Download or read book Quantitive Analysis Of Biospec written by Per Lundahl and published by Routledge. This book was released on 2021-09-01 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.
Download or read book High throughput Study of Biospecific Interactions with Surface Plasmon Resonance Imaging written by Zhuangzhi Wang and published by . This book was released on 2007 with total page 380 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Download or read book Protein Dye Interactions Developments and Applications written by M.A. Vijayalakshmi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the papers and reports presented at the First International Conference on Dye-Protein Interaction, held 24-28 July 1988 at the University of Compiegne, France. This was the first international meeting dealing entirely with dye-protein interaction. The major focus of the conference was on the better understanding of the mechanism of interaction of proteins with different triazine dyes and the synthesis of novel structural dyes having good biomimetic activities. The potentials and limits of their use in biotechnology, mainly for purification, were stressed. Current contributions in developing dye-based affinity methods were highlighted in such areas as affinity partition, affinity precipitation and new support matrices for efficient affinity chromatography, etc. The interrelation between metal chelates and dyes in terms of their interactions with proteins was underlined. It is our belief that this proceedings volume will be a stimulus for broad and creative applications of dye affinity concepts in many fields of biomedical research and biotechnology. for understanding the In addition, a discussion session emphasised the necessity toxicological aspects of these dyes, their fragments and their metabolites. This helped to trigger plans for future work, and this topic will be one of the priorities in a future meeting on dye-protein interactions. C. R. The help of the International Scientific Committee, which included Drs Lowe (UK), G. KopperschHiger (GDR) , E. Stellwagen (USA), D. Thomas (France), G. Birkenmeier (GDR), S. Rajgopal-Narayan (USA), J. P. Dandeu (France), D. Muller (France) and E. Dellacherie (France), in organising this meeting is gratefully acknowledged.
Download or read book Biomolecular Sensors written by Electra Gizeli and published by CRC Press. This book was released on 2002-05-16 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of devices that incorporate biological assemblies is impacting analytical and biomedical research. Today, scientists can monitor vital biological interactions-such as the binding of DNA to proteins-in real time, deriving unique information necessary to understanding biochemical pathways and thus aiding the design of drugs to regulat
Download or read book Carbohydrate Analysis by Modern Liquid Phase Separation Techniques written by Ziad El Rassi and published by Elsevier. This book was released on 2021-08-15 with total page 956 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Download or read book Soybean written by Aleksandra Sudarić and published by BoD – Books on Demand. This book was released on 2011-04-11 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book Soybean: Molecular Aspects of Breeding focuses on recent progress in our understanding of the genetics and molecular biology of soybean and provides a broad review of the subject, from genome diversity to transformation and integration of desired genes using current technologies. This book is divided into four parts (Molecular Biology and Biotechnology, Breeding for Abiotic Stress, Breeding for Biotic Stress, Recent Technology) and contains 22 chapters.
Download or read book Quantitative Methods and Analytical Techniques in Food Microbiology written by Leonardo Sepúlveda Torre and published by CRC Press. This book was released on 2022-06-09 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides up-to-date and detailed scientific information on recent developments and new approaches in food microbiology, focusing on microbial food pathogens. The volume presents the fundamental aspects of food and microorganisms, and also addresses food systems and measures to prevent and control food, foodborne diseases, etc. According to the editors, every minute, there are about 50,000 cases of gastrointestinal diseases from food-mediated infections and food poisoning, and many individuals, especially children, die from these infections. The most important preventive measures are for the development and continuous implementation of effective interventions to improve overall food safety. The book helps to meet the challenge of food safety issues by focusing on the fundamental aspects of food and microorganisms. Each section consists of detailed information on the particular aspects of each topic, including basic microbiology, safety, pathogenic microorganisms, food conservation, sanitization, and hygiene procedures. The microbial diversity found in food is described from the classification by kingdoms and the main groups of microorganisms present in them. Although the main issue is microbial food pathogens, the book also covers another important aspect of food microbiology: food systems and measurements to prevent and control food, foodborne diseases, etc. Quantitative Methods Quantitative Methods and Analytical Techniques in Food Microbiology: Challenges and Health Implications will be a valuable resource for scientists, researchers, faculty, students, and others in various sectors in food science and technology. The scope of food microbiology is highly inclusive, as it interacts with all subdisciplines of microbiology, such as public health microbiology, microbial genetics, fermentation technologies, microbial physiology and biochemistry, and food microbiologists have been at the forefront of many microbiological concepts and advances.
Download or read book Protein Interactions written by Peter Schuck and published by Springer Science & Business Media. This book was released on 2007-03-20 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.
Download or read book Electrochemical Sensing Carcinogens in Beverages written by Asif Iqbal Zia and published by Springer. This book was released on 2016-05-09 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes a robust, low-cost electrochemical sensing system that is able to detect hormones and phthalates – the most ubiquitous endocrine disruptor compounds – in beverages and is sufficiently flexible to be readily coupled with any existing chemical or biochemical sensing system. A novel type of silicon substrate-based smart interdigital transducer, developed using MEMS semiconductor fabrication technology, is employed in conjunction with electrochemical impedance spectroscopy to allow real-time detection and analysis. Furthermore, the presented interdigital capacitive sensor design offers a sufficient penetration depth of the fringing electric field to permit bulk sample testing. The authors address all aspects of the development of the system and fully explain its benefits. The book will be of wide interest to engineers, scientists, and researchers working in the fields of physical electrochemistry and biochemistry at the undergraduate, postgraduate, and research levels. It will also be highly relevant for practitioners and researchers involved in the development of electromagnetic sensors.
Download or read book Protein Microarray Technology written by Dev Kambhampati and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the first of its kind in the field of protein microarrays and addresses novel strategies for constructing highly functional and biocompatible microarrays for screening proteins. The list of authors consisting of world leading experts provide a roadmap for solving the complex challenges that are currently faced while monitoring protein-protein interactions over a wide range of microarray platforms. In doing so, they also offer a comprehensive overview of microarray surface chemistry, detection technologies, fabrication options for array development, and data analysis of numerous types of protein interactions. Topics covered include: -Types of biomolecular interactions -Surface chemistry -Detection technologies -Spotting technologies -Bioinformatics/data analysis. While primarily intended to serve as a reference for researchers and students embarking on the exciting fields of proteomics, drug discovery and clinical diagnostics, this technology is also expected to potentially impact the areas of food diagnostics, environmental monitoring and national security.
Download or read book Mathematical Models and Immune Cell Biology written by Carmen Molina-París and published by Springer Science & Business Media. This book was released on 2011-05-05 with total page 413 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whole new areas of immunological research are emerging from the analysis of experimental data, going beyond statistics and parameter estimation into what an applied mathematician would recognise as modelling of dynamical systems. Stochastic methods are increasingly important, because stochastic models are closer to the Brownian reality of the cellular and sub-cellular world.
Download or read book Visualization of Receptors written by Gerard Morel and published by CRC Press. This book was released on 1997-02-26 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.
Download or read book Electrospray and MALDI Mass Spectrometry written by Richard B. Cole and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Download or read book Nanofabrication and Biosystems written by Harvey C. Hoch and published by Cambridge University Press. This book was released on 1996-07-13 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book also aims to stimulate innovative, productive interactions among materials scientists, engineers, and biologists, and to explore ways in which materials scientists and engineers can exploit biological principles and biological assemblies to produce new and ever smaller devices.
Download or read book HIV Protocols written by Nelson Michael and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt: The worldwide impact of infection with human immunodeficiency virus type 1 (HIV- is reflected in the cumulative number ofHIV- 1 infections, which is now predicted to exceed 40 million by the year 2000---equivalent to the n- ber of humans who perished in World War II. The medical and scientific - sponse to the HIV-1 pandemic has steadily grown since its recognition in 1981. The outlay by the United States alone for HIV research funded by the National Institutes of Health in 1997 was $1. 4 billion. Laboratory-based HIV research has brought together academic clinicians, retrovirologists, molecular biologists, and immunologists in the formation of research teams attempting to dissect the viral and host factors contributing to disease pathogenesis. Increasing focus is being placed on those aspects of viral biology and host immune responses that bear on the development of vaccines to prevent HIV infection. HIVProtocols reflects the state of HIV research in several ways. First, chapters are organized into four sections: Virology, Molecular Biology, Humoral Immunology, and Cellular Immunology. This organization is a natural consequence of the diverse scientific disciplines that have been attracted to HIV research. Second, the chapters reflect such diverse research directions as viral coreceptor usage, quantitation of viral genomes, HIV promoter function, B-cell epitope mapping, and measurements of T-cell function, each of which bears on the goal of understanding the viral and host immune responses that will be critical to the design of effective preventive vaccines.
Download or read book Glyconanotechnology written by Tomáš Bertók and published by CRC Press. This book was released on 2019-11-08 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Glycomics (a comprehensive study of glycans, often denoted as the third alphabet of molecular biology, besides nucleic acids and proteins) relies on techniques that use "heavy machinery", such as mass spectrometry, liquid chromatography, and capillary electrophoresis. The use of biosensors and biochips for glycan analysis offers the possibility of lower costs, lower time and sample consumption, and ease of use. This highly interdisciplinary book introduces students, researchers, and professionals to the broad scope of this area, including the basics of nanotechnology and materials science, glycomics, and bioanalytical chemistry, as well as several historical facts, contexts and future perspectives. The introductory chapters are utilized in the book later for the state-of-the-art chapters on glycan-based diagnostics and imaging, enrichment techniques, and therapy, which makes this book unique and of great interest to a wide audience. The authors are skilled specialists and research scientists with many years of experience in this area and former investigators on a prestigious European Research Council Starting Grant.
