Download or read book Portable Protocols Using Antibodies written by Edward Harlow and published by Cold Spring Harbor Laboratory Press. This book was released on 1998-12 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Few technical manuals have become standards in biomedicine. Antibodies: A Laboratory Manual, by Ed Harlow and David Lane, has had that status for a decade. Now there is a new and even higher standard -- Using Antibodies: A Laboratory Manual. Harlow and Lane have completely revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been entirely rewritten, reorganized, and updated to provide background, context, and step-by-step instructions for techniques that range from choosing the right antibody and handling it correctly, to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, Using Antibodies instead identifies in each case the best approach to specific problems. These recommendations include more detail in the protocols, extensive advice on avoiding and solving problems, information regarding proper controls, and extensive illustration of theory, methods, and results, both good and bad. An additional bonus included with this manual is a set of Portable Protocols, step-by-step instructions for the most frequently used and essential techniques printed on spill-proof, durable cards that can be annotated and used directly at the bench. The expert advice in Using Antibodies is presented using an imaginative design with extensive use of color and graphic elements calculated to help readers plan and execute their experiments efficiently and accurately. A newly available type of binding will maintain the manual's integrity during years of use. This new manual reflects a decade'sadditional research experience by two outstanding scientists of international reputation. Since writing the previous manual, Ed Harlow has received many awards, notably the General Motors and Bristol Myers prizes for cancer research, and he was elected to the National Academy of Sciences. David Lane, also the winner of many awards, such as the Yvette Mayent Prize and the Paul Ehrlich and Ludwig Darmstaedter Award, was elected as a fellow of the Royal Society. The over-the-shoulder advice these experts provide in Using Antibodies will lead all laboratory investigators to success in using these techniques, regardless of experience. Using Antibodies is a required resource for every laboratory in which genes, cells, and proteins are studied.
Download or read book Using Antibodies written by Edward Harlow and published by . This book was released on 1999 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Few technical manuals have become standards in biomedicine. Antibodies: A Laboratory Manual, by Ed Harlow and David Lane, has had that status for a decade. Now there is a new and even higher standard -- Using Antibodies: A Laboratory Manual. Harlow and Lane have completely revised their guide to the use of immunoglobulin reagents in the laboratory. Chapters have been entirely rewritten, reorganized, and updated to provide background, context, and step-by-step instructions for techniques that range from choosing the right antibody and handling it correctly, to the proper methods for characterizing antigens in cells and solutions. New chapters on tagging proteins and epitope mapping are included. Rather than presenting an array of solutions for working with antibodies and antigens, Using Antibodies instead identifies in each case the best approach to specific problems. These recommendations include more detail in the protocols, extensive advice on avoiding and solving problems, information regarding proper controls, and extensive illustration of theory, methods, and results, both good and bad. An additional bonus included with this manual is a set of Portable Protocols, step-by-step instructions for the most frequently used and essential techniques printed on spill-proof, durable cards that can be annotated and used directly at the bench. The expert advice in Using Antibodies is presented using an imaginative design with extensive use of color and graphic elements calculated to help readers plan and execute their experiments efficiently and accurately. A newly available type of binding will maintain the manual's integrity during years of use. This new manual reflects a decade'sadditional research experience by two outstanding scientists of international reputation. Since writing the previous manual, Ed Harlow has received many awards, notably the General Motors and Bristol Myers prizes for cancer re
Download or read book Using The Biological Literature written by Diane Schmidt and published by CRC Press. This book was released on 2001-12-06 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in th
Download or read book Making and Using Antibodies written by Gary C. Howard and published by CRC Press. This book was released on 2006-12-13 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scienti
Download or read book Monoclonal Antibody Protocols written by William C. Davis and published by . This book was released on 1995 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monoclonal Antibody Protocols provides researchers in biomedical, agricultural, and biological science with a set of detailed, easy-to-follow methods for developing and using monoclonal antibodies. The protocols emphasize techniques that optimize the outgrowth of hybridomas from primary cultures of fused cells and the use of an alternative, electric-field-mediated cell fusion technique to increase the yield of hybridomas. The book stresses antibodies produced in mice, but includes methods of producing xenogeneic hybrids that yield human, bovine, equine, and porcine monoclonal antibodies. With its detailed instructions, its comments on how to alter the various steps of a protocol in order to accommodate different materials, and its hints and tips that often make the difference between success and failure, Monoclonal Antibody Protocols provides you with a ready and indispensable source of information for preparing and using monoclonal antibodies successfully in your laboratory.
Download or read book Antibody Methods and Protocols written by Gabriele Proetzel and published by Humana Press. This book was released on 2016-05-01 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Methods in Molecular Biology volume covers in vitro and in vivo generation of antibodies, as well as techniques for screening, analysis and modification of antibodies and antibody fragments. Offers materials lists, protocols and troubleshooting tips."
Download or read book Antibody Engineering Protocols written by Sudhir Paul and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.
Download or read book Mobile Diagnosis 2 0 written by Aniruddha Ray and published by MDPI. This book was released on 2021-06-04 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mobile sensing and diagnostic capabilities are becoming extremely important for a wide range of emerging applications and fields spanning mobile health, telemedicine, point-of-care diagnostics, global health, field medicine, democratization of sensing and diagnostic tools, environmental monitoring, and citizen science, among many others. The importance of low-cost mobile technologies has been underlined during this current COVID-19 pandemic, particularly for applications such as the detection of pathogens, including bacteria and viruses, as well as for prediction and management of different diseases and disorders. This book focuses on some of these application areas and provides a timely summary of cutting-edge results and emerging technologies in these interdisciplinary fields.
Download or read book Antibody Techniques written by Vedpal S. Malik and published by Academic Press. This book was released on 2013-10-22 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Detailed, easy-to-follow, step-by-step protocols Convenient, easy-to-use format Extensive practical information Essential background information Helpful hints
Download or read book Immunochemical Protocols written by Robert Burns and published by Humana. This book was released on 2010-11-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunochemical techniques have been in use for many years with early examples of bacterial strain typing dating back to the 1940s. The basis for the science is the exquisite elegance of the mammalian immune system with its ability to recognize foreign proteins and to manufacture antibody m- ecules that strongly bind to the substances that elicited them. Not only are potentially harmful pathogens and toxins recognized by the immune system, but the system can be persuaded to manufacture antibodies to an astonishing array of substances. In the early days of this science, all antibodies for investigative work were produced by immunizing mammals with the substance of interest, followed by regular donor bleeds that yielded antisera. Serum produced in this way yields heterogenic populations of antibody molecules recognizing different epitopes on the target protein, which may be adequate for its intended p- poses, but can also cause problems of crossreactivity. In 1975, Kohler and Milstein reported that spleen cells from immune donor animals could be immortalized, cloned from single cells, and grown in continuous culture. This original work described the method for the production of monoclonal antibodies.
Download or read book Epitope Mapping Protocols written by Glenn E. Morris and published by Springer Science & Business Media. This book was released on 1996-08 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epitope Mapping Protocols offers more than thirty protocols for antibody production and mapping-all described in detail by leading experts and illustrated by proven examples from their own work. The methods range from those that use (or can use) whole, native antigens to those that use peptides and peptide libraries and those that require antigen expressed from recombinant DNA. A method for T-cell epitopes, instructions on using "natural" variants or mutants, and techniques for producing panels of antibodies for epitope mapping are also included.
Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Download or read book Rapid Antibody based Technologies in Food Analysis written by Richard O'Kennedy and published by Royal Society of Chemistry. This book was released on 2019-06-19 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are significant challenges in food analysis, problems with food contamination and authentication, and a worldwide need to ensure food safety. This book provides a description of antibody-based technologies used in food analysis. It focuses on key applications, outlining the approaches used, their advantages and limitations, and describes future areas for development. Chapters are written by experts in the field, critically examining each of the currently used methodologies and highlighting new evolving technologies, such as lab-on-chip and microfluidics-based devices and biosensors. Case studies demonstrating the utility of each of the methods described are included. Important introductory chapters cover sample preparation for analysis and statistical sampling necessary for quality control for verification of results. An overview chapter highlighting major analytical issues and areas that have specific requirements, e.g. food authentication, is provided. Researchers and scientists in the field who have to acquire, verify and use technologies for food analysis, food producers and processors, food safety and testing laboratories, and government agencies will all find this a useful addition to their library.
Download or read book Therapeutic Antibodies written by Gunnar Houen and published by Humana. This book was released on 2022-09-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Antibodies: Methods and Protocols serves as an ideal guide for researchers working with the production of, research on, and development of therapeutic antibodies as well as for clinicians using therapeutic antibodies in daily work with patients.
Download or read book Single Domain Antibodies written by Greg Hussack and published by Springer Nature. This book was released on 2022-02-14 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume covers current and emerging techniques for studying single-domain antibodies (sdAbs). Chapters guide readers through the biology and immunology of sdAbs in camelids and sharks, isolation of sdAbs, protein engineering approaches to optimize the solubility, stability, valency and antigen binding affinity of sdAbs, and specialized applications of sdAbs. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Single-Domain Antibodies: Methods and Protocols aims to be a useful, practical guide to help researchers further their studies in this field.
Download or read book BioSupplyNet Source Book written by and published by . This book was released on 1999 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Journal of Cell Biology written by and published by . This book was released on 1998 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: No. 2, pt. 2 of November issue each year from v. 19-47; 1963-70 and v. 55- 1972- contain the Abstracts of papers presented at the annual meeting of the American Society for Cell Biology, 3d-10th; 1963-70 and 12th- 1972- .
