Download or read book Molecular Characterization of a Rhoptry Antigen of Plasmodium Falciparum written by Heidi Juliette Brown and published by . This book was released on 1991 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Characterization of the Ornithine Decarboxylase Gene of the Human Malaria Parasite Plasmodium Falciparum written by Lyn-Marié Birkholtz and published by . This book was released on 1998 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Characterization of Membrane Associated Inner Membrane Complex Proteins in Plasmodium Falciparum Welch 1897 written by Dipto Sinha and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular Characterization of Plasmodium Falciparum Infection and Development of the Humoral Immune Response in Persistently Infected Ghanaian Infants written by Simon Richard Franks and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Vesicle Targeting in Plasmodium Falciparum written by Lawrence Sumanjah Ayong and published by . This book was released on 2009 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins of the SNARE (Soluble N-ethylmaleimide sensitive factor attachment protein receptor) super-family have been characterized as playing an essential role in vesicle targeting and fusion in all eukaryotes. The intracellular malaria parasite Plasmodium falciparum exhibits an unusual endomembrane system that is characterized by an unstacked Golgi apparatus, a developmentally induced apical complex, and various organellar structures of parasite origin in the infected host cells. How malaria parasites target nuclear-encoded proteins to these novel compartments is a central question in Plasmodium cell biology. Ultrastructural studies elsewhere have implicated the participation of specialized vesicular elements in transport of virulence proteins, including various cytoadherance and host cell remodeling factors, into the infected erythrocyte cytoplasm. However, little is known about the machineries that define the directionality of vesicle trafficking in malaria parasites. We hypothesized that the P. falciparum SNARE proteins would exhibit novel features required for vesicle targeting to the parasite-specific compartments. We then identified for the first time and confirmed the expression of eighteen SNARE genes in P. falciparum. Members of the PfSNAREs exhibit atypical structural features (Ayong et al., 2007, Molecular & Biochemical Parasitology, 152(2), 113-122). Among the atypical PfSNAREs, PfSec22 contains an unusual insertion of the Plasmodium export element (PEXEL) within its profilin-like longin domain, preceded by an N-terminal hydrophobic segment.
Download or read book Molecular Characterization of the Hexose Transporter PfHT1 of Plasmodium Falciparum in Xenopus Laevis Oocytes written by Suzanne Kathryn Manning and published by . This book was released on 2001 with total page 129 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification Characterization and Amino terminal Sequencing of High Molecular Weight Soluble Antigens of the Human Malaria Parasite Plasmodium Falciparum written by James Harold Nichols and published by . This book was released on 1990 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plasmodium falciparum is the most lethal of four species of protozoa that cause malaria in humans. Since the blood stage of the parasite life cycle is responsible for the clinical manifestations of the disease, studies of the immune response and vaccine development to this stage are critical to decreasing the morbidity and mortality of malaria. Immunization of experimental monkeys with crude supernatant fractions obtained from in vitro culture media or partially purified fractions of culture media have been shown to protect them from malaria. Protective immunity is known to occur naturally in afflicted adolescent and adult individuals only after long-term exposure to malaria. This research focuses on soluble antigens, termed exoantigens, secreted into the supernatant of in vitro blood stage cultures. These exoantigens are believed to either be shed or secreted from the parasite surface during red blood cell invasion. The goal of this research was to identify and characterize those exoantigens capable of eliciting an antibody response which could act as potential vaccine target antigens. Crude supernatant from normal uninfected red blood cell lysates and human sera, or from cultures infected with the Vietnamese (Indochina I) strain of Plasmodium falciparum were fractionated in parallel by HPLC gel filtration and DEAE ion exchange chromatography. Western blots were performed on the infected and control fractions using an African human sera pool obtained from adult individuals with confirmed long-term exposure to Plasmodium falciparum. Sixteen antigens were identified in the range of 100-250 Kd under reducing SDS conditions. These exoantigens were further reactive against pools of sera from Malaysian and Venezuelan adults with long-term exposure. The reactive to immune sera from three continents indicates the potential of these proteins as vaccine candidate antigens. In order to characterize these exoantigens, a large-scale preparation of 5 liters of infected culture supernatant was fractionated by high resolution gel filtration, ammonium sulfate precipitation and preparative isoelectric focusing. Their isoelectric points were determined to range from 5.2-6.0. The partially purified fractions containing the high molecular weight antigens were resolved by SDS PAGE sequencing length gels, blotted onto activated glass fiber filters and individual bands submitted for amino-terminal sequencing. Unique amino-terminal sequences demonstrate that these proteins are a new group of previously uncharacterized exoantigens.
Download or read book Production and Characterization of Plasmodium Falciparum specific Monoclonal Antibodies written by P. K. C. Lim and published by . This book was released on 1998 with total page 2 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two monoclonal antibodies (Mabs), PF 2G4 and PF2G11 were produced by the hybridoma technique. Both Mabs were of the IgG1 subclass and were specific against soluble blood-stage antigens of Plasmodium falciparum in the ELISA. No cross reactions were observed with red blood cell antigens as well as solube antigens of other Plasmodium spp. like P. cynomolgi, p. inui. and berghei. Mab PF 2G4 recognized polypeptides of molecular weight 100 kilodaltons (kD) and a number of other polypeptides ranging from 38-50 kD in the immunoblot with P. falciparum antigens. Mab PF 2G11 also recognized a 100 kD polypeptide and 7 other polypeptides ranging from 38-45 kD. Both Mabs reacted with P. falciparum soluble antigens in the dot ELISA; the lowest concentration of antigen detected was 0.03 micrograms. [Authors' abstract].
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Malaria written by Institute of Medicine and published by National Academies Press. This book was released on 1991-02-01 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria is making a dramatic comeback in the world. The disease is the foremost health challenge in Africa south of the Sahara, and people traveling to malarious areas are at increased risk of malaria-related sickness and death. This book examines the prospects for bringing malaria under control, with specific recommendations for U.S. policy, directions for research and program funding, and appropriate roles for federal and international agencies and the medical and public health communities. The volume reports on the current status of malaria research, prevention, and control efforts worldwide. The authors present study results and commentary on the: Nature, clinical manifestations, diagnosis, and epidemiology of malaria. Biology of the malaria parasite and its vector. Prospects for developing malaria vaccines and improved treatments. Economic, social, and behavioral factors in malaria control.
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1991 with total page 1104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 742 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Expression Systems written by Michael R. Dyson and published by Methods Express (Hardcover). This book was released on 2007 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein expression is an increasingly important tool for research on gene function. What is needed is not just a lab manual providing established methods as well as the latest state-of-the-art protocols, but also clear advice on what expression system to choose when. Expression Systems: Methods Expressuniquely fills this need. It covers expression across a broad range of systems, including the following. *Baculovirus expression vectors *CHO cells *E. coli *HEK293-EBNA1 cells *Lactococcus lactis and other gram positive bacteria *S. cerevisiae *transfected insect cells *Pichia pastoris *mammalian cells using BacMam viruses *lentiviral vectors *wheat germ cell-free system The book takes the reader through how to make an informed choice of appropriate system, taking into account the protein target, the time involved, the ultimate use of the expressed protein, and the laboratory equipment required. It also provides step-by-step methods for each system. In addition, the book describes the optimisation of expression strategies, expression engineering using ribosome display, and how to select protein variants with improved expression. Every chapter discusses the merits and limitations of the approaches available, describes the key techniques in full practical detail, and provides sensible advice for immediate use at the bench. In summary, Expression Systems: Methods Expressis a comprehensive laboratory manual and information resource for researchers at all levels, from postgraduate student to principal investigator.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1836 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemical Protozoology As A Basis For Drug Design written by Graham H. Coombs and published by CRC Press. This book was released on 1991-10-07 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: A compilation of articles on protozoological biochemistry which reviews the subject area and offers information on current research. Included in the Topics Covered Are Energy Metabolism Of Anaerobic Parasitic Protists, proteinases of African
Download or read book Malaria written by Mats Wahlgren and published by CRC Press. This book was released on 2003-09-02 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Malaria causes more death and disease than any other parasitic pathogen known today. This multiauthored text covers the important areas of malaria research, particularly focusing on those sectors which are of clinical importance for the understanding of the disease, the parasite, and its vector. The chapter authors are all leading experts within their own particular fields. The biology and molecular biology of the parasite, the clinical spectrum of the disease, the pathogenesis of malaria, and the immunology and emergence of malaria vaccines are some examples of the scientific spheres that are discussed. The book is suitable as a text for graduate students and clinicians as well as researchers at universities and companies involved in treating or studying infectious diseases.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
