Download or read book Microbial Proteomics written by Dörte Becher and published by Humana. This book was released on 2019-10-11 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond. Chapter 18 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Microbial Proteomics written by Ian Humphery-Smith and published by Wiley-Interscience. This book was released on 2006-08-11 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed. Key topics covered include: Microbial pathogenesis at the proteome level Whole cell modeling Structural proteomics and computational analysis Biomolecular interactions Physiological proteomics Metabolic reconstruction using proteomics data While presenting the practical utility of proteomics data, the text is also clear on the field's current limitations, pointing to areas where further investigation is needed. Offering a state-of-the-art perspective from internationally recognized experts, this text is ideally suited for researchers and students across the gamut of genomic sciences, including biochemistry, microbiology, molecular biology, genetics, biomedical and pharmaceutical sciences, biotechnology, and veterinary science.

Download or read book MALDI TOF and Tandem MS for Clinical Microbiology written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2017-03-31 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry

Download or read book Applications in Forensic Proteomics written by Eric D. Merkley and published by . This book was released on 2020-10-09 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to forensic proteomics -- A proteomics tutorial -- Proteomic sample preparation techniques : toward forensic proteomic applications -- NextGen serology : leveraging mass spectrometry for protein-based human body fluid identification -- Informatics approaches to forensic body fluid identification by proteomic mass spectrometry -- Fingermarks as a new proteomic specimen : state of the art and perspective of in situ proteomics -- Human identification using genetically variant peptides in biological forensic evidence -- Proteomics in the analysis of forensic, archaeological, and paleontological bone -- Proteomics for microbial forensics -- ISO 17025 accreditation of method-based mass spectrometry for bioforensic analyses -- Unambiguous identification of ricin and abrin with advanced mass spectrometric assays -- Challenges in the development of reference materials for protein toxins -- The statistical defensibility of forensic proteomics.

Download or read book Recent Developments in Applied Microbiology and Biochemistry written by Viswanath Buddolla and published by Academic Press. This book was released on 2020-10-15 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a comprehensive treatment and understanding on application oriented microbial concepts, giving readers insights into recent developments in microbial biotechnology and medical, agricultural and environmental microbiology. - Discusses microbial proteome analyses and their importance in medical microbiology - Explores emerging trends in the prevention of current global health problems, such as cancer, obesity and immunity - Shows recent approaches in the production of novel enzymes from environmental samples by enrichment culture and metagenomics approaches - Guides readers through the status and recent developments in analytical methods for the detection of foodborne microorganisms

Download or read book Proteomics in Foods written by Fidel Toldrá and published by Springer Science & Business Media. This book was released on 2012-12-16 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food proteomics is one of the most dynamic and fast-developing areas in food science. The goal of this book is to be a reference guide on the principles and the current and future potential applications of proteomics in food science and technology. More specifically, the book will discuss recent developments and the expected trends of the near future in food proteomics. The book will be divided into two parts. The first part (7 chapters) will focus on the basic principles for proteomics, e.g., sample preparation, such as extraction and separation techniques, analytical instrumentation currently in use, and available databases for peptide and protein identification. The second part of the book (26 chapters) will focus on applications in foods. It will deal with quality issues related to post-mortem processes in animal foods and quality traits for all foods in general, as well as the identification of bioactive peptides and proteins, which are very important from the nutritional point of view. Furthermore, consumers are now extremely susceptible to food safety issues, and proteomics can provide reassurance with different safety aspects, such as food authenticity, detection of animal species in the food, and identification of food allergens. All of these issues will be covered in this book. It is also worth noting that both editors are internationally recognized experts in the field of food science, and both have edited numerous food science books and handbooks.

Download or read book Toxicity and Waste Management Using Bioremediation written by Rathoure, Ashok K. and published by IGI Global. This book was released on 2015-12-02 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioremediation is an emerging field of environmental research. The objective of a bioremediation process is to immobilize contaminants (reactants) or to transform them into chemical products that do not pose a risk to human health and the environment. Toxicity and Waste Management Using Bioremediation provides relevant theoretical and practical frameworks and the latest empircal research findings on the remediation of contaminated soil and groundwater using bioorganisms. Focusing on effective waste treatment methodologies and management strategies that lead to improved human and environmental health, this timely publication is ideal for use by environmenal scientists, biologists, policy makers, graduate students, and scholars in the fields of environmental science, chemistry, and biology.

Download or read book Phytomicrobiome Interactions and Sustainable Agriculture written by Amit Verma and published by John Wiley & Sons. This book was released on 2021-01-08 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to the role microbes play in the enhanced production and productivity of agriculture to feed our growing population Phytomicrobiome Interactions and Sustainable Agriculture offers an essential guide to the importance of ‘Phytomicrobiome’ and explores its various components. The authors – noted experts on the topic – explore the key benefits of plant development such as nutrient availability, amelioration of stress and defense to plant disease. Throughout the book, the authors introduce and classify the corresponding Phytomicrobiome components and then present a detailed discussion related to its effect on plant development: controlling factors of this biome, its behaviour under the prevailing climate change condition and beneficial effects. The book covers the newly emerging technical concept of Phytomicrobiome engineering, which is an advanced concept to sustain agricultural productivity in recent climatic scenario. The text is filled with comprehensive, cutting edge data, making it possible to access this ever-growing wealth of information. This important book: Offers a one-stop resource on phytomicrobiome concepts Provides a better understanding of the topic and how it can be employed for understanding plant development Contains a guide to sustaining agriculture using phytomicrobiome engineering Presents information that can lead to enhanced production and productivity to feed our growing population Written for students, researchers and policy makers of plant biology, Phytomicrobiome Interactions and Sustainable Agriculture offers a clear understanding of the importance of microbes in overall plant growth and development.

Download or read book Identification of Microorganisms by Mass Spectrometry written by Charles L. Wilkins and published by John Wiley & Sons. This book was released on 2005-12-16 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.

Download or read book Proteomics of Microorganisms written by Michael Hecker and published by Springer Science & Business Media. This book was released on 2003-07-18 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting with the discovery of penicillin, other antibiotics, and insulin, the quest for understanding and use of biological systems, i. e. , microorganisms and ani mal tissue, for the production of value products has lead to a dramatic increase in microbiological and bioengineering research in the last decades. Chemical and pharmaceutical companies quickly realized the huge commercial potential of these bioproducts and have spent millions of US dollars on R &D as well as on a build up of production facilities. Although there was limited knowledge about the cell's molecular mechanisms, which are the basis for the formation of the desired products, products from fermentation and extraction of biological matrices were a success right from the start. R&D projects within industry and academia on the continuous improvement of production processes, especially microbial productivity and down stream processing, allowed a fast return of investment and secured competitiveness in the market. Whereas the focus of such research projects was mainly on the discovery of strains with higher pro ductivity for the product of interest, e. g. , antibiotics, a lot of expertise and knowledge was generated allowing the use of biotechnological products and processes outside the pharmaceutical arena. The tremendous increase in knowl edge and the technological developments in microbial genetics where driven by these research projects and, accompanied with the advancements in nucleotide chemistry leading to a much better understanding of intracellular processes, served as a basis for modern molecular biology and recombinant biotech nology.

Download or read book The Social Biology of Microbial Communities written by Institute of Medicine and published by National Academies Press. This book was released on 2013-01-10 with total page 633 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with the germ theory of disease in the 19th century and extending through most of the 20th century, microbes were believed to live their lives as solitary, unicellular, disease-causing organisms . This perception stemmed from the focus of most investigators on organisms that could be grown in the laboratory as cellular monocultures, often dispersed in liquid, and under ambient conditions of temperature, lighting, and humidity. Most such inquiries were designed to identify microbial pathogens by satisfying Koch's postulates.3 This pathogen-centric approach to the study of microorganisms produced a metaphorical "war" against these microbial invaders waged with antibiotic therapies, while simultaneously obscuring the dynamic relationships that exist among and between host organisms and their associated microorganisms-only a tiny fraction of which act as pathogens. Despite their obvious importance, very little is actually known about the processes and factors that influence the assembly, function, and stability of microbial communities. Gaining this knowledge will require a seismic shift away from the study of individual microbes in isolation to inquiries into the nature of diverse and often complex microbial communities, the forces that shape them, and their relationships with other communities and organisms, including their multicellular hosts. On March 6 and 7, 2012, the Institute of Medicine's (IOM's) Forum on Microbial Threats hosted a public workshop to explore the emerging science of the "social biology" of microbial communities. Workshop presentations and discussions embraced a wide spectrum of topics, experimental systems, and theoretical perspectives representative of the current, multifaceted exploration of the microbial frontier. Participants discussed ecological, evolutionary, and genetic factors contributing to the assembly, function, and stability of microbial communities; how microbial communities adapt and respond to environmental stimuli; theoretical and experimental approaches to advance this nascent field; and potential applications of knowledge gained from the study of microbial communities for the improvement of human, animal, plant, and ecosystem health and toward a deeper understanding of microbial diversity and evolution. The Social Biology of Microbial Communities: Workshop Summary further explains the happenings of the workshop.

Download or read book Proteomics of Microbial Pathogens written by Peter R. Jungblut and published by John Wiley & Sons. This book was released on 2007-01-30 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-quality research articles on proteomic analyses of microbial pathogens, made available in a handy form. Containing proven, high-quality research articles selected from the popular PROTEOMICS journal, this is a current overview of the latest research into the proteomics analysis of microbial pathogens as well as several review articles.

Download or read book Environmental Microbiology and Biotechnology written by Anoop Singh and published by Springer Nature. This book was released on 2020-09-23 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides up-to-date information on the state of the art in applications of biotechnological and microbiological tools for protecting the environment. Written by leading international experts, it discusses potential applications of biotechnological and microbiological techniques in solid waste management, wastewater treatment, agriculture, energy and environmental health. This first volume of the book “Environmental Microbiology and Biotechnology,” covers three main topics: Solid waste management, Agriculture utilization and Water treatment technology, exploring the latest developments from around the globe regarding applications of biotechnology and microbiology for converting wastes into valuable products and at the same time reducing the environmental pollution resulting from disposal. Wherever possible it also includes real-world examples. Further, it offers advice on which procedures should be followed to achieve satisfactory results, and provides insights that will promote the transition to the sustainable utilization of various waste products.

Download or read book Lactic Acid Bacteria written by Wei Chen and published by Springer. This book was released on 2019-08-08 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the latest research and new techniques in the field of lactic acid bacteria, including comparative genomics, transcriptomics, proteomics and metabolomics. It also introduces the omics and functional evaluation in detail and shows the links between lactic acid bacteria and gut health and host immunity. Summarizing the biotechnological advances in lactic acid bacteria for food and health, it is a valuable resource for researchers and graduate students in the fields of food microbiology, bioengineering, food science, nutrition and health.

Download or read book Geomicrobiology Molecular and Environmental Perspective written by Alexander Loy and published by Springer Science & Business Media. This book was released on 2010-07-23 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The interaction of microorganisms with geological activities results in processes influencing development of the Earth’s geo- and biospheres. In assessing these microbial functions, scientists have explored short- and longterm geological changes attributed to microorganisms and developed new approaches to evaluate the physiology of microbes including microbial interaction with the geological environment. As the field of geomicrobiology developed, it has become highly interdisciplinary and this book provides a review of the recent developments in a cross section of topics including origin of life, microbial-mineral interactions and microbial processes functioning in marine as well as terrestrial environments. A major component of this book addresses molecular techniques to evaluate microbial evolution and assess relationships of microbes in complex, natural c- munities. Recent developments in so-called ‘omics’ technologies, including (meta) genomics and (meta)proteomics, and isotope labeling methods allow new insights into the function of microbial community members and their possible geological impact. While this book summarizes current knowledge in various areas, it also reveals unresolved questions that require future investigations. Information in these chapters enhances our fundamental knowledge of geomicrobiology that contributes to the exploitation of microbial functions in mineral and environmental biotechn- ogy applications. It is our hope that this book will stimulate interest in the general field of geomicrobiology and encourage others to explore microbial processes as applied to the Earth.

Download or read book Host Bacteria Interactions written by Annette C. Vergunst and published by Humana Press. This book was released on 2014-08-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Download or read book Difference Gel Electrophoresis DIGE written by Rainer Cramer and published by Humana Press. This book was released on 2012-02-07 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein analysis is increasingly becoming a cornerstone in deciphering the molecular mechanisms of life. Proteomics, the large-scale and high-sensitivity analysis of proteins, is already pivotal to the new life sciences such as Systems Biology and Systems Medicine. Proteomics, however, relies heavily on the past and future advances of protein purification and analysis methods. DIGE, being able to quantify proteins in their intact form, is one of a few methods that can facilitate this type of analysis and still provide the protein isoforms in an MS-compatible state for further identification and characterization with high analytical sensitivity. Differential Gel Electrophoresis: Methods and Protocols introduces the concept of DIGE and its advantages in quantitative protein analysis. It provides detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics. Divided into four concise sections, this detailed volume opens with the basics of DIGE, the technique and its practical details with a focus on the planning of a DIGE experiment and its data analysis. The next section introduces various DIGE methods from those employed by scientists world-wide to more novel methods, providing a glance at what is on the horizon in the DIGE world. The volume closes with an overview of the wide range of DIGE applications from Clinical Proteomics to Animal, Plant, and Microbial Proteomics applications. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Differential Gel Electrophoresis: Methods and Protocols can be used by novices with some background in biochemistry or molecular biology as well as by experts in Proteomics who would like to deepen their understanding of DIGE and its employment in many hyphenations and application areas. With its many protocols, applications, and methodological variants, it is also a unique reference for all who seek fundamental details on the working principle of DIGE and ideas for possible future uses of DIGE in novel analytical approaches.