Download or read book Molecular Methods for Virus Detection written by Danny L. Wiedbrauk and published by Elsevier. This book was released on 1995-02-08 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables

Download or read book Molecular Virology of Human Pathogenic Viruses written by Wang-Shick Ryu and published by Academic Press. This book was released on 2016-03-30 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Virology of Human Pathogenic Viruses presents robust coverage of the key principles of molecular virology while emphasizing virus family structure and providing key context points for topical advances in the field. The book is organized in a logical manner to aid in student discoverability and comprehension and is based on the author's more than 20 years of teaching experience. Each chapter will describe the viral life cycle covering the order of classification, virion and genome structure, viral proteins, life cycle, and the effect on host and an emphasis on virus-host interaction is conveyed throughout the text. Molecular Virology of Human Pathogenic Viruses provides essential information for students and professionals in virology, molecular biology, microbiology, infectious disease, and immunology and contains outstanding features such as study questions and recommended journal articles with perspectives at the end of each chapter to assist students with scientific inquiries and in reading primary literature. - Presents viruses within their family structure - Contains recommended journal articles with perspectives to put primary literature in context - Includes integrated recommended reading references within each chapter - Provides access to online ancillary package inclusive of annotated PowerPoint images, instructor's manual, study guide, and test bank

Download or read book Molecular Methods in Plant Disease Diagnostics written by Neil Boonham and published by CABI. This book was released on 2016-04-19 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using molecular methods for plant disease diagnosis provides diagnosticians with a number of advantages over more traditional methods. They can allow the identification of morphologically similar species, for example, or the detection of infection prior to symptom formation. Not only can molecular tools help by increasing the efficacy, accuracy and speed of diagnosis; their common technological basis provides further benefits, especially where resources are limited and traditional skills are hard to sustain. This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.

Download or read book Molecular Virology written by Susanne Modrow and published by Springer. This book was released on 2013-09-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.

Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.

Download or read book Molecular Detection of Animal Viral Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2016-05-25 with total page 952 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Detection of Animal Viral Pathogens presents expert summaries on state-of-the-art diagnostic approaches for major animal viral pathogens, with a particular emphasis on identification and differentiation at the molecular level. Written by specialists in related research areas, each chapter provides a concise overview of an individual virus

Download or read book Molecular Detection of Human Viral Pathogens written by Dongyou Liu and published by CRC Press. This book was released on 2016-04-19 with total page 1152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite being recognized and fought against over countless centuries, human viral pathogens continue to cause major public health problems worldwide-killing millions of people and costing billions of dollars in medical care and lost productivity each year. With contributions from specialists in their respective areas of viral pathogen research, Mol

Download or read book Manual of Commercial Methods in Clinical Microbiology written by and published by John Wiley & Sons. This book was released on 2016-03-28 with total page 652 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Manual of Commercial Methods in Clinical Microbiology 2nd Edition, International Edition reviews in detail the current state of the art in each of the disciplines of clinical microbiology, and reviews the sensitivities, specificities and predictive values, and subsequently the effectiveness, of commercially available methods – both manual and automated. This text allows the user to easily summarize the available methods in any particular field, or for a specific pathogen – for example, what to use for an Influenza test, a Legionella test, or what instrument to use for identification or for an antibiotic susceptibility test. The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition presents a wealth of relevant information to clinical pathologists, directors and supervisors of clinical microbiology, infectious disease physicians, point-of-care laboratories, professionals using industrial applications of diagnostic microbiology and other healthcare providers. The content will allow professionals to analyze all commercially available methods to determine which works best in their particular laboratory, hospital, clinic, or setting. Updated to appeal to an international audience, The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition is an invaluable reference to those in the health science and medical fields.

Download or read book Testing Methods for Seed transmitted Viruses written by S. E. Albrechtsen and published by CABI. This book was released on 2006 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

Download or read book DNA Viruses written by Paul M. Lieberman and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.

Download or read book Diagnostic Virology Protocols written by John R. Stephenson and published by Springer Science & Business Media. This book was released on 1998-08-28 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

Download or read book Basic and Applied Aspects of Biotechnology written by Varsha Gupta and published by Springer. This book was released on 2016-10-22 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.

Download or read book Molecular Diagnostics of Infectious Diseases written by Harald H. Kessler and published by Walter de Gruyter GmbH & Co KG. This book was released on 2014-07-28 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: The prevalence of infectious diseases is worldwide increasing. Therefore, detection methods for infectious pathogens change quickly. In the 3rd edition of Kessler ́s Molecular Diagnostics of Infectious Diseases laboratory professionalists get valueable information about the current diagnostic methods, tipps and tricks in terms of sample processing, quality control, and interpretation of the results. For clinicians the book is a valuable aid for decision-making in ordering appropriate tests as well as in assuring the necessary quality of the sample material.

Download or read book Developing Norms for the Provision of Biological Laboratories in Low Resource Contexts written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2019-05-01 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: On June 27-28, 2018, the U.S. National Academies of Sciences, Engineering, and Medicine (the National Academies) convened an international workshop in Amsterdam, the Netherlands, on developing norms for the provision of laboratories in low-resource contexts. The U.S. Department of State's Biosecurity Engagement Program requested that the National Academies organize this workshop to engage an international group of organizations that provide funding for construction, upgrades, and maintenance of biological laboratories in countries without the means to build such labs themselves. Twenty-one people from 19 organizations participated. The intent was to advance the conversation about the identification and application of guiding principles and common norms for use by these organizations in their grants, partnerships, and aid. This publication summarizes the presentations and discussions from the workshop.

Download or read book Biotechnology and Plant Disease Management written by Zamir K. Punja and published by CABI. This book was released on 2007-01-01 with total page 591 pages. Available in PDF, EPUB and Kindle. Book excerpt: As agricultural production increases to meet the demands of a growing world population, so has the pace of biotechnology research to combat plant disease. Diseases can be caused by a variety of complex plant pathogens including fungi, bacteria, viruses and nematodes, and their management requires the use of techniques in transgenic technology, biochemistry and genetics. While texts exist on specific pathogens or management practices, a comprehensive review is needed of recent developments in modern techniques and the understanding of how pathogens cause disease. This collection of studies discusses the key approaches to managing each group of pathogens within the context of recent developments in biotechnology. Broad themes include microbe-plant interactions, molecular diagnostics of plant pathogens and enhancing the resistance of plants.

Download or read book Development of Molecular Methods to Detect Infectious Viruses in Water written by T. Cromeans and published by IWA Publishing. This book was released on 2005-01-01 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enteric viruses have been detected in environmental waters and potable water supplies. Water supply safety is evaluated by examination for bacteria that are not accurate surrogates for viruses that pose a public health threat in low levels. Current viral detection methods include standard techniques for in vitro cultivation in cell cultures and newer molecular methods for viral nucleic acid detection. The new methods are rapid and sensitive, but detect only the viral nucleic acid and do not determine infectivity. Methods for the detection of potentially infectious viruses are needed. The objectives of this project were todevelop and evaluate sensitive, selective methods for infectious virus capture from water concentrates; develop and evaluate cell culture combined with rapid, sensitive RT-PCR and PCR methods for detection of infectious viruses; developmethods for rapid, sensitive, and specific quantitation of viruses; validate the performance of methods for water sample concentrates containing assay inhibitory substances; validate the ability of methods to distinguish infectious from non-infectious viruses inactivated by disinfectants with different inactivation mechanisms, free chlorine, and low pressure UV radiation; and collect, concentrate, and archive raw and finished water samples for subsequent virus analysis.New molecular tests such as RT-PCR or PCR may yield ambiguous positive results by detecting non-infectious or inactivated viruses. To overcome this problem, this study employed additional processing steps that depend on functional components of the virus needed for infection as an approach to detecting only infectious viruses. These methods were intended to preclude the detection of non-infectious viruses and increase the potential for detecting infectious viruses. Methods included the specific capture of virus by cellular receptors for virus in vitro, followed by molecular detection of viral nucleic acid in the captured virus. Another method demonstrated was the selection and detection of specific nucleic acid only present in the cell during virus replication. In addition, rapid real-time molecular tests that may be integrated with methods for the functional components were developed. The following are highlights from the project:Methods for detection of ribonucleic acid forms only present in inoculated cells after viral infection were developed and validated for two different types of viruses potentially contaminating water sources—enteroviruses and Adenoviruses. A purified cellular protein necessary for attachment of Coxsackie B viruses to cells was used to capture potentially infectious viruses for detection by RT-PCR. Real-time RT-PCR and PCR assays, complete in 30–90 minutes, were developed for enterovirus, hepatitis A virus, Adenovirus, and Norovirus detection. These assays can be used in conjunction with some of the other developed methods to detect infectious virus.The increasing need for the reuse of water and the inadvertent reuse of natural sources of water impacted by point and non-point human waste discharges make detection of infectious viruses in source and finished water supplies an important need. The researchers have developed and demonstrated several new methods for the selective detection of potentially infectious, pathogenic viruses transmitted by water. Rapid real-time molecular detection methods were developed that can be combined with the infectivity assays for rapid detection of infectious viruses. Further work can expand these methods to other viruses. These assays can be used in the future at utilities when new technology is commercially available for discrimination of infectious and non-infectious viruses.Originally published by AwwaRF for its subscribers in 2004. This publication can also be purchased and downloaded via Pay Per View on Water Intelligence Online - click on the Pay Per View icon below

Download or read book Sustainable disease management in a European context written by David B. Collinge and published by Springer Science & Business Media. This book was released on 2008-09-02 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: The main theme of the book is sustainable disease management in a European context. Some of the questions addressed are: How does society benefit from plant pathology research? How can new molecular approaches solve relevant problems in disease management? What other fields can we exploit in plant pathology research? What challenges are associated with free trade across the new borders? How can we contribute to solving problems of developing countries? How does plant pathology contribute to food quality and safety? How does globalization/internationalization affect teaching and extension in plant pathology?