Download or read book Nano and Micro scale Temperature Measurements Using Laser induced Fluorescence Thermometry written by Christine Tomforde and published by . This book was released on 2011 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: A method of determining nanoparticle temperature through fluorescence intensity levels is described. Intracellular processes are often tracked through the use of fluorescence tagging, and ideal temperatures for many of these processes are unknown. Through the use of fluorescence-based thermometry, cellular processes such as intracellular enzyme movement can be studied and their respective temperatures established simultaneously. Polystyrene and silica nanoparticles are synthesized with a variety of temperature-sensitive dyes such as BODIPY, rose Bengal, Rhodamine dyes 6G, 700, and 800, and Nile Blue A and Nile Red. Photographs are taken with a QImaging QM1 Questar EXi Retiga camera while particles are heated from 25 to 70 C and excited at 532 nm with a Coherent DPSS-532 laser. Photographs are converted to intensity images in MATLAB and analyzed for fluorescence intensity, and plots are generated in MATLAB to describe each dye's intensity vs temperature. Regression curves are created to describe change in fluorescence intensity over temperature. Dyes are compared as nanoparticle core material is varied. Large particles are also created to match the camera's optical resolution capabilities, and it is established that intensity values increase proportionally with nanoparticle size. Nile Red yielded the closest-fit model, with R2 values greater than 0.99 for a second-order polynomial fit. By contrast, Rhodamine 6G only yielded an R2 value of 0.88 for a third-order polynomial fit, making it the least reliable dye for temperature measurements using the polynomial model. Of particular interest in this work is Nile Blue A, whose fluorescence-temperature curve yielded a much different shape from the other dyes. It is recommended that future work describe a broader range of dyes and nanoparticle sizes, and use multiple excitation wavelengths to better quantify each dye's quantum efficiency. Further research into the effects of nanoparticle size on fluorescence intensity levels should be considered as the particles used here greatly exceed 2 ìm. In addition, Nile Blue A should be further investigated as to why its fluorescence-temperature curve did not take on a characteristic shape for a temperature-sensitive dye in these experiments.

Download or read book Application of a Ratiometric Laser Induced Fluorescence LIF Thermometry for Micro scale Temperature Measurement for Natural Convection Flows written by Heon Ju Lee and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A ratiometric laser induced fluorescence (LIF) thermometry applied to micro-scale temperature measurement for natural convection flows. To eliminate incident light non-uniformity and imperfection of recording device, two fluorescence dyes are used: one is temperature sensitive fluorescence dye (Rhodamine B) and another is relatively temperature insensitive fluorescence dye (Rhodamine 110). Accurate and elaborate calibration for intensity ratio verses temperature obtained using an isothermal cuvette, which was controlled by two thermo-bathes. 488nm Ar-ion laser used for incident light and two filter sets used for separating each fluorescence emission. The several difficulties for applying to heated evaporating meniscus were identified and a few resolutions were suggested.

Download or read book Thermometry at the Nanoscale written by Luís Dias Carlos and published by Royal Society of Chemistry. This book was released on 2016 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamentals of measuring temperature at the nanoscale, luminescence-based and non-luminescence based thermometry techniques, and applications.

Download or read book Microscale Observables for Heat and Mass Transport in Sub micron Scale Evaporating Thin Film written by Sang-Kwon Wee and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A mathematical model is developed to describe the micro/nano-scale fluid flow and heat/mass transfer phenomena in an evaporating extended meniscus, focusing on the transition film region under nonisothermal interfacial conditions. The model incorporates thermocapillary stresses at the liquid-vapor interface, a slip boundary condition on the solid wall, polarity contributions to the working fluid field, and binary mixture evaporation. The analytical results show that the adsorbed film thickness and the thin film length decrease with increasing superheat by the thermocapillary stresses, which influences detrimentally the evaporation process by degrading the wettability of the evaporating liquid film. In contrast, the slip effect and the binary mixture enhance the stability of thin film evaporation. The slip effect at the wall makes the liquid in the transition region flow with smaller flow resistance and thus the length of the transition region increases. In addition, the total evaporative heat flow rate increases due to the slip boundary condition. The mixture of pentane and decane increases the length of the thin film by counteracting the thermocapillary stress, which enhances the stability of the thin film evaporation. The polarity effect of water significantly elongates the thin film length due to the strong adhesion force of intermolecular interaction. The strong interaction force restrains the liquid from evaporation for a polar liquid compared to a non-polar liquid. In the experimental part, laser induced fluorescence (LIF) thermometry has been used to measure the microscale temperature field of a heated capillary tube with a 1 mm by 1 mm square cross section. For the temperature measurement, the calibration curve between the temperature and the fluorescent intensity ratio of Rhodamine-B and Rhodamine-110 has been successfully obtained. The fluorescent intensity ratio provides microscale spatial resolution and good temperature dependency without any possible bias error caused by illuminating light and background noise usually encountered in conventional LIF techniques. For the validation of the calibration curve obtained, thermally stratified fields established inside a glass cuvette of 10 mm width were measured. The measurement result showed a good agreement with the linear prediction. The temperature measurement in a 1 mm capillary tube could provide the feasible method of temperature measurement for the thin film region in the future.

Download or read book Surface Thermometry of Energetic Materials by Laser Induced Fluorescence written by Larry P. Goss and published by . This book was released on 1988 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt: The laser-induced fluorescence from Dy: YAG has been employed for t he measurement of temperature on reacting and nonreacting surfaces. Point, line, and two-dimensional mapping of the surface temperature has been demonstrated utilizing the tripled output (355 nm) of a Nd:YAG laser. The temporal and spatial distribution of the temperature was recorded with a two-dimensional intensified imaging system. The technique has also been utilized for the measurement of the thermal depth profile of a plastic material undergoing rapid heating by a CO2 laser. A fiber-optic temperature probe was developed. Fluorescent crystals bonded to the fiber-optic tip with a ceramic adhesive provide a 300 - 1100 K temperature range for the probe. Surface temperature measurement, Laser-induced fluorescence, Solid-fuel propellants, Rare-earth ions, Nonintrusive evaluation, Optical diagnostics. (jes).

Download or read book Measurement of Temperature on In flight Water Droplets by Laser Induced Fluorescence Thermometry written by Víctor M. Salazar Izquierdo and published by . This book was released on 2003 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Intracellular Thermometry with Fluorescent Molecular Thermometers written by Seiichi Uchiyama and published by John Wiley & Sons. This book was released on 2024-06-17 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Download or read book Heat Conduction Using Green s Functions written by Kevin Cole and published by Taylor & Francis. This book was released on 2010-07-16 with total page 666 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its publication more than 15 years ago, Heat Conduction Using Green's Functions has become the consummate heat conduction treatise from the perspective of Green's functions-and the newly revised Second Edition is poised to take its place. Based on the authors' own research and classroom experience with the material, this book organizes the so

Download or read book Optofluidic Devices and Applications written by Francisco Yubero and published by MDPI. This book was released on 2020-12-23 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optofluidic devices are of high scientific and industrial interest in chemistry, biology, material science, pharmacy, and medicine. In recent years, they have experienced strong development because of impressive achievements in the synergistic combination of photonics and micro/nanofluidics. Sensing and/or lasing platforms showing unprecedented sensitivities in extremely small analyte volumes, and allowing real-time analysis within a lab-on-a-chip approach, have been developed. They are based on the interaction of fluids with evanescent waves induced at the surface of metallic or photonic structures, on the implementation of microcavities to induce optical resonances in the fluid medium, or on other interactions of the microfluidic systems with light. In this context, a large variety of optofluidic devices has emerged, covering topics such as cell manipulation, microfabrication, water purification, energy production, catalytic reactions, microparticle sorting, micro-imaging, or bio-sensing. Moreover, the integration of these optofluidic devices in larger electro-optic platforms represents a highly valuable improvement towards advanced applications, such as those based on surface plasmon resonances that are already on the market. In this Special Issue, we invited the scientific community working in this rapidly evolving field to publish recent research and/or review papers on these optofluidic devices and their applications.

Download or read book Luminescence Thermometry written by Miroslav Dramićanin and published by Woodhead Publishing. This book was released on 2018-04-21 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Luminescence Thermometry: Methods, Materials, and Applications presents the state-of-the art applications of luminescence thermometry, giving a detailed explanation of luminescence spectroscopic schemes for the read-out of temperature, while also describing the diverse materials that are capable of sensing temperature via luminescence. Chapters cover the fundamentals of temperature, traditional thermometers and their figures of merit, a concise description of optical thermometry methods, luminescence and instrumentation, and an explanation of the ways in which increases in temperature quench luminescence. Additional sections focus on materials utilized for luminescence thermometry and the broad range of applications for luminescence thermometry, including temperature measurement at the nanoscale and the application of multifunctional luminescent materials. Provides an overview of luminescence thermometry applications, including high-temperature, biomedical, nanoscale and multifunctional Delves into luminescence thermometry by materials group, including Rare-earth and transition Metal Ion Doped, Semiconductors, Quantum Dots and Organic materials Gives a concise introduction of the latest methods of temperature measurement, including luminescence spectroscopic schemes and methods of analysis

Download or read book Microfluidics and Nanofluidics Handbook written by Sushanta K. Mitra and published by CRC Press. This book was released on 2016-04-19 with total page 644 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive handbook presents fundamental aspects, fabrication techniques, introductory materials on microbiology and chemistry, measurement techniques, and applications of microfluidics and nanofluidics. The second volume focuses on topics related to experimental and numerical methods. It also covers fabrication and applications in a variety of areas, from aerospace to biological systems. Reflecting the inherent nature of microfluidics and nanofluidics, the book includes as much interdisciplinary knowledge as possible. It provides the fundamental science background for newcomers and advanced techniques and concepts for experienced researchers and professionals.

Download or read book Temperature Measurements in a Flow Using Laser Induced Fluorescence of Oxygen written by Franck Kolczak and published by . This book was released on 1992 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development of a Two color Laser induced Fluorescence Based Temperature Imaging Device with Micro scale Resolution written by Mark J. Gallina and published by . This book was released on 2002 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Development Steps of 2 color Laser induced Fluorescence with MDR enhanced Energy Transfer for Instantaneous Planar Temperature Measurement of Micro droplets and Sprays written by Johannes Palmer and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microfluidics and Nanofluidics Handbook 2 Volume Set written by Sushanta K. Mitra and published by CRC Press. This book was released on 2011-09-20 with total page 1767 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive, two-volume handbook on Microfluidics and Nanofluidics, this text covers fundamental aspects, fabrication techniques, introductory materials on microbiology and chemistry, measurement techniques, and applications with special emphasis on the energy sector. Each chapter begins with introductory coverage to a subject and then narrows in on advanced techniques and concepts, thus making it valuable to students and practitioners. The author pays special attention to applications of microfluidics in the energy sector and provides insight into the world of opportunities nanotechnology has to offer. Figures, tables, and equations to illustrate concepts.

Download or read book Handbook of Temperature Measurement Vol 2 written by Robin E. Bentley and published by Springer Science & Business Media. This book was released on 1998-09-01 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 2 of the Handbook of Temperature Measurement, prepared by the CSIRO National Measurement Laboratory, Australia, discusses the operation, calibration and usage of resistance and liquid-in-glass thermometers. Both standard-platinum-resistance thermometers and industrial-resistance thermometers are examined, and details on a variety of resistance-measuring techniques are given. Also included is a final version of the official text of the International Temperature Scale 1990 (ITS-90). The authors of this volume are John J. Connolly and E. Corina Horrigan.

Download or read book Microscale Optical Thermometry Techniques for Measuring Liquid Phase and Wall Surface Temperatures written by Myeongsub Kim and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermal management challenges for microelectronics are a major issue for future integrated circuits, thanks to the continued exponential growth in component density described by Moore's Law. Current projections from the International Technology Roadmap for Semiconductors predict that local heat fluxes will exceed 1 kW/cm2 within a decade. There is thus an urgent need to develop new compact, high heat flux forced-liquid and evaporative cooling technologies. Thermometry techniques that can measure temperature fields with micron-scale resolution without disturbing the flow of coolant would be valuable in developing and evaluating new thermal management technologies. Specifically, the ability to estimate local convective heat transfer coefficients, which are proportional to the difference between the bulk coolant and wall surface temperatures, would be useful in developing computationally efficient reduced-order models of thermal transport in microscale heat exchangers. The objective of this doctoral thesis is therefore to develop and evaluate non-intrusive optical thermometry techniques to measure wall surface and bulk liquid temperatures with O(1-10 micronmeter) spatial resolution. Intensity-based fluorescence thermometry (FT), where the temperature distribution of an aqueous fluorescent dye solution is estimated from variations in the fluorescent emission intensity, was used to measure temperatures in steady Poiseuille flow at Reynolds numbers less than 10. The flow was driven through 1 mm square channels heated on one side to create temperature gradients exceeding 8 °C/mm along both dimensions of the channel cross-section. In the evanescent-wave fluorescence thermometry (EFT) experiments, a solution of fluorescein was illuminated by evanescent waves to estimate the solution temperature within about 300 nm of the wall. In the dual-tracer FT (DFT) studies, a solution of two fluorophores with opposite temperature sensitivities was volumetrically illuminated over most of the `cross-section of the channel to determine solution temperatures in the bulk flow. The accuracy of both types of FT is determined by comparing the temperature data with numerical predictions obtained with commercial computational fluid dynamics software. The results indicate that EFT can measure wall surface temperatures with an average accuracy of about 0.3 °C at a spatial resolution of 10 micronmeter, and that DFT can measure bulk water temperature fields with an average accuracy of about 0.3 °C at a spatial resolution of 50 micronmeter in the image plane. The results also suggest that the spatial resolution of the DFT data along the optical axis (i.e., normal to the image plane) is at least an order of magnitude greater than the depth of focus of the imaging system.