Download or read book Protein Engineering for Therapeutics Scaffolds written by and published by . This book was released on 2012 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authorsThis volume is split into sections that cover subjects such as peptides, and scaffolds.

Download or read book Protein Engineering for Therapeutics written by K. Dane Wittrup and published by Academic Press. This book was released on 2012 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike. Iinternational board of authors. This volume is split into sections that cover subjects such as Peptides, and Scaffolds

Download or read book Protein Engineering for Therapeutics Part A written by K. Dane Wittrup and published by Academic Press. This book was released on 2012-01-11 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Download or read book Protein Engineering for Therapeutics Part B written by and published by Academic Press. This book was released on 2012-02-02 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as peptides, and scaffolds

Download or read book Protein Engineering for Therapeutics Part A written by and published by Academic Press. This book was released on 2012-01-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Download or read book Protein Engineering for Therapeutics Part B written by and published by Academic Press. This book was released on 2012-01-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds

Download or read book Protein Engineering for Therapeutics Part B written by and published by Academic Press. This book was released on 2012-01-25 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds - Chapters provide an invaluable resource for academics, researchers and students alike - Iinternational board of authors - This volume is split into sections that cover subjects such as peptides, and scaffolds

Download or read book Protein Therapeutics written by Tristan Vaughan and published by John Wiley & Sons. This book was released on 2017-07-28 with total page 754 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practice-oriented two volume handbook, professionals from some of the largest biopharmaceutical companies and top academic researchers address the key concepts and challenges in the development of protein pharmaceuticals for medicinal chemists and drug developers of all trades. Following an introduction tracing the rapid development of the protein therapeutics market over the last decade, all currently used therapeutic protein scaffolds are surveyed, from human and non-human antibodies to antibody mimetics, bispecific antibodies and antibody-drug conjugates. This ready reference then goes on to review other key aspects such as pharmacokinetics, safety and immunogenicity, manufacture, formulation and delivery. The handbook then takes a look at current key clinical applications for protein therapeutics, from respiratory and inflammation to oncology and immune-oncology, infectious diseases and rescue therapy. Finally, several exciting prospects for the future of protein therapeutics are highlighted and discussed.

Download or read book Protein Engineering for Therapeutics written by and published by Academic Press. This book was released on 2012-01-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Download or read book Engineering Bivalency and Bispecificity in Protein Therapeutics and Protein Scaffold Development written by Cassie J. Liu and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Multivalent and multifunctional proteins are abundant in nature. The avidity effect, a core principle of multivalent molecules, allows these proteins to tune biological functions and responses across a large dynamic range with varying specificity. Many protein therapeutics also take advantage of this evolvable tunability, the canonical example being the antibody. This thesis consists of two separate sections. Section One: I focus on the avidity effect of multivalent proteins and its therapeutic uses. As an illustrative case, our lab had previously created a protein variant that monomerically binds the c-MET tyrosine kinase. By dimerizing this variant, we find that we greatly increase its efficacy through increased apparent affinity to cell surface c-MET. Section Two: I focus on multispecific proteins and their uses in medical applications. Most current therapeutic protein scaffolds are single protein domains with monomeric binding and function. Combining these scaffolds to create multispecific functionality presents manufacturing challenges. Here the vascular endothelial growth factor (VEGF) protein is used as a stable, naturally bivalent scaffold for engineering bispecific proteins.

Download or read book Engineering Non antibody Scaffolds and Growth Factor Ligands as Protein Therapeutics and Diagnostics written by Cheuk Lun Leung and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein therapeutics have emerged as an important class of pharmaceuticals, with monoclonal antibodies making up the largest group of protein drugs. While antibodies have many desirable characteristics, there are some applications in which they may be the best option. In this dissertation, we describe alternative classes of proteins that can complement the use of antibodies in the development of protein therapeutics and diagnostics. Cystine knots, or knottins, have highly desirable properties for use as molecular imaging agents due to their small size and stable structure. Previous approaches to engineering knottins have focused on a small subset of knottin scaffolds; in this work we extend those studies to engineering Agatoxin, a knottin from spider venom, for high affinity binding to tumor associated integrins. Growth factor ligand-receptor interactions modulate many cell signaling pathways and biological processes. The vascular endothelial growth factor (VEGF) and the platelet-derived growth factor (PDGF) mediate two important signaling pathways involved in angiogenesis. In this work, we used the structural similarity of PDGF and VEGF to rationally design a chimeric PDGF/VEGF ligand, creating a multi-specific antagonist of their receptors PDGFR and VEGFR. The PDGF/VEGF chimera was then engineered using combinatorial methods, including yeast surface display, for increased binding affinity to PDGFR and VEGFR. The resulting engineered protein variant strongly inhibited PDGFR and VEGFR signaling with in vitro cell models. Given the important roles of PDGFR and VEGFR in angiogenesis, the PDGF/VEGF chimera has potential application in anti-angiogenesis therapy. Collectively, the engineered proteins in this work demonstrate the promise of using non-antibody scaffolds and growth factor ligands for the development of protein therapeutics and diagnostics.

Download or read book Peptide And Protein Engineering For Biotechnological And Therapeutic Applications written by Pierre Rousselot-pailley and published by World Scientific. This book was released on 2023-01-06 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and proteins are crucial biomolecules in life. The manifold functions they carry out range from molecular recognition and signaling to catalysis and immune response. However, the native systems are limited to a reduced toolbox of chemical functionalities as well as tridimensional structures. Widening these toolboxes could pave the way to engineer peptides and proteins with enhanced properties compared to their native counterparts and/or with structures and functions unprecedented in Nature. Advances in the chemical and biological synthesis of peptides and proteins, in computational tools, in molecular biology and in high-throughput screening methods are making this realm possible.This book aims to give an overview of the last developments in the field of peptide and protein engineering. It comprises a collection of chapters that span from the production of simple non-proteinogenic building blocks and peptidic scaffolds of different sizes and structures to more complex systems including peptide-based nanomaterials, enzymes and artificial metalloenzymes. Different strategies are described where chemical and biological tools have been developed and combined to attain the desired properties and sought functionalities.The diverse systems described in this book highlight the progress in this important field and represent the starting points for the development of functional biomolecules, biomaterials and hybrid systems capable of addressing key societal challenges of our times in relevant areas such health, environment and energy.

Download or read book Protein Engineering for Therapeutics Part B written by and published by Academic Press. This book was released on 2012-02-02 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Peptides, and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as peptides, and scaffolds

Download or read book Protein Engineering written by D. Srinivasa Rao and published by LAP Lambert Academic Publishing. This book was released on 2012-08 with total page 120 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering is the generation of novel protein molecule, endowed with new function by mutagenesis and completely novel molecules, it is a one of the most promising developments in engineering to understand and control a protein's function. At present proteins fold remains elusive, theoretical and experimental studies of protein folding have come a long way. Protein libraries can enable to generate novel molecular tools and potential therapeutics by directed evolution and engineered proteins are important as therapeutics, diagnostics, and imaging agents in biological systems. Combining selection/directed evolution with scaffold-based protein libraries provides an excellent route to engineer new protein function.

Download or read book Protein Engineering and Design written by Sheldon J. Park and published by CRC Press. This book was released on 2009-09-25 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science

Download or read book Protein Engineering written by Mallorie N. Sheehan and published by Nova Science Publishers. This book was released on 2013-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.

Download or read book Phage Display written by Carlos F. Barbas and published by CSHL Press. This book was released on 2001 with total page 724 pages. Available in PDF, EPUB and Kindle. Book excerpt: Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.