Download or read book Qualitative and Quantitative Liquid Chromatography and Capillary Electrophoresis tandem Mass Spectrometry for Drug Metabolism Studies and Ultra Trace Analysis written by Jianyi Cai and published by . This book was released on 1996 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dissertation Abstracts International written by and published by . This book was released on 1996 with total page 1006 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Coupling Liquid Chromatography to Capillary Zone Electrophoresis Tandem Mass Spectrometry for Deep Top down Proteomics written by Elijah Neal McCool and published by . This book was released on 2021 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomes are very complex with a large number of unique proteoforms spread across a wide concentration dynamic range. This means that an MS-based platform with highly efficient separation and highly sensitive detection of proteoforms is required. Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been suggested as one such platform. When coupled to offline liquid chromatography-based fractionation, CZE-MS/MS has proven to be invaluable to the TDP community.In Chapter 2, the first optimization of dynamic pH junction-based sample stacking for TDP is provided along with one of the first comparisons of reversed-phase liquid chromatography coupled to mass spectrometry (RPLC-MS) and CZE-MS/MS. Optimization of dynamic pH junction is performed with a standard protein mixture, and this platform was ultimately applied to an Eschericia coli (E. coli) whole cell lysate. This resulted in the largest TDP dataset for single-shot CZE-MS/MS. The comparison of RPLC-MS/MS and CZE-MS/MS also included analysis of an E. coli cell lysate and resulted in high numbers of identifications and highlighted the various pros and cons of each method.In Chapter 3, two dimensional LC fractionation (size exclusion chromatography (SEC) and RPLC) was coupled to CZE-MS/MS for deep TDP of E. coli cells. This study resulted in the largest TDP dataset, at the time, for E. coli, identifying 5700 proteoforms and 850 proteins. We were also able to identify and localize various interesting PTMs and estimate protein abundances using a spectral counting method. From this study it was clear thatour platform was comparable to other RPLC-MS/MS methods for deep TDP in terms of number of proteoform identifications and total instrument time.In Chapter 4, we applied our TDP platform to two isogenic colorectal cancer (CRC) cell lines, SW480 and SW620, from primary and metastatic tumors. Genetic changes have been known for a long time to affect CRC progression but this was the first proteoform-level deep TDP study of CRC metastasis. In total, we identified over 23000 proteoforms and over 2000 proteins, for the largest TDP dataset of any cell type and was a 400% increase in terms of identifications over previous deep TDP studies. We used a special database searching tool to identify single amino acid variants (SAAVs) for the largest dataset of proteoforms containing SAAVs. Quantitative analysis identified 460 proteoforms with significant differences in abundance between SW480 and SW620. Several of these proteoforms were also phosphorylated which could further impact disease progression and outcome for a specific patient phenotype and could serve as biomarkers for deciding how to treat a patient or for drug development.In Chapter 5, both activated ion electron transfer dissociation (AI-ETD) and ultraviolet photodissociation (UVPD) at 213 nm were coupled to CZE for deep TDP of E. coli and zebrafish brain samples, respectively. Optimized CZE-AI-ETD and CZE-UVPD resulted in large numbers of proteoform identifications, and many important modifications were identified and localized using these effective fragmentation techniques. This included N-terminal acetylation, methylation, S-thiolation, disulfide bonds, and lysine succinylation.In Chapter 6, a variety of insights into the future of TDP are provided. This includes important applications for TDP, such as personalized medicine, drug development, embryonic development, and pathogen identification. Also, a few advancements to the TDP workflow that may have increased focus on in the future are mentioned.

Download or read book The Analysis of Biological Samples Using Capillary Electrophoresis and HPLC Technologies written by and published by . This book was released on 2007 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) are two very important separation techniques. CE is a microseparation technique in which an electric field is applied to perform the separation. In CE, charged species are separated based on their charge/mass ratio, making this technique useful for separating a wide variety of components, including those found in biological samples. HPLC is the most widely used separation technique due to the ease of use and wide variety of components that can be separated. HPLC employs the use of pressure to push mobile phase through an analytical column containing a stationary phase that interacts with the solutes to effect the separation. Both CE and HPLC can use a number of different detection schemes to monitor the separated species, including ultra-violet (UV), laser-induced fluorescence (LIF), and mass spectrometry (MS). Affinity capillary electrophoresis (ACE) combines the separation power of CE with the selectivity of immunoassays. A fluorescein labeled estradiol derivative was evaluated for potential use as a competitive inhibitor in ACE. The labeling procedure rendered multiple components with the fluorescein label. Two of the components were identified as active estradiol species against the anti-estradiol antibody, examined by CE-LIF. Since only one active species had been predicted, both species were characterized using CE-MS and MS/MS, in addition to testing them in the ACE format. Nucleoside reverse transcriptase inhibitors (NRTIs) are one class of anti-HIV (human immunodeficiency virus) drugs. Because HIV is considered a pandemic, the analysis of NRTIs in human samples is very important; people all over the world are using these drugs. Currently, multiple NRTIs are given for treatment; however, there are few methods to date that separate and detect multiple NRTIs in a single run. NRTIs are metabolized in the body into their active triphosphate form. Two approaches can be followed for their separation and detection. The first approach was an indirect method, in which the active triphosphate form was dephosphorylated into the parent form of the NRTIs. HPLC with UV detection was utilized to establish an optimized separation method prior to using LC-MS/MS for the analysis of the following NRTIs in human peripheral blood monunclear cells (PBMCs): stavudine (D4T), lamivudine (3TC), the metabolized form of didanosine (ddA), zidovudine (ZDV), and the internal standard. The second approach was a direct method, in which analysis of the triphosphate form of the NRTIs was performed without dephosphorylation of the drugs. A capillary eletrophoretic method was developed that allows separation of the triphosphate NRTIs of interest: zidovudine triphosphate (ZDV-TP), stavudine triphosphate (D4T-TP), lamivudine triphosphate (3TC-TP), 2',3'-dideoxyadenoine triphosphate (ddA-TP), tenofivir diphosphate (TFV-DP), and emtricitabine triphosphate (FTC-TP). The separation was optimized using UV detection. Since UV detection does not offer appropriate detection limits, we studied the native fluorescence spectroscopic characteristics of the triphosphate NRTIs. We constructed an in-house CE-LIF system to explore LIF detection of the phosphorylated NRTIs. The system employed the use of a deep-UV laser with excitation at 224 nm. A sheath-flow cuvette was integrated into the design to improve detectability. The CE-LIF system was aligned and tested using the native fluorescence of tryptophan. The CE-LIF system was then used for the analysis of the active metabolized forms of NRTIs. With this system, we were able to separate and detect ZDV-TP, D4T-TP, 3TC-TP, ddA-TP, and the internal standard using a modification of the separation conditions previously optimized with UV detection. The detection limits obtained are not sufficiently low for the analysis of human PBMCs.

Download or read book Liquid Chromatography and Capillary Electrophoresis Methodologies for the Analysis of Biological Samples written by and published by . This book was released on 2008 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation techniques such as capillary electrophoresis and liquid chromatography have found application in the analysis of biological samples. In capillary electrophoresis charged species are separated based on their mass to charge ratios under the influence of an applied electric field. In liquid chromatography, analytes are separated based on their partition between a stationary and mobile phase. Separation techniques when combined with a variety of detection techniques such as ultraviolet, laser induced fluorescence, mass spectrometry and electrochemical detection have been very successful in the quantification of analytes comprised in complex mixtures (e.g., biological samples). Capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection was used to separate different bioconjugated CdSe/ZnS quantum dots (QDs). The QD nanocrystals studied were conjugated to the biomolecules streptavidin, biotin, and immunoglobulin G. The bioconjugated QDs showed different electrophoretic mobilities, which appear to depend upon the biomolecule that is attached to the QD and the buffer solution used. The use of a polymeric additive into the CE run buffer improved the resolution of the bioconjugates. Under CE conditions, the interaction between QD bioconjugates containing streptavidin (QDSt) and biotin (QDBi) was monitored. In a two color experiment, two different sizes of QD (i.e., 585 nm and 655 nm) were used to monitor the interaction between 655 nm QDBi and 585 nm QDSt. The use of QDs with different emission properties allows the selective monitoring of two different wavelengths while using one single excitation source. This in turn, allowed the monitoring of overlapping peaks in the electropherogram when newly formed products resulting from the interaction of the two bioconjugated QDs appeared. Protease inhibitors (PIs) are a class of compounds that are used in the treatment of patients with the human immunodeficiency virus (HIV). These drugs inhibit the HIV-1 protease, acting on the production of viral proteins essential for the completion of the viral replication process. Monitoring free drug concentration is necessary to establish the dosage that would result in optimum free drug concentration in blood for a given patient. We developed methodology to analyze free protease inhibitor lopinavir in plasma samples. Ultrafiltration was used to isolate the free drug; then LC/MS/MS was used for sample analysis. Restrictive access media columns have been developed to simplify sample preparation processes. These columns allow the direct injection of untreated biological samples. Restrictive access media columns exclude macromolecules such as proteins, while retaining small molecules such as drugs. Two restrictive access material columns were evaluated for the direct injection of plasma for analysis of the protease inhibitor lopinavir. In the columns studied the interaction of plasma components with the material inside the column was more than expected. Glucose monitoring and insulin therapy are becoming a standard of care in patients in the intensive care unit. In order to control glycemic levels, frequent glucose monitoring is required. The use of tear fluid glucose concentrations was evaluated as a non-invasive sampling strategy for monitoring glucose in intensive care unit patients. Glucose in tear fluid samples from intensive care unit patients were analyzed using anion exchange chromatography with pulsed amperometric detection. The results did not show a correlation between tear fluid and blood glucose concentrations.

Download or read book Electrospray and MALDI Mass Spectrometry written by Richard B. Cole and published by John Wiley & Sons. This book was released on 2011-09-26 with total page 900 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1995 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Quantitative Analysis of Multiply sic Charged Large Molecules in Human Or Rat Plasma Using Liquid Chromatography Tandem Mass Spectrometry written by Matthew Sean Halquist and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassays have traditionally been employed for the determination of plasma concentration-time profiles for pharmacokinetic studies of therapeutic proteins and peptides. These ligand binding assays have high sensitivity but require significant time for antibody generation (1 to 2 years) for assay development. Despite high sensitivity, these assays suffer from cross-reactivity that can lead to inaccurate results. As an alternative to immunoassays, this dissertation was focused on the development and validation of assays that can be used for quantitative analysis of peptides or proteins in plasma using liquid chromatography tandem mass spectrometry (LC-M.S./MS). Two approaches were considered for measurement of proteins and peptides fortified in plasma. The first approach involved employing signature peptides as quantitative surrogates of a target protein. This approach is a multistep process that includes: computer simulated (in silico) peptide predictions, protein purification, proteolytic digestion, peptide purification, and ultimately mass spectrometry. Signature peptides were determined through in silico peptide predictions and iterative tuning processes to represent Amevive℗' (Alefacept), a therapeutic for psoriasis, for quantification in human plasma. Horse heart myoglobin was chosen as a protein analogue internal standard to compensate for errors associated with matrix effects and to track recovery throughout the entire sample pretreatment process. Samples were prepared for analysis by selective precipitation of the target proteins with optimized pH and heat conditions followed by enzymatic digestion, dilution, and filtration. Combining selective precipitation and protein analogue internal standard lead to a method validated according to current FDA guidelines and achieved a linear range (250-10,000 ng/mL) suitable for monitoring the therapeutic levels of Alefacept (500 -6000 ng/mL) without the use of antibodies. A second approach exploited the mass spectrometric behavior of intact polypeptides. A polypeptide can exist in multiple charge states separated by mass to charge ratio (m/z). Herein, the charge state distribution and the formation of product ions to form selected reaction monitoring (SRM) transitions for intact polypeptide quantitative analysis was evaluated in plasma. Oxyntomodulin, a 37 amino acid anorectic peptide (4449 Da), was employed as a model for analysis in rat plasma. The +7 charge state form of OXM was used to form an SRM for quantitative analysis. Two-dimensional reversed phase ion pair chromatography, a modified solid phase extraction, and a multiply charged SRM of oxyntomodulin enabled a lower limit of quantification of 1 ng/mL. Following development of the LC-MS/MS method, a validation of this approach was performed according to FDA guidelines. Finally, to show further utility of LC-MS/MS, the validated oxyntomodulin method was used in a pharmacokinetic study with Sprague-Dawley rats. Rats were dosed with oxyntomodulin through intravenous or intratracheal instillation routes of administration. Plasma concentration-time profiles were determined. Using these profiles, noncompartmental parameters were determined for each dose and routes of administration.

Download or read book High Performance Liquid Chromatography Tandem Mass Spectrometry for Trace Analysis in Environmental Samples written by Heather L. Fleming and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: 1,3-Dimethylamylamine (1,3-DMAA) is a stimulant commercially sold in a variety of dietary supplements as a chemical species derived from geranium plants (Pelargonium graveolens). Whether 1,3-DMAA naturally occurs in geranium plants or other dietary ingredients, it has important regulatory and commercial ramifications. An extraction method combined with HPLC-MS/MS is used to determine 1,3-DMAA and 1,4-dimethylamylamine (1,4-DMAA) concentrations in geranium plants with both external calibration and standard addition methods. Samples from the Changzhou, Kunming, and Guiyang regions of China during both winter and summer were analyzed. Following the detection of DMAA in the Changzhou sample, an extraction and pre-column, chiral derivatization chemistry method was developed for the separation and analysis of the four stereoisomers of 1,3-DMAA and two enantiomers for 1,4-DMAA. Two chiral derivatizing agents (CDAs) were investigated: ( - )-1-(9-fluorenyl)ethyl chloroformate [( - )-FLEC] and (R)-( - )-alpha-methoxy-alpha-(trifluoromethyl) phenylacetyl [( - )-MTPA]. Optimization studies and detailed method detection limit (MDL), accuracy, and precision and linearity studies are presented for analysis of the DMAA-FLEC species in geranium plants. The DMAA-FLEC product was found to be unstable and a second, more stable CDA [( - )-MTPA] was employed. A preparatory scale HPLC separation was added prior to derivatization with ( - )-MTPA to aid in the separation of all DMAA stereoisomers. The propagation of error prohibited a confident analysis of individual DMAA stereoisomers, but the DMAA-FLEC and DMAA-MTPA methods both confirmed the presence of DMAA in the Changzhou plant samples. Haloacetic acids (HAAs) are formed during the chlorination of drinking water. Previous work detected HAAs in bulk sodium hypochlorite solutions with post column reaction ion chromatography (PCR-IC). A HPLC-MS/MS method was designed to provide confirmation of the presence of HAAs in the sodium hypochlorite solutions. Detailed MDL, accuracy, and precision studies are presented for the analysis of nine haloacetic acids in sodium hypochlorite solutions. Due to the complex nature of the sample matrix a solid phase extraction step was added to the HPLC-MS/MS procedure. The HAAs monochloroacetic acid, dichloroacetic acid, and trichloroacetic acid were detected in the bulk sodium hypochlorite solutions by both the HPLC-MS/MS and the PCR-IC although the two methods disagree on reported concentrations.

Download or read book Liquid Chromatography Mass Spectrometric Analysis of Clinically and Pharmacologically Relevant Molecules written by Raghavi Kakarla and published by . This book was released on 2019 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography-Mass Spectrometry is an advanced analytical technique that offers high sensitivity and specificity and has been increasingly used for analysis of a wide variety of compounds including clinically and pharmacologically relevant molecules. In this dissertation we describe qualitative and quantitative liquid chromatographic mass spectrometric methods to analyze both small molecules and larger macromolecules that provide useful insights into diagnosis and management of several diseases. An LC-MS(/MS) analytical method includes extraction of analytes of interest from the matrix followed by liquid chromatographic separation and mass spectrometric detection. Chapter I describes pre-analytical workflows and sample pretreatment techniques and theories underlying LC-MS and instrumentation that are relevant to this work. The first chapter also describes the process of method development followed by validation guidelines for quantitative bio-analytical assays. Chapter II describes a novel, rapid, and simple quantitative mass spectrometric method for endogenous molecules in human bile that are associated with Cholangiocarcinoma and Cholelithiasis. The method was designed and validated to overcome problems suffered by conventional methods such as time-consuming extraction steps, carryover and unavailability of blank bile by employing simple dilution, flow injection and standard addition to matrix effects respectively. In Chapter III, a quantitative LC-MS/MS method was developed and validated for the determination of an antitumor drug in mouse brain to support an investigation to study the effectiveness of intracerebral microdialysis as an alternative route of administration. This method describes a two-step extraction process using Proteinase K and ethanol protein precipitation to overcome the low recovery and high matrix effects faced by previously reported methods. Chapter IV describes investigation of feasibility of employing a less commonly used proteolytic enzyme, aspartic acid N endopeptidase, in the digestion of prothrombin for qualitative LC-MS analysis. This study could be employed to study distribution of variants of des-gamma-carboxy-prothrombin, a biomarker which is elevated in hepatocellular carcinoma and vitamin K deficiency to further identify a more specific variant(s) as a biomarker. Finally, this dissertation is concluded with recommendations for qualitative and quantitative LC-MS research methodology based on the findings herein and future directions implicated by the impact of this work.

Download or read book Trace Elemental Speciation Using Chromatography capillary Electrophoresis Coupled to Inductively Coupled Plasma Mass Spectometry for Food Pharmaceutical and Environmental Analysis written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Trace element speciation has become an important issue in different fields of science. Research interests have moved from total element composition of the sample toward characterization of species that are responsible for biochemical and geochemical behavior of the elements. Information on elemental speciation in environmental and clinical material is vital in studies on possible mechanisms of element transport and/or degradation in the environment, element bioavailability and on possible metabolic pathways within living organisms. The current analytical approach for speciation analysis involves using hyphenation techniques, based on the combination of separation and detection steps into one while operating an on-line system. Both high performance liquid chromatography and capillary electrophoresis were the primary separation techniques utilized in this work. The inductively coupled plasma mass spectrometer was the technique of choice for element specific detection and as part of hyphenated systems for speciation analysis. The use of inductively coupled plasma mass spectrometry allows extremely low detection limits for the majority of the elements, has the advantage of linearity over a wide dynamic range, multi-elemental detection capability and the ability to perform isotopic analysis. The two most important features of an analytical tool suitable for speciation analysis are good selectivity and high sensitivity. However, the design of the analytical procedure is difficult, owing to the complex composition of the real-world sample, the diversity of physicochemical forms of the element, their lability and low concentrations. Sample preparation methods such as solid phase microextraction (SPME), solid phase extraction (SPE) and liquid-liquid extraction have been commonly used to overcome some of these difficulties. Thus improved sample collection, extraction, and purification techniques can help to overcome the problems associated with complex sample matrices. The above techniques were used separately or in combination for elemental speciation in food, pharmaceutical and environmental samples. These included the distribution of selenium and arsenic in various nuts, elemental association to different molecular weight fractions in Brazil Nuts, degradation products of levothyroxine in pharmaceutical tablets and iodophenols in river water samples.

Download or read book Metabolomics From Fundamentals to Clinical Applications written by Alessandra Sussulini and published by Springer. This book was released on 2017-01-28 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive view of metabolomics, from the basic concepts, through sample preparation and analytical methodologies, to data interpretation and applications in medicine. It is the first volume to cover metabolomics clinical applications while also emphasizing analytical and statistical features. Moreover, future trends and perspectives in clinical metabolomics are also presented. For researches already experienced in metabolomics, the book will be useful as an updated definitive reference. For beginners in the field and graduate students, the book will provide detailed information about concepts and experimental aspects in metabolomics, as well as examples and perspectives of applications of this strategy to clinical questions.

Download or read book NMR based Metabolomics written by Hector C Keun and published by Royal Society of Chemistry. This book was released on 2018-01-17 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the state of the art in the application of NMR spectroscopy to metabolomics and will be a key title for researchers and practitioners.

Download or read book Methodologies for Metabolomics written by Norbert W. Lutz and published by Cambridge University Press. This book was released on 2013-01-21 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.

Download or read book Processing Metabolomics and Proteomics Data with Open Software written by Robert Winkler and published by Royal Society of Chemistry. This book was released on 2020-03-19 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metabolomics and proteomics allow deep insights into the chemistry and physiology of biological systems. This book expounds open-source programs, platforms and programming tools for analysing metabolomics and proteomics mass spectrometry data. In contrast to commercial software, open-source software is created by the academic community, which facilitates the direct interaction between users and developers and accelerates the implementation of new concepts and ideas. The first section of the book covers the basics of mass spectrometry, experimental strategies, data operations, the open-source philosophy, metabolomics, proteomics and statistics/ data mining. In the second section, active programmers and users describe available software packages. Included tutorials, datasets and code examples can be used for training and for building custom workflows. Finally, every reader is invited to participate in the open science movement.

Download or read book Tandem Mass Spectrometry Molecular Characterization written by and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Ultra Performance Liquid Chromatography Mass Spectrometry written by Mu Naushad and published by CRC Press. This book was released on 2014-03-18 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a unique collection of up-to-date UPLC-MS/MS (ultra performance liquid chromatography-tandem mass spectrometric) methods for the separation and quantitative determination of pesticides, capsaicinoids, heterocyclic amines, aflatoxin, perfluorochemicals, acrylamide, procyanidins and alkaloids, lactose content, phenolic compounds, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.