Download or read book Recombinant DNA Methodology written by and published by Academic Press. This book was released on 2014-05-19 with total page 793 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. - Enzymes in DNA research - DNA isolation, hybridization, and cloning - DNA sequence analysis - cDNA cloning - Gene products - Identification of cloned genes and mapping of genes - Monitoring cloned gene expression - Cloning and transferring of genes into yeast cells - Cloning and transferring of genes into plant cells - Cloning and transferring of genes into animal cells - Site-directed mutagenesis - Protein engineering - Expression vectors

Download or read book Recombinant DNA Technology written by Keya Chaudhuri and published by The Energy and Resources Institute (TERI). This book was released on 2013-01-01 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences.

Download or read book Enzymology Primer for Recombinant DNA Technology written by Hyone-Myong Eun and published by Elsevier. This book was released on 1996-06-03 with total page 727 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. - Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology - Presents information in an easily accessible format to serve as a quick reference source - Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques

Download or read book Recombinant DNA Technology written by Siddra Ijaz and published by Cambridge Scholars Publishing. This book was released on 2019-08-13 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Oversight and Review of Clinical Gene Transfer Protocols written by Institute of Medicine and published by National Academies Press. This book was released on 2014-03-27 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene transfer research is a rapidly advancing field that involves the introduction of a genetic sequence into a human subject for research or diagnostic purposes. Clinical gene transfer trials are subject to regulation by the U.S. Food and Drug Administration (FDA) at the federal level and to oversight by institutional review boards (IRBs) and institutional biosafety committees (IBCs) at the local level before human subjects can be enrolled. In addition, at present all researchers and institutions funded by the National Institutes of Health (NIH) are required by NIH guidelines to submit human gene transfer protocols for advisory review by the NIH Recombinant DNA Advisory Committee (RAC). Some protocols are then selected for individual review and public discussion. Oversight and Review of Clinical Gene Transfer Protocols provides an assessment of the state of existing gene transfer science and the current regulatory and policy context under which research is investigated. This report assesses whether the current oversight of individual gene transfer protocols by the RAC continues to be necessary and offers recommendations concerning the criteria the NIH should employ to determine whether individual protocols should receive public review. The focus of this report is on the standards the RAC and NIH should use in exercising its oversight function. Oversight and Review of Clinical Gene Transfer Protocols will assist not only the RAC, but also research institutions and the general public with respect to utilizing and improving existing oversight processes.

Download or read book Recombinant DNA Technology written by Aleš Prokop and published by Allied Publishers. This book was released on with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Recombinant DNA Technology written by Sardul Singh Sandhu and published by I. K. International Pvt Ltd. This book was released on 2013-12-30 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduces the basic principles and techniques of recombinant DNA. The book begins with an introduction to the different tools used for gene cloning. The final chapters cover the application of Recombinant Technology to current research and provide an inside look at the human genome project, ribozyme technology, antisense technology, DNA sequencing, and protein engineering.

Download or read book Recombinant DNA written by James D. Watson and published by Macmillan. This book was released on 1992-02-15 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of recombitant DNA techniques and surveys advances in recombinant molecular genetics, experimental methods and their results.

Download or read book Recombinant DNA Genes and Genomes written by James D. Watson and published by Macmillan. This book was released on 2007 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA, Third Edition, is an essential text for undergraduate, graduate, and professional courses in Genomics, Cell and Molecular Biology, Recombinant DNA, Genetic Engineering, Human Genetics, Biotechnology, and Bioinformatics. The Third Edition of this landmark text offers an authoritative, accessible, and engaging introduction to modern, genome-centered biology from its foremost practitioners. The new edition explores core concepts in molecular biology in a contemporary inquiry-based context, building its coverage around the most relevant and exciting examples of current research and landmark experiments that redefined our understanding of DNA. As a result, students learn how working scientists make real high-impact discoveries. The first chapters provide an introduction to the fundamental concepts of genetics and genomics, an inside look at the Human Genome Project, bioinformatic and experimental techniques for large-scale genomic studies, and a survey of epigenetics and RNA interference. The final chapters cover the quest to identify disease-causing genes, the genetic basis of cancer, and DNA fingerprinting and forensics. In these chapters the authors provide examples of practical applications in human medicine, and discuss the future of human genetics and genomics projects.

Download or read book Industrial Applications of Recombinant DNA Techniques written by United States. Congress. Senate. Committee on Commerce, Science, and Transportation. Subcommittee on Science, Technology, and Space and published by . This book was released on 1980 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biotechnology 4 written by S. Mahesh and published by New Age International. This book was released on 2003 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Book Is Designed As Per The Syllabus Of Biotechnology Paper Iv Prescribed By Bangalore University. It Also Fully Covers The Second Year Degree Biotechnology Vocational Course Prescribed By The University Grants Commission (Ugc), New Delhi. The Book Is Divided Into Three Parts As Follows: * Recombinant Dna Technology * Environmental Biotechnology * Animal Cell CultureThe Presentation In Each Part Is Simple And Systematic.The Basic Concepts Have Been Clearly Explained And Their Functions Are Adequately Highlighted. A Few Recent Developments Have Also Been Included To Provide A Contemporary Understanding Of The Subject.

Download or read book Basic Concept of Recombinant DNA Technology written by Somnath De and published by EduPedia Publications (P) Ltd. This book was released on 2016-02-22 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: I am very glad to present this book of Basic Concept of Recombinant DNA Technology, written according to revised syllabus of B.Sc, M.Sc(Biotechnology, Microbiology), B.Pharm, M.Pharm, M.Sc Agriculture and Veterinary in all Indian Universities. This book is also useful for the medical students. I extend my good wishes to the students and teachers of Biotechnology and Microbiology, sincerely hope that Basic Concept of Recombinant DNA Technology, will receive a warm welcome from them. I welcome comments by readers of Basic Concept of Recombinant DNA Technology, for way to improve the book and to increase its value. Such suggestions will be seriously considered in the preparation of subsequent editions. I am very grateful to Dr. Tanusri Mandal, Associate Professor and Head, Department of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, India for useful suggestions and help made by her time to time. Finally, I would like to thanks my wife Arpita Pattanayak(De), and my sweet daughter Anindita De for continuous encouragement for completion of this book.

Download or read book Introduction to Basics of Pharmacology and Toxicology written by Gerard Marshall Raj and published by Springer Nature. This book was released on 2019-11-16 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book illustrates, in a comprehensive manner, the most crucial principles involved in pharmacology and allied sciences. The title begins by discussing the historical aspects of drug discovery, with up to date knowledge on Nobel Laureates in pharmacology and their significant discoveries. It then examines the general pharmacological principles - pharmacokinetics and pharmacodynamics, with in-depth information on drug transporters and interactions. In the remaining chapters, the book covers a definitive collection of topics containing essential information on the basic principles of pharmacology and how they are employed for the treatment of diseases. Readers will learn about special topics in pharmacology that are hard to find elsewhere, including issues related to environmental toxicology and the latest information on drug poisoning and treatment, analytical toxicology, toxicovigilance, and the use of molecular biology techniques in pharmacology. The book offers a valuable resource for researchers in the fields of pharmacology and toxicology, as well as students pursuing a degree in or with an interest in pharmacology.

Download or read book DNA Technology in Forensic Science written by National Research Council and published by National Academies Press. This book was released on 1992-02-01 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.

Download or read book Recombinant DNA Techniques written by Monika Jain and published by Alpha Science International, Limited. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: RECOMBINANT DNA TECHNOLOGY: An Introduction has all the techniques used in the Genetic Engineering like the PCR, Microarray, transfection techniques, Blotting techniques, DNA sequencing, site directed Mutagenesis and protein engineering. Also various aspects of the gene therapy. It also have the good description of the mapping techniques along with the various molecular markers used in the mapping of the genomes like RFLP, RAPD, AFLP etc. DNA chip technology is the most important techniques used for the study of the gene expression and it is the only technique that can analyze the multiple genes at a time. This techniques is very well explained in the book. DNA sequencing by Sanger's Method and maxam and Gilbert's method is also explained by the help of good diagrams. These are the important topics covered in this book.

Download or read book Recombinant DNA Laboratory Manual written by Judith W. Zyskind and published by Elsevier. This book was released on 2014-05-12 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant DNA Laboratory Manual is a laboratory manual on the fundamentals of recombinant DNA techniques such as gel electrophoresis, in vivo mutagenesis, restriction mapping, and DNA sequencing. Procedures that are useful for studying either prokaryotes or eukaryotes are discussed, and experiments are included to teach the fundamentals of recombinant DNA technology. Hands-on computer sessions are also included to teach students how to enter and manipulate sequence information. Comprised of nine chapters, this book begins with an introduction to bacterial growth parameters, how to measure bacterial cell growth, and how to plot cell growth data. The discussion then turns to the isolation and analysis of chromosomal DNA in bacteria and Drosophila; plasmid DNA isolation and agarose gel analysis; and introduction of DNA into cells. Subsequent chapters deal with Tn5 mutagenesis of pBR329; DNA cloning in M13; DNA sequencing; and DNA gel blotting, probe preparation, hybridization, and hybrid detection. The book concludes with an analysis of lambda phage manipulations. This manual is intended for advanced undergraduate or beginning graduate students and should also be helpful to established investigators who are changing their research focus.