Download or read book Molecular Cytogenetics of Fishes written by Marcelo B. Cioffi and published by S. Karger AG (Switzerland). This book was released on 2013 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fish exhibit the greatest diversity of all vertebrates, making this group extremely attractive for the study of a number of evolutionary questions. Besides, fish genomes have intrinsic characteristics that may be responsible for this amazing diversity, but little is known about their structure and organization. Major advances in chromosomal studies were achieved in the current 'molecular cytogenetic era' based on detecting DNA sequences on chromosomes, parts of chromosomes or even whole genomic DNA by fluorescence in situ hybridization. In this issue, a collection of review articles is presented together with current research results, demonstrating the advances made using this specific methodology in fish research. The contributions provide a timely update on recent developments in fish molecular cytogenetics, covering topics such as karyotype evolution, sex and B chromosomes, polyploids, and molecular evolution. Researchers, teachers, and students interested in the structural features and evolutionary dynamics of fish genomes will find this publication a valuable source of current knowledge.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer Science & Business Media. This book was released on 2008-11-26 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Molecular Cytogenetics written by Yao-Shan Fan and published by Springer Science & Business Media. This book was released on 2002-09-13 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
Download or read book Fish Cytogenetics written by E Pisano and published by CRC Press. This book was released on 2007-01-03 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past 20 years, fish cytogenetics has become an essential tool in fields as diverse as systematics and evolution, conservation, aquaculture and more recently, genomics. This book is organized in four sections (systematics and evolution; biodiversity conservation; stock assessment and aquaculture; genomics) covering the major fields of present
Download or read book Fish Cytogenetic Techniques written by Catherine Ozouf-Costaz and published by CRC Press. This book was released on 2015-06-17 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in fish cytogenetics have enhanced the interest in chromosome analysis in both fundamental (systematics and comparative genomics among fishes and other vertebrate groups) and applied (aquaculture, conservation and response to pollutants, whole genome sequencing of model fish species) research. Although the genomic material, the chro
Download or read book Cytogenetics FISH and Molecular Testing in Hematologic Malignancies written by Wojciech Gorczyca and published by CRC Press. This book was released on 2008-08-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics, fluorescence in situ hybridization (FISH) and molecular tests, especially polymerase chain reaction (PCR), play an important role in the management of patients with hematologic malignancies by helping to establish the diagnosis, as well as predict prognosis, response to treatment and disease progression. Chromosomal and molecular abnormalities provide the most reliable criteria for classification of hematopoietic tumors and often comprise the basis for targeted therapy. Cytogenetics, FISH and Molecular Testing in Hematologic Malignancies, provides a review of chromosomal and molecular changes in hematologic malignancies and correlates the karyotypic and genetic abnormalities with morphology, immunophenotype and clinical data. With over 180 figures and diagnostic algorithms, this text is essential reading for all pathologists, hematopathologists, hematologic oncologists, cytogenetists, cytogenetic technologists and cell biologists.
Download or read book Fish Karyotypes written by Ryoichi Arai and published by Springer Science & Business Media. This book was released on 2011-06-26 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the largest group of extant vertebrates, fish offer an almost limitless number of striking examples of evolutionary adaptation to environmental and biotic selection pressure. The most diverse of all vertebrate groups, the higher taxa of fish traditionally have been classified by morphology and paleontology, with a much smaller input of cytogenetic information. DNA sequence data are exerting an increasingly strong influence on modern fish systematics, challenging the classification of numerous higher taxa ranging from genera to orders. The most fruitful approach, however, involves synthetic analyses of morphology, molecular phylogenetics, comparative karyology, and genome size. Karyotypes of more than 3400 species/subspecies are arranged here by fish systematics and include a list of genome size, sex chromosomes, B chromosomes, polyploidy, and locality of material fish, among others. This volume enables both beginners and advanced researchers to survey the existing literature and facilitates the implementation of an integrative approach to fish systematics. The first book on fish chromosomes in nearly 15 years, it is also the most comprehensive.
Download or read book Molecular Cytogenetics written by Yao-Shan Fan and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 409 pages. Available in PDF, EPUB and Kindle. Book excerpt: The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.
Download or read book Cytogenetics written by Marcelo L. Larramendy and published by BoD – Books on Demand. This book was released on 2019-05-10 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics - Past, Present, and Further Perspectives discusses events that influenced the development of cytogenetics as a specialty within biology, with special attention paid to methodological achievements developed worldwide that have driven the field forward. Improvements to the resolution of chromosome analysis followed closely the introduction of innovative analytical technologies. In that sense, this book reviews and provides a brief account of the structure of chromosomes and stresses the high structural conservation in different species with an emphasis on aspects that require further research. However, it should be kept in mind that the future of cytogenetics will likely depend on improved knowledge of chromosome structure and function.
Download or read book Molecular Genetics in Fisheries written by Gary R. Carvalho and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basic principle of all molecular genetic methods is to employ inherited, discrete and stable markers to identify genotypes that characterize individuals, populations or species. Such genetic data can provide information ori the levels and distribution of genetic variability in relation to mating patterns, life history, population size, migration and environment. Although molecular tools have long been employed to address various questions in fisheries biology and management, their contributions to the field are sometimes unclear, and often controversial. Much of the initial impetus for the deployment of molecular markers arose from the desire to assess fish stock structure based on various interpretations of the stock concept. Although such studies have met with varying success, they continue to provide an impetus for the development of increasingly sensitive population discriminators, yielding information that can be valuable for both sustainable exploitation and the conservation of fish populations. In the last major synthesis of the subject, Ryman and Utter (1987) summarized progress and applications, though this was prior to the wide-scale adoption of DNA methodology. New sources of genetic markers and protocols are now available, in particular those that exploit the widely distributed and highly variable repeat sequences of DNA, and the amplification technique of the polymerase chain reaction.
Download or read book Fluorescence In Situ Hybridization FISH Application Guide written by Thomas Liehr and published by Springer. This book was released on 2008-11-18 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.
Download or read book Marine Genetics written by Antonio M. Solé-Cava and published by Springer Science & Business Media. This book was released on 2000-05-31 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: International Workshop on Marine Genetics - Rio 98
Download or read book Molecular Cytogenetics written by Yao-Shan Fan and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
Download or read book Cytogenetic Laboratory Management written by Susan Mahler Zneimer and published by John Wiley & Sons. This book was released on 2016-11-21 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetic Laboratory Management Cytogenetic Laboratory Management Chromosomal, FISH and Microarray-Based Best Practices and Procedures Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures is a practical guide that describes how to develop and implement best practice processes and procedures in the genetic laboratory setting. The text first describes good laboratory practices, including quality management, design control of tests, and FDA guidelines for laboratory-developed tests, and preclinical validation study designs. The second focus of the book is on best practices for staffing and training, including cost of testing, staffing requirements, process improvement using Six Sigma techniques, training and competency guidelines, and complete training programs for cytogenetic and molecular genetic technologists. The third part of the text provides stepwise standard operating procedures for chromosomal, FISH and microarray-based tests, including preanalytic, analytic, and postanalytic steps in testing, which are divided into categories by specimen type and test type. All three sections of the book include example worksheets, procedures, and other illustrative examples that can be downloaded from the Wiley website to be used directly without having to develop prototypes in your laboratory. Providing a wealth of information on both laboratory management and molecular and cytogenetic testing, Cytogenetic Laboratory Management will be an essential tool for laboratorians worldwide in the field of laboratory testing and genetic testing in particular. This book gives the essentials of: Developing and implementing good quality management programs in laboratories Understanding design control of tests and preclinical validation studies and reports FDA guidelines for laboratory-developed tests Use of reagents, instruments, and equipment Cost of testing assessment and process improvement using Six Sigma methodology Staffing training and competency objectives Complete training programs for molecular and cytogenetic technologists Standard operating procedures for all components of chromosomal analysis, FISH, and microarray testing of different specimen types This volume is a companion to Cytogenetic Abnormalities: Chromosomal, FISH and Microarray-Based Clinical Reporting. The combined volumes give an expansive approach to performing, reporting, and interpreting cytogenetic laboratory testing and the necessary management practices, staff and testing requirements.
Download or read book Genetics Evolution and Conservation of Neotropical Fishes written by Rodrigo A. Torres and published by Frontiers Media SA. This book was released on 2020-01-21 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fish represent the most ancestral and specious group of vertebrates, and occupy more diverse aquatic environments around the world. Ichthyofauna is extremely diverse, especially in megadiverse countries occupying biogeographical regions such as the Neotropical Region, which covers an extensive area between North and South America. Much of this biodiversity will be extinct, even before science knows any aspect of its biology. Like this, Neotropical fish genetics started in the end of the 70’s with papers studying the chromosomes of Hoplias malabaricus (Family Erythrinidae) and the karyotype variation among three genera of the family Anostomidae. The topic at that time was concentrated in two Institutions from the state of São Paulo, Southeastern Brazil. In the middle 80’s, the first Symposium on Neotropical Fish Cytogenetics was organized. Nowadays, the field of Neotropical Fish Genetics is present in Brazil, Colombia, Argentina, Uruguai, Venezula, Chile, and Equador, as well as outside South America in Panama, Mexico, USA, Canada, Czech Republic, Germany, and Spain. The research developed in cytogenetics has focused mainly on karyotype evolution and cytotaxonomy, chromosome structure and, more recently, cytogenomics. In relation to the use of molecular markers, support has been sought for the management of populations for conservation or production in captivity. In addition, many studies have been carried out with the aim of establishing supra-specific phylogenetic relationships and clarifying species distribution scenarios by phylogeographic modeling. The genome and transcriptome of some model species begin to emerge as extremely promising and informative areas for neotropical fish. In 2017, the Neotropical fish genetics research community celebrates the 30th anniversary of its main Meeting (today entitled Symposium on Neotropical Fish Genetics and Cytogenetics). This Research Topic is part of this celebration and aims at reporting the state of the art and its current advances in the frontier of knowledge in genetics, evolution, and conservation of neotropical fish, as well as to detect the challenges to be overcome in the next years.
Download or read book The AGT Cytogenetics Laboratory Manual written by Marilyn S. Arsham and published by John Wiley & Sons. This book was released on 2017-04-24 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytogenetics is the study of chromosome morphology, structure, pathology, function, and behavior. The field has evolved to embrace molecular cytogenetic changes, now termed cytogenomics. Cytogeneticists utilize an assortment of procedures to investigate the full complement of chromosomes and/or a targeted region within a specific chromosome in metaphase or interphase. Tools include routine analysis of G-banded chromosomes, specialized stains that address specific chromosomal structures, and molecular probes, such as fluorescence in situ hybridization (FISH) and chromosome microarray analysis, which employ a variety of methods to highlight a region as small as a single, specific genetic sequence under investigation. The AGT Cytogenetics Laboratory Manual, Fourth Edition offers a comprehensive description of the diagnostic tests offered by the clinical laboratory and explains the science behind them. One of the most valuable assets is its rich compilation of laboratory-tested protocols currently being used in leading laboratories, along with practical advice for nearly every area of interest to cytogeneticists. In addition to covering essential topics that have been the backbone of cytogenetics for over 60 years, such as the basic components of a cell, use of a microscope, human tissue processing for cytogenetic analysis (prenatal, constitutional, and neoplastic), laboratory safety, and the mechanisms behind chromosome rearrangement and aneuploidy, this edition introduces new and expanded chapters by experts in the field. Some of these new topics include a unique collection of chromosome heteromorphisms; clinical examples of genomic imprinting; an example-driven overview of chromosomal microarray; mathematics specifically geared for the cytogeneticist; usage of ISCN’s cytogenetic language to describe chromosome changes; tips for laboratory management; examples of laboratory information systems; a collection of internet and library resources; and a special chapter on animal chromosomes for the research and zoo cytogeneticist. The range of topics is thus broad yet comprehensive, offering the student a resource that teaches the procedures performed in the cytogenetics laboratory environment, and the laboratory professional with a peer-reviewed reference that explores the basis of each of these procedures. This makes it a useful resource for researchers, clinicians, and lab professionals, as well as students in a university or medical school setting.
Download or read book Molecular Systematics of Fishes written by Thomas D. Kocher and published by Elsevier. This book was released on 1997-07-10 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequenced biological macromolecules have revitalized systematic studies of evolutionary history. Molecular Systematics of Fishes is the first authoritative overview of the theory and application of these sequencing data to fishes. This volume explores the phylogeny of fishes at multiple taxonomic levels, uses methods of analysis of molecular data that apply both within and between fish populations, and employs molecule-based phylogenies to address broader questions of evolution. Targeted readers include ichthyologists, marine scientists, and all students, faculty, and researchers interested in fish evolution and ecology and vertebrate systematics. - Focuses on the phylogeny and evolutionary biology of fishes - Contains phylogenies of fishes at multiple taxonomic levels - Applies molecule-based phylogenies to broader questions of evolution - Includes methods for critique of analysis of molecular data
