Download or read book Molecular Marker Analysis of Inbreeding in Alfalfa Medicago Stavi L written by Douglas James Brouwer and published by . This book was released on 1995 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Insights Into the Genome of Alfalfa Medicago Sativa L written by Ian M. Ray and published by . This book was released on 1989 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Inbreeding Effects on Diploid tetraploid Genetic Alfalfa M Sativa L and M Falcata L Counterparts written by Oscar Rafael De Cordova and published by . This book was released on 1973 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Inbreeding Depression as a Measure of Improvement in Alfalfa Medicago Sativa L written by Mohamed A. El-Nahrawy and published by . This book was released on 1987 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Comparison of the Effects of Inbreeding on Isogenic Diploid tetraploid Alfalfa Medicago Sativa L written by Cheryl C. Fox and published by . This book was released on 1981 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Analysis of Complex Traits in Alfalfa Medicago Sativa L written by Joseph Gary Robins and published by . This book was released on 2004 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genetic structure of complex agronomic traits in alfalfa (Medicago sativa) is not well understood. By crossing the subspecies M. sativa subsp. falcata and M. sativa subsp. sativa, a fullsib F1 population was created from which a genetic linkage map of each parental genome was developed using RFLP and SSR markers. These maps include simplex, duplex, and simplex-simplex alleles along with a number of alleles exhibiting segregation distortion. The inclusion of these more complicated segregation ratios resulted in greater saturation of the genome, a better convergence to eight consensus linkage groups, and a more realistic view of regions of the genome that may not behave normally due to segregation distortion than would have been possible by only using simplex alleles as has been done previously. The population was clonally propagated and grown at three field locations with phenotypic data collected over three years for various agronomic traits, including biomass production, forage height, and forage regrowth. Combining the marker data with the phenotypic data, markers were identified from each parental genome that were associated with these traits, suggesting that both major germplasm sources of cultivated alfalfa contain alleles that may contribute to improved alfalfa cultivars. These results provide a much better understanding of the genomic regions underlying these traits and are an important start in efforts aimed at the use of marker-assisted selection for the improvement of alfalfa cultivars.
Download or read book Inbreeding Depression in Three Populations of Autotetraploid Alfalfa Medicago Sativa L written by Jennifer Sue Jones and published by . This book was released on 1993 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Inbreeding Alfalfa Medicago Sativa L by Sib mating written by Marvin Kenneth Aycock and published by . This book was released on 1966 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Understanding Complex Traits in Alfalfa Through Transcriptomics Genomics and Proteomics written by Atit Parajuli and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Alfalfa (Medicago sativa L.) is a perennial, outcrossing legume crop predominantly grown for hay, silage, or pasture. Genetic improvement in Alfalfa in terms of hay yield is still comparable to 30 years ago. Under a variety of growing conditions, forage yield in Alfalfa is stymied by biotic and abiotic stresses including heat, salt, drought, and disease. To overcome such stresses, Alfalfa uses a differential gene expression pathway which is under the control of transcription factors that contribute to tolerance of stresses. The Alfalfa breeding program is mainly focused on developing synthetic varieties through recurrent phenotypic selection exploiting additive genetic effects. The production of hybrid Alfalfa breeding programs uses synthetic varieties as the most feasible means for genetic gain. High heterozygosity of the plants and severe inbreeding depression upon selfing precludes the development of inbred lines for hybrid production. However, quantifying inbreeding depression through fitness and vigor traits expressed as weak and strong plants can help map these traits using association study. Identifying these genetic variants paves the way for the elimination of deleterious alleles and eventually the development of inbred alfalfa lines for hybrid production. However, genetic regions identified through association study do not always translate to actual functional proteins as they are not always linked to genes or genetic variants responsible for traits of interest. As the protein's biological function is strongly dependent on its 3D structure, associating proteins directly with phenotype could help assess the effect of mutation on protein function. To understand the role of transcription factors in stress tolerance, we identified and performed transcriptome analysis of Basic-leucine zipper (bZIP) transcription factors that have played a critical role in regulating growth and development and mediating the responses to abiotic stress in several species, including Arabidopsis thaliana, Oryza sativa, Lotus japonicus, and Medicago truncatula. We identified 237 bZIP genes that were differentially expressed in response to ABA, cold, drought, and salt stresses, indicating a likely role in abiotic stress signaling and/or tolerance. These expressions were further validated through RT-qPCR analysis. Next, a genome-wide association study was performed to map genetic loci associated with Alfalfa for plant vigor trait using 534 plants collected from three locations (Washington, Wisconsin, and Utah) over three generations of selfing. These plants were selected based on plant health of strong and weak within the same line. A total of 11 genetic loci were identified using 588,136 Single nucleotide polymorphisms (SNPs). Gene ontology analysis of significant loci associated them with genes involved in stress response, defense responses against pathogens, and plant reproduction. Finally, we attempted the first-ever association study between features from alphafold predicted 3D structure of protein and phenotype, to link non-synonymous mutation to phenotypes. We used 154 genes, including significant genes from the GWAS study, after filtering 591,919 SNPs, to predict protein 3D structures that identified the five significant GWAS hits. However, two more genes with the lowest p-values (Nod 19, Cytochrome P450) were also identified which play key roles in plant growth and development and also in stress tolerance. This association study is a promising way to narrow down causal mutations from SNP GWAS through stringent filtering of SNPs.
Download or read book Inbreeding Alfalfa Medicago Sativa L by Selfing Sib mating and Backcrossing written by Gerry Lynn Posler and published by . This book was released on 1969 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Inbreeding Behavior in Selected Clones of Alfalfa Medicago Sativa L written by Mohammad Mokhlesur Rahman and published by . This book was released on 1968 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Genetic Study of the Multifoliolate Characteristic in Alfalfa Medicago Sativa L written by Mark Anthony Brick and published by . This book was released on 1975 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: