Download or read book Quantitative Receptor Autoradiography written by Carl A. Boast and published by . This book was released on 1986 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Receptor Binding Techniques written by Anthony P. Davenport and published by Humana. This book was released on 2005-03-25 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
Download or read book Sites of Drug Action in the Human Brain written by Anat Biegon and published by CRC Press. This book was released on 1995-02-24 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sites of Drug Action in the Human Brain uses the results of recent analyses of the regional brain distribution and binding pattern of drugs in the human brain. This new book specifically addresses drugs of abuse and treats the effects of various drugs on behavior and mood, as well as on metabolism and blood flow in the human brain. It also presents the methodological aspects of investigating the sites of drug action in the human brain. Because it focuses on the living human brain, this book differs from other books on the subject, which primarily use the results of postmortem studies. Sites of Drug Action in the Human Brain therefore provides valuable information on the clinical aspects of drug intoxification, addiction, and toxicity.
Download or read book Receptor Autoradiography written by Dr. John Wharton and published by . This book was released on 1993 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Receptor autoradiography is an invaluable technique that is widely used to localize and characterize peptide and neurotransmitter binding sites. Its extensive use reflects the utility of the technique and the important advantages it offers over conventional biochemical procedures. This instructive work makes receptor autoradiography more accessible by giving a detailed account of the methodology and its application in the analysis of receptors in the brain and peripheral organs. These examples are not exhaustive but illustrate the versatility of the technique as well as its potential pitfalls. The book provides an introduction to the receptor concept and gives detailed information on the theory and procedures involved in the autoradiographic localization and quantification of radiolabelled ligand binding sites. This volume will be an essential tool for neuroscientists, pharmacologists, histochemists, neuropathologists, researchers in the pharmaceutical industry, and students of the biological sciences.
Download or read book Handbook of Neurochemistry and Molecular Neurobiology written by Glen Baker and published by Springer Science & Business Media. This book was released on 2007-03-26 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook is intended to be a service to the neuroscience community, to help in finding available and useful information, to point out gaps in our knowledge, and to encourage continued studies. It represents the valuable contributions of the many authors of the chapters and the guidance of the editors and most important, it represents support for research in this discipline. Based on the rapid advances in the years since the second edition
Download or read book Neuroanatomical Tract Tracing Methods 2 written by Laszlo Zaborszky and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Download or read book Visualization of Receptors written by Gerard Morel and published by CRC Press. This book was released on 1997-02-26 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.
Download or read book Whole body Autoradiography written by C. G. Curtis and published by . This book was released on 1981 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Receptor Binding in Drug Research written by Robert A. O'Brien and published by CRC Press. This book was released on 2020-08-26 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the applications of receptor techniques in many different areas in addition to conventional drug and neurotransmitter binding sites. It reviews humoral modulators such as a leukotrienes, interferon, platelet-derived growth factor, and novel endogenous ligands.
Download or read book Receptors and Ligands in Neurological Disorders written by Amar K. Sen and published by Cambridge University Press. This book was released on 1988-06-02 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarises current studies which involve receptors and ligands in neurological disorders. The advance in the study of receptors in general enables investigators to explore the mechanism of action of psychotropic drugs and to minimise their side effects.
Download or read book Methods in Neurosciences written by P. Michael Conn and published by Elsevier. This book was released on 2016-07-02 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques.A convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Autoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniques**Statistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining techniques: fluoro-gold, SITS, and silver impregnation**Freezing techniques: freeze fracture and cryoprotection**Combined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques
Download or read book Receptors in the Human Nervous System written by F. A. O. Mendelsohn and published by Academic Press. This book was released on 2013-10-22 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Receptors in the Human Nervous System is a synthesis of the results of receptor mapping by leaders in the field. In addition to a comprehensive discussion of the distribution and possible interactions of the receptors of different neuroactive substances, this book also contains an abundance of pictorial representations of receptor distributions. High-quality photographs of one receptor are often juxtaposed with photographs of the distribution of a different receptor or receptor subtype for the consideration of possible interactions between different systems. The book surveys the distribution of receptor subtypes for the classical monoamine transmitters (acetylcholine, adrenaline, noradrenaline and serotonin) as well as the distribution of receptors for the excitatory and inhibitory amino acids, (glutamate, GABA and benzodiazepines) as well as the opioid peptides, angiotensen and other neuropeptides. The distribution of multiple types of serotonin receptors is given in detail, and the codistribution of receptors in the cortex is discussed. The book is directed toward researchers in the field of chemical neuroanatomy, as well as pharmacologists, neurophysiologists, and neuroscientists.
Download or read book Current Directions in Radiopharmaceutical Research and Development written by Steven J. Mather and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Radiophannaceutical research has recently undergone a major change in direction. In past years it has been concerned mainly with the development of perfusion tracers, the biodistribution of which reflect the regional blood flow to areas of major organs such as the heart and brain. However, a major new direction of interest now lies in the development of receptor-binding radio-tracers which can be used to perform in-vivo characterisation of diseased tissues and it is likely that much of the future research in this field will follow this direction. The difficulties in developing such tracers are considerable. The researcher must first identify a promising target for radiopharmaceutical development. High specific activity radioactive molecules must be designed and synthesised which will both bind to the target receptor with high affinity, and also have the physicochemical characteristics which will allow them to reach the target site in sufficient quantity while at the same time showing minimal uptake in non-target tissues. Thus the knowledge base required for radiophannaceutical development has now expanded beyond the limits of radiopharmaceutical chemistry to include aspects of biochemistry, molecular biology and conventional drug design. The portfolio of basic knowledge required to support current radiopharmaceutical development is changing and scientists working in this arena need to be trained in this regard. At the same time, the very latest developments in the field need to be communicated to the scientific community in order to stimulate the advancement of this exciting new direction of research.
Download or read book Angiotensin Receptors written by J.M. Saavedra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: From molecular biology to clinical applications of selective receptor blockade, the present volume compiles the latest advances of this emerging field. Of particular importance is the attention given to the newly discovered AT2 receptors, and the physiology and pathophysiology of angiotensin receptor subtypes. The book will provide clinicians treating cardiovascular disease and hypertension with a clearer understanding of this therapeutically important and complicated hormone.
Download or read book Handbook of Methods in Gastrointestinal Pharmacology written by Timothy S. Gaginella and published by CRC Press. This book was released on 1995-10-20 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gastroenterology has advanced through the development and application of increasingly sophisticated methods to measure changes in gastrointestinal function. Handbook of Methods in Gastrointestinal Pharmacology brings together details on commonly employed approaches in investigative gastroenterology. The book provides comprehensive coverage of methods and techniques used to investigate the mechanism of action of drugs on the GI tract. An integral part of each chapter is the discussion of development of techniques based upon physiologic mechanisms and principles in pharmacology. In vivo and in situ techniques involving whole animals, isolated tissue methodology, the use of single cell systems, and molecular biology approaches are covered. Illustrations provide a clear understanding of methodologies discussed. Emphasis is placed on advantages and disadvantages of each technique in answering specific research questions. Chapters are written by experts experienced in the techniques they discuss; many pioneered one or more widely used methods. The wide variety of topics included make the Handbook of Methods in Gastrointestinal Pharmacology useful to established investigators, research fellows, and graduate students. Additionally, reviewers of grants and manuscripts can use it to clarify questions that arise regarding appropriate use of a technique in a particular setting.
Download or read book The Neurobiological Basis of Suicide written by Yogesh Dwivedi and published by CRC Press. This book was released on 2012-06-25 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: With recent studies using genetic, epigenetic, and other molecular and neurochemical approaches, a new era has begun in understanding pathophysiology of suicide. Emerging evidence suggests that neurobiological factors are not only critical in providing potential risk factors but also provide a promising approach to develop more effective treatment and prevention strategies. The Neurobiological Basis of Suicide discusses the most recent findings in suicide neurobiology. Psychological, psychosocial, and cultural factors are important in determining the risk factors for suicide; however, they offer weak prediction and can be of little clinical use. Interestingly, cognitive characteristics are different among depressed suicidal and depressed nonsuicidal subjects, and could be involved in the development of suicidal behavior. The characterization of the neurobiological basis of suicide is in delineating the risk factors associated with suicide. The Neurobiological Basis of Suicide focuses on how and why these neurobiological factors are crucial in the pathogenic mechanisms of suicidal behavior and how these findings can be transformed into potential therapeutic applications.
Download or read book Molecular Neurobiology of the Olfactory System written by F.L. Margolis and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The sense of smell and the olfactory system have been a subject of intrinsic interest for millenia. Inquiry into the structure and function of the olfactory system is based on a long tradition that dates back at least to the ancient Greeks. The mechanistic basis for the sensitivity and selectivity of this chemosensory detection system has always posed a challenge and remained largely a mystery. Recently, there has been a renaissance of interest in it and especially in the application of contemporary techniques of biochemistry and cellular and molecular biology. In this volume, current research utilizing these ap proaches is discussed in depth by a group of scientists who are among the current leaders in the applications of these techniques to the olfactory system. These authors address a wide range of questions that bear directly on the olfactory system but have broader biological implications as well. The various chapters have been grouped into five broad subject areas that emphasize diverse but related questions. "Transduction and Ligand-Receptor Interactions" considers the biochemical bases of stimulus access, interaction, transduction, elimination, and information processing.
