Download or read book Organization of T4 Bacteriophage Genes and Gene Products Involved in DNA Precursor Biosynthesis written by Yeong Wang and published by . This book was released on 1989 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteriophage T4 gene 42 encodes dCMP hydroxymethylase, an enzyme unique to the deoxyribonucleotide metabolism of T-even bacteriophages. To study biochemical and biophysical properties of the enzyme, as well as the interaction of dCMP hydroxymethylase with other DNA precursor biosynthetic enzymes in vitro, availability of large amounts of the enzyme is necessary. Therefore, cloning and overexpression of T4 gene 42 are needed for these studies. An 1.8-kb Eco RI restriction fragment of a T4 multiple mutant BK536, containing an amber mutation in gene 42, has been cloned into pUC19 plasmid vector in Escherichia. coil. Genetic and biochemical examination of the cloned T4 DNA fragment has revealed that it contains the entire gene 42 coding sequence. The cloned amber mutant gene was converted to a wild type gene by site-directed mutagenesis, and the wild type gene 42 was overexpressed with the pT7 expression vector system. dCMP hydroxymethylase expressed from the cloned gene 42 was purified to homogeneity. The specific activity and N-terminal amino acid sequence of the purified enzyme were determined. The specific activity of the purified cloned gene product closely agreed with the value of the enzyme purified- from phage-infected E. colt cells. Nterminal amino acid determination has confirmed the open reading frame of gene 42, deduced from nucleotide sequence of gene 42. Purified dCMP hydroxymethylase has also been used to study its crystal structure and catalytic mechanism by collaboration with other research groups. In addition, purified dCMP hydroxymethylase was immobilized on Affi-Gel to prepare an affinity column, which was used to study protein-protein interaction among dNTP biosynthetic enzymes and with other DNA metabolic proteins. The proteins which specifically interact with dCMP hydroxymethylase were identified by SDS polyacrylamide gel electrophoresis followed by fluorography, Western blotting, and two dimensional gel electrophoresis. It was earlier found that T4-encoded thymidylate synthase and dihydrofolate reductase function not only in deoxyribonucleotide biosynthesis but are also structural components of the phage baseplate. Two deletion mutants containing deletion in the td gene, encoding thymidylate synthase, and the frd gene, encoding dihydrofolate reductase, were carefully characterized, and used to reevaluate structural role of these two enzymes.
Download or read book Bacteriophage T4 written by Christopher K. Mathews and published by . This book was released on 1983 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of Bacteriophage T4 written by Jim D. Karam and published by ASM Press. This book was released on 1994 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new text highlights the value of this biological system as a research and teaching tool. The book is a sequel to the 1983 edition and is organized into 6 major sections: DNA metabolism, regulation of gene expression, morphogenesis, structure of selected proteins, host–phage interactions, and laboratory experiments in T4 molecular genetics. Since T4 has played a central role in the development of molecular biology as an academic discipline, the themes presented in this book provide a framework for designing graduate and undergraduate courses in prokaryotic genetics and biochemistry.
Download or read book DNA Synthesis and the Expression of Two Classes of Late Genes of Bacteriophage T4 written by Robert David Bruner and published by . This book was released on 1969 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Host Kinases Involved in DNA Precursor Biosynthesis During Bacteriophage T4 Infection written by Mark Aguirre Bernard and published by . This book was released on 1998 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Gol Site written by Wendy Cooley Champness and published by . This book was released on 1982 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies on the Biological Role and Genetics of the T4 Bacteriophage RNA Ligase written by Judith Marie Runnels and published by . This book was released on 1981 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phage and the Origins of Molecular Biology written by John Cairns and published by CSHL Press. This book was released on 2007 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1966 as a 60th birthday tribute to Max Delbrck, this influential work is republished as "The Centennial Edition." The book was hailed as "[introducing] into the literature of science, for the first time, a self-conscious historical element in which the participants in scientific discovery engage in writing their own chronicle ("Journal of History of Biology").
Download or read book Bacteriophages written by David R. Harper and published by Springer Nature. This book was released on 2021-01-30 with total page 1376 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first major reference work dedicated to the mannifold industrial and medical applications of bacteriophages provides both theoretical and practical insights into the emerging field of bacteriophage biotechnology. The book introduces to bacteriophage biology, ecology and history and reviews the latest technologies and tools in bacteriophage detection, strain optimization and nanotechnology. Usage of bacteriophages in food safety, agriculture, and different therapeutic areas is discussed in detail. This book serves as essential guide for researchers in applied microbiology, biotechnology and medicine coming from both academia and industry.
Download or read book Modern Microbial Genetics written by Uldis N. Streips and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: In accordance with its predecessor, the completely revised and expanded Second Edition of Modern Microbial Genetics focuses on how bacteria and bacteriophage arrange and rearrange their genetic material through mutation, evolution, and genetic exchange to take optimal advantage of their environment. The text is divided into three sections: DNA Metabolism, Genetic Response, and Genetic Exchange. The first addresses how DNA replicates, repairs itself, and recombines, as well as how it may be manipulated. The second section is devoted to how microorganisms interact with their environment, including chapters on sporulation and stress shock, and the final section contains the latest information on classic exchange mechanisms such as transformation and conjugation. Chapters include: * Gene Expression and Its Regulation * Single-Stranded DNA Phages * Genetic Tools for Dissecting Motility and Development of Myxococcus xanthus * Molecular Mechanism of Quorum Sensing * Transduction in Gram-Negative Bacteria * Genetic Approaches in Bacteria with No Natural Genetic Systems The editors also cultivate an attention to global regulatory systems throughout the book, elucidating how certain genes and operons in bacteria, defined as regulons, network and cooperate to suit the needs of the bacterial cell. With clear appreciation for the impact of molecular genomics, this completely revised and updated edition proves that Modern Microbial Genetics remains the benchmark text in its field.
Download or read book Recombinational Repair of DNA Damage written by Andrei Kuzminov and published by Landes Bioscience. This book was released on 1996 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Faculties Publications and Doctoral Theses in Chemistry and Chemical Engineering at United States Universities written by American Chemical Society. Committee on Professional Training and published by . This book was released on 1991 with total page 1646 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Viral Molecular Machines written by Michael G. Rossmann and published by Springer Science & Business Media. This book was released on 2012-02-02 with total page 685 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will contain a series of solicited chapters that concern with the molecular machines required by viruses to perform various essential functions of virus life cycle. The first three chapters (Introduction, Molecular Machines and Virus Architecture) introduce the reader to the best known molecular machines and to the structure of viruses. The remainder of the book will examine in detail various stages of the viral life cycle. Beginning with the viral entry into a host cell, the book takes the reader through replication of the genome, synthesis and assembly of viral structural components, genome packaging and maturation into an infectious virion. Each chapter will describe the components of the respective machine in molecular or atomic detail, genetic and biochemical analyses, and mechanism. Topics are carefully selected so that the reader is exposed to systems where there is a substantial infusion of new knowledge in recent years, which greatly elevated the fundamental mechanistic understanding of the respective molecular machine. The authors will be encouraged to simplify the detailed knowledge to basic concepts, include provocative new ideas, as well as design colorful graphics, thus making the cutting-edge information accessible to broad audience.
Download or read book Molecular Evolution written by Roderick D.M. Page and published by John Wiley & Sons. This book was released on 2009-07-14 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of evolution at the molecular level has given the subject of evolutionary biology a new significance. Phylogenetic 'trees' of gene sequences are a powerful tool for recovering evolutionary relationships among species, and can be used to answer a broad range of evolutionary and ecological questions. They are also beginning to permeate the medical sciences. In this book, the authors approach the study of molecular evolution with the phylogenetic tree as a central metaphor. This will equip students and professionals with the ability to see both the evolutionary relevance of molecular data, and the significance evolutionary theory has for molecular studies. The book is accessible yet sufficiently detailed and explicit so that the student can learn the mechanics of the procedures discussed. The book is intended for senior undergraduate and graduate students taking courses in molecular evolution/phylogenetic reconstruction. It will also be a useful supplement for students taking wider courses in evolution, as well as a valuable resource for professionals. First student textbook of phylogenetic reconstruction which uses the tree as a central metaphor of evolution. Chapter summaries and annotated suggestions for further reading. Worked examples facilitate understanding of some of the more complex issues. Emphasis on clarity and accessibility.
Download or read book An Introduction to Genetic Engineering written by Desmond S. T. Nicholl and published by Cambridge University Press. This book was released on 2002-02-07 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: The author presents a basic introduction to the world of genetic engineering. Copyright © Libri GmbH. All rights reserved.
Download or read book Basic Virology written by Martinez J. Hewlett and published by John Wiley & Sons. This book was released on 2021-04-27 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: The foundational textbook on the study of virology Basic Virology, 4th Edition cements this series’ position as the leading introductory virology textbook in the world. It’s easily read style, outstanding figures, and comprehensive coverage of fundamental topics in virology all account for its immense popularity. This undergraduate-accessible book covers all the foundational topics in virology, including: The basics of virology Virological techniques Molecular biology Pathogenesis of human viral disease The 4th edition includes new information on the SARS, MERS and COVID-19 coronaviruses, hepatitis C virus, influenza virus, as well as HIV and Ebola. New virological techniques including bioinformatics and advances in viral therapies for human disease are also explored in-depth. The book also includes entirely new sections on metapneumoviruses, dengue virus, and the chikungunya virus.
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
