, and photonic nanostructures. Addresses developing efficient synthetic vectors such as modular self-assembling systems mimicking important features of viral vectors. Presents new developments in synthetic self-assembling gene delivery systems, including innovations in nonviral systems, targeting nucleic acids, ligand-polylysine mediated transfer, dendrimer-mediated transfection, cationic liposomes, and polylysine DNA complexes.

Download or read book Synthetic DNA Delivery Systems written by Dan Luo and published by Springer Science & Business Media. This book was released on 2003-09-30 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function. Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest. Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Download or read book Developing Gene Delivery Vehicles Using Self assembly written by Chung-Huei Katherine Wang and published by . This book was released on 2011 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Designer Biopolymers written by Ayae Sugawara-Narutaki and published by MDPI. This book was released on 2020-12-14 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nature has evolved sequence-controlled polymers, such as DNA and proteins, over its long history. The recent progress of synthetic chemistry, DNA recombinant technology, and computational science, as well as the elucidation of molecular mechanisms in biological processes, drive us to design ingenious polymers that are inspired by naturally occurring polymers, but surpass them in specialized functions. The term “designer biopolymers” refers to polymers which consist of biological building units, such as nucleotides, amino acids, and monosaccharides, in a sequence-controlled manner. This book particularly focuses on the self-assembling aspect of designer biopolymers. Self-assembly is one common feature in biopolymers that is used to realize their dynamic biological activities and is strictly controlled by the sequence of biopolymers. In a broad sense, the self-assembly of biopolymers includes a double-helix formation of DNA, protein folding, and higher-order protein assembly (e.g., viral capsids). Designer biopolymers are now going beyond what nature evolved: researchers have generated DNA origami, protein cages, peptide nanofibers, and gels. This book illustrates the latest interdisciplinary work on self-assembling designer biopolymers. As shown by this book, the self-assembly of biopolymers has a great impact on a variety of research fields, including molecular biology, neurodegenerative diseases, drug delivery, gene therapy, regenerative medicine, and biomineralization. Designer biopolymers will help researchers to better understand biological processes, as well as to create innovative molecular systems. We believe that this book will provide readers with new ideas for their molecular design strategies for frontier research.

Download or read book Non viral Gene Delivery Vectors Based on New Unsymmetrical Bolaamphiphiles written by Namrata Jain and published by . This book was released on 2010 with total page 209 pages. Available in PDF, EPUB and Kindle. Book excerpt: The success in gene therapy relies strongly on new efficient gene delivery vectors. Non-viral vectors are highly attractive, since they can deliver large quantities of genetic information and are low immunogenic. In this respect, unsymmetrical bolaamphiphiles (bolas) bearing two different hydrophilic head-groups connected by a hydrophobic spacer could be an attractive alternative for vector design, as they can form well-defined supramolecular nanostructures (nanovesicles and nanotubes) that are more stable than those formed with lipids. The aim of the present work was to develop new gene delivery vectors based on bolas capable to form such nanostructures hosting a DNA molecule and exposing at their external surface neutral ligands. For this purpose, a variety of bolas, bearing cationic and neutral groups connected by a hydrophobic spacer, were obtained by multi-step organic synthesis. Their characterization by different instrumental techniques suggested that the nature of the head groups as well as the hydrophobic spacer defines the bola self-assembly, their interaction with DNA and the morphology of their complexes (bolaplexes). While the first two generations of bolas lack the essential features of nonviral vectors, the final third generation was found highly promising. The latter showed a strong interaction with DNA, the formation of small bolaplexes and good transfection efficiency in different cell lines. The increase in transfection efficiency in presence of DOPE or chloroquine suggested that the key barrier for their internalization could be the lipid fusion and the endosomal escape. Some bolaplex formulations showed a transfection efficiency comparable to the best commercial transfection agents. Thus, the present work introduces a new class of efficient transfection agents based on unsymmetrical bolaamphiphiles.

Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

Download or read book Molecular Mechanisms in Lipid based Gene Delivery Systems written by Ayesha Ahmad and published by . This book was released on 2004 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Structure function Properties of Self assembled Lipid short Nucleic Acid Complexes written by Nathan Francis Bouxsein and published by . This book was released on 2010 with total page 614 pages. Available in PDF, EPUB and Kindle. Book excerpt: We report on the gene silencing activities of Cationic Liposome (CL)-siRNA complexes transfected into cultured mammalian cells. We show that traditional CL formulations are inefficient delivery vectors for siRNA, requiring much larger CL:NA charge ratios for measurable activity. The high amount of CL required to achieve these charge ratios induces cellular toxicity, however, complex formulations with multivalent (5+) CLs can alleviate this effect. Additionally, we present new structural data suggesting that the internal organization of the siRNA within the complex is isotropic and not optimally packed. Poor packing negatively influences the stability of the complex and is related to the lower delivery efficiencies. Synchrotron x-ray diffraction experiments on model CL-short DNA complexes revealed that end stacking of the duplexes will promote a novel, highly ordered centered rectangular phase of DNA within the complex - in contrast to the unorganized siRNA. Furthermore, in systems designed to inhibit end stacking, the organization became dependent on the shape anisotropy of the NA, favoring longer sequences over shorter sequences. Taken together, we suggest some criteria for CL-short NA formulations that improve their overall stability and build towards more efficient CL-siRNA complexes for gene silencing.

Download or read book Self assembly of Cationic Lipoplexes from Liposomes and Plasmids of Variable Size written by Elisabete Gonçalves and published by . This book was released on 2003 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Synthesis and Characterization of Environmentally Responsive Lipid nucleic Acid Complexes for Applications in Gene Delivery and Biomaterials written by Rahau S. Shirazi and published by . This book was released on 2012 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmentally responsive materials, i.e., materials that are designed to react to changes in their environment with a modification in their properties or structure, draw growing interests especially for synthetic materials for gene therapy and biomaterials applications. In this work, environmentally responsive lipid materials are designed to be responsive to (i) reducing milieu of the cytoplasm and (ii) low pH of late endosome. (i) The cleavable multivalent lipids (CMVLs) are designed with a disulfide bond in the linker between cationic headgroup and hydrophobic tails. CMVLs are designed to show the effect of lipid-nucleic acid complexes degradation in cytoplasm on nucleic acid delivery and cytotoxicity (ii) An acid-labile PEG-lipid (HPEG2K-lipid) capable of losing PEG chains at the pH of late endosomes is tailored for optimum circulation lifetime of liposomes as well as efficient endosomal release. Here, the efficient synthesis is presented as well as extensive characterization of the series of tailored cleavable multivalent lipids (CMVLn, with n = 2 to 5 the number of positive headgroup charges at full protonation). The self-assembled complexes of the CMVLs and DNA are a prototypical environmentally responsive material, undergoing extensive structural rearrangement when exposed to reducing agents. CMVL-DNA complexes are employed as carriers for nucleic acids in DNA transfection as well as small interfering RNA silencing experiments. The employment of CMVLs in complexes with nucleic acids presents the effect of CMVLs degradation on cytotoxicity, DNA transfection as well as siRNA silencing efficiency. The investigation of structural evolution of CMVL-DNA complexes at varied complex composition, temperature and incubation time was assessed employing small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS).

Download or read book Molecular Beacons written by Chaoyong James Yang and published by Springer Science & Business Media. This book was released on 2013-08-15 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Beacons explains working principle of molecular beacons, discusses their design, synthesis, purification and characterization, explores their thermodynamic and kinetic properties, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications. This book is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice. Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.

Download or read book Materials Nanoarchitectonics written by Katsuhiko Ariga and published by Elsevier. This book was released on 2023-12-15 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Materials Nanoarchitectonics: From Integrated Molecular Systems to Advanced Devices provides the latest information on the design and molecular manipulation of self-organized hierarchically structured systems using tailor-made nanoscale materials as structural and functional units. The book is organized into three main sections that focus on molecular design of building blocks and hybrid materials, formation of nanostructures, and applications and devices. Bringing together emerging materials, synthetic aspects, nanostructure strategies, and applications, the book aims to support further progress, by offering different perspectives and a strong interdisciplinary approach to this rapidly growing area of innovation. This is an extremely valuable resource for researchers, advanced students, and scientists in industry, with an interest in nanoarchitectonics, nanostructures, and nanomaterials, or across the areas of nanotechnology, chemistry, surface science, polymer science, electrical engineering, physics, chemical engineering, and materials science. Offers a nanoarchitectonic perspective on emerging fields, such as metal-organic frameworks, porous polymer materials, or biomimetic nanostructures Discusses different approaches to utilizing "soft chemistry" as a source for hierarchically organized materials Offers an interdisciplinary approach to the design and construction of integrated chemical nano systems Discusses novel approaches towards the creation of complex multiscale architectures

Download or read book Self assembling Biomaterials written by Helena S. Azevedo and published by Woodhead Publishing. This book was released on 2018-04-17 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Self-assembling biomaterials: molecular design, characterization and application in biology and medicine provides a comprehensive coverage on an emerging area of biomaterials science, spanning from conceptual designs to advanced characterization tools and applications of self-assembling biomaterials, and compiling the recent developments in the field. Molecular self-assembly, the autonomous organization of molecules, is ubiquitous in living organisms and intrinsic to biological structures and function. Not surprisingly, the exciting field of engineering artificial self-assembling biomaterials often finds inspiration in Biology. More important, materials that self-assemble speak the language of life and can be designed to seamlessly integrate with the biological environment, offering unique engineering opportunities in bionanotechnology. The book is divided in five parts, comprising design of molecular building blocks for self-assembly; exclusive features of self-assembling biomaterials; specific methods and techniques to predict, investigate and characterize self-assembly and formed assemblies; different approaches for controlling self-assembly across multiple length scales and the nano/micro/macroscopic properties of biomaterials; diverse range of applications in biomedicine, including drug delivery, theranostics, cell culture and tissue regeneration. Written by researchers working in self-assembling biomaterials, it addresses a specific need within the Biomaterials scientific community. Explores both theoretical and practical aspects of self-assembly in biomaterials Includes a dedicated section on characterization techniques, specific for self-assembling biomaterials Examines the use of dynamic self-assembling biomaterials

Download or read book Self assembly of Drug polymer Complexes written by Mireia Oliva and published by . This book was released on 2003 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Electrospun Materials and Their Allied Applications written by Inamuddin and published by John Wiley & Sons. This book was released on 2020-05-27 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to explore the history, fundamentals, manufacturing processes, optimization parameters, and applications of electrospun materials. The book includes various types of electrospun materials such as antimicrobial, smart, bioinspired systems. It focuses on the many application areas for electrospun materials such as energy storage and harvesting, catalysis, biomedical including gene delivery and tissue engineering, separation, adsorption and water treatment technologies, packaging. The book emphasizes the enhanced sustainable properties of electrospun materials, with the challenges and future developments being discussed in detail. The chapters are written by top-class researchers and experts from throughout the world.

Download or read book Nonviral Vectors for Gene Therapy written by Mark A. Findeis and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this volume of Methods in Molecular Medicine is to set forth examples of the great variety of techniques and applications that are now emerging in the field of nonviral gene therapy. The book emphasizes not only specific approaches to gene delivery but, in particular, the best current me- ods to prepare, handle, and characterize gene delivery agents. These topics are of very broad importance since gene therapy evolves from its mostly ac- emy-based experimental and clinical research to the ever increasing number of industry-driven programs directed toward commercial development. S- cessful introduction of nonviral gene therapy agents into the clinic should be expected to require rigorous manufacturing and analytical methods that readily meet the regulatory guidelines under which new drug candidates are reviewed for marketing approval. Exactly what those guidelines will prove to be c- tainly depends on the established guidelines for review of both biological and chemical therapeutics. Additionally, many new techniques are being devised and applied to gene therapy research; these techniques will be instrumental in developing and characterizing successful gene delivery agents. Nonviral Vectors for Gene Therapy: Methods and Protocols has two main sections. To start with, there is a series of chapters on specific protocols for the synthesis, characterization, and application of gene delivery agents. S- eral chapters address the topic of materials to bind with DNA to form the compact condensed phases that facilitate cellular delivery.