Download or read book Quantitative Imaging in Cell Biology written by and published by Academic Press. This book was released on 2014-06-25 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Download or read book Coherent Light Microscopy written by Pietro Ferraro and published by Springer Science & Business Media. This book was released on 2011-02-09 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Download or read book Microstructure of Dairy Products written by Mamdouh El-Bakry and published by John Wiley & Sons. This book was released on 2018-07-13 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the most recent developments in microscopy techniques and types of analysis used to study the microstructure of dairy products This comprehensive and timely text focuses on the microstructure analyses of dairy products as well as on detailed microstructural aspects of them. Featuring contributions from a global team of experts, it offers great insight into the understanding of different phenomena that relate to the functional and biochemical changes during processing and subsequent storage. Structured into two parts, Microstructure of Dairy Products begins with an overview of microscopy techniques and software used for microstructural analyses. It discusses, in detail, different types of the following techniques, such as: light microscopy (including bright field, polarized, and confocal scanning laser microscopy) and electron microscopy (mainly scanning and transmission electron microscopy). The description of these techniques also includes the staining procedures and sample preparation methods developed. Emerging microscopy techniques are also covered, reflecting the latest advances in this field. Part 2 of the book focuses on the microstructure of various dairy foods, dividing each into sections related to the microstructure of milk, cheeses, yogurts, powders, and fat products, ice cream and frozen dairy desserts, dairy powders and selected traditional Indian dairy products. In addition, there is a review of the localization of microorganism within the microstructure of various dairy products. The last chapter discusses the challenges and future trends of the microstructure of dairy products. Presents complete coverage of the latest developments in dairy product microscopy techniques Details the use of microscopy techniques in structural analysis An essential purchase for companies, researchers, and other professionals in the dairy sector Microstructure of Dairy Products is an excellent resource for food scientists, technologists, and chemists—and physicists, rheologists, and microscopists—who deal in dairy products.

Download or read book Bioimage Data Analysis Workflows written by Kota Miura and published by Springer Nature. This book was released on 2019-10-17 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. It refrains from focusing on theory, and instead uses practical examples and step-by step protocols to familiarize readers with the most commonly used image processing and analysis platforms such as ImageJ, MatLab and Python. Besides gaining knowhow on algorithm usage, readers will learn how to create an analysis pipeline by scripting language; these skills are important in order to document reproducible image analysis workflows. The textbook is chiefly intended for advanced undergraduates in the life sciences and biomedicine without a theoretical background in data analysis, as well as for postdocs, staff scientists and faculty members who need to perform regular quantitative analyses of microscopy images.

Download or read book Imaging Cellular and Molecular Biological Functions written by Spencer L. Shorte and published by Springer Science & Business Media. This book was released on 2007-09-12 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Download or read book Digital Microscopy written by Greenfield Sluder and published by Elsevier. This book was released on 2007-04-26 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. - Expands coverage to include discussion of confocal microscopy not found in the previous edition - Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Download or read book Computer Vision for Microscopy Image Analysis written by Mei Chen and published by Academic Press. This book was released on 2020-12-01 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: Are you a computer scientist working on image analysis? Are you a biologist seeking tools to process the microscopy data from image-based experiments? Computer Vision for Microscopy Image Analysis provides a comprehensive and in-depth discussion of modern computer vision techniques, in particular deep learning, for microscopy image analysis that will advance your efforts. Progress in imaging techniques has enabled the acquisition of large volumes of microscopy data and made it possible to conduct large-scale, image-based experiments for biomedical discovery. The main challenge and bottleneck in such experiments is the conversion of "big visual data" into interpretable information. Visual analysis of large-scale microscopy data is a daunting task. Computer vision has the potential to automate this task. One key advantage is that computers perform analysis more reproducibly and less subjectively than human annotators. Moreover, high-throughput microscopy calls for effective and efficient techniques as there are not enough human resources to advance science by manual annotation. This book articulates the strong need for biologists and computer vision experts to collaborate to overcome the limits of human visual perception, and devotes a chapter each to the major steps in analyzing microscopy images, such as detection and segmentation, classification, tracking, and event detection. Discover how computer vision can automate and enhance the human assessment of microscopy images for discovery Grasp the state-of-the-art approaches, especially deep neural networks Learn where to obtain open-source datasets and software to jumpstart his or her own investigation

Download or read book Quantitative Phase Imaging of Cells and Tissues written by Gabriel Popescu and published by McGraw Hill Professional. This book was released on 2011-03-14 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI

Download or read book Microscope Image Processing written by Qiang Wu and published by Elsevier. This book was released on 2010-07-27 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: Digital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology. Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and display, object measurement and classification, autofocusing, and structured illumination. Key Features: - Detailed descriptions of many leading-edge methods and algorithms - In-depth analysis of the method and experimental results, taken from real-life examples - Emphasis on computational and algorithmic aspects of microscope image processing - Advanced material on geometric, morphological, and wavelet image processing, fluorescence, three-dimensional and time-lapse microscopy, microscope image enhancement, MultiSpectral imaging, and image data management This book is of interest to all scientists, engineers, clinicians, post-graduate fellows, and graduate students working in the fields of biology, medicine, chemistry, pharmacology, and other related fields. Anyone who uses microscopes in their work and needs to understand the methodologies and capabilities of the latest digital image processing techniques will find this book invaluable. - Presents a unique practical perspective of state-of-the-art microcope image processing and the development of specialized algorithms - Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments - Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject

Download or read book Principles And Advanced Methods In Medical Imaging And Image Analysis written by Atam P Dhawan and published by World Scientific. This book was released on 2008-03-17 with total page 869 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computerized medical imaging and image analysis have been the central focus in diagnostic radiology. They provide revolutionalizing tools for the visualization of physiology as well as the understanding and quantitative measurement of physiological parameters. This book offers in-depth knowledge of medical imaging instrumentation and techniques as well as multidimensional image analysis and classification methods for research, education, and applications in computer-aided diagnostic radiology. Internationally renowned researchers and experts in their respective areas provide detailed descriptions of the basic foundation as well as the most recent developments in medical imaging, thus helping readers to understand theoretical and advanced concepts for important research and clinical applications.

Download or read book Scanning Electron Microscopy and X Ray Microanalysis written by Joseph Goldstein and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has evolved by processes of selection and expansion from its predecessor, Practical Scanning Electron Microscopy (PSEM), published by Plenum Press in 1975. The interaction of the authors with students at the Short Course on Scanning Electron Microscopy and X-Ray Microanalysis held annually at Lehigh University has helped greatly in developing this textbook. The material has been chosen to provide a student with a general introduction to the techniques of scanning electron microscopy and x-ray microanalysis suitable for application in such fields as biology, geology, solid state physics, and materials science. Following the format of PSEM, this book gives the student a basic knowledge of (1) the user-controlled functions of the electron optics of the scanning electron microscope and electron microprobe, (2) the characteristics of electron-beam-sample inter actions, (3) image formation and interpretation, (4) x-ray spectrometry, and (5) quantitative x-ray microanalysis. Each of these topics has been updated and in most cases expanded over the material presented in PSEM in order to give the reader sufficient coverage to understand these topics and apply the information in the laboratory. Throughout the text, we have attempted to emphasize practical aspects of the techniques, describing those instru ment parameters which the microscopist can and must manipulate to obtain optimum information from the specimen. Certain areas in particular have been expanded in response to their increasing importance in the SEM field. Thus energy-dispersive x-ray spectrometry, which has undergone a tremendous surge in growth, is treated in substantial detail.

Download or read book Second Harmonic Generation Imaging written by Francesco S. Pavone and published by Taylor & Francis. This book was released on 2016-04-19 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Download or read book Biomedical Image Analysis written by Rangaraj M. Rangayyan and published by CRC Press. This book was released on 2004-12-30 with total page 1312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computers have become an integral part of medical imaging systems and are used for everything from data acquisition and image generation to image display and analysis. As the scope and complexity of imaging technology steadily increase, more advanced techniques are required to solve the emerging challenges. Biomedical Image Analysis demonstr

Download or read book Scanning Transmission Electron Microscopy written by Stephen J. Pennycook and published by Springer Science & Business Media. This book was released on 2011-03-24 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning transmission electron microscopy has become a mainstream technique for imaging and analysis at atomic resolution and sensitivity, and the authors of this book are widely credited with bringing the field to its present popularity. Scanning Transmission Electron Microscopy(STEM): Imaging and Analysis will provide a comprehensive explanation of the theory and practice of STEM from introductory to advanced levels, covering the instrument, image formation and scattering theory, and definition and measurement of resolution for both imaging and analysis. The authors will present examples of the use of combined imaging and spectroscopy for solving materials problems in a variety of fields, including condensed matter physics, materials science, catalysis, biology, and nanoscience. Therefore this will be a comprehensive reference for those working in applied fields wishing to use the technique, for graduate students learning microscopy for the first time, and for specialists in other fields of microscopy.

Download or read book Image Analysis and Recognition written by Aurélio Campilho and published by Springer. This book was released on 2016-07-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the thoroughly refereed proceedings of the 13th International Conference on Image Analysis and Recognition, ICIAR 2016, held in Póvoa de Varzim, Portugal, in July 2016. The 79 revised full papers and 10 short papers presented were carefully reviewed and selected from 167 submissions. The papers are organized in the following topical sections: Advances in Data Analytics and Pattern Recognition with Applications, Image Enhancement and Restoration, Image Quality Assessment, Image Segmentation, Pattern Analysis and Recognition, Feature Extraction, Detection and Recognition, Matching, Motion and Tracking, 3D Computer Vision, RGB-D Camera Applications, Visual Perception in Robotics, Biometrics, Biomedical Imaging, Brain Imaging, Cardiovascular Image Analysis, Image Analysis in Ophthalmology, Document Analysis, Applications, and Obituaries. The chapter 'Morphological Separation of Clustered Nuclei in Histological Images' is published open access under a CC BY 4.0 license at link.springer.com.

Download or read book Backscattered Scanning Electron Microscopy and Image Analysis of Sediments and Sedimentary Rocks written by David H. Krinsley and published by Cambridge University Press. This book was released on 2005-09-15 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: Backscattered scanning electron microscopy (BSE) reveals the minerals, textures, and fabrics of sediments and rocks in much greater detail than is possible with conventional optical microscopy. Backscattered Scanning Electron Microscopy provides a concise summary of the BSE technique. This comprehensive guide uses abundant images to illustrate the type of information BSE yields and the application of the technique to the study of sediments and sedimentary rocks. The authors review the use of this petrographic technique on all the major sedimentary rock types, including sediment grains, sandstones, shales, carbonate rocks, rock varnish, and glauconite. They also describe image analysis techniques that allow quantification of backscattered scanning electron microscope images. Heavily illustrated and lucidly written, this book will provide researchers and graduate students with the most current research on this important geological tool.

Download or read book Stimulated Raman Scattering Microscopy written by Ji-Xin Cheng and published by Elsevier. This book was released on 2021-12-04 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. - Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy - Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics - Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics