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- Release : 2017
- ISBN :
- Pages : 68 pages
Optimization of Biophotonics and Optomechanics for Frequency Domain Tissue Fluorescence Measurements
Download or read book Optimization of Biophotonics and Optomechanics for Frequency Domain Tissue Fluorescence Measurements written by and published by . This book was released on 2017 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: Collagens and extra cellular matrix (ECM) proteins play important roles in the structural composition of tissues, and their modifications are implicated in diseases including cancer metastasis, tissue fibrosis, diabetic ulcers. Current tissue collagen analyses are based on destructive histological methods, whereas the ECM proteins' intrinsic autofluorescences can provide a label-free detection and imaging to differentiate tissue compositions, modifications, and disease states. Fluorescence lifetime spectroscopy has been demonstrated as a key technique to distinguish ECM proteins in tissue. However, the large, expensive, and often operationally challenging instrumentation required to measure fluorescent lifetimes from tissues prevents the technique from gaining traction in biomedical applications. By providing a quantitative, more nuanced model of tissue biophotonics while using low-cost, low complexity FD instrumentation, the works in this thesis hopes to increase accessibility and popularity of label-free ECM lifetime spectroscopy for a number of biomedical and clinical applications.