Download or read book Production and optimization of extracellular lipase by Bacillus subtilis using submerged fermentation written by Prem Jose Vazhacharickal and published by Prem Jose. This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipase is an extracellular enzyme in food industry and is produced by different microbial and fungal sources by submerged and solid state fermentation process. In the present study, Bacillus subtilis was employed for the production of lipase in peptone broth with olive oil as inducer. Maximum lipase production was observed at 96 hours of incubation and NH4Cl (40.75 U/ml) as salt and peptone (24U/ml) as nitrogen source and olive oil (24 U/ml) as inducer. The results of the present study demonstrate that Bacillus subtilis is ideal for extracellular production at industrial level.

Download or read book Optimization of Extracellular Lipase Production by Thermotolerant Bacteria Isolated from a Hot Spring written by Alli Malar A/P Harikrishnan and published by . This book was released on 2017 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is high demand for lipase globally where more than 1000 tons of it produced each year. A lipase produced from thermophilic bacteria is revised to have the advantage of thermal stability at 65°C-70°C, which makes them good candidates for lipid modifications which is most effective at high temperature. Thus, main focus of this research is to find the best lipase producing thermotolerant bacteria on its optimum condition of fermentation. The first step on finding lipase producer is to screen the isolated thermotolerant bacterial strains on tributyrin agar plate to check for zone of hydrolysis. Then, followed by screening under submerged fermentation. Next, lipase assay was carried out to find out the best lipase producer among the strains and finally the optimum condition for producing lipase investigated.

Download or read book Extracellular Lipase Production from Bacillus Sp Partial Purification written by Haniya Mazhar and published by LAP Lambert Academic Publishing. This book was released on 2016-01-06 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Main Focus of the book is: isolation of Lipase producing strains from various substrates. Using different screening methods identification of the hyper producer species for lipases. Optimization of culture conditions in solid state fermentation for selected strains. Extracellular lipase purification. Extracellular Lipase characterization in terms of temperature, pH, effects of detergents and metal ions effect. Identify the selected lipase producer strain, by ribo-typing. Application of lipases as bio-detergent and for bioremediation

Download or read book Optimization of Media Composition of Lipase Production from Oil Palm Press Fiber OPPF by Solid State Fermentation SSF written by Nor'Izzah Zainuddin and published by . This book was released on 2012 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipase is an enzyme which capable to be catalyst in the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. oil palm pressed fiber (OPPF) is an agricultural waste know as mesocarp fiber which recovered from pressed palm oil and based on dry weight basis, approximately it contains of 40% cellulose, 24% pentosan , 21% lignin and 5% ash. The production of extracellular lipase from OPPF using Aspergillus niger by solid state fermentation (SSF) was studied. The main target in this study is to produce high yield oflipase activity from OPPF based on media composition which is inducer, carbon and nitrogen sources.

Download or read book Optimization of Sustainable Enzymes Production written by J Satya Eswari and published by CRC Press. This book was released on 2022-11-29 with total page 239 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed as a reference book and presents a systematic approach to analyze evolutionary and nature-inspired population-based search algorithms. Beginning with an introduction to optimization methods and algorithms and various enzymes, the book then moves on to provide a unified framework of process optimization for enzymes with various algorithms. The book presents current research on various applications of machine learning and discusses optimization techniques to solve real-life problems. The book compiles the different machine learning models for optimization of process parameters for production of industrially important enzymes. The production and optimization of various enzymes produced by different microorganisms are elaborated in the book It discusses the optimization methods that help minimize the error in developing patterns and classifications, which further helps improve prediction and decision-making Covers the best-performing methods and approaches for optimization sustainable enzymes production with AI integration in a real-time environment Featuring valuable insights, the book helps readers explore new avenues leading towards multidisciplinary research discussions The book is aimed primarily at advanced undergraduates and graduates studying machine learning, data science and industrial biotechnology. Researchers and professionals will also find this book useful.

Download or read book Optimization of Process Conditions of Lipase Production from Coconut Dregs by Solid state Fermentation written by Nittiyah Vasudevan and published by . This book was released on 2013 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases comprise a group of hydrolytic enzymes which catalyze the hydrolysis and synthesis of triacylglycerides in the oil water interface. Screening of microorganism for extracellular lipase production from coconut dregs was conducted by using Aspergillus niger and Rhizopus oligosporus. The result shows that Rhizopus oligosporus able to produce higher lipase, 0.1527 U/mL compared to Aspergillus niger and thus it was chosen for further analysis. Process conditions such as pH, temperature and moisture content which influence the growth and production of lipase were studied. Central-Composite Design (CCD) of Response Surface approach was used to analyze the results and the optimum conditions for lipase production was determined. According to CCD, the optimum process conditions for Rhizopus oligosporus to produce lipase were as follow: 34.8 C, pH 5.24 and 62 % of moisture content. Validation process was carried out for 10 days under these conditions and it gives the maximum lipase activity at day 6 (0.2281 U/mL) . The standard error between optimization and validation was 2.23 %.

Download or read book The Production of Extracellular Protease Using Bacillus Subtilis written by Mohd Fadli Abdullah and published by . This book was released on 2006 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: Production of extracellular protease refers to proteases produced from microorganisms and functions out of the cell in which it was originated. In this term, the microorganism was sought to be normal mesophilic bacteria. The experiment was conducted to evaluate the effects of temperature and agitation speed of the incubator shaker towards the production of extracellular protease. Optimization of the enzyme production condition involving the two parameters was also made using statistical software. Typical methods conducted in previous researches were utilized in the experiments using different temperature and agitation speed. However, in previous researches, experiments were conducted through one-factor-at-a-time method (OF AT) but in this experiment, that method was overrun and designed beforehand using Minitab. The standard procedures of protease assay were carried out when experimenting. The bacteria were re-cultured to enable its functionality to serve its purpose. The results obtained proved that both parameters had significant effects if stood alone towards the production, however had to interact with each other due to randomization of the values implemented in the experiments. Simple analysis was not an option therefore the results were opted to be analyzed by Minitab and also the condition of protease production was optimized.

Download or read book Lipases written by Paul Woolley and published by Cambridge University Press. This book was released on 2012-01-26 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.

Download or read book Advances in Solid State Fermentation written by S. Roussos and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 641 pages. Available in PDF, EPUB and Kindle. Book excerpt: G.HAINNAUX Departement Milieu et Activites Agricoles, Centre ORSTOM, 911 Avenue d' Agropolis, B.P. 5045, 34032 Montpellier Cedex , France. Solid state fermentation, popularly abbreviated as SSF, is currently investigated by many groups throughout the world. The study of this technique was largely neglected in the past in European and Western countries and there is now a high demand for SSF, meaning in food, environment, agricultural, phannaceutical and many other biotechnological applications. It gives me satisfaction to note that the importance of this technique was realised at my department way back in 1975 since then, our team has put concentrated efforts on developing this technique. xvii Foreword Advances in Solid State Fermentation Foreword M. PUYGRENIER Agropolis Valorisation, Avenue d' Agropolis, 34394 Montpellier Cedex 5, France. On the name of the Scientific Community, I would like to express the wish that this International Symposium on SSF should be successful. Solid State Fermentation is part of biotechnology research. It consists on seeding solid culture medium with bacteria or fungi (filamentous or higher) and on producing, in this medium (solid components and exudates) metabolites and high value products. In fact, this process is very old. In older industries such the food and agricultural, this technique has been extensively used. An example of this is the production of pork sausages and Roquefort cheese. Pharmaceutical industry could make extensive use of SSF in the production of secondary metabolites of many kinds and development in this direction is soon expected.

Download or read book Solid state Fermentation written by Ashok Pandey and published by . This book was released on 1994 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Papers presented at Specialist Group Meeting & Symposium on Solid State Fermentation, held at Trivandrum, during March 23-24, 1994, organized by the Regional Research Laboratory, Trivandrum.

Download or read book Current Developments in Solid state Fermentation written by Ashok Pandey and published by Springer Science & Business Media. This book was released on 2008-09-16 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the period of last two decades, there has been significant resurgence in solid-state fermentation due to the numerous benefits it offers, especially in the engineering and environmental aspects. SSF has shown much promise in the development of several bioprocesses and products. This resurgence gained further momentum during the last 5-6 years with the developments in fundamental and applied aspects. A good deal of information has been generated in published literature and patented information. Several commercial ventures have come up based on SSF in different parts of the world. The contents are organized into four parts: Part 1 deals with the General and Fundamentals aspects of SSF; Part 2 deals with the production of bulk chemicals and products such as enzymes, organic acids, spores and mushrooms in SSF; Part 3 is on the use of SSF for specialty chemicals such as gibberellic acid, antibiotics and other pharmaceutically valuable secondary metabolites, pigments, and aroma compounds; Part 4 deals with the use of SSF miscellaneous application such as SSF for food and feed applications, agro-industrial residues as substrates in SSF and the production of silage and vermicompost.

Download or read book Liquid Biphasic System written by Pau Loke Show and published by Elsevier. This book was released on 2020-11-28 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings. Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. Provides information and examples of advanced separations in a single source Includes detailed descriptions of novel bioseparation systems Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

Download or read book Thermophilic Bacteria written by Jakob K. Kristjansson and published by CRC Press. This book was released on 2021-01-31 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermophilic Bacteria is a comprehensive volume that describes all major bacterial groups that can grow above 60-65°C (excluding the Archaea). Over 60 different species of aerobic and anaerobic thermophilic bacteria are covered. Isolation, growth methods, characterization and identification, ecology, metabolism, and enzymology of thermophilic bacteria are examined in detail, and an extensive compilation of recent biotechnological applications and the properties of many thermostable enzymes are also included. Major topics discussed in the book include a general review on thermophilic bacteria and archaea; heterotropic bacilli; the genus Thermus; new and rare genera of aerobic heterophophs, such as Saccharococcus, Rhodothermus, and Scotohermus; aerobic chemolithoautotrophic thermophilic bacteria; obligately anaerobic thermophilic bacteria; and hyperthermophilic Thermotogales and thermophilic phototrophs. Extensive bibliographies are also provided for each chapter. The vast amount of information packed into this one volume makes it essential for all microbiologists, biochemists, molecular biologists, and students interested in the expanding field of thermophilicity. Biotechnologists will find the book useful as a source of information on thermophiles or thermostable enzymes of possible industrial use.

Download or read book Fennema s Food Chemistry written by Srinivasan Damodaran and published by CRC Press. This book was released on 2017-05-25 with total page 1107 pages. Available in PDF, EPUB and Kindle. Book excerpt: This latest edition of the most internationally respected reference in food chemistry for more than 30 years, Fennema’s Food Chemistry, 5th Edition once again meets and surpasses the standards of quality and comprehensive information set by its predecessors. All chapters reflect recent scientific advances and, where appropriate, have expanded and evolved their focus to provide readers with the current state-of-the-science of chemistry for the food industry. This edition introduces new editors and contributors who are recognized experts in their fields. The fifth edition presents a completely rewritten chapter on Water and Ice, written in an easy-to-understand manner suitable for professionals as well as undergraduates. In addition, ten former chapters have been completely revised and updated, two of which receive extensive attention in the new edition including Carbohydrates (Chapter 3), which has been expanded to include a section on Maillard reaction; and Dispersed Systems: Basic considerations (Chapter 7), which includes thermodynamic incompatibility/phase separation concepts. Retaining the straightforward organization and accessibility of the original, this edition begins with an examination of major food components such as water, carbohydrates, lipids, proteins, and enzymes. The second section looks at minor food components including vitamins and minerals, colorants, flavors, and additives. The final section considers food systems by reviewing basic considerations as well as specific information on the characteristics of milk, the postmortem physiology of edible muscle, and postharvest physiology of plant tissues.

Download or read book Microbial Enzymes Roles and Applications in Industries written by Naveen Kumar Arora and published by Springer Nature. This book was released on 2020-04-28 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Microbial Enzymes: Roles and applications in industry” offers an essential update on the field of microbial biotechnology, and presents the latest information on a range of microbial enzymes such as fructosyltransferase, laccases, amylases, lipase, and cholesterol oxidase, as well as their potential applications in various industries. Production and optimisation technologies for several industrially relevant microbial enzymes are also addressed. In recent years, genetic engineering has opened up new possibilities for redesigning microbial enzymes that are useful in multiple industries, an aspect that the book explores. In addition, it demonstrates how some of the emerging issues in the fields of agriculture, environment and human health can be resolved with the aid of green technologies based on microbial enzymes. The topics covered here will not only provide a better understanding of the commercial applications of microbial enzymes, but also outline futuristic approaches to use microbial enzymes as driver of industrial sustainability. Lastly, the book is intended to provide readers with an overview of recent applications of microbial enzymes in various industrial sectors, and to pique researchers’ interest in the development of novel microbial enzyme technologies to meet the changing needs of industry.

Download or read book Current Developments in Biotechnology and Bioengineering written by Ashok Pandey and published by Elsevier. This book was released on 2017-09-12 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Developments in Biotechnology and Bioengineering: Current Advances in Solid-State Fermentation provides knowledge and information on solid-state fermentation involving the basics of microbiology, biochemistry, molecular biology, genetics and principles of genetic engineering, metabolic engineering and biochemical engineering. This volume of the series is on Solid-State fermentation (SSF), which would cover the basic and applied aspects of SSF processes, including engineering aspects such as design of bioreactors in SSF. The book offers a pool of knowledge on biochemical and microbiological aspects as well as chemical and biological engineering aspects of SSF to provide an integrated knowledge and version to the readers. Provides state-of-the-art information on basic and fundamental principles of solid-state fermentation Includes key features for the education and understanding of biotechnology education and R&D, in particular on SSF Lists fermentation methods for the production of a wide variety of enzymes and metabolites Provides examples of the various industrial applications of enzymes in solid state fermentation

Download or read book Bioprocessing for Biomolecules Production written by Gustavo Molina and published by John Wiley & Sons. This book was released on 2020-01-21 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents the many recent innovations and advancements in the field of biotechnological processes This book tackles the challenges and potential of biotechnological processes for the production of new industrial ingredients, bioactive compounds, biopolymers, energy sources, and compounds with commercial/industrial and economic interest by performing an interface between the developments achieved in the recent worldwide research and its many challenges to the upscale process until the adoption of commercial as well as industrial scale. Bioprocessing for Biomolecules Production examines the current status of the use and limitation of biotechnology in different industrial sectors, prospects for development combined with advances in technology and investment, and intellectual and technical production around worldwide research. It also covers new regulatory bodies, laws and regulations, and more. Chapters look at biological and biotechnological processes in the food, pharmaceutical, and biofuel industries; research and production of microbial PUFAs; organic acids and their potential for industry; second and third generation biofuels; the fermentative production of beta-glucan; and extremophiles for hydrolytic enzymes productions. The book also looks at bioethanol production from fruit and vegetable wastes; bioprocessing of cassava stem to bioethanol using soaking in aqueous ammonia pretreatment; bioprospecting of microbes for bio-hydrogen production; and more. Provides up to date information about the advancements made on the production of important biotechnological ingredients Complete visualization of the general developments of world research around diverse products and ingredients of technological, economic, commercial and social importance Investigates the use and recovery of agro-industrial wastes in biotechnological processes Includes the latest updates from regulatory bodies for commercialization feasibility Offering new products and techniques for the industrial development and diversification of commercial products, Bioprocessing for Biomolecules Production is an important book for graduate students, professionals, and researchers involved in food technology, biotechnology; microbiology, bioengineering, biochemistry, and enzymology.