Download or read book Phenotypic characterization of temperature sensitive cell division mutants of Escherichia coli K 12 written by Robert George Allen and published by . This book was released on 1974 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and genetic mapping of temperature sensitive cell division mutants of Escherichia coli K 12 written by Jane Ann Smith Allen and published by . This book was released on 1974 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of a Temperature sensitive Mutation in the 88 89 Minutes of the Escherichia Coli Chromosome written by Issmat Ibrahim Kassem and published by . This book was released on 2003 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: In an attempt to isolate a temperature sensitive allele of ftsN, chemical random mutagenesis was performed using hydroxylamine. SMM 12 a temperature sensitive filamentous strain was successfully isolated. However, it did not carry an ftsN(Ts) allele. Transforming with different pasmids carrying cloned genes and random genomic plasmid libraries have yielded no complementation. Using P I -mediated transduction, the location of the mutation was found to be at the 88-89 minute region on the E. coli chromosome. This finding represents the discovery of an unprecedented gene at the 88- 89 minute region that blocks cell division. in addition, we report in this work the isolation of the first temperature-sensitive mutant that is suppressed by L-glutamate. Although the precise identity of the mutated gene remains illusive, all data produced by this work point out that we may have isolated a previously unknown player in the cell- division protein machinery in E. coli.

Download or read book The Isolation and Partial Characterization of Some Mutants of Escherichia Coli K 12 with Temperature sensitive Synthesis of DNA written by Philip Louis Carl and published by . This book was released on 1968 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Analysis and Phenotypic Characterization of Lon Mutants of Escherichia Coli K 12 written by Angel S. Torres-Cabassa and published by . This book was released on 1982 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Isolation and Characterization of Temperature sensitive Polynucleotide Phosphorylase Mutants of Escherichia Coli K 12 written by Frances Verdenal Meffen and published by . This book was released on 1981 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Eight Temperature Sensitive Mutants of Escherichia Coli by Genetic Analysis and Molecular Cloning written by Katherine Armstrong and published by . This book was released on 1981 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Temperature sensitive FtsZ Alleles that Modulate the Rod to Sphere Morphogenesis in Escherichia Coli written by Ziad Walid El-Hajj and published by . This book was released on 2005 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: The normally rod-shaped bacterium Escherichia coli K-12 divides by elongating and then forming a septum at midcell, which will result in the format ion of two daughter cells. Septation is driven by the polymerization of the essential d ivision protein FtsZ into a structure called the Z-ring, and the subsequent recruitment of other cell division proteins to form the divisome. Temperature-sensitive (Ts) ftsZ mut ants can divide as rods at 30 degreesC but filament and lyse at 42 degreesC, and are ther efore used as tools to study the role of FtsZ in cell division. The rod shape of E. coli is maintained by the pbpA gene product Penicillin-Binding Protein 2 (PBP2), and upon inactivation of pbpA the cells become spherical, fail to divide, and eventually die. Simultaneous overexpression of the essential cell division genes ftsQ, ftsA and ftsZ from the lowcopy- number plasmid pBS58 restores division in spherical cells on LB agar 30 degreesC and 37 degreesC but not 42 degreesC. Several temperature-sensitive (Ts) ftsZ mutants were tested for their ability to support division in spheres, with the mutation being either on the chromosome, o n a pBS58 derivative, or both. At the permissive temperature of 30degreesC, ftsZ84 s upported division in all combinations. On the other hand, ftsZ972, ftsZ2066, ftsZ2863, fts Z6460 and ftsZ9124 supported division and viability in spherical cells only when prese nt in combination with wild-type ftsZ. Furthermore, an ftsZ26 mutant carrying pBS58 co uld divide more efficiently as a sphere than a rod. Overexpression of ftsWin additio n to ftsQAZ recovered spherical division in ftsZ972 but had no effect on the other mu tants. Surprisingly, the ftsZ26, ftsZ84, ftsZ2863 and ftsZ6460 mutations in combination with wild-type ftsZ supported division in spheres at the non-permissive temperature. These data demonstrate that in addition to conferring temperature-sensitivity, some ft sZ(Ts) mutants impair cell division lethally at the permissive temperature in spherical but not rod-shaped E. coli, and a few of these as well as other mutants suppress the dea th of spherical cells at the non-permissive temperature. Sphere-dependent phenotypes o f ftsZ(Ts) alleles are likely to reflect subtle effects on key events during cell division, including FtsZ polymerization and/or its interactions with other cell division p ...

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Two component Signal Transduction written by James A. Hoch and published by Amer Society for Microbiology. This book was released on 1995 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human enteroviruses, particularly the polio viruses, have had a significant role in the history of medicine and microbiology; and continue to cause clinical problems, as well as provide targets for molecular investigation. This book offers a link between the basic science and clinical medicine.

Download or read book Journal of Bacteriology written by Charles-Edward Amory Winslow and published by . This book was released on 1982 with total page 1260 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetics of Adaptation written by Rodney Mauricio and published by Springer Science & Business Media. This book was released on 2005-07-20 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: An enduring controversy in evolutionary biology is the genetic basis of adaptation. Darwin emphasized "many slight differences" as the ultimate source of variation to be acted upon by natural selection. In the early 1900’s, this view was opposed by "Mendelian geneticists", who emphasized the importance of "macromutations" in evolution. The Modern Synthesis resolved this controversy, concluding that mutations in genes of very small effect were responsible for adaptive evolution. A decade ago, Allen Orr and Jerry Coyne reexamined the evidence for this neo-Darwinian view and found that both the theoretical and empirical basis for it were weak. Orr and Coyne encouraged evolutionary biologists to reexamine this neglected question: what is the genetic basis of adaptive evolution? In this volume, a new generation of biologists have taken up this challenge. Using advances in both molecular genetic and statistical techniques, evolutionary geneticists have made considerable progress in this emerging field. In this volume, a diversity of examples from plant and animal studies provides valuable information for those interested in the genetics and evolution of complex traits.

Download or read book Brazilian Journal of Genetics written by and published by . This book was released on 1989 with total page 1040 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1975 with total page 744 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of the Fission Yeast written by and published by Elsevier. This book was released on 2012-12-02 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism.

Download or read book Dissertation Abstracts International written by and published by . This book was released on 1991 with total page 774 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Comprehensive Dissertation Index written by and published by . This book was released on 1984 with total page 856 pages. Available in PDF, EPUB and Kindle. Book excerpt: