Download or read book Pulsed Field Capillary Electophoresis of Nucleic Acids Using Variable Pulse Duration Protocols written by Michael James Navin and published by . This book was released on 1996 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Download or read book Pulsed Field Gel Electrophoresis written by Margit Burmeister and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents step-by-step protocols for users of Pulsed-Field Gel Electrophoresis (PFGE), whether novice or expert. This book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. It includes protocols designed to work even the first time they are used.
Download or read book Pulsed Field Gel Electrophoresis written by Bruce Birren and published by Academic Press. This book was released on 2012-12-02 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Reviews all types of pulsed field gel electrophoresis Describes all commercially available systems and summarizes advantages and limitations of each Includes step-by-step protocols for sample preparation and analysis Illustrated with photographs that depict How to run gels: What the results should look like What they look like when they go wrong Covers applications to a wide range of organisms Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism
Download or read book Pulsed field Capillary Electrophoresis of Nucleic Acids written by Yongseong Kim and published by . This book was released on 1996 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acid Electrophoresis written by Dietmar Tietz and published by Boom Koninklijke Uitgevers. This book was released on 1998-10-22 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.
Download or read book Capillary Electrophoresis of Nucleic Acids written by and published by Springer. This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Journal of Chromatography written by and published by . This book was released on 1997 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Handbook of Microbiology written by Emanuel Goldman and published by CRC Press. This book was released on 2015-06-04 with total page 1032 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Practical Handbook of Microbiology presents basic knowledge about working with microorganisms in a clear and concise form. It also provides in-depth information on important aspects of the field-from classical microbiology to genomics-in one easily accessible volume.This new edition retains the easy-to-use format of previous editions, with a lo
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1995 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Download or read book Glycoprotein Methods and Protocols written by Anthony P. Corfield and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Download or read book Molecular Identification Systematics and Population Structure of Prokaryotes written by Erko Stackebrandt and published by Springer Science & Business Media. This book was released on 2010-09-08 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.
Download or read book Molecular Bacteriology written by Neil Woodford and published by Springer Science & Business Media. This book was released on 1998-04-30 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1991 with total page 1484 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Journal of the Chinese Chemical Society written by Chinese Chemical Society and published by . This book was released on 1995 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rapid Detection Characterization and Enumeration of Foodborne Pathogens written by J. Hoorfar and published by American Society for Microbiology Press. This book was released on 2011-04-18 with total page 583 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detect foodborne pathogens early and minimize consumer exposure. • Presents the latest guidelines for fast, easy, cost-effective foodborne pathogen detection. • Enables readers to avoid common pitfalls and choose the most effective and efficient method, assemble the necessary resources, and implement the method seamlessly. • Includes first-hand laboratory experience from more than 85 experts from research centers across the globe.
