Download or read book Mutants of the E Coli DnaA Gene written by Mark David Sutton and published by . This book was released on 1996 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Interaction of Escherichia Coli Gene Products with Mutant Forms of DnaA Protein written by Theodore Robert Hupp and published by . This book was released on 1990 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Gene Function written by Robert E. Glass and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: My aim in writing Gene Function has been to present an up-to-date picture ofthe molecular biology of Escherichia coli. I have not attempted a chronological description, believing that a mechanistic account is more useful for such a highly developed field. I have divided the book into four parts. Part I is a general introduction to bacterial systems, their genetic material, structure, composition and growth. It has seemed desirable to include herein a brief preview of the remaining text, to introduce the nomenclature and to help place subsequent chapters in perspective. The expression of genetic material and its perturbation through mutation is considered in Part II. Part III discusses how the transfer of prokaryotic genetic material can be mediated by plasmids and bacteriophages. It describes the DNA transactions involved (replication, recombination and repair) and ends with a description of the genetic and biochemical techniques employed in the study of gene organisation. Finally, Part IV considers the control of expression of bacterial, plasmid and phage genes. Key reviews are listed at the end of each chapter.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Escherichia coli DNA replication mutant with a point mutation in a transfer RNA gene written by Ken-Shiung Chen and published by . This book was released on 1989 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Life Sciences written by Robert D. Wells and published by Springer. This book was released on 2018-01-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Life Sciences: An Encyclopedic Reference will focus on understanding biological phenomena at the level of molecules and their interactions that govern life processes. The work will include articles on genes and genomes, protein structure and function, systems biology using genomics and proteomics as the focus, molecular aspects of cell structure and function, unifying concepts and theories from biology, chemistry, mathematics and physics that are essential for understanding the molecular life sciences (including teaching perspectives and assessment tools), and basic aspects of the various experimental approaches that are used in the Molecular Life Sciences.
Download or read book Growth Rate Regulation and Control of Initiation of DNA Replication in Escherichia Coli written by Anne Eliane Chiaramello and published by . This book was released on 1990 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.
Download or read book Immunology and Evolution of Infectious Disease written by Steven A. Frank and published by Princeton University Press. This book was released on 2002-07-21 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description
Download or read book DNA Repair and Mutagenesis written by Errol C. Friedberg and published by American Society for Microbiology Press. This book was released on 2005-11-22 with total page 2587 pages. Available in PDF, EPUB and Kindle. Book excerpt: An essential resource for all scientists researching cellular responses to DNA damage. • Introduces important new material reflective of the major changes and developments that have occurred in the field over the last decade. • Discussed the field within a strong historical framework, and all aspects of biological responses to DNA damage are detailed. • Provides information on covering sources and consequences of DNA damage; correcting altered bases in DNA: DNA repair; DNA damage tolerance and mutagenesis; regulatory responses to DNA damage in eukaryotes; and disease states associated with defective biological responses to DNA damage.
Download or read book Investigation of Binding Features Between E coli DnaA Mutant Proteins and DNA written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods for the Identification and Study of Required Genes in Escherichia Coli written by Roger Allyn Forsyth and published by . This book was released on 1999 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Required genes in Escherichia coli are those products which are essential for sustained growth and division. This dissertation describes the use of conditionally expressed antisense RNA to probe for genes required for growth under laboratory conditions. E. coli chromosomal fragments were cloned behind an IPTG inducible promoter. Out of 66,000 clones, 11 clones with inserts of 1 KB or less were sequenced, six of which produced RNA antisense to a portion of a chromosomally encoded mRNA. The antisense RNA's are complementary to mRNA's coding for ddlB (involved in peptidoglycan precursor synthesis), lepB (leader peptidase), and two previously unamed orfs. One orf, viaA (viability inhibited by antisense) was further characterized. ViaA is located in the periplasm and is a member of the thioredoxin family. Several interesting phenotypes associated with overexpression and loss of activity of ViaA are described. Another essential gene dnaA, required for initiating DNA replication, was studied using either a knockout with conditional plasmid complementation or inducible antisense. The first system verified that an excess of DnaA will increase the rate of chromosomal initiation events but that initiation events halt upon protein synthesis inhibition. DnaA overproduction also causes cell filamentation but not via an SOS mechanism. Regulation of dnaA was also studied using a dnaA-chb fusion integrated into the chromosome. A point mutation in the GUG start codon of dnaA to AUG revealed that the start codon utilization regulates the ultimate level of DnaA in the cell. Translation from both codons is correlated with growth rate although translation from the wildtype GUG start codon is lower than from the AUG mutated codon at all growth rates.
Download or read book Effects of Isolated Missense Mutations from the DnaA5 and DnaA46 Alleles in Initiation of Escherichia Coli OriC Replication written by Kevin Michael Carr and published by . This book was released on 1994 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Mutagens written by and published by . This book was released on 1971 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of the DnaA Promoter in Escherichia Coli written by Victoria Goehner Newman and published by . This book was released on 1999 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: The timing of initiation of DNA replication from oriC in E. coli is regulated by the concentration of the DnaA protein. Increases in the intracellular DnaA protein concentration over 1.5 fold normal levels causes asynchronous timing of initiation of DNA replication. The regulators of the dnaA operon must keep the DnaA concentration within tight limits yet be able to respond to rapidly changing environmental conditions. Here it is shown that Fis and DnaA proteins interact, in conjunction with other factors, to regulate the expression of dnaA. Binding of DnaA to the DnaA box in the dnaA promoter region is destabilized by the binding of Fis to its binding site in dnaAp2. However, DnaA94, containing only the C-terminal 94 amino acids of the DnaA protein and retaining specific binding activity to the DnaA box of the dnaA promoter region, is not destabilized by Fis binding. DNase I and copper-phenanthroline (Cu-op) footprinting studies of DnaA and DnaA94 support the existence of a second weak DnaA box which abuts the DNase I and Cu-op footprints of Fis in the dnaAp2 promoter. We determined that the GC-rich region of the dnaAp2 promoter is analogous to the discriminator sequence (DS) of rRNA promoters, in that the GC-rich sequence mediates the repression caused by elevated levels of guanosine tetraphosphate (ppGpp). During starvation conditions, the alarmone ppGpp is synthesized. Overexpression of ppGpp causes inhibition of dnaAp2 promoter transcription, which is alleviated when the GC-rich DS is mutated to an AT-rich sequence. We conclude that this site is responsible for mediating the stringent control of the dnaAp2 promoter. Incorrect timing of initiation in the cell cycle results in asynchronous replication. Strains with a mutated DS, DnaA box, or Fis binding site in the dnaA promoter region on the chromosome have increased levels of dnaA gene expression. These mutants initiate DNA replication synchronously in minimal M9 medium as shown by flow cytometric assays. In rich medium, the DS, mutant showed very limited asynchrony, whereas the other mutants replicated synchronously.
Download or read book Novel DnaA Alleles of Escherichia Coli that are Hyperactive in Initiation of Chromosomal DNA Replication written by Alec Jude Murillo and published by . This book was released on 2008 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Recombinational Repair of DNA Damage written by Andrei Kuzminov and published by Landes Bioscience. This book was released on 1996 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Short Course in Bacterial Genetics written by Jeffrey H. Miller and published by CSHL Press. This book was released on 1992 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: University of California, Los Angeles. Introduction to bacterial genetics, including laboratory methods, for advanced students and beginning researchers. Handbook with plastic spiral-bound laboratory manual.
