Download or read book Protein Adsorption to Surfaces with Varying Characteristics written by Yeong-Shang Lin and published by . This book was released on 1993 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Adsorption to Vial Surfaces Quantification Structural and Mechanistic Studies written by Johannes Mathes and published by Cuvillier Verlag. This book was released on 2010-09-21 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The adsorption of therapeutic proteins to different surfaces in the course of fill and finish, storage, or administration is critical and has shown to cause severe problems which should not be underestimated. Among them is the loss of content but also, potentially, protein structural changes, accompanied by the risk of therapy failure or immunological reactions. In this context, special attention has to be paid to primary packaging containers, to which the protein has an enduring surface contact. Against this background, the adsorption of a modern therapeutic protein, i.e. an IgG1 antibody, to vials was investigated within the scope of this thesis. The first priority had borosilicate glass, followed by siliconized borosilicate glass and polyolefin plastic. The use of model surfaces was almost completely avoided. The studies comprise the quantification of surface-bound protein subject to variable formulation compositions and surface qualities as well as a detailed characterization of the adsorbed protein layer including protein structural alterations caused by the adsorption process. In the presented investigations, the main focus was on the equilibrium state of adsorption at conditions mainly within the scope of pharmaceutical relevance, but also beyond, if necessary, for the acquisition of fundamental knowledge on the adsorption mechanism.

Download or read book Biological Interactions on Materials Surfaces written by David A. Puleo and published by Springer Science & Business Media. This book was released on 2009-06-26 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success or failure of biomaterials, whether tissue engineered constructs, joint and dental implants, vascular grafts, or heart valves, depends on molecular-level events that determine subsequent responses of cells and tissues. This book presents the latest developments and state-of-the-art knowledge regarding protein, cell, and tissue interactions with both conventional and nanophase materials. Insight into these biomaterial surface interactions will play a critical role in further developments in fields such as tissue engineering, regenerative medicine, and biocompatibility of implanted materials and devices. With chapters written by leaders in their respective fields, this compendium will be the authoritative source of information for scientists, engineers, and medical researchers seeking not only to understand but also to control tissue-biomaterial interactions.

Download or read book Proteins at Interfaces II written by and published by . This book was released on 1995 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair written by Mario Barbosa and published by Woodhead Publishing. This book was released on 2017-09-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair highlights the various important considerations that go into biomaterial development, both in terms of fundamentals and applications. After covering a general introduction to protein and cell interactions with biomaterials, the book discusses proteins in biomaterials that mimic the extracellular matrix (ECM). The properties, fabrication and application of peptide biomaterials and protein-based biomaterials are discussed in addition to in vivo and in vitro studies. This book is a valuable resource for researchers, scientists and advanced students interested in biomaterials science, chemistry, molecular biology and nanotechnology. - Presents an all-inclusive and authoritative coverage of the important role which protein and peptides play as biomaterials for tissue regeneration - Explores protein and peptides from the fundamentals, to processing and applications - Written by an international group of leading biomaterials researchers

Download or read book Prediction of Protein Adsorption on Engineered Surfaces for Biomedical and Industrial Applications written by Maria Eugenia Arias Montecillo and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "This thesis describes the advancement of the Bio-molecular Adsorption Database (BAD) to more accurately predict protein adsorption. To advance the model of the BAD, data was first: collected from published literature in the last ten years, processed to be added to BAD, and used in calculations to advance the BAD ability to accurately predict protein adsorption.Protein adsorption at solid-liquid interfaces is crucial in many biomedical and industrial applications. These applications include biomaterials, protein microarrays, lab-on-a-chip devices and bioreactors. (Vasina et al., 2009)In the biomaterial area, the first response after contact of an implant material with a biological fluid, is the adsorption of proteins on to the surface. The adsorbed protein layer plays a role in the regulation of the biological response to the material and therefore bio-compatibility. It is from the adsorbed protein layer that the cells in the system obtain information about the foreign surface determining the cell behaviour. This cell behaviour can range from: adhesion establishment and proliferation of cells in the proximity to the material or the rejection and inflammation at the interface due to over layering and denaturation. (Wilson, Clegg, Leavesley, & Pearcy, 2005)In the protein microarrays and micro/nano- devices area, protein adsorption is of interest because of the need finding an optimum balance to maximized sensitivity and/or activity for analytical functions. This is done by preserving the proteins native characteristics and minimizing protein denaturation. (Vasina et al., 2009)In the bioreactors area, protein adsorption plays a role in membrane fouling, leading to increased pressures of operation and frequent biofouling clear out. As such, it is important to choose materials and conditions to minimize protein adsorption and biofouling in bioreactors. (Ognier, Wisniewski, & Grasmick, Influence of macromolecule adsorption during filtration of a membrane bioreactor mixed liquor suspension, 2002)Regardless of the considerable attention and research in the last 50 years, protein adsorption prediction had not been attained with sufficient accuracy for engineering applications. The original work into BAD, addressed protein adsorption prediction by archiving protein adsorption data and applying statistical analysis to the data. (Vasina et al., 2009)This approach correlated accurately the input variables to protein adsorption. Examples include: protein concentration in solution, protein characteristics, fluid environment conditions and surface parameters to the output variable or protein concentration on the surface. (Vasina et al., 2009)" --

Download or read book Hydrogels in Medicine and Pharmacy written by Nikolaos A. Peppas and published by CRC Press. This book was released on 2019-08-15 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: First Published in 1986, this book offers a full, comprehensive guide to the application of hydrogels in medicine. Carefully compiled and filled with a vast repertoire of notes, diagrams, and references this book serves as a useful reference for students of medicine and other practitioners in their respective fields.

Download or read book Biomaterials Surface Science written by Andreas Taubert and published by John Wiley & Sons. This book was released on 2013-07-23 with total page 599 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the interface of biology, chemistry, and materials science, this book provides an overview of this vibrant research field, treating the seemingly distinct disciplines in a unified way by adopting the common viewpoint of surface science. The editors, themselves prolific researchers, have assembled here a team of top-notch international scientists who read like a "who's who" of biomaterials science and engineering. They cover topics ranging from micro- and nanostructuring for imparting functionality in a top-down manner to the bottom-up fabrication of gradient surfaces by self-assembly, from interfaces between biomaterials and living matter to smart, stimuli-responsive surfaces, and from cell and surface mechanics to the elucidation of cell-chip interactions in biomedical devices. As a result, the book explains the complex interplay of cell behavior and the physics and materials science of artificial devices. Of equal interest to young, ambitious scientists as well as to experienced researchers.

Download or read book Adsorption and Desorption of Proteins from Environmentally and Medically relevant Surfaces written by Hanh Thi My Phan and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein function can be influenced by attachment to surfaces. Understanding of protein adsorption behavior responsible for these functional changes is important for various environmental applications, including prion disease transmission. Infectious prions, a causative agent of prion disease, strongly bind to soil surfaces and medical tools and remain infectious. Studies of prion attachment to soils in the environment and to medical surfaces from the various biologic excreta in which prions are present is limited. To contribute to the knowledge of prion adsorption to surfaces, investigations of different proteins including bovine serum albumin (BSA) and alpha-synuclein adsorption to a variety of environmentally- and medically- relevant surfaces was conducted using quartz crystal microbalance with dissipation and spectroscopic ellipsometry. Adsorption and desorption of BSA, a model protein, varied as a function of surface properties, BSA concentrations, and pH. The initial adsorption rates, adsorbed layer thickness, and molecule arrangements of BSA adsorbed to the various charged surfaces were strongly dependent on the functional tail groups of the surfaces. alpha-Synuclein protein, a surrogate for the prion protein, shares many pathologically common traits with prion protein. Understanding the behavior of alpha-synuclein after binding to environmental surfaces can provide crucial insights into prion conformation and orientation on environmentally- and medically-relevant surfaces. Increased alpha-synuclein adsorption was observed on the environmental surface compared to the medical surface. alpha-Synuclein can bind to multiple surface types and decontamination was surface-dependent. Competitive matrices were also found to influence prion adsorption to soil surfaces. Prions were not likely to adsorb to soil in saliva while increased adsorption of prions were observed in the presence of urine or brain homogenate when compared to a buffer solution. Atomic force microscopy images of prion conformation adsorbed to mica, stainless steel, and SiO 2 surfaces were reported. These data provide evidence of the influences of the competitive matrices on prion adsorption to soil surfaces and fundamental information on prion conformational change upon adsorption to environmentally- and medically-relevant surfaces.

Download or read book Nucleic Acids and Proteins in Soil written by Paolo Nannipieri and published by Springer Science & Business Media. This book was released on 2006-09-22 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.

Download or read book Mechanical Engineering in Biomedical Application written by Jay Prakash Srivastava and published by John Wiley & Sons. This book was released on 2024-01-02 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: MECHANICAL ENGINEERING IN BIOMEDICAL APPLICATIONS The book explores the latest research and developments related to the interdisciplinary field of biomedical and mechanical engineering offering insights and perspectives on the research, key technologies, and mechanical engineering techniques used in biomedical applications. The book is divided into several sections that cover different aspects of mechanical engineering in biomedical research. The first section focuses on the role of additive manufacturing technologies, rehabilitation in healthcare applications, and artificial recreation of human organs. The section also covers the advances, risks, and challenges of bio 3D printing. The second section presents insight into biomaterials, including their properties, applications, and fabrication techniques. The section also covers the use of powder metallurgy methodology and techniques of biopolymer and bio-ceramic coatings on prosthetic implants. The third section covers biofluid mechanics, including the mechanics of fluid flow within our body, the mechanical aspects of human synovial fluids, and the design of medical devices for fluid flow applications. The section also covers the use of computational modeling to study the blockage of carotid arteries. The final section elaborates on soft robotic manipulation for use in medical sciences. Audience The book provides practical insights and applications for mechanical engineers, biomedical engineers, medical professionals, and researchers working on the design and development of biomedical devices and implants.

Download or read book Adsorption and Bacterial Adhesion Characteristics of Proteins Microbial Growth Media and Milk on Abiotic Surfaces Under Static and Laminar Flow Conditions written by Nikhil Hiremath and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Understanding the adhesion of bio-molecules and microorganisms to abiotic surfaces is an important consideration in food industry. The formation of these biofilms can have deleterious effects leading to contamination of food or materials coming into contact with them, causing spoilage &/or safety concerns. Among the biomolecules proteins are known to adhere to the surface very rapidly, followed by other biomolecules and microorganisms. In this study the two abiotic model surfaces, were conditioned with proteins [bovine serum albumin, sodium caseinate], microbial growth media [tryptic soya broth] and milk [whole milk, skim milk] under static and dynamic flow processing conditions. The extent of protein adsorbed to surfaces was quantified using the micro BCA protein assay. The food contact surfaces conditioned or without conditioning, were characterized using contact angle measurements to obtain various surfaces thermodynamic properties. The conditioning solutions were also characterized by dynamic light scattering and zeta potential measurements. The total free energy of interaction was calculated using the thermodynamic and the extended XDLVO models, and the adhesive potential of the biofilms was evaluated. The effect of preconditioning on the adhesion of bacteria to mica and polystyrene were studied. In addition the distribution of conditioning solutions and bacteria on the surface was evaluated using atomic force microscopy. The results under static conditions showed that the amount of proteins adsorbed on mica was much higher than polystyrene surfaces which may be because mica is more hydrophilic and polystyrene more hydrophobic. Sodium caseinate had the highest adsorption on both mica and polystyrene when compared to other conditioning solutions. The thermodynamic properties of conditioned surfaces showed that they favored electron-donating or Lewis base properties which are typical for proteins in hydrated states. DLS and Zeta potential results indicated that proteins were in aggregated states in solutions and had a negative surface charge. Preconditioning of mica and polystyrene surfaces altered their surface thermodynamic properties, especially, the electron donor and acceptor components of the surface free energy. This had a significant impact on the resulting hydrophobicity of the preconditioned surfaces as compared to the surfaces which were not conditioned. The computed free energy of interaction using the two models indicated the adhesion potential of conditioning solutions (in most cases) on both surfaces, but failed to accurately predict the extent of protein adsorption on surfaces. Under laminar flow conditions the amount of protein adsorbed onto mica and polystyrene surface was approximately two times higher than those obtained under static conditions. The DLS measurements of conditioning solutions showed that particle sizes of the conditioning solutions were more polydisperse than under static conditions. Dynamic flow condition also altered the surface thermodynamic properties of mica and polystyrene as observed with static conditions. The AFM analysis of both static and flow conditioned surfaces revealed that the conditioning film formed was not a continuous film but a heterogeneous deposit and attached in the form of aggregates. The adhesion of Listeria innocua to mica and polystyrene surfaces that were preconditioned with BSA, TSB and CAS under laminar flow showed lower adhesion values in comparison with those under static settings. However, with whole milk and skim milk, higher adhesion values of L. innocua were observed under flow conditions as compared with static conditions indicating bio-film source dependent variations. It was also observed that L. innocua cell adhesion on mica and polystyrene surfaces preconditioned with CAS WML and SML under static conditions were lower than Listeria monocytogenes under similar conditions, indicating strain dependent variations." --

Download or read book Proteins at Solid Liquid Interfaces written by Philippe Déjardin and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.

Download or read book Adsorption and the Gibbs Surface Excess written by D. Chattoraj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 478 pages. Available in PDF, EPUB and Kindle. Book excerpt: The subject matter of this book is not entirely new in that much has been written about the state of the interfacial chemistry in other textbooks. However, the authors have found that there is really a need for a book which can lead an investigator, a student, or a beginn er through the in-depth understanding and descriptions involved in the derivations and usage of the adsorption equations based on the unified concept of the Gibbs surface excess. The derivation of the original Gibbs equation for the liquid interface has been amplified with reformulations and criticisms. Attempts have been frequently made to relate the surface excess quantities with the thermodynamic properties of the physically defined interfacial phase. In the last two decades, useful applications of the Gibbs equations have been made for the study of adsorption at solid-liquid as weIl as liquid-gas interfaces formed in the presence of the multicomponent solutions in the bulk. These recent treatments become useful for the experimental studies of the adsorption of electrolytes and interfacial properties related to neutral and charged monolayers. The consistency of the experimental results with those to be expected from the theories of the electrical double layer needs careful examination. An attempt has been made to present in a single volume all these developments based on the unified concept of the Gibbs surface excess. The study of the adsorption from solution is of considerable importance in the field of biology and in many other branches of applied science.

Download or read book Biomaterials Science written by Buddy D. Ratner and published by Elsevier. This book was released on 2004-08-18 with total page 879 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this bestselling title provides the most up-to-date comprehensive review of all aspects of biomaterials science by providing a balanced, insightful approach to learning biomaterials. This reference integrates a historical perspective of materials engineering principles with biological interactions of biomaterials. Also provided within are regulatory and ethical issues in addition to future directions of the field, and a state-of-the-art update of medical and biotechnological applications. All aspects of biomaterials science are thoroughly addressed, from tissue engineering to cochlear prostheses and drug delivery systems. Over 80 contributors from academia, government and industry detail the principles of cell biology, immunology, and pathology. Focus within pertains to the clinical uses of biomaterials as components in implants, devices, and artificial organs. This reference also touches upon their uses in biotechnology as well as the characterization of the physical, chemical, biochemical and surface properties of these materials. - Provides comprehensive coverage of principles and applications of all classes of biomaterials - Integrates concepts of biomaterials science and biological interactions with clinical science and societal issues including law, regulation, and ethics - Discusses successes and failures of biomaterials applications in clinical medicine and the future directions of the field - Cover the broad spectrum of biomaterial compositions including polymers, metals, ceramics, glasses, carbons, natural materials, and composites - Endorsed by the Society for Biomaterials

Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.Key Features* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:* detailed data on biological stability under various bioseparation conditions* extensive case studies from the literature on separation processes, validation, and economics* simplified analysis of protein refolding and inactivation mechanisms* consideration of adsorption theories and the effect of heterogeneity* coverage of both classical and novel bioseparation techniques, including chromatographic procedures

Download or read book Polymeric Biomaterials Revised and Expanded written by Severian Dumitriu and published by CRC Press. This book was released on 2001-11-29 with total page 1192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offering nearly 7000 references-3900 more than the first edition-Polymeric Biomaterials, Second Edition is an up-to-the-minute source for plastics and biomedical engineers, polymer scientists, biochemists, molecular biologists, macromolecular chemists, pharmacists, cardiovascular and plastic surgeons, and graduate and medical students in these disciplines. Completely revised and updated, it includes coverage of genetic engineering, synthesis of biodegradable polymers, hydrogels, and mucoadhesive polymers, as well as polymers for dermacosmetic treatments, burn and wound dressings, orthopedic surgery, artificial joints, vascular prostheses, and in blood contacting systems.