Download or read book Purification Physical and Chemical Characterization of the Nicotinamide Adenine Dinucleotide specific Glutamate Dehydrogenase from Chlorella Sorokiniana written by Michael John Meredith and published by . This book was released on 1977 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Expression in the Cell Cycle written by G.M. Padilla and published by Elsevier. This book was released on 2012-12-02 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic Expression in the Cell Cycle provides an understanding of the molecular mechanisms that govern the expression of genetic information during the cell cycle. The initial five chapters describe the intimate relationships between the supramolecular complexes that form the basic structure of chromatin. Emphasis is placed on the dynamics of cycle-dependent changes in the structural organization of some of these components. Subsequent chapters demonstrate that small nuclear RNAs (SnRNA) are actively involved in gene regulation in eukaryotic cells; discuss the relationship between cell cycle regulation in the yeast Saccharomyces cerevisiae and transcription of ribosomal RNA genes; and describe the use of conditional lethal mutants to study the regulation of the cell cycle of eukaryotic cells. The remaining chapters discuss the concepts and methodologies employed to isolate and study specific cell cycle mutants of S. cerevisiae; the antiproliferative effect of interferon on cultured human fibroblasts; and the role of cell membrane and related subcellular elements in the control of proliferation, differentiation, and cell cycle kinetics.

Download or read book Purification and Partial Kinetic and Physical Characterization of Two NADP specific Glutamate Dehydrogenase Isoenzymes and Their Protein Precursors and Measurement of the Patterns of Accumulation and Rates of Degradation of Their Nonidentical Subunits in Synchronized Cells of Chlorella Cultured in Different Concentrations of Ammonia written by Newell F. Bascomb and published by . This book was released on 1985 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two ammonium-inducible, chloroplast-localized, NADP-specif ic glutamate dehydrogenases were purified from Chlorella sorokiniana . They were homopolymers of either alpha or beta subunits with molecular weights of 55,500 and 53,000, respectively. Purification was achieved by gel filtration (Sephacryl S-300), fast protein liquid chromatography (Pharmacia, Mono Q column), and substrate affinity chromatography (Pharmacia, NADP-Sepharose 4B, Type 4). These isoenzymes were separated by their differential binding to the substrate affinity column. Peptide mapping of purified alpha and beta subunits showed them to have a high degree of sequence homology. Because the ammonia K of alpha holoenzyme was sensitive to changes in NADPH concentration, its K ranged from 0.02 mM to 3.5 mM. With m changes in NADPH concentration, the aminonia K^ of beta holoenzyme remained constant at 70 mM. By use of SDS slab-gel electrophoresis and a Western blot/immunodetection procedure, patterns of accumulation of alpha and beta subunits (in their holoenzyme) were measured in cells cultured in media, containing different concentrations of ammonia. Pulse-chase experiments with Ssulfate were performed to measure the rates of degradation of the two isoenzymes. When the culture medium contained 2 mM ammonia or lower, cells accumulated only the alpha holoenzyme. Above 2 mM ammonia, cells contained both isoenzymes; however, their patterns of accumulation and rates of degradation were very different. The physiological role of alpha and beta holoenzymes appears to be ammonia assimilation at low and high external ammonia concentrations, respectively. From in vitro translation studies with total cellular poly(A)"^ RNA, isolated from cells engaged in synthesis of alpha or beta holoenzymes or both, it was concluded that alpha and beta subunits have protein precursor(s) of identical molecular weight (M = 58,500). When the putative protein-precursor (s) were incubated in vitro, with cell-free extracts from Chlorella cells, they were processed to proteins the size of alpha and beta subunits. From in vitro processing experiments, it was concluded that either two different precursors of the same molecular weight are specifically processed to give alpha and beta subunits, or the specificity or type of processing-enzyme changes to give the two subunits from a single precursor -protein.

Download or read book Directory of Graduate Research written by American Chemical Society. Committee on Professional Training and published by . This book was released on 1979 with total page 1030 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Dissertation Index Chemistry E O written by University Microfilms International and published by . This book was released on 1984 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Dissertation Index written by University Microfilms International and published by Ann Arbor, Mich. : University Microfilms International. This book was released on 1984 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book American Doctoral Dissertations written by and published by . This book was released on 1977 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Amino acid Peptide Protein Abstracts written by and published by . This book was released on 1979 with total page 1276 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Canadian Journal of Genetics and Cytology written by and published by . This book was released on 1983 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Dissertation Abstracts International written by and published by . This book was released on 1978 with total page 788 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1984 with total page 818 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1973- include the following subject areas: Biological sciences, Agriculture, Chemistry, Environmental sciences, Health sciences, Engineering, Mathematics and statistics, Earth sciences, Physics, Education, Psychology, Sociology, Anthropology, History, Law & political science, Business & economics, Geography & regional planning, Language & literature, Fine arts, Library & information science, Mass communications, Music, Philosophy and Religion.

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1978 with total page 848 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Dissertation Index Chemistry P Z written by and published by . This book was released on 1984 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of the Gene MRNAS Precursor Proteins and Mature Subunits Involved in the Synthesis of the NADP specific Glutamate Dehydrogenase Isoenzymes in Chlorella Sorokiniana written by Philip Miller and published by . This book was released on 1994 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book National Union Catalog written by and published by . This book was released on 1983 with total page 1032 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bibliography of Agriculture written by and published by . This book was released on 1982 with total page 2134 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Algal and Cyanobacterial Biotechnology written by R. C. Cresswell and published by Longman Scientific and Technical. This book was released on 1989 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: