Download or read book Rab GTPase regulated Intracellular Trafficking and Activity of G Protein coupled Receptors written by Jennifer Lynn Seachrist and published by . This book was released on 2003 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rab GTPase regulated Intracellular Trafficking and Activity of G Protein coupled Receptors written by Jennifer Lynn Seachrist and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Trafficking Inside Cells written by Nava Segev and published by Springer Science & Business Media. This book was released on 2010-05-30 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.
Download or read book G Protein Coupled Receptors written by and published by Academic Press. This book was released on 2016-02-26 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: G-Protein-Coupled Receptors: Signaling, Trafficking, and Regulation, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in G-Protein-Coupled Receptors, and includes sections on such topics signaling, trafficking and regulation. Covers the increasingly appreciated cell biology field of G-protein-coupled receptors Includes both established and new technologies Contributed by experts in the field Covers topics such as signaling, trafficking, and regulation
Download or read book G Protein Coupled Receptors written by and published by Academic Press. This book was released on 2013-01-24 with total page 489 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high throughput assays for identification of pharmacoperone drugs. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers G protein coupled receptors Contains chapters on such topics as G protein-coupled receptor trafficking motifs, structure-based virtual screening, and automation-friendly high-throughput assays for identifying pharmacoperone drugs
Download or read book Trafficking of GPCRs written by Guangyu Wu and published by Academic Press. This book was released on 2015-06-05 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: G protein-coupled receptors (GPCRs) constitute the largest superfamily of cell surface receptors that regulate a variety of cell functions. Over the past few decades great progress has been made in defining the roles of intracellular trafficking in controlling the functionality of the receptors as well as in the development of various human diseases. This volume of Progress in Molecular Biology and Translational Science reviews the recent understanding of GPCR trafficking regulators and molecular mechanisms. Written by future leaders in the pain field Covers a wide range of targets Contains provocative ideas about GPCR trafficking
Download or read book Rab GTPases and Membrane Trafficking written by Guangpu Li and published by Bentham Science Publishers. This book was released on 2012-05-21 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Ypt/Rab GTPases form the largest branch of the Ras-related small GTPase superfamily and regulate intracellular membrane trafficking in all eukaryotes. Since their discovery over two decades ago, a wealth of information has accumulated about the roles that"
Download or read book Modulation of G Protein coupled Receptor Intracellular Trafficking and Signal Transduction written by Jessica L. Esseltine and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The seven transmembrane-spanning G Protein-coupled Receptor (GPCR) super family is the largest family of cell-surface receptors, comprising greater than 650 members. GPCRs represent the primary targets of most therapeutic drugs. Desensitization, endocytosis and recycling are major mechanisms of receptor regulation and intracellular trafficking of GPCRs is linked to the Rab family of small G proteins. In the present study, we examined whether multiple Rab GTPase regulate receptor trafficking through endosomal cellular compartments as a consequence of their direct association with GPCRs. We find that Rab4, Rab7 and Rab11 all bind to the last 10 amino acid residues of the angiotensin II Type 1 (AT1R) carboxyl-terminal tail. We show that the Rab GTPases compete with one another for receptor binding and that Rab4 effectively displaces Rab11 from the receptor. In contrast, Rab11 overexpression does not prevent Rab4 binding to the AT1R. Overexpression of wild-type Rab4, but not Rab11, facilitates AT1R dephosphorylation, and a constitutively active Rab4-Q67L mutant reduces AT1R desensitization and promotes AT1R resensitization. We also find that Rab8, a RabGTPase involved in the regulation of secretory/recycling vesicles, modulation of the actin cytoskeleton and cell polarity, interacts with the carboxyl-terminal tail of metabotropic glutamate receptor 1 (mGluR1a) and attenuates mGluR1a signalling and endocytosis in a protein kinase C-dependent manner. Finally, we have examined several previously uncharacterised but naturally occurring single nucleotide polymorphisms (SNPs) in mGluR1a that have been associated with cancer that may alter mGluR1a signalling. We find that SNPs found within the ligand binding domain of mGluR1a result in both decreased cell surface expression and basal inositol 1,4,5, trisphosphate formation and bias mGluR1a signalling via the ERK1/2 pathway. Additional mGluR1a SNPs localized to the mGluR1a glutamate binding site, intracellular regulatory domains and Homer binding site also result in changes in mGluR1a subcellular localization, signalling and cell morphology. Taken together, these results indicate that GPCR signalling is significantly modulated by the association of intracellular regulatory proteins that can be influenced by receptor structure.
Download or read book G Protein Coupled Receptors written by and published by Academic Press. This book was released on 2019-01-05 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more. Covers the increasingly appreciated cell biology field of G-protein-coupled receptors Includes both established and new technologies Contributed by experts in the field
Download or read book Rab GTPases written by Guangpu Li (Molecular biologist) and published by . This book was released on 2021 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.
Download or read book G Protein Coupled Receptors written by and published by Academic Press. This book was released on 2013-02-08 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
Download or read book ARF Family GTPases written by Richard A. Kahn and published by Springer Science & Business Media. This book was released on 2004-02-29 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: For the first time experts in the area of signalling research with a focus on the ARF family have contributed to the production of a title devoted to ARF biology. A comprehensive phylogenetic analysis of the ARF family, tables of the ARF GEFs and ARF GAPs, and more than a dozen chapters describing them in detail are provided. The impact of the ARF proteins on widely diverse aspects of cell biology and cell signalling can be clearly seen from the activities described; including membrane traffic, lipid metabolism, receptor desensitization, mouse development, microtubule dynamics, and bacterial pathogenesis. Anyone interested in understanding the complexities of cell signalling and the integration of signalling networks will benefit from this volume.
Download or read book Characterization of arrestins Trafficking and Signaling Functions on G Protein coupled Receptors written by Etienne Khoury and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "The heptahelical G protein-coupled receptors (GPCRs) are the largest, most versatile superfamily of cell surface receptors and constitute the most common target for many therapeutic drugs. They participate in various physiological processes via signaling transduction, mainly induced by heterotrimeric G proteins. Following activation, these 7-transmembrane receptors recruit [beta]-arrestin for endocytosis. Beta-arrestins will directlyassociate GPCRs to several components of the endocytic machinery, such as clathrin and adaptor protein 2 (AP-2), allowing the receptor to internalize. Over the years, [beta]-arrestins were shown to also act as signaling adaptors upon binding to agonist-occupied GPCRs; however, the mechanism regulating receptor/[beta]-arrestin complexes in endosomes was not yet addressed. Based on a previous study where we showed that [beta]-arrestin serves as a scaffolding protein for several signaling effectors (e.g. MAPK) in the endosomes, we hypothesized that endosomal MAPK activity would be necessary to maintain the GPCR/[beta]-arrestin-2 complex, thus regulating receptor trafficking. Our first study revealed a putative phosphorylating MAPK motif (Thr178) in [beta]-arrestin-2 suggesting that endosomal MAPK activity is involved in such process. Using biochemical assays and FRAP (Fluorescence Recovery After Photobleaching) approach to assess the life-time of bradykinin B2 receptor (B2R)/[beta]-arrestin-2 endosomal complexes, we showed that the MAPK putative site in [beta]-arrestin-2 is involved in regulating the association between the arrestin and its receptor. The role of [beta]-arrestin-2 'hinge' domain was tested for such effect, and using arrestin mutants demonstrated distinct behaviours between [beta]-arrestin-2 species on GPCRs trafficking and agonist mediated receptor signaling. This study highlights a strong correlation between MAPKsignaling and the regulation of endosomal GPCR/[beta]-arrestin-2 interactions. In addition to the 'hinge' domain, structural studies showed that the polar core, as well as the arrestins loops, were also crucial for [beta]-arrestin activation. Based on the crystal structure of [beta]-arrestin-1, a virtual screen was then conducted in order to identify aselective pharmacological inhibitor of [beta]-arrestin-2. Results showed a unique compound, namely UM0012685 which firstly, blocked V2-vasopressin receptor (V2R) endocytosis; Secondly, inhibited [beta]-arrestin recruitment; Thirdly, decreased the stability of the receptor and [beta]-arrestin-2 endosomal complexes and finally inhibited [beta]-arrestin-2-dependent MAPK activation upon agonist stimulation of the V2R. This compound was also used as a tool to determine [beta]-arrestins trafficking function on [beta]2-adrenergic receptor ([beta]2AR) recycling. These results uncover a better understanding of the underlying mechanism regulating [beta]-arrestin in the endosomes as well as the intracellular trafficking modulation of GPCRs. Our findings also reveal a useful tool to investigate the multifunctional aspect of [beta]-arrestins in the cell." --
Download or read book Encyclopedia of Signaling Molecules written by Sangdun Choi and published by Springer. This book was released on 2017-12-15 with total page 6330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this encyclopedia presents over 400 biologically important signaling molecules and the content is built on the core concepts of their functions along with early findings written by some of the world’s foremost experts. The molecules are described by recognized leaders in each molecule. The interactions of these single molecules in signal transduction networks will also be explored. This encyclopedia marks a new era in overview of current cellular signaling molecules for the specialist and the interested non-specialist alike. Currently, there are more than 30,000 genes in human genome. However, not all the proteins encoded by these genes work equally in order to maintain homeostasis. Understanding the important signaling molecules as completely as possible will significantly improve our research-based teaching and scientific capabilities.
Download or read book Neuromorphic Olfaction written by Krishna C. Persaud and published by CRC Press. This book was released on 2016-04-19 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many advances have been made in the last decade in the understanding of the computational principles underlying olfactory system functioning. Neuromorphic Olfaction is a collaboration among European researchers who, through NEUROCHEM (Fp7-Grant Agreement Number 216916)-a challenging and innovative European-funded project-introduce novel computing p
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Amphibian Models of Development and Disease written by and published by Academic Press. This book was released on 2021-05-30 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Amphibian Models of Development and Disease, Volume 145 in the Current Topics in Developmental Biology series, highlights new advances in the field written by an international board of experts. New chapters in this release include Building a ciliated epithelium: Transcriptional regulation and radial intercalation of multiciliated cells, Biomechanics of Amphibian Morphogenesis, Planar cell polarity during neural tube closure, Xenopus neural crest and its relevance to human disease, Endoderm organogenesis, From egg to embryo in marsupial frogs, Evo-devo lessons from the analysis of Xenopus genomes, Transcriptional regulation during zygotic genome activation, Proteomics and metabolomics for cell lineage analysis in frog embryos, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Current Topics in Developmental Biology series Includes the latest information on Amphibian Models of Development and Disease
