Download or read book Proteome Analysis of Chloroplasts of Arabidopsis Thaliana written by Anders Jimmy Ytterberg and published by . This book was released on 2007 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteomic Analysis of Cold Stressed Arabidopsis Thaliana Chloroplasts written by Valentina Lucarotti and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Photosynthesis written by Eva-Mari Aro and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the expression of photosynthesis related genes including regulation both at transcriptional and translational levels. It reviews biogenesis, turnover, and senescence of thylakoid pigment protein complexes and highlights some crucial regulatory steps in carbon metabolism.
Download or read book Chloroplast Research in Arabidopsis written by R Paul Jarvis and published by Humana Press. This book was released on 2011-08-31 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.
Download or read book Plant Proteomics written by Jesus V Jorrin-Novo and published by Humana. This book was released on 2021-06-11 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Structural Aspects Of Protein Synthesis 2nd Edition written by Anders Liljas and published by World Scientific. This book was released on 2013-06-06 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly illustrated book provides an up-to-date description of the structure and function of the translation system including ribosomes, tRNAs, translation factors, antibiotics and aminoacyl-tRNA synthetases.Research on translation is undergoing rapid changes and is receiving significant attention as evidenced by the Nobel Prize in Chemistry 2009. The structural research by crystallography and cryo-EM forms part of an interactive framework that involves biochemistry and molecular computation. The book provides a comprehensive overview of translation in light of the structural results. It is a valuable resource for scientists in this and related fields, as well as for students taking courses with a focus on translation.There is no other book in this field currently except the previous edition of this book. The authors have for a long time worked in the field of structure and function of the translation system.
Download or read book Stress induced Effects on the Chloroplast Proteome of Arabidopsis Thaliana written by Jeannette Kley and published by . This book was released on 2011 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Proteomics written by Hervé Thiellement and published by Humana. This book was released on 2006-09-22 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).
Download or read book Analysis of the Arabidopsis Thaliana Chloroplast Outer Envelope Membrane Subproteome written by Alice Berg and published by . This book was released on 2009 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Terminal Profiling written by Oliver Schilling and published by Humana. This book was released on 2017-03-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. “br>Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.
Download or read book Omics Analysis of High Energy Arabidopsis Thaliana written by Chao Liang and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Omics Analysis of High-energy Arabidopsis Thaliana" by Chao, Liang, 梁超, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Arabidopsis thaliana purple acid phosphatase 2 (AtPAP2) is a phosphatase dually targeted to both chloroplasts and mitochondria. Overexpression (OE) of AtPAP2 in Arabidopsis thaliana was reported to speed up plant growth and promote flowering, seed yield and biomass at maturity in a previous study. Under long-day (16 hours light at 22C / 8 hours dark at 18C) growth conditions, the leaves of 20-day-old OE lines contained significant higher sucrose and glucose than the wild-type (WT) plants, reflecting their high energy status. The AtPAP2 OE line is thus a good model to investigate the impact of high energy on the global physiological changes in Arabidopsis. In this study, the systems biology of the high-energy plants, in terms of transcriptome, proteome, metabolome and small RNA (sRNA) expression profiles were examined. Liquid chromatography mass spectrophotometry (LC-MS/MS) and enzyme assays were both employed to measure the content of ADP/ATP/NAD/NADP/NADPH in the leaves of 20-day-old OE and WT lines at 3 time points (t = 0, end of night; t = 1, 1 h after light was on; t = 8, 8 h after light was on). My results showed that the ATP and ADP contents were significantly increased in the OE line at all the three time points. In the dark, since mitochondria is the major source of ATP supply in plant cells, the comparison of omics data between the OE and WT lines at t = 0 reflected the impact of high ATP output from mitochondria on plant leaves. Similarly, the comparison between the OE and WT lines at t = 8 reflected the impact of higher ATP output from chloroplast of plant leaves. By RNA sequencing (RNA-Seq) technology, transcriptome profiles between OE and WT at all the three time points (t = 0, 1, and 8 h after illumination) were compared. The transcripts of 29,435 genes were detected in all six datasets. Moreover, transcripts encoded by the chloroplast and mitochondrial genomes, which were not reported in the previous microarray studies were also sequenced. Genes of the central energy conversion pathways that were differentially transcribed between the OE and WT lines were identified. In proteomics studies, 2663 proteins were identified by the isobaric tags for relative and absolute quantification (iTRAQ) labeled technique. The proteins with significantly altered in protein abundances (P Small RNA studies showed that the expression of miR173, which initiates the generation of tasiRNAs from TAS1 and TAS2 loci, was significantly increased in the leaves of OE lines. These tasiRNAs target the mRNAs of various pentatricopeptide repeat (PPR) and tetratricopeptide repeat (TPR) proteins, which control RNA metabolism in chloroplasts and mitochondria. A network of miR173-tasiRNAs-PPR/TPR was established to present the changes in the OE lines. In summary, my study systematically investigated the global impact of high energy status to the metabolome, transcriptome, proteome and sRNAs profiles of Arabidopsis. Two diagrams were presented to illustrate the impacts of high energy status on the physiology of leaf cells under light and dark conditions, respectively.
Download or read book The Chloroplast Lumen Proteome of Arabidopsis Thaliana written by Maria Schubert and published by . This book was released on 2006 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Chloroplast written by Anna Stina Sandelius and published by Springer Science & Business Media. This book was released on 2008-12-11 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chloroplasts are vital for life as we know it. At the leaf cell level, it is common knowledge that a chloroplast interacts with its surroundings – but this knowledge is often limited to the benefits of oxygenic photosynthesis and that chloroplasts provide reduced carbon, nitrogen and sulphur. This book presents the intricate interplay between chloroplasts and their immediate and more distant environments. The topic is explored in chapters covering aspects of evolution, the chloroplast/cytoplasm barrier, transport, division, motility and bidirectional signalling. Taken together, the contributed chapters provide an exciting insight into the complexity of how chloroplast functions are related to cellular and plant-level functions. The recent rapid advances in the presented research areas, largely made possible by the development of molecular techniques and genetic screens of an increasing number of plant model systems, make this interaction a topical issue.
Download or read book Chloroplast Research in Arabidopsis written by R. Paul Jarvis and published by Humana Press. This book was released on 2016-08-23 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.
Download or read book Chloroplast Biogenesis written by Udaya C. Biswal and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chloroplast is the organelle where the life-giving process photosynthesis takes place; it is the site where plants and algae produce food and oxygen that sustain our life. The story of how it originates from proplastids, and how it ultimately dies is beautifully portrayed by three authorities in the field: Basanti Biswal, Udaya Biswal and M. K. Raval. I consider it a great privilege and honor to have been asked to write this foreword. The book ' Chloroplast biogenesis: from proplastid to gerontoplast' goes much beyond photosynthesis. The character of the book is different from that of many currently available books because it provides an integrated approach to cover the entire life span of the organelle including its senescence and death. The books available are mostly confined to the topics relating to the 'build up' or development of chloroplast during greening. The story of organelle biogenesis without description of the events associated with its regulated dismantling during genetically programmed senescence is incomplete. A large volume of literature is available in this area of chloroplast senescence accumulated during the last 20 years. Although some of the findings in this field have been organized in the form of reviews, the data in the book are generalized and integrated with simple text and graphics. This book describes the structural features of prop las tid and its transformation to fully mature chloroplast, which is subsequently transformed into gerontoplast exhibiting senescence syndrome. The book consists of five major chapters.
Download or read book Plant Membrane Proteomics written by Hans-Peter Mock and published by Humana. This book was released on 2017-10-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.
Download or read book Probing the Arabidopsis Thaliana Chloroplast Outer Envelope Membrane Proteome and Interactome written by Emily Shortt and published by . This book was released on 2013 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt:
